ID   RDRP_TCV                Reviewed;         775 AA.
AC   P17460; Q7TD17; Q7TD21; Q88486;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 3.
DT   13-SEP-2023, entry version 95.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p88;
DE   Contains:
DE     RecName: Full=Protein p28;
GN   ORFNames=ORF1;
OS   Turnip crinkle virus (TCV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Betacarmovirus;
OC   Betacarmovirus brassicae.
OX   NCBI_TaxID=11988;
OH   NCBI_TaxID=3709; Brassica napus subsp. rapifera.
OH   NCBI_TaxID=5130; Hypomyces.
OH   NCBI_TaxID=180540; Moricandia arvensis (Purple mistress) (Brassica arvensis).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2718381; DOI=10.1016/0042-6822(89)90369-3;
RA   Carrington J.C., Heaton L.A., Zuidema D., Hillman B.I., Morris T.J.;
RT   "The genome structure of turnip crinkle virus.";
RL   Virology 170:219-226(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Infectious clone UK;
RA   Ryabov E.V.;
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   SEQUENCE REVISION.
RA   Carrington J.C., Heaton L.A., Zuidema D., Hillman B.I., Morris T.J.;
RL   Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       251.
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DR   EMBL; M22445; AAA96968.2; -; Genomic_RNA.
DR   EMBL; M22445; AAA96969.3; -; Genomic_RNA.
DR   EMBL; AY312063; AAP78490.1; -; Genomic_RNA.
DR   EMBL; AY312063; AAP78486.1; -; Genomic_RNA.
DR   PIR; JA0110; RRVETC.
DR   RefSeq; NP_620720.3; NC_003821.3.
DR   RefSeq; NP_620721.2; NC_003821.3.
DR   GeneID; 944391; -.
DR   GeneID; 944392; -.
DR   KEGG; vg:944391; -.
DR   KEGG; vg:944392; -.
DR   OrthoDB; 12338at10239; -.
DR   Proteomes; UP000007403; Genome.
DR   Proteomes; UP000009133; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   CDD; cd23240; Betacarmovirus_RdRp; 1.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   Pfam; PF00998; RdRP_3; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   4: Predicted;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA suppression of termination; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..775
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000040204"
FT   CHAIN           1..250
FT                   /note="Protein p28"
FT                   /id="PRO_0000040205"
FT   DOMAIN          473..586
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   VARIANT         42
FT                   /note="R -> Q (in strain: Infectious clone UK)"
FT   VARIANT         56
FT                   /note="D -> S (in strain: Infectious clone UK)"
FT   VARIANT         202
FT                   /note="D -> N (in strain: Infectious clone UK)"
FT   VARIANT         345
FT                   /note="R -> K (in strain: Infectious clone UK)"
FT   VARIANT         581
FT                   /note="D -> E (in strain: Infectious clone UK)"
FT   VARIANT         717
FT                   /note="D -> N (in strain: Infectious clone UK)"
FT   VARIANT         748
FT                   /note="Q -> E"
SQ   SEQUENCE   775 AA;  87648 MW;  A19CE0848C75253F CRC64;
     MPLLHTLNTA LAVGLLGARY YPEVQTFLGL PDYVGHMKNV VRSVFQGSGL VVVSSDTVGV
     RGTYSNRGQI GSSLGCILAV PDSGADIEID LDRLVGTEEE ATSCLVEAVG STADVPRRRV
     RQKGRFAMHA VNAAKLHFCG VPKPTEANRL AVSKWLVQYC KERHVVDSHI RTIVNTALPR
     VFTPDAEDIQ VVLDLHSVRA HDHRNALAEA GKVRKWWVNL AMHPMTGRSW SRAWRRLCRL
     PDDQAISFVR XGCLRELVGR ETQISRGENP AMRVFPLANP PKVRRIFHIC GMGNGLDFGV
     HNNSLNNLRR GLMERVFYVE DAQKQLKPAP QPIPGIFGKL SGIRRRLVRL AGNHTPVPRE
     KYPSFYKGRR ATIYQKALDS LHDRPVSRKD AELKTFVKAE KINFTAKKDP APRVIQPRDP
     RYNIEVGKYL KPYEHHLYRA IDAMWGGPTV LKGYDVGELG NIMSNTWDKF RKTCAIGFDM
     KRFDQHVSVD ALRWEHSVYN AGFNCPELAQ LLTWQLTNKG VGRASDGFIK YQVDGCRMSG
     DVNTALGNCL LACSITKYLM KGIKCKLINN GDDCVLFFEA DEVDRVRERL HHWIDFGFQC
     IAEEPQYELE KVEFCQMSPI FDGEGWVMVR NPRVSLSKDS YSTTQWANEK DAARWLAAIG
     ECGLAIAGGV PVLQSYYSCL KRNFGPLAGD YKKKMQDVSF DSGFYRLSKN GMRGSKDVSQ
     DARFSFYRGF GYTPDEQEAL EEYYDNLQLL CEWDPTGYKE ELSDRWILNE FPTTL
//