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Protein

Homoserine kinase

Gene

THR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.By similarity

Catalytic activityi

ATP + L-homoserine = ADP + O-phospho-L-homoserine.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
  4. Homoserine kinase (THR1)
  5. Threonine synthase (THR4)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 11411ATPSequence analysisAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • homoserine kinase activity Source: SGD

GO - Biological processi

  • homoserine metabolic process Source: SGD
  • isoleucine metabolic process Source: SGD
  • methionine metabolic process Source: SGD
  • threonine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YHR025W-MONOMER.
UniPathwayiUPA00050; UER00064.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine kinase (EC:2.7.1.39)
Short name:
HK
Short name:
HSK
Gene namesi
Name:THR1
Ordered Locus Names:YHR025W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR025W.
SGDiS000001067. THR1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 357356Homoserine kinasePRO_0000156656Add
BLAST

Proteomic databases

MaxQBiP17423.
PeptideAtlasiP17423.

PTM databases

iPTMnetiP17423.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi36456. 104 interactions.
DIPiDIP-4305N.
IntActiP17423. 9 interactions.
MINTiMINT-561221.

Structurei

3D structure databases

ProteinModelPortaliP17423.
SMRiP17423. Positions 4-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000247199.
InParanoidiP17423.
KOiK00872.
OMAiCANRIPH.
OrthoDBiEOG7Q8CXX.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRAFKIKVP ASSANIGPGY DVLGVGLSLF LELDVTIDSS QAQETNDDPN
60 70 80 90 100
NCKLSYTKES EGYSTVPLRS DANLITRTAL YVLRCNNIRN FPSGTKVHVS
110 120 130 140 150
NPIPLGRGLG SSGAAVVAGV ILGNEVAQLG FSKQRMLDYC LMIERHPDNI
160 170 180 190 200
TAAMMGGFCG SFLRDLTPQE VERREIPLAE VLPEPSGGED TGLVPPLPPT
210 220 230 240 250
DIGRHVKYQW NPAIKCIAII PQFELSTADS RGVLPKAYPT QDLVFNLQRL
260 270 280 290 300
AVLTTALTMD PPNADLIYPA MQDRVHQPYR KTLIPGLTEI LSCVTPSTYP
310 320 330 340 350
GLLGICLSGA GPTILALATE NFEEISQEII NRFAKNGIKC SWKLLEPAYD

GASVEQQ
Length:357
Mass (Da):38,712
Last modified:January 23, 2007 - v4
Checksum:i1C1A951E00550ECB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871Missing in CAA37083 (PubMed:2165904).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52901 Genomic DNA. Translation: CAA37083.1.
M37692 Genomic DNA. Translation: AAA35154.1.
U10399 Genomic DNA. Translation: AAB68871.1.
BK006934 Genomic DNA. Translation: DAA06716.1.
PIRiS46772.
RefSeqiNP_011890.1. NM_001179155.1.

Genome annotation databases

EnsemblFungiiYHR025W; YHR025W; YHR025W.
GeneIDi856420.
KEGGisce:YHR025W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52901 Genomic DNA. Translation: CAA37083.1.
M37692 Genomic DNA. Translation: AAA35154.1.
U10399 Genomic DNA. Translation: AAB68871.1.
BK006934 Genomic DNA. Translation: DAA06716.1.
PIRiS46772.
RefSeqiNP_011890.1. NM_001179155.1.

3D structure databases

ProteinModelPortaliP17423.
SMRiP17423. Positions 4-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36456. 104 interactions.
DIPiDIP-4305N.
IntActiP17423. 9 interactions.
MINTiMINT-561221.

PTM databases

iPTMnetiP17423.

Proteomic databases

MaxQBiP17423.
PeptideAtlasiP17423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR025W; YHR025W; YHR025W.
GeneIDi856420.
KEGGisce:YHR025W.

Organism-specific databases

EuPathDBiFungiDB:YHR025W.
SGDiS000001067. THR1.

Phylogenomic databases

HOGENOMiHOG000247199.
InParanoidiP17423.
KOiK00872.
OMAiCANRIPH.
OrthoDBiEOG7Q8CXX.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00064.
BioCyciYEAST:YHR025W-MONOMER.

Miscellaneous databases

NextBioi981988.
PROiP17423.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast homoserine kinase. Characteristics of the corresponding gene, THR1, and the purified enzyme, and evolutionary relationships with other enzymes of threonine metabolism."
    Mannhaupt G., Pohlenz H.D., Seefluth A.K., Pilz U., Feldmann H.
    Eur. J. Biochem. 191:115-122(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.
  2. "Saccharomyces cerevisiae homoserine kinase is homologous to prokaryotic homoserine kinases."
    Schultes N.P., Ellington A.D., Cherry J.M., Szostak J.W.
    Gene 96:177-180(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKHSE_YEAST
AccessioniPrimary (citable) accession number: P17423
Secondary accession number(s): D3DKX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 92600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.