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P17433

- SPI1_MOUSE

UniProt

P17433 - SPI1_MOUSE

Protein

Transcription factor PU.1

Gene

Spi1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 2 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi172 – 25584ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: BHF-UCL
    2. NFAT protein binding Source: BHF-UCL
    3. protein binding Source: IntAct
    4. RNA binding Source: UniProtKB-KW
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    7. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    8. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
    9. RNA polymerase II distal enhancer sequence-specific DNA binding Source: BHF-UCL
    10. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    11. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: BHF-UCL
    12. RNA polymerase II transcription factor binding Source: BHF-UCL
    13. sequence-specific DNA binding Source: MGI
    14. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. anatomical structure regression Source: MGI
    2. apoptotic process involved in patterning of blood vessels Source: MGI
    3. erythrocyte differentiation Source: MGI
    4. granulocyte differentiation Source: MGI
    5. histone H3 acetylation Source: Ensembl
    6. hypermethylation of CpG island Source: Ensembl
    7. lymphocyte differentiation Source: MGI
    8. lymphoid progenitor cell differentiation Source: MGI
    9. macrophage differentiation Source: MGI
    10. myeloid dendritic cell differentiation Source: MGI
    11. myeloid leukocyte differentiation Source: MGI
    12. negative regulation of gene expression, epigenetic Source: Ensembl
    13. negative regulation of histone H4 acetylation Source: Ensembl
    14. negative regulation of MHC class II biosynthetic process Source: BHF-UCL
    15. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    16. positive regulation of transcription, DNA-templated Source: MGI
    17. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    18. regulation of erythrocyte differentiation Source: Ensembl
    19. regulation of transcription, DNA-templated Source: MGI
    20. regulation of transcription from RNA polymerase II promoter Source: MGI
    21. somatic stem cell maintenance Source: MGI
    22. transcription from RNA polymerase II promoter Source: GOC
    23. vasculature development Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor PU.1
    Alternative name(s):
    31 kDa-transforming protein
    SFFV proviral integration 1 protein
    Gene namesi
    Name:Spi1
    Synonyms:Sfpi-1, Sfpi1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:98282. Spi1.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear chromatin Source: BHF-UCL
    2. nucleus Source: BHF-UCL
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Involved in murine acute Friend erythroleukemia. It is a target region for SFFV proviral insertion.

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 272272Transcription factor PU.1PRO_0000204133Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei148 – 1481Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP17433.

    PTM databases

    PhosphoSiteiP17433.

    Expressioni

    Gene expression databases

    ArrayExpressiP17433.
    BgeeiP17433.
    CleanExiMM_SFPI1.
    GenevestigatoriP17433.

    Interactioni

    Subunit structurei

    Binds DNA as a monomer. Interacts with RUNX1 and SPIB By similarity. Interacts with CEBPD and NONO. Interacts with GFI1; the interaction represses SPI1 transcriptional activity.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GFI1Q996842EBI-607588,EBI-949368From a different organism.
    Gfi1P703382EBI-607588,EBI-3954754
    NonoQ99K483EBI-607588,EBI-607499

    Protein-protein interaction databases

    BioGridi203184. 14 interactions.
    IntActiP17433. 5 interactions.
    MINTiMINT-94708.

    Structurei

    Secondary structure

    1
    272
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi174 – 18411
    Turni188 – 1903
    Beta strandi191 – 1955
    Turni196 – 1994
    Beta strandi200 – 2034
    Turni205 – 2073
    Helixi208 – 21912
    Helixi227 – 24014
    Beta strandi241 – 2455
    Beta strandi251 – 2544

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PUEX-ray2.10E/F171-259[»]
    ProteinModelPortaliP17433.
    SMRiP17433. Positions 171-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17433.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG237824.
    HOGENOMiHOG000095520.
    HOVERGENiHBG002474.
    InParanoidiP17433.
    KOiK09438.
    OMAiPRMCLPY.
    PhylomeDBiP17433.
    TreeFamiTF352494.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    [Graphical view]
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17433-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQACKMEGF SLTAPPSDDL VTYDSELYQR PMHDYYSFVG SDGESHSDHY    50
    WDFSAHHVHN NEFENFPENH FTELQSVQPP QLQQLYRHME LEQMHVLDTP 100
    MVPPHTGLSH QVSYMPRMCF PYQTLSPAHQ QSSDEEEGER QSPPLEVSDG 150
    EADGLEPGPG LLHGETGSKK KIRLYQFLLD LLRSGDMKDS IWWVDKDKGT 200
    FQFSSKHKEA LAHRWGIQKG NRKKMTYQKM ARALRNYGKT GEVKKVKKKL 250
    TYQFSGEVLG RGGLAERRLP PH 272
    Length:272
    Mass (Da):31,349
    Last modified:February 1, 2005 - v2
    Checksum:i765E9CC2F374EB6E
    GO

    Sequence cautioni

    The sequence AAB59699.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence CAA35502.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17463 mRNA. Translation: CAA35502.1. Different initiation.
    L03215 mRNA. Translation: AAB59699.1. Different initiation.
    M32370 mRNA. Translation: AAA40024.1.
    M38252 mRNA. Translation: AAA40110.1.
    BC003815 mRNA. Translation: AAH03815.1.
    CCDSiCCDS16425.1.
    PIRiA34693.
    RefSeqiNP_035485.1. NM_011355.1.
    UniGeneiMm.1302.

    Genome annotation databases

    EnsembliENSMUST00000002180; ENSMUSP00000002180; ENSMUSG00000002111.
    GeneIDi20375.
    KEGGimmu:20375.
    UCSCiuc008kuj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17463 mRNA. Translation: CAA35502.1 . Different initiation.
    L03215 mRNA. Translation: AAB59699.1 . Different initiation.
    M32370 mRNA. Translation: AAA40024.1 .
    M38252 mRNA. Translation: AAA40110.1 .
    BC003815 mRNA. Translation: AAH03815.1 .
    CCDSi CCDS16425.1.
    PIRi A34693.
    RefSeqi NP_035485.1. NM_011355.1.
    UniGenei Mm.1302.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PUE X-ray 2.10 E/F 171-259 [» ]
    ProteinModelPortali P17433.
    SMRi P17433. Positions 171-259.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203184. 14 interactions.
    IntActi P17433. 5 interactions.
    MINTi MINT-94708.

    PTM databases

    PhosphoSitei P17433.

    Proteomic databases

    PRIDEi P17433.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002180 ; ENSMUSP00000002180 ; ENSMUSG00000002111 .
    GeneIDi 20375.
    KEGGi mmu:20375.
    UCSCi uc008kuj.1. mouse.

    Organism-specific databases

    CTDi 6688.
    MGIi MGI:98282. Spi1.

    Phylogenomic databases

    eggNOGi NOG237824.
    HOGENOMi HOG000095520.
    HOVERGENi HBG002474.
    InParanoidi P17433.
    KOi K09438.
    OMAi PRMCLPY.
    PhylomeDBi P17433.
    TreeFami TF352494.

    Miscellaneous databases

    EvolutionaryTracei P17433.
    NextBioi 298282.
    PROi P17433.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P17433.
    Bgeei P17433.
    CleanExi MM_SFPI1.
    Genevestigatori P17433.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    [Graphical view ]
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias."
      Moreau-Gachelin F., Ray D., Mattei M.-G., Tambourin P., Tavitian A.
      Oncogene 4:1449-1456(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICFW.
    2. Cited for: SEQUENCE REVISION.
    3. "The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene."
      Klemsz M.J., McKercher S.R., Celada A., van Beveren C., Maki R.A.
      Cell 61:113-124(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor Pu.1."
      Paul R., Schuetze S., Kozak S.L., Kozak C.A., Kabat D.
      J. Virol. 65:464-467(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    6. "Multiple proteins physically interact with PU.1. Transcriptional synergy with NF-IL6 beta (C/EBP delta, CRP3)."
      Nagulapalli S., Pongubala J.M., Atchison M.L.
      J. Immunol. 155:4330-4338(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CEBPD.
    7. "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb."
      Hallier M., Tavitian A., Moreau-Gachelin F.
      J. Biol. Chem. 271:11177-11181(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: RNA-BINDING, FUNCTION, INTERACTION WITH NONO.
    8. "The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction."
      Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C.
      J. Biol. Chem. 282:6473-6483(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GFI1.
    9. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex."
      Kodandapani R., Pio F., Ni C.-Z., Piccialli G., Klemsz M., McKercher S., Maki R.A., Ely K.R.
      Nature 380:456-460(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 171-259.

    Entry informationi

    Entry nameiSPI1_MOUSE
    AccessioniPrimary (citable) accession number: P17433
    Secondary accession number(s): Q99L57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 144 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3