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Protein

UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

Gene

murG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).

Catalytic activityi

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: UniProtKB-KW
  • lipid glycosylation Source: UniProtKB-HAMAP
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:NACGLCTRANS-MONOMER.
ECOL316407:JW0088-MONOMER.
MetaCyc:NACGLCTRANS-MONOMER.
BRENDAi2.4.1.227. 2026.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferaseUniRule annotation (EC:2.4.1.227UniRule annotation)
Alternative name(s):
Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferaseUniRule annotation
Gene namesi
Name:murGUniRule annotation
Ordered Locus Names:b0090, JW0088
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10623. murG.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Peripheral membrane protein UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 355354UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferasePRO_0000109170Add
BLAST

Proteomic databases

PaxDbiP17443.
PRIDEiP17443.

Interactioni

Protein-protein interaction databases

BioGridi4261859. 551 interactions.
DIPiDIP-10282N.
IntActiP17443. 26 interactions.
MINTiMINT-1233082.
STRINGi511145.b0090.

Structurei

Secondary structure

1
355
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115Combined sources
Helixi16 – 3015Combined sources
Turni31 – 333Combined sources
Beta strandi35 – 406Combined sources
Helixi46 – 494Combined sources
Helixi50 – 534Combined sources
Beta strandi56 – 594Combined sources
Helixi63 – 653Combined sources
Helixi70 – 745Combined sources
Helixi77 – 9418Combined sources
Beta strandi97 – 1015Combined sources
Helixi107 – 11610Combined sources
Beta strandi121 – 1255Combined sources
Beta strandi127 – 1293Combined sources
Helixi132 – 1376Combined sources
Turni138 – 1403Combined sources
Beta strandi142 – 1487Combined sources
Beta strandi151 – 1544Combined sources
Helixi164 – 1674Combined sources
Helixi172 – 1765Combined sources
Beta strandi181 – 1888Combined sources
Turni190 – 1923Combined sources
Helixi195 – 20814Combined sources
Helixi209 – 2113Combined sources
Beta strandi212 – 2176Combined sources
Helixi223 – 23210Combined sources
Beta strandi238 – 2425Combined sources
Helixi247 – 2537Combined sources
Beta strandi255 – 2595Combined sources
Helixi263 – 27210Combined sources
Beta strandi276 – 2783Combined sources
Helixi287 – 29711Combined sources
Beta strandi300 – 3034Combined sources
Helixi306 – 3083Combined sources
Helixi311 – 3199Combined sources
Helixi323 – 33513Combined sources
Helixi341 – 35313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F0KX-ray1.90A/B1-355[»]
1NLMX-ray2.50A/B2-355[»]
ProteinModelPortaliP17443.
SMRiP17443. Positions 6-355.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17443.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family. MurG subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DVQ. Bacteria.
COG0707. LUCA.
HOGENOMiHOG000218321.
InParanoidiP17443.
KOiK02563.
OMAiHQTKNAM.
PhylomeDBiP17443.

Family and domain databases

HAMAPiMF_00033. MurG. 1 hit.
InterProiIPR006009. GlcNAc_MurG.
IPR007235. Glyco_trans_28_C.
IPR004276. GlycoTrans_28_N.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF03033. Glyco_transf_28. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01133. murG. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQGKRLMV MAGGTGGHVF PGLAVAHHLM AQGWQVRWLG TADRMEADLV
60 70 80 90 100
PKHGIEIDFI RISGLRGKGI KALIAAPLRI FNAWRQARAI MKAYKPDVVL
110 120 130 140 150
GMGGYVSGPG GLAAWSLGIP VVLHEQNGIA GLTNKWLAKI ATKVMQAFPG
160 170 180 190 200
AFPNAEVVGN PVRTDVLALP LPQQRLAGRE GPVRVLVVGG SQGARILNQT
210 220 230 240 250
MPQVAAKLGD SVTIWHQSGK GSQQSVEQAY AEAGQPQHKV TEFIDDMAAA
260 270 280 290 300
YAWADVVVCR SGALTVSEIA AAGLPALFVP FQHKDRQQYW NALPLEKAGA
310 320 330 340 350
AKIIEQPQLS VDAVANTLAG WSRETLLTMA ERARAASIPD ATERVANEVS

RVARA
Length:355
Mass (Da):37,815
Last modified:January 23, 2007 - v3
Checksum:iDE347361A7B9293A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA. Translation: CAA36867.1.
X52540 Genomic DNA. Translation: CAA36776.1.
X55034 Genomic DNA. Translation: CAA38867.1.
U00096 Genomic DNA. Translation: AAC73201.1.
AP009048 Genomic DNA. Translation: BAB96658.1.
PIRiJQ0544. BVECMG.
RefSeqiNP_414632.1. NC_000913.3.
WP_000016560.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73201; AAC73201; b0090.
BAB96658; BAB96658; BAB96658.
GeneIDi946321.
KEGGiecj:JW0088.
eco:b0090.
PATRICi32115285. VBIEscCol129921_0094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA. Translation: CAA36867.1.
X52540 Genomic DNA. Translation: CAA36776.1.
X55034 Genomic DNA. Translation: CAA38867.1.
U00096 Genomic DNA. Translation: AAC73201.1.
AP009048 Genomic DNA. Translation: BAB96658.1.
PIRiJQ0544. BVECMG.
RefSeqiNP_414632.1. NC_000913.3.
WP_000016560.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F0KX-ray1.90A/B1-355[»]
1NLMX-ray2.50A/B2-355[»]
ProteinModelPortaliP17443.
SMRiP17443. Positions 6-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261859. 551 interactions.
DIPiDIP-10282N.
IntActiP17443. 26 interactions.
MINTiMINT-1233082.
STRINGi511145.b0090.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Proteomic databases

PaxDbiP17443.
PRIDEiP17443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73201; AAC73201; b0090.
BAB96658; BAB96658; BAB96658.
GeneIDi946321.
KEGGiecj:JW0088.
eco:b0090.
PATRICi32115285. VBIEscCol129921_0094.

Organism-specific databases

EchoBASEiEB0618.
EcoGeneiEG10623. murG.

Phylogenomic databases

eggNOGiENOG4105DVQ. Bacteria.
COG0707. LUCA.
HOGENOMiHOG000218321.
InParanoidiP17443.
KOiK02563.
OMAiHQTKNAM.
PhylomeDBiP17443.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:NACGLCTRANS-MONOMER.
ECOL316407:JW0088-MONOMER.
MetaCyc:NACGLCTRANS-MONOMER.
BRENDAi2.4.1.227. 2026.

Miscellaneous databases

EvolutionaryTraceiP17443.
PROiP17443.

Family and domain databases

HAMAPiMF_00033. MurG. 1 hit.
InterProiIPR006009. GlcNAc_MurG.
IPR007235. Glyco_trans_28_C.
IPR004276. GlycoTrans_28_N.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF03033. Glyco_transf_28. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01133. murG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURG_ECOLI
AccessioniPrimary (citable) accession number: P17443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.