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P17425

- HMCS1_RAT

UniProt

P17425 - HMCS1_RAT

Protein

Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene

Hmgcs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

    Catalytic activityi

    Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951Proton donor/acceptorPROSITE-ProRule annotation
    Active sitei129 – 1291Acyl-thioester intermediatePROSITE-ProRule annotation
    Active sitei264 – 2641Proton donor/acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. hydroxymethylglutaryl-CoA synthase activity Source: RGD
    3. isomerase activity Source: RGD
    4. organic acid binding Source: RGD
    5. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. brain development Source: RGD
    2. cellular response to cholesterol Source: RGD
    3. cellular response to follicle-stimulating hormone stimulus Source: RGD
    4. cellular response to organic cyclic compound Source: RGD
    5. cholesterol biosynthetic process Source: UniProtKB-KW
    6. isoprenoid biosynthetic process Source: InterPro
    7. liver development Source: RGD
    8. male gonad development Source: RGD
    9. response to cholesterol Source: RGD
    10. response to drug Source: RGD
    11. response to gonadotropin Source: RGD
    12. response to hormone Source: RGD
    13. response to lipoprotein particle Source: RGD
    14. response to low light intensity stimulus Source: RGD
    15. response to organic cyclic compound Source: RGD
    16. response to organic substance Source: RGD
    17. response to organonitrogen compound Source: RGD
    18. response to purine-containing compound Source: RGD
    19. response to tellurium ion Source: RGD
    20. response to vitamin E Source: RGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Enzyme and pathway databases

    BRENDAi2.3.3.10. 5301.
    ReactomeiREACT_196408. PPARA activates gene expression.
    REACT_198613. Activation of gene expression by SREBF (SREBP).
    UniPathwayiUPA00058; UER00102.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylglutaryl-CoA synthase, cytoplasmic (EC:2.3.3.10)
    Short name:
    HMG-CoA synthase
    Alternative name(s):
    3-hydroxy-3-methylglutaryl coenzyme A synthase
    Gene namesi
    Name:Hmgcs1
    Synonyms:Hmgcs
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi70970. Hmgcs1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. nucleus Source: Ensembl
    3. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 520520Hydroxymethylglutaryl-CoA synthase, cytoplasmicPRO_0000213750Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei4 – 41PhosphoserineBy similarity
    Modified residuei46 – 461N6-acetyllysineBy similarity
    Modified residuei273 – 2731N6-acetyllysineBy similarity
    Modified residuei495 – 4951PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP17425.
    PRIDEiP17425.

    PTM databases

    PhosphoSiteiP17425.

    Expressioni

    Gene expression databases

    GenevestigatoriP17425.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000023554.

    Structurei

    3D structure databases

    ProteinModelPortaliP17425.
    SMRiP17425. Positions 16-470.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMG-CoA synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG3425.
    GeneTreeiENSGT00390000006096.
    HOGENOMiHOG000012351.
    HOVERGENiHBG051912.
    InParanoidiP17425.
    KOiK01641.
    OMAiRDFTLND.
    OrthoDBiEOG741Z1W.
    PhylomeDBiP17425.
    TreeFamiTF105361.

    Family and domain databases

    Gene3Di3.40.47.10. 1 hit.
    InterProiIPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 3 hits.
    TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17425-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG    50
    LGQARMGFCT DREDINSLCL TVVQKLMERN SLSYDCIGRL EVGTETIIDK 100
    SKSVKSNLMQ LFEESGNTDI EGIDTTNACY GGTAAVFNAV NWIESSSWDG 150
    RYALVVAGDI AIYASGNARP TGGVGAVALL IGPNAPVIFD RGLRGTHMQH 200
    AYDFYKPDML SEYPVVDGKL SIQCYLSALD RCYSVYRKKI RAQWQKEGKD 250
    KDFTLNDFGF MIFHSPYCKL VQKSLARMFL NDFLNDQNRD KNSIYSGLEA 300
    FGDVKLEDTY FDRDVEKAFM KASAELFNQK TKASLLVSNQ NGNMYTSSVY 350
    GSLASVLAQY SPQQLAGKRI GVFSYGSGLA ATLYSLKVTQ DATPGSALDK 400
    ITASLCDLKS RLDSRTCVAP DVFAENMKLR EDTHHLANYI PQCSIDSLFE 450
    GTWYLVRVDE KHRRTYARRP STNDHSLDEG VGLVHSNTAT EHIPSPAKKV 500
    PRLPATSGEP ESAVISNGEH 520
    Length:520
    Mass (Da):57,434
    Last modified:August 1, 1990 - v1
    Checksum:iCB213A27B0C177CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52625 mRNA. Translation: CAA36852.1.
    PIRiS12736.
    RefSeqiNP_058964.1. NM_017268.1.
    XP_006232021.1. XM_006231959.1.
    UniGeneiRn.5106.

    Genome annotation databases

    EnsembliENSRNOT00000023554; ENSRNOP00000023554; ENSRNOG00000016552.
    GeneIDi29637.
    KEGGirno:29637.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52625 mRNA. Translation: CAA36852.1 .
    PIRi S12736.
    RefSeqi NP_058964.1. NM_017268.1.
    XP_006232021.1. XM_006231959.1.
    UniGenei Rn.5106.

    3D structure databases

    ProteinModelPortali P17425.
    SMRi P17425. Positions 16-470.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000023554.

    Chemistry

    BindingDBi P17425.
    ChEMBLi CHEMBL2189.
    GuidetoPHARMACOLOGYi 638.

    PTM databases

    PhosphoSitei P17425.

    Proteomic databases

    PaxDbi P17425.
    PRIDEi P17425.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023554 ; ENSRNOP00000023554 ; ENSRNOG00000016552 .
    GeneIDi 29637.
    KEGGi rno:29637.

    Organism-specific databases

    CTDi 3157.
    RGDi 70970. Hmgcs1.

    Phylogenomic databases

    eggNOGi COG3425.
    GeneTreei ENSGT00390000006096.
    HOGENOMi HOG000012351.
    HOVERGENi HBG051912.
    InParanoidi P17425.
    KOi K01641.
    OMAi RDFTLND.
    OrthoDBi EOG741Z1W.
    PhylomeDBi P17425.
    TreeFami TF105361.

    Enzyme and pathway databases

    UniPathwayi UPA00058 ; UER00102 .
    BRENDAi 2.3.3.10. 5301.
    Reactomei REACT_196408. PPARA activates gene expression.
    REACT_198613. Activation of gene expression by SREBF (SREBP).

    Miscellaneous databases

    NextBioi 609884.
    PROi P17425.

    Gene expression databases

    Genevestigatori P17425.

    Family and domain databases

    Gene3Di 3.40.47.10. 1 hit.
    InterProi IPR000590. HMG_CoA_synt_AS.
    IPR013746. HMG_CoA_synt_C_dom.
    IPR013528. HMG_CoA_synth_N.
    IPR010122. HMG_CoA_synthase_euk.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF08540. HMG_CoA_synt_C. 1 hit.
    PF01154. HMG_CoA_synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 3 hits.
    TIGRFAMsi TIGR01833. HMG-CoA-S_euk. 1 hit.
    PROSITEi PS01226. HMG_COA_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase."
      Ayte J., Gil-Gomez G., Hegardt F.G.
      Nucleic Acids Res. 18:3642-3642(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 90-100; 220-231; 278-289; 292-305; 370-387 AND 401-409, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.

    Entry informationi

    Entry nameiHMCS1_RAT
    AccessioniPrimary (citable) accession number: P17425
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3