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Protein

Laccase-1

Gene

yA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for the conversion of the yellow polyketide pigment synthesized by wA to the conidial green pigment.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per subunit. The Cu cations are bound as 3 distinct Cu centers known as type 1 or blue, type 2 or normal, and type 3 or coupled binuclear.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Copper 1; type 2Curated1
Metal bindingi81Copper 2; type 3Curated1
Metal bindingi123Copper 2; type 3Curated1
Metal bindingi125Copper 3; type 3Curated1
Metal bindingi508Copper 4; type 1Curated1
Metal bindingi511Copper 1; type 2Curated1
Metal bindingi513Copper 3; type 3Curated1
Metal bindingi585Copper 3; type 3Curated1
Metal bindingi586Copper 4; type 1Curated1
Metal bindingi587Copper 2; type 3Curated1
Metal bindingi591Copper 4; type 1Curated1
Metal bindingi596Copper 4; type 1Curated1

GO - Molecular functioni

GO - Biological processi

  • conidium formation Source: AspGD
  • iron assimilation by reduction and transport Source: GO_Central
  • iron ion transmembrane transport Source: GOC
  • iron ion transport Source: GO_Central
  • pigment metabolic process involved in developmental pigmentation Source: AspGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processConidiation, Sporulation
LigandCopper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-1 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 1
Conidial laccase
Diphenol oxidase 1
Laccase I
Urishiol oxidase 1
Gene namesi
Name:yA
ORF Names:AN6635
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome I
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000292721 – 609Laccase-1Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi257N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi403N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi443N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi486N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi531N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi546N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Developmental stagei

Specifically expressed late during asexual development.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00007416

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 141Plastocyanin-like 1Add BLAST97
Domaini270 – 372Plastocyanin-like 2Add BLAST103
Domaini463 – 602Plastocyanin-like 3Add BLAST140

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000168741
InParanoidiP17489
OMAiCHITTHQ
OrthoDBiEOG092C0ZMK

Family and domain databases

Gene3Di2.60.40.4202 hits
InterProiView protein in InterPro
IPR001117 Cu-oxidase
IPR011706 Cu-oxidase_2
IPR011707 Cu-oxidase_3
IPR002355 Cu_oxidase_Cu_BS
IPR008972 Cupredoxin
PfamiView protein in Pfam
PF00394 Cu-oxidase, 1 hit
PF07731 Cu-oxidase_2, 1 hit
PF07732 Cu-oxidase_3, 1 hit
SUPFAMiSSF49503 SSF49503, 4 hits
PROSITEiView protein in PROSITE
PS00080 MULTICOPPER_OXIDASE2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLSTVLFPL LALNLGLSHA RFVRETLELT WEYGSPNGGT PREMVFTNGE
60 70 80 90 100
YPGPDLIFDE DDDVEVLVIN NLPFNTTVHW HGLEMRETPE ADGVPGLTQT
110 120 130 140 150
PIEPGATFTY RFRAYPAGTF WYHSHYKGLM QDGQVGAMYI RRKPDALRPY
160 170 180 190 200
AVITEDPREL AEIQYAEDNP YLMLATDWTY LTSAEYHNIE VESGYNVFCV
210 220 230 240 250
DSLLINGRGS VYCPGYQYLE EVSDDGLTAV LEGTHLTEKG CLQPDLHNVQ
260 270 280 290 300
GDYGPWNLSA VPTEVVFNCT PSSVEPPVIY VDPEFNGWVS LNFIGGAAQK
310 320 330 340 350
AITFSVDNHP MWVYEVDGQF VEPREVEMVG VYSGARYAVM IKLDQTPGDY
360 370 380 390 400
AIRIAVNGGD QVMSVYAILS YVNQDWIHRE NVPKAAIGPH TDTVGYMNYG
410 420 430 440 450
GGNTSADVRQ LLFTENLPAF GVPPPPPSSE VSTTLRTGMI RVNNSYSWSL
460 470 480 490 500
GNNVLYEPEM TSSTPLLFEP DPLAVIAPKY ALTTENNTWV DIVLEITADP
510 520 530 540 550
RDLIHPPHPI HKHGNRAYII GNGVGKFRWE NVSAAEAEVP DLFYVNETAA
560 570 580 590 600
LRDTFVTDFF DSRLMDGAWI VIRYFVQDKF PSILHCHIAS HQMGGMALAL

LDGVDVWDS
Length:609
Mass (Da):67,981
Last modified:May 26, 2009 - v3
Checksum:i529A20B2DD9AA73E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225 – 229DGLTA → TALLS in CAA36787 (PubMed:2192364).Curated5
Sequence conflicti359G → A in CAA36787 (PubMed:2192364).Curated1
Sequence conflicti421G → A in CAA36787 (PubMed:2192364).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52552 Genomic DNA Translation: CAA36787.1
AACD01000110 Genomic DNA Translation: EAA58164.1
BN001301 Genomic DNA Translation: CBF71142.1
PIRiS10149 KSASL1
RefSeqiXP_664239.1, XM_659147.1

Genome annotation databases

EnsemblFungiiCADANIAT00007416; CADANIAP00007416; CADANIAG00007416
EAA58164; EAA58164; AN6635.2
GeneIDi2870381
KEGGiani:AN6635.2

Similar proteinsi

Entry informationi

Entry nameiLAC1_EMENI
AccessioniPrimary (citable) accession number: P17489
Secondary accession number(s): C8V1C0, Q5AYJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 26, 2009
Last modified: February 28, 2018
This is version 124 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome