Transcription factor PU.1 - Mus musculus (Mouse)

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P17433 (SPI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 132. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcription factor PU.1

Alternative name(s):
31 kDa-transforming protein
SFFV proviral integration 1 protein

Gene names

Name:	Spi1
Synonyms:	Sfpi-1, Sfpi1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing. Ref.7
Subunit structure	Binds DNA as a monomer. Interacts with RUNX1 and SPIB By similarity. Interacts with CEBPD and NONO. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus.
Involvement in disease	Involved in murine acute Friend erythroleukemia. It is a target region for SFFV proviral insertion.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.
Sequence caution	The sequence AAB59699.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA35502.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Disease	Proto-oncogene
Ligand	DNA-binding RNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anatomical structure regression Inferred from mutant phenotype PubMed 16163358. Source: MGI erythrocyte differentiation Inferred from mutant phenotype PubMed 9133423. Source: MGI granulocyte differentiation Inferred from direct assay PubMed 11980719. Source: MGI histone H3 acetylation Inferred from electronic annotation. Source: Compara hypermethylation of CpG island Inferred from electronic annotation. Source: Compara induction of apoptosis Inferred from mutant phenotype PubMed 16163358. Source: MGI lymphocyte differentiation Inferred from direct assay PubMed 11980719. Source: MGI lymphoid progenitor cell differentiation Inferred from mutant phenotype PubMed 9133423. Source: MGI macrophage differentiation Inferred from direct assay PubMed 11980719. Source: MGI myeloid dendritic cell differentiation Inferred from mutant phenotype PubMed 10648399. Source: MGI negative regulation of MHC class II biosynthetic process Inferred from direct assay PubMed 7592651. Source: BHF-UCL negative regulation of gene expression, epigenetic Inferred from electronic annotation. Source: Compara negative regulation of histone H4 acetylation Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara regulation of erythrocyte differentiation Inferred from electronic annotation. Source: Compara somatic stem cell maintenance Inferred from direct assay PubMed 19379700. Source: MGI vasculature development Inferred from mutant phenotype PubMed 16163358. Source: MGI
Cellular_component	nuclear chromatin Inferred from direct assay PubMed 15187136. Source: BHF-UCL transcription factor complex Inferred by curator PubMed 11980719. Source: MGI
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II distal enhancer sequence-specific DNA binding Inferred from direct assay PubMed 15304486. Source: BHF-UCL RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay PubMed 15304486. Source: BHF-UCL core promoter binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GFI1	Q99684	2	EBI-607588,EBI-949368	From a different organism.
Gfi1	P70338	2	EBI-607588,EBI-3954754
Nono	Q99K48	3	EBI-607588,EBI-607499

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 272

272

Transcription factor PU.1

PRO_0000204133

Regions

DNA binding

172 – 255

ETS

Amino acid modifications

Modified residue

148

Phosphoserine Ref.9

Secondary structure

272

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P17433 [UniParc].

Last modified February 1, 2005. Version 2.
Checksum: 765E9CC2F374EB6E
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FASTA

272

31,349

        10         20         30         40         50         60 
MLQACKMEGF SLTAPPSDDL VTYDSELYQR PMHDYYSFVG SDGESHSDHY WDFSAHHVHN 

        70         80         90        100        110        120 
NEFENFPENH FTELQSVQPP QLQQLYRHME LEQMHVLDTP MVPPHTGLSH QVSYMPRMCF 

       130        140        150        160        170        180 
PYQTLSPAHQ QSSDEEEGER QSPPLEVSDG EADGLEPGPG LLHGETGSKK KIRLYQFLLD 

       190        200        210        220        230        240 
LLRSGDMKDS IWWVDKDKGT FQFSSKHKEA LAHRWGIQKG NRKKMTYQKM ARALRNYGKT 

       250        260        270 
GEVKKVKKKL TYQFSGEVLG RGGLAERRLP PH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias." Moreau-Gachelin F., Ray D., Mattei M.-G., Tambourin P., Tavitian A. Oncogene 4:1449-1456(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICFW.
[2]	Erratum Moreau-Gachelin F., Ray D., Mattei M.-G., Tambourin P., Tavitian A. Oncogene 5:941-941(1990) Cited for: SEQUENCE REVISION.
[3]	"The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene." Klemsz M.J., McKercher S.R., Celada A., van Beveren C., Maki R.A. Cell 61:113-124(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor Pu.1." Paul R., Schuetze S., Kozak S.L., Kozak C.A., Kabat D. J. Virol. 65:464-467(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[6]	"Multiple proteins physically interact with PU.1. Transcriptional synergy with NF-IL6 beta (C/EBP delta, CRP3)." Nagulapalli S., Pongubala J.M., Atchison M.L. J. Immunol. 155:4330-4338(1995) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CEBPD.
[7]	"The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb." Hallier M., Tavitian A., Moreau-Gachelin F. J. Biol. Chem. 271:11177-11181(1996) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING, FUNCTION, INTERACTION WITH NONO.
[8]	"The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction." Dahl R., Iyer S.R., Owens K.S., Cuylear D.D., Simon M.C. J. Biol. Chem. 282:6473-6483(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GFI1.
[9]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148, MASS SPECTROMETRY. Tissue: Macrophage.
[10]	"A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex." Kodandapani R., Pio F., Ni C.-Z., Piccialli G., Klemsz M., McKercher S., Maki R.A., Ely K.R. Nature 380:456-460(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 171-259.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X17463 mRNA. Translation: CAA35502.1. Different initiation.
L03215 mRNA. Translation: AAB59699.1. Different initiation.
M32370 mRNA. Translation: AAA40024.1.
M38252 mRNA. Translation: AAA40110.1.
BC003815 mRNA. Translation: AAH03815.1.

IPI

IPI00116335.

PIR

A34693.

RefSeq

NP_035485.1. NM_011355.1.

UniGene

Mm.1302.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PUE	X-ray	2.10	E/F	171-259	[»]

ProteinModelPortal

P17433.

SMR

P17433. Positions 171-259.

ModBase

Search...

Protein-protein interaction databases

IntAct

P17433. 5 interactions.

PTM databases

PhosphoSite

P17433.

Proteomic databases

PRIDE

P17433.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000002180; ENSMUSP00000002180; ENSMUSG00000002111.

GeneID

20375.

KEGG

mmu:20375.

Organism-specific databases

CTD

20375.

MGI

MGI:98282. Sfpi1.

Phylogenomic databases

eggNOG

NOG237824.

HOGENOM

HOG000095520.

HOVERGEN

HBG002474.

InParanoid

P17433.

K09438.

OMA

PRMCLPY.

OrthoDB

EOG4V9TRC.

Gene expression databases

ArrayExpress

P17433.

Bgee

P17433.

CleanEx

MM_SFPI1.

Genevestigator

P17433.

GermOnline

ENSMUSG00000002111. Mus musculus.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00178. Ets. 1 hit.
[Graphical view]

PRINTS

PR00454. ETSDOMAIN.

SMART

SM00413. ETS. 1 hit.
[Graphical view]

PROSITE

PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P17433.

NextBio

298282.

SOURCE

Search...

Entry information

Entry name

SPI1_MOUSE

Accession

Primary (citable) accession number: P17433
Secondary accession number(s): Q99L57

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 132 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents