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P17425

- HMCS1_RAT

UniProt

P17425 - HMCS1_RAT

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Protein

Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene
Hmgcs1, Hmgcs
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Proton donor/acceptor By similarity
Active sitei129 – 1291Acyl-thioester intermediate By similarity
Active sitei264 – 2641Proton donor/acceptor By similarity

GO - Molecular functioni

  1. drug binding Source: RGD
  2. hydroxymethylglutaryl-CoA synthase activity Source: RGD
  3. isomerase activity Source: RGD
  4. organic acid binding Source: RGD
  5. protein homodimerization activity Source: RGD

GO - Biological processi

  1. brain development Source: RGD
  2. cellular response to cholesterol Source: RGD
  3. cellular response to follicle-stimulating hormone stimulus Source: RGD
  4. cellular response to organic cyclic compound Source: RGD
  5. cholesterol biosynthetic process Source: UniProtKB-KW
  6. isoprenoid biosynthetic process Source: InterPro
  7. liver development Source: RGD
  8. male gonad development Source: RGD
  9. response to cholesterol Source: RGD
  10. response to drug Source: RGD
  11. response to gonadotropin Source: RGD
  12. response to hormone Source: RGD
  13. response to lipoprotein particle Source: RGD
  14. response to low light intensity stimulus Source: RGD
  15. response to organic cyclic compound Source: RGD
  16. response to organic substance Source: RGD
  17. response to organonitrogen compound Source: RGD
  18. response to purine-containing compound Source: RGD
  19. response to tellurium ion Source: RGD
  20. response to vitamin E Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.3.10. 5301.
ReactomeiREACT_196408. PPARA activates gene expression.
REACT_198613. Activation of gene expression by SREBF (SREBP).
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:Hmgcs1
Synonyms:Hmgcs
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi70970. Hmgcs1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RGD
  2. nucleus Source: Ensembl
  3. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Hydroxymethylglutaryl-CoA synthase, cytoplasmicPRO_0000213750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41Phosphoserine By similarity
Modified residuei46 – 461N6-acetyllysine By similarity
Modified residuei273 – 2731N6-acetyllysine By similarity
Modified residuei495 – 4951Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP17425.
PRIDEiP17425.

PTM databases

PhosphoSiteiP17425.

Expressioni

Gene expression databases

GenevestigatoriP17425.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023554.

Structurei

3D structure databases

ProteinModelPortaliP17425.
SMRiP17425. Positions 16-470.

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.

Phylogenomic databases

eggNOGiCOG3425.
GeneTreeiENSGT00390000006096.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiP17425.
KOiK01641.
OMAiRDFTLND.
OrthoDBiEOG741Z1W.
PhylomeDBiP17425.
TreeFamiTF105361.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17425-1 [UniParc]FASTAAdd to Basket

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MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG    50
LGQARMGFCT DREDINSLCL TVVQKLMERN SLSYDCIGRL EVGTETIIDK 100
SKSVKSNLMQ LFEESGNTDI EGIDTTNACY GGTAAVFNAV NWIESSSWDG 150
RYALVVAGDI AIYASGNARP TGGVGAVALL IGPNAPVIFD RGLRGTHMQH 200
AYDFYKPDML SEYPVVDGKL SIQCYLSALD RCYSVYRKKI RAQWQKEGKD 250
KDFTLNDFGF MIFHSPYCKL VQKSLARMFL NDFLNDQNRD KNSIYSGLEA 300
FGDVKLEDTY FDRDVEKAFM KASAELFNQK TKASLLVSNQ NGNMYTSSVY 350
GSLASVLAQY SPQQLAGKRI GVFSYGSGLA ATLYSLKVTQ DATPGSALDK 400
ITASLCDLKS RLDSRTCVAP DVFAENMKLR EDTHHLANYI PQCSIDSLFE 450
GTWYLVRVDE KHRRTYARRP STNDHSLDEG VGLVHSNTAT EHIPSPAKKV 500
PRLPATSGEP ESAVISNGEH 520
Length:520
Mass (Da):57,434
Last modified:August 1, 1990 - v1
Checksum:iCB213A27B0C177CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52625 mRNA. Translation: CAA36852.1.
PIRiS12736.
RefSeqiNP_058964.1. NM_017268.1.
XP_006232021.1. XM_006231959.1.
UniGeneiRn.5106.

Genome annotation databases

EnsembliENSRNOT00000023554; ENSRNOP00000023554; ENSRNOG00000016552.
GeneIDi29637.
KEGGirno:29637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52625 mRNA. Translation: CAA36852.1 .
PIRi S12736.
RefSeqi NP_058964.1. NM_017268.1.
XP_006232021.1. XM_006231959.1.
UniGenei Rn.5106.

3D structure databases

ProteinModelPortali P17425.
SMRi P17425. Positions 16-470.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000023554.

Chemistry

BindingDBi P17425.
ChEMBLi CHEMBL2189.
GuidetoPHARMACOLOGYi 638.

PTM databases

PhosphoSitei P17425.

Proteomic databases

PaxDbi P17425.
PRIDEi P17425.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000023554 ; ENSRNOP00000023554 ; ENSRNOG00000016552 .
GeneIDi 29637.
KEGGi rno:29637.

Organism-specific databases

CTDi 3157.
RGDi 70970. Hmgcs1.

Phylogenomic databases

eggNOGi COG3425.
GeneTreei ENSGT00390000006096.
HOGENOMi HOG000012351.
HOVERGENi HBG051912.
InParanoidi P17425.
KOi K01641.
OMAi RDFTLND.
OrthoDBi EOG741Z1W.
PhylomeDBi P17425.
TreeFami TF105361.

Enzyme and pathway databases

UniPathwayi UPA00058 ; UER00102 .
BRENDAi 2.3.3.10. 5301.
Reactomei REACT_196408. PPARA activates gene expression.
REACT_198613. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

NextBioi 609884.
PROi P17425.

Gene expression databases

Genevestigatori P17425.

Family and domain databases

Gene3Di 3.40.47.10. 1 hit.
InterProi IPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 3 hits.
TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase."
    Ayte J., Gil-Gomez G., Hegardt F.G.
    Nucleic Acids Res. 18:3642-3642(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 90-100; 220-231; 278-289; 292-305; 370-387 AND 401-409, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiHMCS1_RAT
AccessioniPrimary (citable) accession number: P17425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 3, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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