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P17445

- BETB_ECOLI

UniProt

P17445 - BETB_ECOLI

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Protein
NAD/NADP-dependent betaine aldehyde dehydrogenase
Gene
betB, b0312, JW0304
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid. It is highly specific for betaine and has a significantly higher affinity for NAD than for NADP.2 Publications

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.2 Publications

Cofactori

Binds 2 potassium ions per subunit By similarity.UniRule annotation

Enzyme regulationi

Aldehydes with apolar groups are the most effective inhibitors. N-Methylated compounds are also inhibitory, but to a much lesser degree than the aldehydes. The alcohols are more inhibitory than the corresponding acids, and the inhibitory effect increases with the degree of methylation from ethanolamine to choline.1 Publication

Kineticsi

  1. KM=99 µM for NAD (at pH 7.5 and 25 degrees Celsius)2 Publications
  2. KM=160 µM for betaine aldehyde (at pH 7.5 and 25 degrees Celsius)
  3. KM=400 µM for NADP (at pH 7.5 and 25 degrees Celsius)

Vmax=66 µmol/min/mg enzyme (at pH 7.5 and 25 degrees Celsius)

pH dependencei

Optimum pH is between 7.5 and 9.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261Potassium 1 By similarity
Metal bindingi27 – 271Potassium 1; via carbonyl oxygen By similarity
Metal bindingi93 – 931Potassium 1 By similarity
Active sitei162 – 1621Charge relay system By similarity
Metal bindingi180 – 1801Potassium 1; via carbonyl oxygen By similarity
Binding sitei209 – 2091NAD/NADP; via amide nitrogen By similarity
Metal bindingi246 – 2461Potassium 2; via carbonyl oxygen By similarity
Sitei248 – 2481Seems to be a necessary countercharge to the potassium cations By similarity
Active sitei252 – 2521Proton acceptor By similarity
Binding sitei286 – 2861NAD/NADP By similarity
Binding sitei387 – 3871NAD/NADP By similarity
Metal bindingi457 – 4571Potassium 2; via carbonyl oxygen By similarity
Metal bindingi460 – 4601Potassium 2; via carbonyl oxygen By similarity
Active sitei464 – 4641Charge relay system By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – 1534NAD/NADP By similarity
Nucleotide bindingi176 – 1794NAD/NADP By similarity
Nucleotide bindingi229 – 2346NAD/NADP By similarity

GO - Molecular functioni

  1. betaine-aldehyde dehydrogenase activity Source: EcoCyc
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glycine betaine biosynthetic process from choline Source: EcoCyc
  2. response to osmotic stress Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Metal-binding, NAD, NADP, Potassium

Enzyme and pathway databases

BioCyciEcoCyc:BADH-MONOMER.
ECOL316407:JW0304-MONOMER.
MetaCyc:BADH-MONOMER.
UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenase (EC:1.2.1.8)
Short name:
BADH
Gene namesi
Name:betB
Ordered Locus Names:b0312, JW0304
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10110. betB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 490489NAD/NADP-dependent betaine aldehyde dehydrogenaseUniRule annotation
PRO_0000056541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei286 – 2861Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Proteomic databases

PaxDbiP17445.
PRIDEiP17445.

Expressioni

Inductioni

By osmotic stress. Choline is required for full expression. Oxygen and choline exert their control via the transacting DNA-binding proteins ArcA and BetI, respectively.3 Publications

Gene expression databases

GenevestigatoriP17445.

Interactioni

Subunit structurei

Dimer of dimers Inferred.1 Publication

Protein-protein interaction databases

DIPiDIP-9208N.
IntActiP17445. 7 interactions.
STRINGi511145.b0312.

Structurei

3D structure databases

ProteinModelPortaliP17445.
SMRiP17445. Positions 2-490.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiCTDEMTI.
OrthoDBiEOG6BS8QW.
PhylomeDBiP17445.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17445-1 [UniParc]FASTAAdd to Basket

« Hide

MSRMAEQQLY IHGGYTSATS GRTFETINPA NGNVLATVQA AGREDVDRAV    50
KSAQQGQKIW ASMTAMERSR ILRRAVDILR ERNDELAKLE TLDTGKAYSE 100
TSTVDIVTGA DVLEYYAGLI PALEGSQIPL RETSFVYTRR EPLGVVAGIG 150
AWNYPIQIAL WKSAPALAAG NAMIFKPSEV TPLTALKLAE IYSEAGLPDG 200
VFNVLPGVGA ETGQYLTEHP GIAKVSFTGG VASGKKVMAN SAASSLKEVT 250
MELGGKSPLI VFDDADLDLA ADIAMMANFF SSGQVCTNGT RVFVPAKCKA 300
AFEQKILARV ERIRAGDVFD PQTNFGPLVS FPHRDNVLRY IAKGKEEGAR 350
VLCGGDVLKG DGFDNGAWVA PTVFTDCSDD MTIVREEIFG PVMSILTYES 400
EDEVIRRAND TDYGLAAGIV TADLNRAHRV IHQLEAGICW INTWGESPAE 450
MPVGGYKHSG IGRENGVMTL QSYTQVKSIQ VEMAKFQSIF 490
Length:490
Mass (Da):52,911
Last modified:January 23, 2007 - v3
Checksum:i747F822DA1233808
GO

Sequence cautioni

The sequence AAA23505.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAB18038.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti232 – 2321A → R in AAA23506. 1 Publication
Sequence conflicti232 – 2321A → R in AAA23505. 1 Publication
Sequence conflicti312 – 3121R → P in AAA23506. 1 Publication
Sequence conflicti312 – 3121R → P in AAA23505. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52905 Genomic DNA. Translation: CAA37092.1.
M77739 Genomic DNA. Translation: AAA23506.1.
M77739 Genomic DNA. Translation: AAA23505.1. Different initiation.
U73857 Genomic DNA. Translation: AAB18038.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73415.1.
AP009048 Genomic DNA. Translation: BAE76095.1.
PIRiS15181.
RefSeqiNP_414846.1. NC_000913.3.
YP_488607.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73415; AAC73415; b0312.
BAE76095; BAE76095; BAE76095.
GeneIDi12933807.
947376.
KEGGiecj:Y75_p0302.
eco:b0312.
PATRICi32115753. VBIEscCol129921_0319.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52905 Genomic DNA. Translation: CAA37092.1 .
M77739 Genomic DNA. Translation: AAA23506.1 .
M77739 Genomic DNA. Translation: AAA23505.1 . Different initiation.
U73857 Genomic DNA. Translation: AAB18038.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC73415.1 .
AP009048 Genomic DNA. Translation: BAE76095.1 .
PIRi S15181.
RefSeqi NP_414846.1. NC_000913.3.
YP_488607.1. NC_007779.1.

3D structure databases

ProteinModelPortali P17445.
SMRi P17445. Positions 2-490.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-9208N.
IntActi P17445. 7 interactions.
STRINGi 511145.b0312.

Proteomic databases

PaxDbi P17445.
PRIDEi P17445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73415 ; AAC73415 ; b0312 .
BAE76095 ; BAE76095 ; BAE76095 .
GeneIDi 12933807.
947376.
KEGGi ecj:Y75_p0302.
eco:b0312.
PATRICi 32115753. VBIEscCol129921_0319.

Organism-specific databases

EchoBASEi EB0108.
EcoGenei EG10110. betB.

Phylogenomic databases

eggNOGi COG1012.
HOGENOMi HOG000271505.
KOi K00130.
OMAi CTDEMTI.
OrthoDBi EOG6BS8QW.
PhylomeDBi P17445.

Enzyme and pathway databases

UniPathwayi UPA00529 ; UER00386 .
BioCyci EcoCyc:BADH-MONOMER.
ECOL316407:JW0304-MONOMER.
MetaCyc:BADH-MONOMER.

Miscellaneous databases

PROi P17445.

Gene expression databases

Genevestigatori P17445.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPi MF_00804. BADH.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view ]
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01804. BADH. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli."
    Lamark T., Kaasen E., Eshoo M.W., Falkenberg P., McDougall J., Strom A.R.
    Mol. Microbiol. 5:1049-1064(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-29.
    Strain: K12.
  2. "Characterization of an Escherichia coli gene encoding betaine aldehyde dehydrogenase (BADH): structural similarity to mammalian ALDHs and a plant BADH."
    Boyd L.A., Adam L., Pelcher L.E., McHughen A., Hirji R., Selvaraj G.
    Gene 103:45-52(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli."
    Landfald B., Strom A.R.
    J. Bacteriol. 165:849-855(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
  7. "Purification and characterization of osmoregulatory betaine aldehyde dehydrogenase of Escherichia coli."
    Falkenberg P., Strom A.R.
    Biochim. Biophys. Acta 1034:253-259(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBSTRATE SPECIFICITY, SUBUNIT.
  8. "The complex bet promoters of Escherichia coli: regulation by oxygen (ArcA), choline (BetI), and osmotic stress."
    Lamark T., Rokenes T.P., McDougall J., Strom A.R.
    J. Bacteriol. 178:1655-1662(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiBETB_ECOLI
AccessioniPrimary (citable) accession number: P17445
Secondary accession number(s): Q2MCB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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