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P17467

- NSP5_ROTRA

UniProt

P17467 - NSP5_ROTRA

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Protein

Non-structural protein 5

Gene
N/A
Organism
Rotavirus A (strain Rabbit/United States/ALA/199? G3-P11[14]-Ix-Rx-Cx-Mx-A9-Nx-Tx-E5-H3) (RV-A) (Rotavirus A (strain Alabama))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Magnesium is required for ATPase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921MagnesiumSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: InterPro
  2. magnesium ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 5
Short name:
NSP5
Alternative name(s):
NS26
OrganismiRotavirus A (strain Rabbit/United States/ALA/199? G3-P11[14]-Ix-Rx-Cx-Mx-A9-Nx-Tx-E5-H3) (RV-A) (Rotavirus A (strain Alabama))
Taxonomic identifieri101359 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiOryctolagus cuniculus (Rabbit) [TaxID: 9986]

Subcellular locationi

Host cytoplasm
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Non-structural protein 5PRO_0000149640Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine; by host CK1By similarity
Modified residuei154 – 1541Phosphoserine; by hostBy similarity
Modified residuei156 – 1561Phosphoserine; by hostBy similarity
Modified residuei164 – 1641Phosphoserine; by hostBy similarity
Modified residuei166 – 1661Phosphoserine; by hostBy similarity

Post-translational modificationi

O-glycosylated.By similarity
Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4848Interaction with VP1By similarityAdd
BLAST
Regioni189 – 19810Homodimerization and interaction with NSP6By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 101100Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP5 family.Curated

Family and domain databases

InterProiIPR002512. Rotavirus_A/C_NSP5.
[Graphical view]
PfamiPF01525. Rota_NS26. 1 hit.
[Graphical view]
PIRSFiPIRSF004006. Rota_NS26. 1 hit.

Sequencei

Sequence statusi: Complete.

P17467-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLSIDVTSL PSISSSIFKN KSSSTASTLS GKSIGRNELY VSPDAEAFNK
60 70 80 90 100
YMLSKSPEDI GPSDSASNDP LTSFSIRSHA VKTNADAGVS MDSSTQSRPS
110 120 130 140 150
SNVGCDKVDF SFNKAMKVNA NLDLSISIST DQKREKSKKD HKNKKCYPKI
160 170 180 190
EAESDSDDYV LDDSDSDDGK CKNCKYKRKY FALRMRMKHV AMQLIEDL
Length:198
Mass (Da):21,810
Last modified:August 1, 1990 - v1
Checksum:i3CC6621043E4DCEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04361 Genomic DNA. Translation: AAA66883.1.
PIRiA30233. MNXRRA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04361 Genomic DNA. Translation: AAA66883.1 .
PIRi A30233. MNXRRA.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002512. Rotavirus_A/C_NSP5.
[Graphical view ]
Pfami PF01525. Rota_NS26. 1 hit.
[Graphical view ]
PIRSFi PIRSF004006. Rota_NS26. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evidence of duplication and deletion in super short segment 11 of rabbit rotavirus Alabama strain."
    Gorziglia M., Nishikawa K., Fukuhara N.
    Virology 170:587-590(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiNSP5_ROTRA
AccessioniPrimary (citable) accession number: P17467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 26, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3