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Protein

UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

Gene

murG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).

Catalytic activityi

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:NACGLCTRANS-MONOMER.
ECOL316407:JW0088-MONOMER.
MetaCyc:NACGLCTRANS-MONOMER.
BRENDAi2.4.1.227. 2026.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferaseUniRule annotation (EC:2.4.1.227UniRule annotation)
Alternative name(s):
Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferaseUniRule annotation
Gene namesi
Name:murGUniRule annotation
Ordered Locus Names:b0090, JW0088
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10623. murG.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Peripheral membrane protein UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3596078.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001091702 – 355UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferaseAdd BLAST354

Proteomic databases

PaxDbiP17443.
PRIDEiP17443.

Interactioni

Protein-protein interaction databases

BioGridi4261859. 551 interactors.
DIPiDIP-10282N.
IntActiP17443. 26 interactors.
MINTiMINT-1233082.
STRINGi511145.b0090.

Structurei

Secondary structure

1355
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi16 – 30Combined sources15
Turni31 – 33Combined sources3
Beta strandi35 – 40Combined sources6
Helixi46 – 49Combined sources4
Helixi50 – 53Combined sources4
Beta strandi56 – 59Combined sources4
Helixi63 – 65Combined sources3
Helixi70 – 74Combined sources5
Helixi77 – 94Combined sources18
Beta strandi97 – 101Combined sources5
Helixi107 – 116Combined sources10
Beta strandi121 – 125Combined sources5
Beta strandi127 – 129Combined sources3
Helixi132 – 137Combined sources6
Turni138 – 140Combined sources3
Beta strandi142 – 148Combined sources7
Beta strandi151 – 154Combined sources4
Helixi164 – 167Combined sources4
Helixi172 – 176Combined sources5
Beta strandi181 – 188Combined sources8
Turni190 – 192Combined sources3
Helixi195 – 208Combined sources14
Helixi209 – 211Combined sources3
Beta strandi212 – 217Combined sources6
Helixi223 – 232Combined sources10
Beta strandi238 – 242Combined sources5
Helixi247 – 253Combined sources7
Beta strandi255 – 259Combined sources5
Helixi263 – 272Combined sources10
Beta strandi276 – 278Combined sources3
Helixi287 – 297Combined sources11
Beta strandi300 – 303Combined sources4
Helixi306 – 308Combined sources3
Helixi311 – 319Combined sources9
Helixi323 – 335Combined sources13
Helixi341 – 353Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F0KX-ray1.90A/B1-355[»]
1NLMX-ray2.50A/B2-355[»]
ProteinModelPortaliP17443.
SMRiP17443.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17443.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family. MurG subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DVQ. Bacteria.
COG0707. LUCA.
HOGENOMiHOG000218321.
InParanoidiP17443.
KOiK02563.
OMAiHQTKNAM.
PhylomeDBiP17443.

Family and domain databases

CDDicd03785. GT1_MurG. 1 hit.
HAMAPiMF_00033. MurG. 1 hit.
InterProiIPR006009. GlcNAc_MurG.
IPR007235. Glyco_trans_28_C.
IPR004276. GlycoTrans_28_N.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF03033. Glyco_transf_28. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01133. murG. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQGKRLMV MAGGTGGHVF PGLAVAHHLM AQGWQVRWLG TADRMEADLV
60 70 80 90 100
PKHGIEIDFI RISGLRGKGI KALIAAPLRI FNAWRQARAI MKAYKPDVVL
110 120 130 140 150
GMGGYVSGPG GLAAWSLGIP VVLHEQNGIA GLTNKWLAKI ATKVMQAFPG
160 170 180 190 200
AFPNAEVVGN PVRTDVLALP LPQQRLAGRE GPVRVLVVGG SQGARILNQT
210 220 230 240 250
MPQVAAKLGD SVTIWHQSGK GSQQSVEQAY AEAGQPQHKV TEFIDDMAAA
260 270 280 290 300
YAWADVVVCR SGALTVSEIA AAGLPALFVP FQHKDRQQYW NALPLEKAGA
310 320 330 340 350
AKIIEQPQLS VDAVANTLAG WSRETLLTMA ERARAASIPD ATERVANEVS

RVARA
Length:355
Mass (Da):37,815
Last modified:January 23, 2007 - v3
Checksum:iDE347361A7B9293A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA. Translation: CAA36867.1.
X52540 Genomic DNA. Translation: CAA36776.1.
X55034 Genomic DNA. Translation: CAA38867.1.
U00096 Genomic DNA. Translation: AAC73201.1.
AP009048 Genomic DNA. Translation: BAB96658.1.
PIRiJQ0544. BVECMG.
RefSeqiNP_414632.1. NC_000913.3.
WP_000016560.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73201; AAC73201; b0090.
BAB96658; BAB96658; BAB96658.
GeneIDi946321.
KEGGiecj:JW0088.
eco:b0090.
PATRICi32115285. VBIEscCol129921_0094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52644 Genomic DNA. Translation: CAA36867.1.
X52540 Genomic DNA. Translation: CAA36776.1.
X55034 Genomic DNA. Translation: CAA38867.1.
U00096 Genomic DNA. Translation: AAC73201.1.
AP009048 Genomic DNA. Translation: BAB96658.1.
PIRiJQ0544. BVECMG.
RefSeqiNP_414632.1. NC_000913.3.
WP_000016560.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F0KX-ray1.90A/B1-355[»]
1NLMX-ray2.50A/B2-355[»]
ProteinModelPortaliP17443.
SMRiP17443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261859. 551 interactors.
DIPiDIP-10282N.
IntActiP17443. 26 interactors.
MINTiMINT-1233082.
STRINGi511145.b0090.

Chemistry databases

ChEMBLiCHEMBL3596078.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Proteomic databases

PaxDbiP17443.
PRIDEiP17443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73201; AAC73201; b0090.
BAB96658; BAB96658; BAB96658.
GeneIDi946321.
KEGGiecj:JW0088.
eco:b0090.
PATRICi32115285. VBIEscCol129921_0094.

Organism-specific databases

EchoBASEiEB0618.
EcoGeneiEG10623. murG.

Phylogenomic databases

eggNOGiENOG4105DVQ. Bacteria.
COG0707. LUCA.
HOGENOMiHOG000218321.
InParanoidiP17443.
KOiK02563.
OMAiHQTKNAM.
PhylomeDBiP17443.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:NACGLCTRANS-MONOMER.
ECOL316407:JW0088-MONOMER.
MetaCyc:NACGLCTRANS-MONOMER.
BRENDAi2.4.1.227. 2026.

Miscellaneous databases

EvolutionaryTraceiP17443.
PROiP17443.

Family and domain databases

CDDicd03785. GT1_MurG. 1 hit.
HAMAPiMF_00033. MurG. 1 hit.
InterProiIPR006009. GlcNAc_MurG.
IPR007235. Glyco_trans_28_C.
IPR004276. GlycoTrans_28_N.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF03033. Glyco_transf_28. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01133. murG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURG_ECOLI
AccessioniPrimary (citable) accession number: P17443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.