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P17450 (SPVB_SALCH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Mono(ADP-ribosyl)transferase SpvB
Short name=mADPRT
Short name=mART
EC=2.4.2.31
Alternative name(s):
65 kDa virulence protein
NAD(+)--arginine ADP-ribosyltransferase
Protein M2 in mba region
Toxin SpvB
Gene names
Name:spvB
Ordered Locus Names:SCH_V04
Encoded onPlasmid pKDSc50 Ref.1 Ref.2
Plasmid pSCV50 Ref.3
OrganismSalmonella choleraesuis (strain SC-B67) [Complete proteome] [HAMAP]
Taxonomic identifier321314 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length591 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Mono-ADP-ribosylates eukaryotic muscle and non-muscle actin on 'Arg-177'. ADP-ribosylation prevents the polymerization of G-actin to F-actin, causing actin filament depolymerization, destruction of the cytoskeleton and cytotoxicity. Does not possess NAD+-glycohydrolase activity, unlike most mART enzymes By similarity.

Catalytic activity

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.

NADP+ + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.

Subcellular location

Secreted. Note: Secreted via the type III secretion system 1 (SPI-2 TTSS) By similarity.

Miscellaneous

In Salmonella spp. the spv gene cluster is encoded on a highly transmissible plasmid.

Sequence similarities

Belongs to the SpvB family.

Ontologies

Keywords
   Biological processVirulence
   Cellular componentSecreted
   LigandNAD
NADP
Nucleotide-binding
   Molecular functionNucleotidyltransferase
Toxin
Transferase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNAD(P)+-protein-arginine ADP-ribosyltransferase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotidyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 591591Mono(ADP-ribosyl)transferase SpvB
PRO_0000221663

Regions

Compositional bias367 – 3737Poly-Pro

Sites

Active site4711 By similarity
Active site5381 By similarity

Experimental info

Sequence conflict1181C → S in CAA36278. Ref.1
Sequence conflict1181C → S in BAB20511. Ref.2
Sequence conflict4031A → R in CAA36278. Ref.1
Sequence conflict4031A → R in BAB20511. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P17450 [UniParc].

Last modified June 7, 2005. Version 2.
Checksum: 16030F6BAA21F813

FASTA59165,272
        10         20         30         40         50         60 
MLILNGFSSA TLALITPPSL PKGGKALSQS GPDGLASITL PLPISAERGF APALALHYSS 

        70         80         90        100        110        120 
GGGNGPFGVG WSCATMSIAR STSHGVPQYN DSDEFLGPDG EVLVQTLSTG DAPNPVTCFA 

       130        140        150        160        170        180 
YGDVSFPQSY TVTRYQPRTE SSFYRLEYWV GNSNGDDFWL LHDSNGILHL LGKTAAARLS 

       190        200        210        220        230        240 
DPQAASHTAQ WLVEESVTPA GEHIYYSYLA ENGDNVDLNG NEAGRDRSAM RYLSKVQYGN 

       250        260        270        280        290        300 
ATPAADLYLW TSATPAVQWL FTLVFDYGER GVDPQVPPAF TAQNSWLARQ DPFSLYNYGF 

       310        320        330        340        350        360 
EIRLHRLCRQ VLMFHHFPDE LGEADTLVSR LLLEYDENPI LTQLCAARTL AYEGDGYRRA 

       370        380        390        400        410        420 
PVNNIMPPPP PPPMMGGNSS RPKSKWAIVE ESKQIQALRY YSAQGYSVIN KYLRGDDYPE 

       430        440        450        460        470        480 
TQAKETLLSR DYLSTNEPSD EEFKNAMSVY INDIAEGLSS LPETDHRVVY RGLKLDKPAL 

       490        500        510        520        530        540 
SDVLKEYTTI GNIIIDKAFM STSPDKAWIN DTILNIYLEK GHKGRILGDV AHFKGEAEML 

       550        560        570        580        590 
FPPNTKLKIE SIVNCGSQDF ASQLSKLRLS DDATADTNRI KRIINMRVLN S

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequences of genes encoding 32 kDa and 70 kDa polypeptides in mba region of the virulence plasmid, pKDSc50, of Salmonella choleraesuis."
Matsui H.
Nucleic Acids Res. 18:2181-2181(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RF-1.
[2]"Complete DNA sequence and comparative analysis of the 50-kilobase virulence plasmid of Salmonella enterica serovar Choleraesuis."
Haneda T., Okada N., Nakazawa N., Kawakami T., Danbara H.
Infect. Immun. 69:2612-2620(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RF-1.
[3]"The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen."
Chiu C.-H., Tang P., Chu C., Hu S., Bao Q., Yu J., Chou Y.-Y., Wang H.-S., Lee Y.-S.
Nucleic Acids Res. 33:1690-1698(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC-B67.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X52035 Genomic DNA. Translation: CAA36278.1.
AB040415 Genomic DNA. Translation: BAB20511.1.
AY509003 Genomic DNA. Translation: AAS58877.1.
PIRS09498.
RefSeqYP_209258.1. NC_006855.1.

3D structure databases

ProteinModelPortalP17450.
SMRP17450. Positions 391-590.
ModBaseSearch...

Protein-protein interaction databases

STRING321314.SCV04.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS58877; AAS58877; SCH_V04.
GeneID3283353.
KEGGsec:SCV04.
PATRIC32319955. VBISalEnt136302_0007.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK15366.
OMAAWINDTI.
ProtClustDBPRK15244.

Enzyme and pathway databases

BioCycSENT321314:GJCS-4836-MONOMER.

Family and domain databases

InterProIPR003540. ADP-ribosyltransferase.
IPR003284. Sal_SpvB.
[Graphical view]
PfamPF03496. ADPrib_exo_Tox. 1 hit.
[Graphical view]
PRINTSPR01341. SALSPVBPROT.
ProtoNetSearch...

Entry information

Entry nameSPVB_SALCH
AccessionPrimary (citable) accession number: P17450
Secondary accession number(s): Q5J4C8, Q7DIJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: June 7, 2005
Last modified: May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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