Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonucleoside-diphosphate reductase 2 subunit beta

Gene

nrdF

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationNote: Binds 2 iron ions per subunit.

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Iron 11
Metal bindingi98Iron 11
Metal bindingi98Iron 21
Metal bindingi101Iron 11
Active sitei105PROSITE-ProRule annotation1
Metal bindingi158Iron 21
Metal bindingi192Iron 21
Metal bindingi195Iron 21

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13836.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase 2 subunit beta (EC:1.17.4.1)
Alternative name(s):
R2F protein
Ribonucleotide reductase 2
Gene namesi
Name:nrdF
Ordered Locus Names:STM2808
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001904891 – 319Ribonucleoside-diphosphate reductase 2 subunit betaAdd BLAST319

Proteomic databases

PaxDbiP17424.
PRIDEiP17424.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi99287.STM2808.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 28Combined sources12
Helixi33 – 35Combined sources3
Helixi38 – 41Combined sources4
Helixi42 – 46Combined sources5
Helixi50 – 72Combined sources23
Helixi74 – 79Combined sources6
Helixi85 – 112Combined sources28
Helixi115 – 127Combined sources13
Helixi129 – 143Combined sources15
Helixi147 – 159Combined sources13
Turni160 – 162Combined sources3
Helixi163 – 174Combined sources12
Helixi179 – 208Combined sources30
Helixi212 – 240Combined sources29
Turni241 – 244Combined sources4
Helixi247 – 264Combined sources18
Turni273 – 275Combined sources3
Helixi280 – 286Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R2FX-ray2.10A/B1-319[»]
2BQ1X-ray4.00I/J1-319[»]
2R2FX-ray2.25A/B1-319[»]
ProteinModelPortaliP17424.
SMRiP17424.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17424.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107R25. Bacteria.
COG0208. LUCA.
HOGENOMiHOG000246154.
KOiK00526.
OMAiMNLGQEP.
PhylomeDBiP17424.

Family and domain databases

CDDicd01049. RNRR2. 1 hit.
Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR033909. RNR_small.
IPR030475. RNR_small_AS.
IPR000358. RNR_small_fam.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PIRSFiPIRSF000355. NrdB. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSRISAIN WNKIQDDKDL EVWNRLTSNF WLPEKVPLSN DIPAWQTLSA
60 70 80 90 100
AEQQLTIRVF TGLTLLDTIQ NIAGAPSLMA DAITPHEEAV LSNISFMEAV
110 120 130 140 150
HARSYSSIFS TLCQTKEVDA AYAWSEENPP LQRKAQIILA HYVSDEPLKK
160 170 180 190 200
KIASVFLESF LFYSGFWLPM YFSSRGKLTN TADLIRLIIR DEAVHGYYIG
210 220 230 240 250
YKYQIALQKL SAIEREELKL FALDLLMELY DNEIRYTEAL YAETGWVNDV
260 270 280 290 300
KAFLCYNANK ALMNLGYEAL FPPEMADVNP AILAALSPNA DENHDFFSGS
310
GSSYVMGKTV ETEDEDWNF
Length:319
Mass (Da):36,232
Last modified:October 1, 1994 - v2
Checksum:iAFBB6E02A57C959A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286L → V in AAA88620 (PubMed:2548994).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73226 Genomic DNA. Translation: CAA51695.1.
AE006468 Genomic DNA. Translation: AAL21693.1.
X52693 Genomic DNA. Translation: CAA36920.1.
M26063 Genomic DNA. Translation: AAA88620.1.
PIRiS34272.
RefSeqiNP_461734.1. NC_003197.1.
WP_000777903.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21693; AAL21693; STM2808.
GeneIDi1254331.
KEGGistm:STM2808.
PATRICi32384298. VBISalEnt20916_2966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73226 Genomic DNA. Translation: CAA51695.1.
AE006468 Genomic DNA. Translation: AAL21693.1.
X52693 Genomic DNA. Translation: CAA36920.1.
M26063 Genomic DNA. Translation: AAA88620.1.
PIRiS34272.
RefSeqiNP_461734.1. NC_003197.1.
WP_000777903.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R2FX-ray2.10A/B1-319[»]
2BQ1X-ray4.00I/J1-319[»]
2R2FX-ray2.25A/B1-319[»]
ProteinModelPortaliP17424.
SMRiP17424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2808.

Proteomic databases

PaxDbiP17424.
PRIDEiP17424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21693; AAL21693; STM2808.
GeneIDi1254331.
KEGGistm:STM2808.
PATRICi32384298. VBISalEnt20916_2966.

Phylogenomic databases

eggNOGiENOG4107R25. Bacteria.
COG0208. LUCA.
HOGENOMiHOG000246154.
KOiK00526.
OMAiMNLGQEP.
PhylomeDBiP17424.

Enzyme and pathway databases

UniPathwayiUPA00326.
BioCyciMetaCyc:MONOMER-13836.

Miscellaneous databases

EvolutionaryTraceiP17424.

Family and domain databases

CDDicd01049. RNRR2. 1 hit.
Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR033909. RNR_small.
IPR030475. RNR_small_AS.
IPR000358. RNR_small_fam.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PIRSFiPIRSF000355. NrdB. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR4_SALTY
AccessioniPrimary (citable) accession number: P17424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.