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Protein

Glucosylceramidase

Gene

Gba

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei254Proton donorBy similarity1
Active sitei358NucleophileBy similarity1

GO - Molecular functioni

  • glucosylceramidase activity Source: ParkinsonsUK-UCL
  • hydrolase activity Source: MGI
  • receptor binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi3.2.1.45. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosylceramidase (EC:3.2.1.45)
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene namesi
Name:Gba
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95665. Gba.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • lysosomal lumen Source: BHF-UCL
  • lysosomal membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2278.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000001217820 – 515GlucosylceramidaseAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 35By similarity
Disulfide bondi37 ↔ 42By similarity
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1
Glycosylationi78N-linked (GlcNAc...)Sequence analysis1
Glycosylationi165N-linked (GlcNAc...) (high mannose)1 Publication1
Glycosylationi289N-linked (GlcNAc...)1 Publication1
Glycosylationi480N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP17439.
MaxQBiP17439.
PaxDbiP17439.
PeptideAtlasiP17439.
PRIDEiP17439.

PTM databases

iPTMnetiP17439.
PhosphoSitePlusiP17439.
SwissPalmiP17439.
UniCarbKBiP17439.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028048.
CleanExiMM_GBA.
ExpressionAtlasiP17439. baseline and differential.
GenevisibleiP17439. MM.

Interactioni

Subunit structurei

Interacts with SCARB2.By similarity

GO - Molecular functioni

  • receptor binding Source: BHF-UCL

Protein-protein interaction databases

IntActiP17439. 4 interactors.
MINTiMINT-4096112.
STRINGi10090.ENSMUSP00000076589.

Chemistry databases

BindingDBiP17439.

Structurei

3D structure databases

ProteinModelPortaliP17439.
SMRiP17439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2566. Eukaryota.
COG5520. LUCA.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
HOVERGENiHBG002285.
InParanoidiP17439.
KOiK01201.
OMAiQPDQKFQ.
OrthoDBiEOG091G06I4.
PhylomeDBiP17439.
TreeFamiTF314254.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR033452. GH30_C.
IPR001139. Glyco_hydro_30.
IPR033453. Glyco_hydro_30_TIM-barrel.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
PF17189. Glyco_hydro_30C. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17439-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARLIGFFL FQAVSWAYGA QPCIPKSFGY SSVVCVCNAS YCDSLDPVTL
60 70 80 90 100
PALGTFSRYE STRRGRRMEL SVGAIQANRT GTGLLLTLQP EKKFQKVKGF
110 120 130 140 150
GGAMTDATAL NILALSPPTQ KLLLRSYFST NGIEYNIIRV PMASCDFSIR
160 170 180 190 200
VYTYADTPND FQLSNFSLPE EDTKLKIPLI HQALKMSSRP ISLFASPWTS
210 220 230 240 250
PTWLKTNGRV NGKGSLKGQP GDIFHQTWAN YFVKFLDAYA KYGLRFWAVT
260 270 280 290 300
AENEPTAGLF TGYPFQCLGF TPEHQRDFIS RDLGPALANS SHDVKLLMLD
310 320 330 340 350
DQRLLLPRWA EVVLSDPEAA KYVHGIAVHW YMDFLAPAKA TLGETHRLFP
360 370 380 390 400
NTMLFASEAC VGSKFWEQSV RLGSWDRGMQ YSHSIITNLL YHVTGWTDWN
410 420 430 440 450
LALNPEGGPN WVRNFVDSPI IVDIPKDAFY KQPMFYHLGH FSKFIPEGSQ
460 470 480 490 500
RVALVASEST DLETVALLRP DGSAVVVVLN RSSEDVPLTI SDPDLGFLET
510
VSPGYSIHTY LWRRQ
Length:515
Mass (Da):57,622
Last modified:August 1, 1990 - v1
Checksum:i7CCD9176085FE2CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24119 mRNA. Translation: AAA37671.1.
AY115108 Genomic DNA. Translation: AAM66757.1.
AK082767 mRNA. Translation: BAC38609.1.
BC006663 mRNA. Translation: AAH06663.1.
M89949 Genomic DNA. Translation: AAA37665.1.
CCDSiCCDS17493.1.
PIRiA32931.
RefSeqiNP_001070879.1. NM_001077411.2.
NP_032120.1. NM_008094.5.
UniGeneiMm.5031.

Genome annotation databases

EnsembliENSMUST00000077367; ENSMUSP00000076589; ENSMUSG00000028048.
ENSMUST00000167998; ENSMUSP00000130660; ENSMUSG00000028048.
GeneIDi14466.
KEGGimmu:14466.
UCSCiuc008pyb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24119 mRNA. Translation: AAA37671.1.
AY115108 Genomic DNA. Translation: AAM66757.1.
AK082767 mRNA. Translation: BAC38609.1.
BC006663 mRNA. Translation: AAH06663.1.
M89949 Genomic DNA. Translation: AAA37665.1.
CCDSiCCDS17493.1.
PIRiA32931.
RefSeqiNP_001070879.1. NM_001077411.2.
NP_032120.1. NM_008094.5.
UniGeneiMm.5031.

3D structure databases

ProteinModelPortaliP17439.
SMRiP17439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP17439. 4 interactors.
MINTiMINT-4096112.
STRINGi10090.ENSMUSP00000076589.

Chemistry databases

BindingDBiP17439.
ChEMBLiCHEMBL2278.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

PTM databases

iPTMnetiP17439.
PhosphoSitePlusiP17439.
SwissPalmiP17439.
UniCarbKBiP17439.

Proteomic databases

EPDiP17439.
MaxQBiP17439.
PaxDbiP17439.
PeptideAtlasiP17439.
PRIDEiP17439.

Protocols and materials databases

DNASUi14466.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077367; ENSMUSP00000076589; ENSMUSG00000028048.
ENSMUST00000167998; ENSMUSP00000130660; ENSMUSG00000028048.
GeneIDi14466.
KEGGimmu:14466.
UCSCiuc008pyb.2. mouse.

Organism-specific databases

CTDi2629.
MGIiMGI:95665. Gba.

Phylogenomic databases

eggNOGiKOG2566. Eukaryota.
COG5520. LUCA.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
HOVERGENiHBG002285.
InParanoidiP17439.
KOiK01201.
OMAiQPDQKFQ.
OrthoDBiEOG091G06I4.
PhylomeDBiP17439.
TreeFamiTF314254.

Enzyme and pathway databases

BRENDAi3.2.1.45. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Miscellaneous databases

PROiP17439.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028048.
CleanExiMM_GBA.
ExpressionAtlasiP17439. baseline and differential.
GenevisibleiP17439. MM.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR033452. GH30_C.
IPR001139. Glyco_hydro_30.
IPR033453. Glyco_hydro_30_TIM-barrel.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
PF17189. Glyco_hydro_30C. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLCM_MOUSE
AccessioniPrimary (citable) accession number: P17439
Secondary accession number(s): Q78NR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.