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P17462

- VP2_ROTBU

UniProt

P17462 - VP2_ROTBU

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Protein
Inner capsid protein VP2
Gene
N/A
Organism
Rotavirus A (strain Cow/United Kingdom/UK/1975 G6-P7[5]-I2-R2-C2-M2-A3-N2-T7-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inner capsid protein VP2
OrganismiRotavirus A (strain Cow/United Kingdom/UK/1975 G6-P7[5]-I2-R2-C2-M2-A3-N2-T7-E2-H3) (RV-A)
Taxonomic identifieri10934 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000008657: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

GO - Cellular componenti

  1. viral inner capsid Source: UniProtKB-KW
  2. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Inner capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 881881Inner capsid protein VP2
PRO_0000149532Add
BLAST

Proteomic databases

PRIDEiP17462.

Interactioni

Subunit structurei

Dimer Reviewed prediction. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KZ4X-ray3.80A/B1-881[»]

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9292Interaction with VP1 and VP3 By similarity
Add
BLAST
Regioni394 – 41421Hydrophobic
Add
BLAST
Regioni422 – 44221Hydrophobic
Add
BLAST
Regioni537 – 55822Leucine-zipper 1
Add
BLAST
Regioni664 – 68724Leucine-zipper 2
Add
BLAST

Domaini

The N-terminus is involved in VP1 binding.

Sequence similaritiesi

Belongs to the rotavirus VP2 family.

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR007779. Rotavirus_VP2.
[Graphical view]
PfamiPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomiPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P17462-1 [UniParc]FASTAAdd to Basket

« Hide

MAYRKRGATV EADINNNDRM QEKDDEKQDQ NNRMQLSDKV LSKKEEVVTD    50
SQEEIKIRDE VKKSTKEESK QLLEVLKTKE EHQKEIQYEI LQKTIPTFEP 100
KESILKKLED IKPEQAKKQT KLFRIFEPRQ LPIYRANGEK ELRNRWYWKL 150
KKDTLPDGDY DVREYFLNLY DQVLTEMPDY LLLKDMAVEN KNSRDAGKVV 200
DSETASICDA IFQDEETEGA VRRFIAEMRQ RVQADRNVVN YPSILHPIDY 250
AFNEYFLQHQ LVEPLNNDII FNYIPERIRN DVNYILNMDR NLPSTARYIR 300
PNLLQDRLNL HDNFESLWDT ITTSNYILAR SVVPDLKELV STEAQIQKMS 350
QDLQLEALTI QSETQFLTGI NSQAANDCFK TLIAAMLSQR TMSLDFVTTN 400
YMSLISGMWL LTVVPNDMFI RESLVACQLA IVNTIIYPAF GMQRMHYRNG 450
DPQTPFQIAE QQIRKFSGSG IGWHFVNNNQ FRQVVIDGVL NQVLNDNIRN 500
VHVIKQLMQA LMQLSRQQFP TMPVDYKRSI QRGILLLSNR LGQLVDLTRL 550
LAYNYETLMA CVTMNMQHVQ TLTTEKLQLT SVTSLCMLIG NATVIPSPQT 600
LFHYYNVNVN FHSNYNERIN DAVAIITAAN RLNLYQKKMK AIVEDFLKRL 650
HIFDVARVPD DQMYRLRDRL RLLPVEVRRL DIFNLILMNM DQIERASDKI 700
AQGVIIAYRD MQLERDEMYG YVNIARNLDG FQQINLEELM RTGDYAQITN 750
MLLNNQPVAL VGALPFVTDS SVISLIAKLD ATVFAQIVKL RKVDTLKPIL 800
YKINSDSNDF YLVANYDWVP TSTTKVYKQV PQQFDFRNSM HMLTSNLTFT 850
VYSDLLAFVS ADTVEPINAV AFDNMRIMNE L 881
Length:881
Mass (Da):102,488
Last modified:August 1, 1990 - v1
Checksum:i7CFCDF653E4CF2CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52589 Genomic RNA. Translation: CAA36825.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52589 Genomic RNA. Translation: CAA36825.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3KZ4 X-ray 3.80 A/B 1-881 [» ]
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P17462.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007779. Rotavirus_VP2.
[Graphical view ]
Pfami PF05087. Rota_VP2. 1 hit.
[Graphical view ]
ProDomi PD008866. Rota_VP2. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of gene 2 of the UK tissue culture adapted strain of bovine rotavirus."
    Tian Y., Tarlow O., McCrae M.A.
    Nucleic Acids Res. 18:4015-4015(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP2_ROTBU
AccessioniPrimary (citable) accession number: P17462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 16, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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