Reviewed, UniProtKB/Swiss-Prot P17432 (MASY_GOSHI)

Names and origin

Protein names	Recommended name:     Malate synthase, glyoxysomal     EC=2.3.3.9
Organism	Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifier	3635 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Malate synthase, glyoxysomal		PRO_0000166869
Regions
	Motif	565 – 567	3	Microbody targeting signal Potential
Sites
	Active site	182	1	Proton acceptor By similarity
	Active site	468	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17432-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: AC69D3BE20C91204	FASTA	567	64,669
	10 20 30 40 50 60 MIGLGSYGYT APSSKKINAY DVPQGVDIRG RFDEEFAKIL TKDALQFVAD LQREFRNHIK 70 80 90 100 110 120 YAMECRKEAK RRYNEGALPG FDPATRYIRE GKWTCVPFPP AVADRRVEIT GPVERKMIIN 130 140 150 160 170 180 ALNSGAKVFM ADFEDALSPS WENLMRGQIN LKDAVEGTIT FNDKARNRVY KLNNEIAKLF 190 200 210 220 230 240 VRPRGWHLPE AHIFIDGEPA TGCLVDFGLY FYHNYATFRN TQGQGFGPFF YLPKMENSRE 250 260 270 280 290 300 AKIWNSVFEK AEKMAGIEKG SIRATVLIET LPAVFQMDEI FYELRDHSVG LNCGRWDYIF 310 320 330 340 350 360 SYVKTFQGHP DRLLPDRGQV GMTQHFMRSY SDLLIRTCHR RGVHAMGGMA AQIPIRDDPT 370 380 390 400 410 420 ANEAAFELVR KDKQREVKAG HDGTWAAHPG LIKTCMEVFT NNMGNTPNQI ETVKRDYASN 430 440 450 460 470 480 LTEDDLLQRP RGVRTMEGLR LNTRVGIQYL AAWLTGSGSV PLYNLMEDAA TAEISRVQIW 490 500 510 520 530 540 QWLKYGVELD GDGLGVRVNH VFGRVVEEEM ARIEREVGKE KFKKGMYKEA CKIFTRQCTA 550 560 STLDDFLTLD AYNYIVIHHP KDVSSKL

References

[1]	"Nucleotide sequence of cottonseed malate synthase." Turley R.B., Choe S.M., Ni W., Trelease R.N. Nucleic Acids Res. 18:3643-3643(1990) [PubMed: 2362818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52305 mRNA. Translation: CAA36546.1.
PIR	SYCNMU. S12735.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 1857.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_GOSHI

Accession

Primary (citable) accession number: P17432

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	October 13, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)