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P17468

- RDRP_ROTRF

UniProt

P17468 - RDRP_ROTRF

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsRNA formation. To do so, the polymerase specifically recognizes and binds 4 bases 5'-UGUG-3' in the conserved 3'-sequence of plus-strand RNA templates. VP2 presumably activates the autoinhibited VP1-RNA complex to coordinate packaging and genome replication. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription (By similarity).PROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Cofactori

Mg2+Curated

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Magnesium, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri10933 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000007179: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (By similarity).By similarity

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10881088RNA-directed RNA polymerasePRO_0000149525Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 (Potential). Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak (By similarity).By similarityCurated

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini501 – 687187RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17468-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MGKYNLILSE YLSFIYNSQS AVQIPIYYSS NSELENRCIE FHSKCLENSK
60 70 80 90 100
NGLSLKKLFV EYSDVMENAT LLSILSYSYD KYNAVERKLV KYAKGKPLEA
110 120 130 140 150
DLTVNELDYE NNKITSELFP TAEEYTDSLM DPAILTSLSS NLNAVMFWLE
160 170 180 190 200
KHENDVAEKL KIYKRRLDLF TIVASTVNKY GVPRHNAKYR YEYEVMKDKP
210 220 230 240 250
YYLVTWANSS IEMLMSVFSH EDYLIARELI VLSYSNRSTL AKLVSSPMSI
260 270 280 290 300
LVALVDINGT FITNEELELE FSNKYVRAIV PDQTFDELKQ MLDNMRKAGL
310 320 330 340 350
TDIPKMIQDW LVDCSIEKFP LMAKIYSWSF HVGFRKQKML DAALDQLKTE
360 370 380 390 400
YTEDVDDEMY REYTMLIRDE VVKMLEEPVK HDDHLLQDSE LAGLLSMSSA
410 420 430 440 450
SNGESRQLKF GRKTIFSTKK NMHVMDDMAN GRYTPGIIPP VNVDKPIPLG
460 470 480 490 500
RRDVPGRRTR IIFILPYEYF IAQHAVVEKM LIYAKHTREY AEFYSQSNQL
510 520 530 540 550
LSYGDVTRFL SNNSMVLYTD VSQWDSSQHN TQPFRKGIIM GLDMLANMTN
560 570 580 590 600
DARVIQTLNL YKQTQINLMD SYVQIPDGNV IKKIQYGAVA SGEKQTKAAN
610 620 630 640 650
SIANLALIKT VLSRISNKYS FATKIIRVDG DDNYAVLQFN TEVTEQMVQD
660 670 680 690 700
VSNDVRETYA RMNAKVKALV STVGIEIAKR YIAGGKIFFR AGINLLNNEK
710 720 730 740 750
RGQSTQWDQA AVLYSNYIVN RLRGFETDRE FILTKIMQMT SVAITGSLRL
760 770 780 790 800
FPSERVLTTN STFKVFDSED FIIEYGTTDD EVYIQRAFMS LSSQKSGIAD
810 820 830 840 850
EIAASSTFKN YVSRLSGQLL FSKNNIVSRG IALTEKAKLN SYAPISLEKR
860 870 880 890 900
RAQISALLTM LQKPVTFKSS KITINDILRD IKPFFTVNEA HLPIQYQKFM
910 920 930 940 950
PTLPDNVQYI IQCIGSRTYQ IEDDGSKSAI SRLISKYSVY KPSIEELYKV
960 970 980 990 1000
ISLHENEIQL YLISLGIPKI DADTYVGSKI YSQDKYRILE SYVCNLLSIN
1010 1020 1030 1040 1050
YGCYQLFDFN SPDLEKLIRI PFKGKIPAVT FILHLYAKLE VINHAIKNGS
1060 1070 1080
WISLFCNYPK SEMIKLWKKM WNITSLRSPY TNANFFQD
Length:1,088
Mass (Da):124,991
Last modified:August 1, 1990 - v1
Checksum:i2A449F7267CF7DF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04346 Genomic RNA. Translation: AAA47319.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04346 Genomic RNA. Translation: AAA47319.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of bovine rotavirus gene 1 and expression of the gene product in baculovirus."
    Cohen J., Charpilienne A., Chilmonczyk S., Estes M.K.
    Virology 171:131-140(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "The N terminus of rotavirus VP2 is necessary for encapsidation of VP1 and VP3."
    Zeng C.Q.-Y., Estes M.K., Charpilienne A., Cohen J.
    J. Virol. 72:201-208(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VP2 AND VP3.

Entry informationi

Entry nameiRDRP_ROTRF
AccessioniPrimary (citable) accession number: P17468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3