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Protein

HTH-type transcriptional regulator BetI

Gene

betI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Repressor involved in the biosynthesis of the osmoprotectant glycine betaine. It represses transcription of the choline transporter BetT and the genes of BetAB involved in the synthesis of glycine betaine.2 Publications

Miscellaneous

BetI is an unusual repressor because it remains bound to DNA during induction.1 Publication

Pathwayi: betaine biosynthesis via choline pathway

This protein regulates the pathway betaine biosynthesis via choline pathway, which is part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi31 – 50H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • response to osmotic stress Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00251
UniPathwayiUPA00529

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator BetIUniRule annotation
Gene namesi
Name:betIUniRule annotation
Ordered Locus Names:b0313, JW0305
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10111 betI

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

In cells lacking this gene, expression of the bet promoters is stimulated by osmotic stress but not by choline.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000705801 – 195HTH-type transcriptional regulator BetIAdd BLAST195

Proteomic databases

PaxDbiP17446
PRIDEiP17446

Expressioni

Inductioni

Negatively autoregulated.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262808, 6 interactors
IntActiP17446, 10 interactors
STRINGi316385.ECDH10B_0300

Structurei

3D structure databases

ProteinModelPortaliP17446
SMRiP17446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 68HTH tetR-typeUniRule annotationAdd BLAST61

Phylogenomic databases

eggNOGiENOG4108MHR Bacteria
COG1309 LUCA
HOGENOMiHOG000248227
InParanoidiP17446
KOiK02167
OMAiEMEVWFA
PhylomeDBiP17446

Family and domain databases

HAMAPiMF_00768 HTH_type_BetI, 1 hit
InterProiView protein in InterPro
IPR023772 DNA-bd_HTH_TetR-type_CS
IPR009057 Homeobox-like_sf
IPR001647 HTH_TetR
IPR036271 Tet_transcr_reg_TetR-rel_C_sf
IPR017757 Tscrpt_rep_BetI
PfamiView protein in Pfam
PF00440 TetR_N, 1 hit
PRINTSiPR00455 HTHTETR
SUPFAMiSSF46689 SSF46689, 1 hit
SSF48498 SSF48498, 1 hit
TIGRFAMsiTIGR03384 betaine_BetI, 1 hit
PROSITEiView protein in PROSITE
PS01081 HTH_TETR_1, 1 hit
PS50977 HTH_TETR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P17446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKLGMQSIR RRQLIDATLE AINEVGMHDA TIAQIARRAG VSTGIISHYF
60 70 80 90 100
RDKNGLLEAT MRDITSQLRD AVLNRLHALP QGSAEQRLQA IVGGNFDETQ
110 120 130 140 150
VSSAAMKAWL AFWASSMHQP MLYRLQQVSS RRLLSNLVSE FRRELPREQA
160 170 180 190
QEAGYGLAAL IDGLWLRAAL SGKPLDKTRA NSLTRHFITQ HLPTD
Length:195
Mass (Da):21,815
Last modified:August 1, 1990 - v1
Checksum:iB52A77B3A605E354
GO

Sequence cautioni

The sequence AAB18039 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52905 Genomic DNA Translation: CAA37091.1
U73857 Genomic DNA Translation: AAB18039.1 Different initiation.
U00096 Genomic DNA Translation: AAC73416.1
AP009048 Genomic DNA Translation: BAE76096.1
PIRiS15180 S10899
RefSeqiNP_414847.3, NC_000913.3
WP_001335745.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73416; AAC73416; b0313
BAE76096; BAE76096; BAE76096
GeneIDi944981
KEGGiecj:JW0305
eco:b0313
PATRICifig|1411691.4.peg.1964

Similar proteinsi

Entry informationi

Entry nameiBETI_ECOLI
AccessioniPrimary (citable) accession number: P17446
Secondary accession number(s): Q2MCB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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