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P17425 (HMCS1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Short name=HMG-CoA synthase
EC=2.3.3.10
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene names
Name:Hmgcs1
Synonyms:Hmgcs
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Catalytic activity

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
   Biological processCholesterol biosynthesis
Cholesterol metabolism
Lipid biosynthesis
Lipid metabolism
Steroid biosynthesis
Steroid metabolism
Sterol biosynthesis
Sterol metabolism
   Cellular componentCytoplasm
   Molecular functionTransferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processbrain development

Inferred from expression pattern PubMed 6133227. Source: RGD

cellular response to cholesterol

Inferred from expression pattern PubMed 18303831. Source: RGD

cellular response to follicle-stimulating hormone stimulus

Inferred from expression pattern PubMed 19196834. Source: RGD

cellular response to organic cyclic compound

Inferred from expression pattern PubMed 9409294. Source: RGD

cholesterol biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

isoprenoid biosynthetic process

Inferred from electronic annotation. Source: InterPro

liver development

Inferred from expression pattern PubMed 5166591. Source: RGD

male gonad development

Inferred from expression pattern PubMed 11792726. Source: RGD

response to cholesterol

Inferred from expression pattern PubMed 2903839. Source: RGD

response to drug

Inferred from expression pattern PubMed 16020911PubMed 17311803. Source: RGD

response to gonadotropin

Inferred from expression pattern PubMed 15459111. Source: RGD

response to hormone

Inferred from expression pattern PubMed 8093833. Source: RGD

response to lipoprotein particle

Inferred from expression pattern PubMed 16260. Source: RGD

response to low light intensity stimulus

Inferred from expression pattern PubMed 1683769. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 19527300. Source: RGD

response to organic substance

Inferred from expression pattern PubMed 3670308. Source: RGD

response to organonitrogen compound

Inferred from direct assay PubMed 16260. Source: RGD

response to purine-containing compound

Inferred from expression pattern PubMed 16260. Source: RGD

response to tellurium ion

Inferred from expression pattern PubMed 10642751. Source: RGD

response to vitamin E

Inferred from expression pattern PubMed 15753162. Source: RGD

   Cellular_componentcytosol

Inferred from direct assay PubMed 14984735. Source: RGD

nucleus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiondrug binding

Inferred from direct assay PubMed 14984735. Source: RGD

hydroxymethylglutaryl-CoA synthase activity

Inferred from direct assay PubMed 8094614. Source: RGD

isomerase activity

Inferred from direct assay PubMed 17764185. Source: RGD

organic acid binding

Inferred from direct assay PubMed 8094614. Source: RGD

protein homodimerization activity

Inferred from direct assay PubMed 14984735. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Hydroxymethylglutaryl-CoA synthase, cytoplasmic
PRO_0000213750

Sites

Active site951Proton donor/acceptor By similarity
Active site1291Acyl-thioester intermediate By similarity
Active site2641Proton donor/acceptor By similarity

Amino acid modifications

Modified residue41Phosphoserine By similarity
Modified residue461N6-acetyllysine By similarity
Modified residue2731N6-acetyllysine By similarity
Modified residue4951Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P17425 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: CB213A27B0C177CB

FASTA52057,434
        10         20         30         40         50         60 
MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG LGQARMGFCT 

        70         80         90        100        110        120 
DREDINSLCL TVVQKLMERN SLSYDCIGRL EVGTETIIDK SKSVKSNLMQ LFEESGNTDI 

       130        140        150        160        170        180 
EGIDTTNACY GGTAAVFNAV NWIESSSWDG RYALVVAGDI AIYASGNARP TGGVGAVALL 

       190        200        210        220        230        240 
IGPNAPVIFD RGLRGTHMQH AYDFYKPDML SEYPVVDGKL SIQCYLSALD RCYSVYRKKI 

       250        260        270        280        290        300 
RAQWQKEGKD KDFTLNDFGF MIFHSPYCKL VQKSLARMFL NDFLNDQNRD KNSIYSGLEA 

       310        320        330        340        350        360 
FGDVKLEDTY FDRDVEKAFM KASAELFNQK TKASLLVSNQ NGNMYTSSVY GSLASVLAQY 

       370        380        390        400        410        420 
SPQQLAGKRI GVFSYGSGLA ATLYSLKVTQ DATPGSALDK ITASLCDLKS RLDSRTCVAP 

       430        440        450        460        470        480 
DVFAENMKLR EDTHHLANYI PQCSIDSLFE GTWYLVRVDE KHRRTYARRP STNDHSLDEG 

       490        500        510        520 
VGLVHSNTAT EHIPSPAKKV PRLPATSGEP ESAVISNGEH 

« Hide

References

[1]"Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase."
Ayte J., Gil-Gomez G., Hegardt F.G.
Nucleic Acids Res. 18:3642-3642(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]Lubec G., Afjehi-Sadat L., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 90-100; 220-231; 278-289; 292-305; 370-387 AND 401-409, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52625 mRNA. Translation: CAA36852.1.
PIRS12736.
RefSeqNP_058964.1. NM_017268.1.
XP_006232021.1. XM_006231959.1.
UniGeneRn.5106.

3D structure databases

ProteinModelPortalP17425.
SMRP17425. Positions 16-470.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023554.

Chemistry

BindingDBP17425.
ChEMBLCHEMBL2189.
GuidetoPHARMACOLOGY638.

PTM databases

PhosphoSiteP17425.

Proteomic databases

PaxDbP17425.
PRIDEP17425.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023554; ENSRNOP00000023554; ENSRNOG00000016552.
GeneID29637.
KEGGrno:29637.

Organism-specific databases

CTD3157.
RGD70970. Hmgcs1.

Phylogenomic databases

eggNOGCOG3425.
GeneTreeENSGT00390000006096.
HOGENOMHOG000012351.
HOVERGENHBG051912.
InParanoidP17425.
KOK01641.
OMARDFTLND.
OrthoDBEOG741Z1W.
PhylomeDBP17425.
TreeFamTF105361.

Enzyme and pathway databases

BRENDA2.3.3.10. 5301.
UniPathwayUPA00058; UER00102.

Gene expression databases

GenevestigatorP17425.

Family and domain databases

Gene3D3.40.47.10. 1 hit.
InterProIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 3 hits.
TIGRFAMsTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio609884.
PROP17425.

Entry information

Entry nameHMCS1_RAT
AccessionPrimary (citable) accession number: P17425
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways