Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dermonecrotic toxin

Gene

toxA

Organism
Pasteurella multocida
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs.

GO - Molecular functioni

  • phospholipase activity Source: AgBase
  • phospholipid binding Source: AgBase

GO - Biological processi

  • killing by symbiont of host cells Source: CACAO
  • positive regulation by symbiont of host transcription Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Protein family/group databases

TCDBi1.C.57.3.1. the clostridial cytotoxin (cct) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Dermonecrotic toxin
Short name:
DNT
Alternative name(s):
Mitogenic toxin
PMT
Gene namesi
Name:toxA
OrganismiPasteurella multocida
Taxonomic identifieri747 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei402 – 422HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB-SubCell
  • host cell plasma membrane Source: AgBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Host membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726351 – 1285Dermonecrotic toxinAdd BLAST1285

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
VimP201524EBI-9541048,EBI-299269From a different organism.

Protein-protein interaction databases

IntActiP17452. 2 interactors.

Structurei

Secondary structure

11285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi578 – 580Combined sources3
Helixi593 – 599Combined sources7
Helixi609 – 623Combined sources15
Helixi627 – 647Combined sources21
Helixi654 – 668Combined sources15
Helixi671 – 673Combined sources3
Helixi674 – 682Combined sources9
Helixi690 – 702Combined sources13
Helixi710 – 719Combined sources10
Turni721 – 724Combined sources4
Beta strandi744 – 747Combined sources4
Helixi749 – 753Combined sources5
Beta strandi754 – 756Combined sources3
Turni759 – 761Combined sources3
Helixi763 – 765Combined sources3
Turni766 – 769Combined sources4
Helixi773 – 787Combined sources15
Beta strandi788 – 790Combined sources3
Beta strandi793 – 796Combined sources4
Beta strandi799 – 802Combined sources4
Helixi806 – 812Combined sources7
Turni815 – 817Combined sources3
Beta strandi822 – 824Combined sources3
Helixi827 – 839Combined sources13
Helixi844 – 848Combined sources5
Helixi851 – 872Combined sources22
Helixi878 – 884Combined sources7
Helixi889 – 895Combined sources7
Helixi898 – 907Combined sources10
Beta strandi910 – 916Combined sources7
Helixi918 – 926Combined sources9
Helixi928 – 933Combined sources6
Beta strandi938 – 945Combined sources8
Helixi946 – 957Combined sources12
Helixi964 – 973Combined sources10
Turni974 – 976Combined sources3
Helixi981 – 991Combined sources11
Beta strandi995 – 1000Combined sources6
Helixi1012 – 1029Combined sources18
Beta strandi1037 – 1041Combined sources5
Helixi1043 – 1046Combined sources4
Beta strandi1049 – 1056Combined sources8
Helixi1059 – 1063Combined sources5
Beta strandi1067 – 1070Combined sources4
Beta strandi1076 – 1078Combined sources3
Helixi1083 – 1085Combined sources3
Beta strandi1086 – 1088Combined sources3
Beta strandi1103 – 1107Combined sources5
Beta strandi1110 – 1112Combined sources3
Beta strandi1116 – 1119Combined sources4
Helixi1122 – 1130Combined sources9
Helixi1135 – 1149Combined sources15
Helixi1152 – 1156Combined sources5
Turni1162 – 1164Combined sources3
Helixi1168 – 1178Combined sources11
Beta strandi1187 – 1195Combined sources9
Turni1196 – 1198Combined sources3
Beta strandi1199 – 1212Combined sources14
Beta strandi1215 – 1220Combined sources6
Helixi1223 – 1225Combined sources3
Beta strandi1230 – 1232Combined sources3
Beta strandi1234 – 1239Combined sources6
Helixi1240 – 1250Combined sources11
Beta strandi1254 – 1261Combined sources8
Helixi1265 – 1269Combined sources5
Helixi1275 – 1277Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EBFX-ray1.90X569-1285[»]
2EBHX-ray2.40X569-1285[»]
2EC5X-ray2.60A/B569-1285[»]
ProteinModelPortaliP17452.
SMRiP17452.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17452.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR020972. Dermonecrotic/RTX_toxin_C.
[Graphical view]
PfamiPF11647. PMT_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17452-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTKHFFNSD FTVKGKSADE IFRRLCTDHP DKQLNNVKWK EVFINRFGQM
60 70 80 90 100
MLDTPNPRKI VEKIINEGLE KQGLKNIDPE TTYFNIFSSS DSSDGNVFHY
110 120 130 140 150
NSLSESYRVT DACLMNIFVE RYFDDWDLLN SLASNGIYSV GKEGAYYPDH
160 170 180 190 200
DYGPEYNPVW GPNEQIYHSR VIADILYARS VWDEFKKYFM EYWQKYAQLY
210 220 230 240 250
TEMLSDTFLA MAIQQYTRQT LTDEGFLMVC NTYYGNKEEV QITLLDIYGY
260 270 280 290 300
PSTDIICIEQ KGLPTPKVIL YIPGGTQPFV EFLNTDDLKQ WIAWHLKDNK
310 320 330 340 350
HMVAFRKHFS LKQRQEGETF TGIDKALQYI AEESPEWPAN KYILYNPTHL
360 370 380 390 400
ETENLFNIMM KRTEQRMLED SDVQIRSNSE ATRDYALSLL ETFISQLSAI
410 420 430 440 450
DMLVPAVGIP INFALSATAL GLSSDIVVNG DSYEKRKYGI GSLVQSALFT
460 470 480 490 500
GINLIPVISE TAEILSSFSR TEEDIPAFFT EEQALAQRFE IVEEELHSIS
510 520 530 540 550
PDDPPREITD ENLHKIRLVR LNNENQPLVV LRRLGGNKFI RIEPITFQEI
560 570 580 590 600
KGSLVSEVIN PVTNKTYYVS NAKLLGGSPY SPFRIGLEGV WTPEVLKARA
610 620 630 640 650
SVIGKPIGES YKRILAKLQR IHNSNILDER QGLMHELMEL IDLYEESQPS
660 670 680 690 700
SERLNAFREL RTQLEKALYL PEMEALKKQI LQIPNKGSGA ARFLLRTAMN
710 720 730 740 750
EMAGKTSEST ADLIRFALQD TVISAPFRGY AGAIPEAIDF PVKYVIEDIS
760 770 780 790 800
VFDKIQTNYW ELPAYESWNE GSNSALLPGL LRESQSKGML SKCRIIENSL
810 820 830 840 850
YIGHSYEEMF YSISPYSNQV GGPYELYPFT FFSMLQEVQG DLGFEQAFAT
860 870 880 890 900
RNFFNTLVSD RLSLMENTML LTESFDYTPW DAIYGDINYD EQFAAMSINE
910 920 930 940 950
RIEKCMNTYR GVAFQNSSKS IDFFLNNLTT FIDNGLTEIA ISDLPYDIVQ
960 970 980 990 1000
QEISQFLQGS NEWKTLDAML FNLDKGDING AFRKLLQSAK DNNIKFRAIG
1010 1020 1030 1040 1050
HSDNSVPPFN NPYKSLYYKG NIIAEAIEKL DREGQKFVVF ADSSLLNSTP
1060 1070 1080 1090 1100
GTGRPMPGLV QYLKIPATVV DSDGAWQFLP DVASSRVPIE VTELENWQVL
1110 1120 1130 1140 1150
TPPQGKILGL KQFKLTAGFP TEQSRLPLLE NSVSEDLREE LMQKIDAIKN
1160 1170 1180 1190 1200
DVKMNSLVCM EAGSCDSVSP KVAARLKDMG LEAGMGASIT WWRREGGMEF
1210 1220 1230 1240 1250
SHQMHTTASF KFAGKEFAVD ASHLQFVHDQ LDTTILILPV DDWALEIAQR
1260 1270 1280
NRAINPFVEY VSKTGNMLAL FMPPLFTKPR LTRAL
Length:1,285
Mass (Da):146,383
Last modified:April 27, 2001 - v2
Checksum:iE67A9FCA58C107DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti304A → R in CAA35885 (PubMed:2201870).Curated1
Sequence conflicti775A → R in CAA35885 (PubMed:2201870).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti853F → Y in strain: CVI 47459. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51512 Genomic DNA. Translation: CAA35885.1.
X52478 Genomic DNA. Translation: CAA36717.1.
Z28388 Genomic DNA. Translation: CAA82233.1.
X57775 Genomic DNA. Translation: CAA40921.1.
AF240778 Genomic DNA. Translation: AAL55665.1.
PIRiS12998. BTQPD.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51512 Genomic DNA. Translation: CAA35885.1.
X52478 Genomic DNA. Translation: CAA36717.1.
Z28388 Genomic DNA. Translation: CAA82233.1.
X57775 Genomic DNA. Translation: CAA40921.1.
AF240778 Genomic DNA. Translation: AAL55665.1.
PIRiS12998. BTQPD.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EBFX-ray1.90X569-1285[»]
2EBHX-ray2.40X569-1285[»]
2EC5X-ray2.60A/B569-1285[»]
ProteinModelPortaliP17452.
SMRiP17452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP17452. 2 interactors.

Protein family/group databases

TCDBi1.C.57.3.1. the clostridial cytotoxin (cct) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP17452.

Family and domain databases

InterProiIPR020972. Dermonecrotic/RTX_toxin_C.
[Graphical view]
PfamiPF11647. PMT_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOXA_PASMD
AccessioniPrimary (citable) accession number: P17452
Secondary accession number(s): Q57008
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Closely spaced cysteine and histidine residues may provide the toxin with an affinity for metal.
The sequence shown is that of strain NCTC 12178.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.