P17444 (BETA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Choline dehydrogenase
Short name=CDH
Short name=CHD
EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	b0311, JW0303

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine at the same rate. HAMAP-Rule MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP-Rule MF_00750
Cofactor	FAD.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP-Rule MF_00750
Subcellular location	Cell membrane; Peripheral membrane protein HAMAP-Rule MF_00750.
Induction	By osmotic stress. Choline is required for full expression. HAMAP-Rule MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.
Sequence caution	The sequence AAB18037.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycine betaine biosynthetic process from choline Inferred from mutant phenotype PubMed 3512526. Source: EcoCyc response to osmotic stress Inferred from mutant phenotype PubMed 3512526. Source: EcoCyc
Cellular_component	membrane Inferred from direct assay PubMed 3512525. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	choline dehydrogenase activity Inferred from direct assay PubMed 3512525. Source: EcoCyc flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
surE	P0A840	1	EBI-1115667,EBI-1126761

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Choline dehydrogenase HAMAP-Rule MF_00750		PRO_0000205586
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	473	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17444 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: E182480168C8E9FD	FASTA	556	61,878
	10 20 30 40 50 60 MQFDYIIIGA GSAGNVLATR LTEDPNTSVL LLEAGGPDYR FDFRTQMPAA LAFPLQGKRY 70 80 90 100 110 120 NWAYETEPEP FMNNRRMECG RGKGLGGSSL INGMCYIRGN ALDLDNWAQE PGLENWSYLD 130 140 150 160 170 180 CLPYYRKAET RDMGENDYHG GDGPVSVTTS KPGVNPLFEA MIEAGVQAGY PRTDDLNGYQ 190 200 210 220 230 240 QEGFGPMDRT VTPQGRRAST ARGYLDQAKS RPNLTIRTHA MTDHIIFDGK RAVGVEWLEG 250 260 270 280 290 300 DSTIPTRATA NKEVLLCAGA IASPQILQRS GVGNAELLAE FDIPLVHELP GVGENLQDHL 310 320 330 340 350 360 EMYLQYECKE PVSLYPALQW WNQPKIGAEW LFGGTGVGAS NHFEAGGFIR SREEFAWPNI 370 380 390 400 410 420 QYHFLPVAIN YNGSNAVKEH GFQCHVGSMR SPSRGHVRIK SRDPHQHPAI LFNYMSHEQD 430 440 450 460 470 480 WQEFRDAIRI TREIMHQPAL DQYRGREISP GVECQTDEQL DEFVRNHAET AFHPCGTCKM 490 500 510 520 530 540 GYDEMSVVDG EGRVHGLEGL RVVDASIMPQ IITGNLNATT IMIGEKIADM IRGQEALPRS 550 TAGYFVANGM PVRAKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli." Lamark T., Kaasen E., Eshoo M.W., Falkenberg P., McDougall J., Strom A.R. Mol. Microbiol. 5:1049-1064(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Characterization of an Escherichia coli gene encoding betaine aldehyde dehydrogenase (BADH): structural similarity to mammalian ALDHs and a plant BADH." Boyd L.A., Adam L., Pelcher L.E., McHughen A., Hirji R., Selvaraj G. Gene 103:45-52(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52905 Genomic DNA. Translation: CAA37093.1. M77738 Genomic DNA. Translation: AAA23504.1. U73857 Genomic DNA. Translation: AAB18037.1. Different initiation. U00096 Genomic DNA. Translation: AAC73414.1. AP009048 Genomic DNA. Translation: BAE76094.1.
PIR	S10901. S15182.
RefSeq	NP_414845.1. NC_000913.2. YP_488606.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P17444.
SMR	P17444. Positions 2-532.
ModBase	Search...
Protein-protein interaction databases
IntAct	P17444. 7 interactions.
STRING	511145.b0311.
Proteomic databases
PRIDE	P17444.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73414; AAC73414; b0311. BAE76094; BAE76094; BAE76094.
GeneID	12933806. 945716.
KEGG	ecj:Y75_p0301. eco:b0311.
PATRIC	32115751. VBIEscCol129921_0318.
Organism-specific databases
EchoBASE	EB0107.
EcoGene	EG10109. betA.
Phylogenomic databases
eggNOG	COG2303.
HOGENOM	HOG000139600.
KO	K00108.
OMA	AMDYDGW.
ProtClustDB	PRK02106.
Enzyme and pathway databases
BioCyc	EcoCyc:CHD-MONOMER. ECOL316407:JW0303-MONOMER. MetaCyc:CHD-MONOMER.
UniPathway	UPA00529; UER00385.
Gene expression databases
Genevestigator	P17444.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen.
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_ECOLI

Accession

Primary (citable) accession number: P17444
Secondary accession number(s): P77861, Q2MCB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program