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Protein

HTH-type transcriptional regulator BetI

Gene

betI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Repressor involved in the biosynthesis of the osmoprotectant glycine betaine. It represses transcription of the choline transporter BetT and the genes of BetAB involved in the synthesis of glycine betaine.2 Publications

Miscellaneous

BetI is an unusual repressor because it remains bound to DNA during induction.1 Publication

Pathwayi: betaine biosynthesis via choline pathway

This protein regulates the pathway betaine biosynthesis via choline pathway, which is part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi31 – 50H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • response to osmotic stress Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00251.
UniPathwayiUPA00529.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator BetIUniRule annotation
Gene namesi
Name:betIUniRule annotation
Ordered Locus Names:b0313, JW0305
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10111. betI.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

In cells lacking this gene, expression of the bet promoters is stimulated by osmotic stress but not by choline.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000705801 – 195HTH-type transcriptional regulator BetIAdd BLAST195

Proteomic databases

PaxDbiP17446.
PRIDEiP17446.

Expressioni

Inductioni

Negatively autoregulated.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262808. 6 interactors.
IntActiP17446. 10 interactors.
STRINGi511145.b0313.

Structurei

3D structure databases

ProteinModelPortaliP17446.
SMRiP17446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 68HTH tetR-typeUniRule annotationAdd BLAST61

Phylogenomic databases

eggNOGiENOG4108MHR. Bacteria.
COG1309. LUCA.
HOGENOMiHOG000248227.
InParanoidiP17446.
KOiK02167.
PhylomeDBiP17446.

Family and domain databases

HAMAPiMF_00768. HTH_type_BetI. 1 hit.
InterProiView protein in InterPro
IPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeobox-like.
IPR001647. HTH_TetR.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR017757. Tscrpt_rep_BetI.
PfamiView protein in Pfam
PF00440. TetR_N. 1 hit.
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
TIGRFAMsiTIGR03384. betaine_BetI. 1 hit.
PROSITEiView protein in PROSITE
PS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P17446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKLGMQSIR RRQLIDATLE AINEVGMHDA TIAQIARRAG VSTGIISHYF
60 70 80 90 100
RDKNGLLEAT MRDITSQLRD AVLNRLHALP QGSAEQRLQA IVGGNFDETQ
110 120 130 140 150
VSSAAMKAWL AFWASSMHQP MLYRLQQVSS RRLLSNLVSE FRRELPREQA
160 170 180 190
QEAGYGLAAL IDGLWLRAAL SGKPLDKTRA NSLTRHFITQ HLPTD
Length:195
Mass (Da):21,815
Last modified:August 1, 1990 - v1
Checksum:iB52A77B3A605E354
GO

Sequence cautioni

The sequence AAB18039 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52905 Genomic DNA. Translation: CAA37091.1.
U73857 Genomic DNA. Translation: AAB18039.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73416.1.
AP009048 Genomic DNA. Translation: BAE76096.1.
PIRiS15180. S10899.
RefSeqiNP_414847.3. NC_000913.3.
WP_001335745.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73416; AAC73416; b0313.
BAE76096; BAE76096; BAE76096.
GeneIDi944981.
KEGGiecj:JW0305.
eco:b0313.
PATRICifig|1411691.4.peg.1964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52905 Genomic DNA. Translation: CAA37091.1.
U73857 Genomic DNA. Translation: AAB18039.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73416.1.
AP009048 Genomic DNA. Translation: BAE76096.1.
PIRiS15180. S10899.
RefSeqiNP_414847.3. NC_000913.3.
WP_001335745.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP17446.
SMRiP17446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262808. 6 interactors.
IntActiP17446. 10 interactors.
STRINGi511145.b0313.

Proteomic databases

PaxDbiP17446.
PRIDEiP17446.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73416; AAC73416; b0313.
BAE76096; BAE76096; BAE76096.
GeneIDi944981.
KEGGiecj:JW0305.
eco:b0313.
PATRICifig|1411691.4.peg.1964.

Organism-specific databases

EchoBASEiEB0109.
EcoGeneiEG10111. betI.

Phylogenomic databases

eggNOGiENOG4108MHR. Bacteria.
COG1309. LUCA.
HOGENOMiHOG000248227.
InParanoidiP17446.
KOiK02167.
PhylomeDBiP17446.

Enzyme and pathway databases

UniPathwayiUPA00529.
BioCyciEcoCyc:PD00251.

Miscellaneous databases

PROiPR:P17446.

Family and domain databases

HAMAPiMF_00768. HTH_type_BetI. 1 hit.
InterProiView protein in InterPro
IPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeobox-like.
IPR001647. HTH_TetR.
IPR011075. Tet_transcr_reg_TetR-rel_C.
IPR017757. Tscrpt_rep_BetI.
PfamiView protein in Pfam
PF00440. TetR_N. 1 hit.
PRINTSiPR00455. HTHTETR.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
TIGRFAMsiTIGR03384. betaine_BetI. 1 hit.
PROSITEiView protein in PROSITE
PS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBETI_ECOLI
AccessioniPrimary (citable) accession number: P17446
Secondary accession number(s): Q2MCB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 7, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.