Reviewed, UniProtKB/Swiss-Prot P17445 (BETB_ECOLI)

Names and origin

Protein names

Recommended name:
    Betaine aldehyde dehydrogenase
      Short name=BADH
    EC=1.2.1.8

Gene names

Name:	betB
Ordered Locus Names:	b0312, JW0304

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Betaine aldehyde + NAD+ + H2O = betaine + NADH. HAMAP MF_00804
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine from betaine aldehyde: step 1/1. HAMAP MF_00804
Subunit structure	Homotetramer. HAMAP MF_00804
Induction	By osmotic stress. Choline is required for full expression. HAMAP MF_00804
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Stress response
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	betaine-aldehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 490	489	Betaine aldehyde dehydrogenase HAMAP MF_00804		PRO_0000056541
Regions
	Nucleotide binding	229 – 234	6	NAD By similarity
Sites
	Active site	252	1	By similarity
	Active site	286	1	By similarity
Experimental info
	Sequence conflict	232	1	A → R in AAA23506. Ref.2
	Sequence conflict	232	1	A → R in AAA23505. Ref.2
	Sequence conflict	312	1	R → P in AAA23506. Ref.2
	Sequence conflict	312	1	R → P in AAA23505. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P17445-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 747F822DA1233808	FASTA	490	52,911
	10 20 30 40 50 60 MSRMAEQQLY IHGGYTSATS GRTFETINPA NGNVLATVQA AGREDVDRAV KSAQQGQKIW 70 80 90 100 110 120 ASMTAMERSR ILRRAVDILR ERNDELAKLE TLDTGKAYSE TSTVDIVTGA DVLEYYAGLI 130 140 150 160 170 180 PALEGSQIPL RETSFVYTRR EPLGVVAGIG AWNYPIQIAL WKSAPALAAG NAMIFKPSEV 190 200 210 220 230 240 TPLTALKLAE IYSEAGLPDG VFNVLPGVGA ETGQYLTEHP GIAKVSFTGG VASGKKVMAN 250 260 270 280 290 300 SAASSLKEVT MELGGKSPLI VFDDADLDLA ADIAMMANFF SSGQVCTNGT RVFVPAKCKA 310 320 330 340 350 360 AFEQKILARV ERIRAGDVFD PQTNFGPLVS FPHRDNVLRY IAKGKEEGAR VLCGGDVLKG 370 380 390 400 410 420 DGFDNGAWVA PTVFTDCSDD MTIVREEIFG PVMSILTYES EDEVIRRAND TDYGLAAGIV 430 440 450 460 470 480 TADLNRAHRV IHQLEAGICW INTWGESPAE MPVGGYKHSG IGRENGVMTL QSYTQVKSIQ 490 VEMAKFQSIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli." Lamark T., Kaasen E., Eshoo M.W., Falkenberg P., McDougall J., Strom A.R. Mol. Microbiol. 5:1049-1064(1991) [PubMed: 1956285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-29. Strain: K12.
[2]	"Characterization of an Escherichia coli gene encoding betaine aldehyde dehydrogenase (BADH): structural similarity to mammalian ALDHs and a plant BADH." Boyd L.A., Adam L., Pelcher L.E., McHughen A., Hirji R., Selvaraj G. Gene 103:45-52(1991) [PubMed: 1879697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52905 Genomic DNA. Translation: CAA37092.1. M77739 Genomic DNA. Translation: AAA23506.1. M77739 Genomic DNA. Translation: AAA23505.1. Different initiation. U73857 Genomic DNA. Translation: AAB18038.1. Different initiation. U00096 Genomic DNA. Translation: AAC73415.1. AP009048 Genomic DNA. Translation: BAE76095.1.
PIR	S15181.
RefSeq	AP_000965.1. NP_414846.1.
3D structure databases
HSSP	HSSP built from PDB template 1A4S based on UniProtKB P56533.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9208N.
Genome annotation databases
GeneID	947376.
GenomeReviews	Gene locus JW0304 in contig AP009048_GR. Gene locus b0312 in contig U00096_GR.
KEGG	ecj:JW0304. eco:b0312.
Organism-specific databases
EchoBASE	EB0108.
EcoGene	EG10110. betB.
CMR	Search...
Phylogenomic databases
HOGENOM	P17445.
OMA	P17445. TLKEVTM.
Enzyme and pathway databases
BioCyc	EcoCyc:BADH-MON. MetaCyc:BADH-MON.
Family and domain databases
HAMAP	MF_00804. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR011264. Betaine_Ald_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01804. BADH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETB_ECOLI

Accession

Primary (citable) accession number: P17445
Secondary accession number(s): Q2MCB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)