Reviewed,
UniProtKB/Swiss-Prot P17425 (HMCS1_RAT)
Last modified
February 9, 2010.
Version 89.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Hydroxymethylglutaryl-CoA synthase, cytoplasmic Short name=HMG-CoA synthase EC=2.3.3.10 Alternative name(s): 3-hydroxy-3-methylglutaryl coenzyme A synthase | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 520 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. |
| Catalytic activity | Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA. |
| Pathway | |
| Subcellular location | |
| Sequence similarities | Belongs to the HMG-CoA synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis |
| Cellular component | Cytoplasm |
| Molecular function | Transferase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic processInferred from electronic annotation. Source: InterPro response to drugInferred from expression pattern. Source: RGD response to organic nitrogenInferred from direct assay. Source: RGD |
| Cellular component | cytosol Ref.1 Traceable author statement. Source: RGD |
| Molecular function | hydroxymethylglutaryl-CoA synthase activity Ref.1 Traceable author statement. Source: RGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 520 | 520 | Hydroxymethylglutaryl-CoA synthase, cytoplasmic | PRO_0000213750 | |||||
Sites | |||||||||
| Active site | 129 | 1 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 4 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 46 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 246 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 273 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 321 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 495 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 516 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| [1] | "Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase." Ayte J., Gil-Gomez G., Hegardt F.G. Nucleic Acids Res. 18:3642-3642(1990) [PubMed: 1972979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver. |
| [2] | Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 90-100; 220-231; 278-289; 292-305; 370-387 AND 401-409, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X52625 mRNA. Translation: CAA36852.1. |
| IPI | IPI00188158. |
| PIR | S12736. |
| RefSeq | NP_058964.1. |
| UniGene | Rn.5106 |
3D structure databases | |
| SMR | P17425. Positions 16-470. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P17425. |
PTM databases | |
| PhosphoSite | P17425. |
Proteomic databases | |
| PRIDE | P17425. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000023554; ENSRNOP00000023554; ENSRNOG00000016552; Rattus norvegicus. [Genome view] |
| GeneID | 29637. |
| KEGG | rno:29637. |
| NMPDR | fig|10116.3.peg.15558. |
| UCSC | NM_017268. rat. |
Organism-specific databases | |
| CTD | 29637. |
| RGD | 70970. Hmgcs1. |
Phylogenomic databases | |
| eggNOG | roNOG08859. |
| HOVERGEN | P17425. |
| InParanoid | P17425. |
| OMA | MKASAEL. |
| OrthoDB | EOG9S4S1K. |
| PhylomeDB | P17425. |
Enzyme and pathway databases | |
| BRENDA | 2.3.3.10. 248. |
Gene expression databases | |
| ArrayExpress | P17425. |
| Genevestigator | P17425. |
| GermOnline | ENSRNOG00000016552. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. [Graphical view] |
| Pfam | PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01833. HMG-CoA-S_euk. 1 hit. |
| PROSITE | PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 609884. |
Entry information
| Entry name | HMCS1_RAT | ||||||||
| Accession | Primary (citable) accession number: P17425 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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