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P17462

- VP2_ROTBU

UniProt

P17462 - VP2_ROTBU

Protein

Inner capsid protein VP2

Gene
N/A
Organism
Rotavirus A (strain Cow/United Kingdom/UK/1975 G6-P7[5]-I2-R2-C2-M2-A3-N2-T7-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inner capsid protein VP2
    OrganismiRotavirus A (strain Cow/United Kingdom/UK/1975 G6-P7[5]-I2-R2-C2-M2-A3-N2-T7-E2-H3) (RV-A)
    Taxonomic identifieri10934 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000008657: Genome

    Subcellular locationi

    Virion Curated
    Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.By similarity

    GO - Cellular componenti

    1. viral inner capsid Source: UniProtKB-KW
    2. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Capsid protein, Inner capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 881881Inner capsid protein VP2PRO_0000149532Add
    BLAST

    Proteomic databases

    PRIDEiP17462.

    Interactioni

    Subunit structurei

    Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.By similarityCurated

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3KZ4X-ray3.80A/B1-881[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 9292Interaction with VP1 and VP3By similarityAdd
    BLAST
    Regioni394 – 41421HydrophobicAdd
    BLAST
    Regioni422 – 44221HydrophobicAdd
    BLAST
    Regioni537 – 55822Leucine-zipper 1Add
    BLAST
    Regioni664 – 68724Leucine-zipper 2Add
    BLAST

    Domaini

    The N-terminus is involved in VP1 binding.

    Sequence similaritiesi

    Belongs to the rotavirus VP2 family.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    InterProiIPR007779. Rotavirus_VP2.
    [Graphical view]
    PfamiPF05087. Rota_VP2. 1 hit.
    [Graphical view]
    ProDomiPD008866. Rota_VP2. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P17462-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYRKRGATV EADINNNDRM QEKDDEKQDQ NNRMQLSDKV LSKKEEVVTD    50
    SQEEIKIRDE VKKSTKEESK QLLEVLKTKE EHQKEIQYEI LQKTIPTFEP 100
    KESILKKLED IKPEQAKKQT KLFRIFEPRQ LPIYRANGEK ELRNRWYWKL 150
    KKDTLPDGDY DVREYFLNLY DQVLTEMPDY LLLKDMAVEN KNSRDAGKVV 200
    DSETASICDA IFQDEETEGA VRRFIAEMRQ RVQADRNVVN YPSILHPIDY 250
    AFNEYFLQHQ LVEPLNNDII FNYIPERIRN DVNYILNMDR NLPSTARYIR 300
    PNLLQDRLNL HDNFESLWDT ITTSNYILAR SVVPDLKELV STEAQIQKMS 350
    QDLQLEALTI QSETQFLTGI NSQAANDCFK TLIAAMLSQR TMSLDFVTTN 400
    YMSLISGMWL LTVVPNDMFI RESLVACQLA IVNTIIYPAF GMQRMHYRNG 450
    DPQTPFQIAE QQIRKFSGSG IGWHFVNNNQ FRQVVIDGVL NQVLNDNIRN 500
    VHVIKQLMQA LMQLSRQQFP TMPVDYKRSI QRGILLLSNR LGQLVDLTRL 550
    LAYNYETLMA CVTMNMQHVQ TLTTEKLQLT SVTSLCMLIG NATVIPSPQT 600
    LFHYYNVNVN FHSNYNERIN DAVAIITAAN RLNLYQKKMK AIVEDFLKRL 650
    HIFDVARVPD DQMYRLRDRL RLLPVEVRRL DIFNLILMNM DQIERASDKI 700
    AQGVIIAYRD MQLERDEMYG YVNIARNLDG FQQINLEELM RTGDYAQITN 750
    MLLNNQPVAL VGALPFVTDS SVISLIAKLD ATVFAQIVKL RKVDTLKPIL 800
    YKINSDSNDF YLVANYDWVP TSTTKVYKQV PQQFDFRNSM HMLTSNLTFT 850
    VYSDLLAFVS ADTVEPINAV AFDNMRIMNE L 881
    Length:881
    Mass (Da):102,488
    Last modified:August 1, 1990 - v1
    Checksum:i7CFCDF653E4CF2CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52589 Genomic RNA. Translation: CAA36825.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52589 Genomic RNA. Translation: CAA36825.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3KZ4 X-ray 3.80 A/B 1-881 [» ]
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P17462.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007779. Rotavirus_VP2.
    [Graphical view ]
    Pfami PF05087. Rota_VP2. 1 hit.
    [Graphical view ]
    ProDomi PD008866. Rota_VP2. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of gene 2 of the UK tissue culture adapted strain of bovine rotavirus."
      Tian Y., Tarlow O., McCrae M.A.
      Nucleic Acids Res. 18:4015-4015(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiVP2_ROTBU
    AccessioniPrimary (citable) accession number: P17462
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3