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Protein

Phosphate system positive regulatory protein PHO81

Gene

PHO81

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits the kinase activity of the cyclin-CDKs PHO80-PHO85 and PCL7-PHO85 under low-phosphate conditions.3 Publications

Miscellaneous

Present with 2930 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase inhibitor activity Source: SGD
  • phosphoric diester hydrolase activity Source: InterPro

GO - Biological processi

  • lipid metabolic process Source: InterPro
  • phosphate-containing compound metabolic process Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-30911-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate system positive regulatory protein PHO81
Alternative name(s):
CDK inhibitor PHO81
Gene namesi
Name:PHO81
Ordered Locus Names:YGR233C
ORF Names:G8567
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR233C
SGDiS000003465 PHO81

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1250353

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670751 – 1178Phosphate system positive regulatory protein PHO81Add BLAST1178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei956PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the cyclin-CDK PHO80-PHO85. Phosphorylation mediates the formation of a stable interaction with the cyclin-CDK and is required for function as an active inhibitor of the complex under phosphate starvation conditions.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP17442
PaxDbiP17442
PRIDEiP17442

PTM databases

iPTMnetiP17442

Interactioni

Subunit structurei

Associates specifically with the PHO80-PHO85 and PCL7-PHO85 cyclin-CDK complexes, and much of this interaction is mediated through the PHO80 and PCL7 cyclin subunits. Interacts with the transcription factor PHO4.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO85P171576EBI-13314,EBI-13327

Protein-protein interaction databases

BioGridi33485, 63 interactors
DIPiDIP-5959N
IntActiP17442, 17 interactors
MINTiP17442
STRINGi4932.YGR233C

Chemistry databases

BindingDBiP17442

Structurei

3D structure databases

ProteinModelPortaliP17442
SMRiP17442
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 169SPXPROSITE-ProRule annotationAdd BLAST169
Repeati423 – 452ANK 1Add BLAST30
Repeati458 – 487ANK 2Add BLAST30
Repeati506 – 535ANK 3Add BLAST30
Repeati556 – 586ANK 4Add BLAST31
Repeati591 – 620ANK 5Add BLAST30
Repeati624 – 653ANK 6Add BLAST30
Domaini871 – 1178GP-PDEAdd BLAST308

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi198 – 253Asn/Asp-richAdd BLAST56
Compositional biasi231 – 248Poly-AsnAdd BLAST18

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00880000138051
HOGENOMiHOG000187674
InParanoidiP17442
KOiK06653
OMAiWKQPNFP
OrthoDBiEOG092C3FFX

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR030395 GP_PDE_dom
IPR004331 SPX_dom
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF03105 SPX, 2 hits
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
PS51704 GP_PDE, 1 hit
PS51382 SPX, 1 hit

Sequencei

Sequence statusi: Complete.

P17442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFGKYLEAR QLELAEYNSH FIDYKALKKL IKQLAIPTLK ASSDLDLHLT
60 70 80 90 100
LDDIDEKIIH QRLQENKAAF FFKLERELEK VNGYYLARES DLRIKFNILH
110 120 130 140 150
SKYKDYKING KLNSNQATSF KNLYAAFKKF QKDLRNLEQY VELNKTGFSK
160 170 180 190 200
ALKKWDKRSQ SHDKDFYLAT VVSIQPIFTR DGPLKLNDET LHILLELNDI
210 220 230 240 250
DNNNRRADIQ SSTFTNDDDD DNNTSNNNKH NNNNNNNNNN NNNNNNNNIL
260 270 280 290 300
HNNYELTTSK ISENQLEHLF QASSSSLDME MEIENWYKEI LNIATVKDVQ
310 320 330 340 350
RKHALLRNFR ETKIFTYLLQ NSSESFHKNV FSLLKECLTT LFLLLVASPL
360 370 380 390 400
DDNSLHIFYK SNQDHIDLSY CDEDDQVFSR KNVFHEAASC PEKSRLFILD
410 420 430 440 450
EALTTSKLSK ETVQKLLNAQ DIHSRVPLHY AAELGKLEFV HSLLITNLLE
460 470 480 490 500
DVDPIDSDSK TPLVLAITNN HIDVVRDLLT IGGANASPIE KPILDYSKNV
510 520 530 540 550
ISSTKVQFDP LNVACKFNNH DAAKLLLEIR SKQNADNAKN KSSQHLCQPL
560 570 580 590 600
FKKNSTGLCT LHIVAKIGGD PQLIQLLIRY GADPNEIDGF NKWTPIFYAV
610 620 630 640 650
RSGHSEVITE LLKHNARLDI EDDNGHSPLF YALWESHVDV LNALLQRPLN
660 670 680 690 700
LPSAPLNEIN SQSSTQRLNT IDLTPNDDKF DLDIQDSIPD FALPPPIIPL
710 720 730 740 750
RKYGHNFLEK KIFIKLKLRP GLESIKLTQD NGIIMSSSPG RITLSSNLPE
760 770 780 790 800
IIPRNVILPV RSGEINNFCK DISETNDEED DDEISEDHDD GEIIFQVDSI
810 820 830 840 850
DDFSMDFEIF PSFGTRIIAK TTAMPFLFKK VAINSIATMN LPLFDTRLNN
860 870 880 890 900
IGSLTLDYQI IFPYPGNPLK IINYEPYWKS TGSDLMTSSK DGNFVTSSSL
910 920 930 940 950
NGSFISVLVC ALNDETIVAA PKPYVEFKGT KILLNDLTKE QLEKVVDYDF
960 970 980 990 1000
GKIDGSFDEV TLKQYLSSRV VPLRSLLEVI PGSAQLVIRV YFPTDKEIDT
1010 1020 1030 1040 1050
IPIKISPFIN INQFIDKLLL IIFEHERFLR HSGSGSMRQI VFSSCNWEAC
1060 1070 1080 1090 1100
SILNWKQPNF PVLLQMKNLL RDSTTGKFVG DTPNCLKELA VNPQKMSYLN
1110 1120 1130 1140 1150
TELINIHTMV QFAMNNNLLG VTLPYEVLKI CPSLARIIKQ NGLLLIASVG
1160 1170
ENDQIPADGG YSGIYYACEL LFENNIDM
Length:1,178
Mass (Da):134,029
Last modified:October 1, 1996 - v2
Checksum:i9314EDB94B3F6B7D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248N → NN in BAA02508 (PubMed:8492812).Curated1
Sequence conflicti728T → I in BAA02508 (PubMed:8492812).Curated1
Sequence conflicti762S → F in BAA02508 (PubMed:8492812).Curated1
Sequence conflicti845D → H in BAA02508 (PubMed:8492812).Curated1
Sequence conflicti873N → K in BAA02508 (PubMed:8492812).Curated1
Sequence conflicti920Missing in CAA36726 (PubMed:2186378).Curated1
Sequence conflicti984A → V in BAA02508 (PubMed:8492812).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52482 Genomic DNA Translation: CAA36726.1
D13228 Genomic DNA Translation: BAA02508.1
X87941 Genomic DNA Translation: CAA61183.1
Z73018 Genomic DNA Translation: CAA97261.1
S61041 Genomic DNA Translation: AAD13922.1
BK006941 Genomic DNA Translation: DAA08324.1
PIRiS57698
RefSeqiNP_011749.3, NM_001181362.3

Genome annotation databases

EnsemblFungiiYGR233C; YGR233C; YGR233C
GeneIDi853148
KEGGisce:YGR233C

Similar proteinsi

Entry informationi

Entry nameiPHO81_YEAST
AccessioniPrimary (citable) accession number: P17442
Secondary accession number(s): D6VV13, Q06887
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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