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Protein

Homoserine kinase

Gene

THR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.By similarity

Miscellaneous

Present with 92600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-homoserine = ADP + O-phospho-L-homoserine.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
  4. Homoserine kinase (THR1)
  5. Threonine synthase (THR4)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi104 – 114ATPSequence analysisAdd BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • homoserine kinase activity Source: SGD

GO - Biological processi

  • homoserine metabolic process Source: SGD
  • isoleucine metabolic process Source: SGD
  • methionine biosynthetic process Source: GO_Central
  • methionine metabolic process Source: SGD
  • threonine biosynthetic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Threonine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YHR025W-MONOMER
UniPathwayiUPA00050; UER00064

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine kinase (EC:2.7.1.39)
Short name:
HK
Short name:
HSK
Gene namesi
Name:THR1
Ordered Locus Names:YHR025W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR025W
SGDiS000001067 THR1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001566562 – 357Homoserine kinaseAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP17423
PaxDbiP17423
PRIDEiP17423

PTM databases

iPTMnetiP17423

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
UBE2IP632793EBI-9685,EBI-80168From Homo sapiens.

Protein-protein interaction databases

BioGridi36456, 111 interactors
DIPiDIP-4305N
IntActiP17423, 17 interactors
MINTiP17423
STRINGi4932.YHR025W

Structurei

3D structure databases

ProteinModelPortaliP17423
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000247199
InParanoidiP17423
KOiK00872
OMAiPDNVAPC
OrthoDBiEOG092C3N9W

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00384 Homoser_kinase, 1 hit
InterProiView protein in InterPro
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR006203 GHMP_knse_ATP-bd_CS
IPR000870 Homoserine_kinase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PANTHERiPTHR20861:SF1 PTHR20861:SF1, 1 hit
PfamiView protein in Pfam
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF000676 Homoser_kin, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00191 thrB, 1 hit
PROSITEiView protein in PROSITE
PS00627 GHMP_KINASES_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRAFKIKVP ASSANIGPGY DVLGVGLSLF LELDVTIDSS QAQETNDDPN
60 70 80 90 100
NCKLSYTKES EGYSTVPLRS DANLITRTAL YVLRCNNIRN FPSGTKVHVS
110 120 130 140 150
NPIPLGRGLG SSGAAVVAGV ILGNEVAQLG FSKQRMLDYC LMIERHPDNI
160 170 180 190 200
TAAMMGGFCG SFLRDLTPQE VERREIPLAE VLPEPSGGED TGLVPPLPPT
210 220 230 240 250
DIGRHVKYQW NPAIKCIAII PQFELSTADS RGVLPKAYPT QDLVFNLQRL
260 270 280 290 300
AVLTTALTMD PPNADLIYPA MQDRVHQPYR KTLIPGLTEI LSCVTPSTYP
310 320 330 340 350
GLLGICLSGA GPTILALATE NFEEISQEII NRFAKNGIKC SWKLLEPAYD

GASVEQQ
Length:357
Mass (Da):38,712
Last modified:January 23, 2007 - v4
Checksum:i1C1A951E00550ECB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87Missing in CAA37083 (PubMed:2165904).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52901 Genomic DNA Translation: CAA37083.1
M37692 Genomic DNA Translation: AAA35154.1
U10399 Genomic DNA Translation: AAB68871.1
BK006934 Genomic DNA Translation: DAA06716.1
PIRiS46772
RefSeqiNP_011890.1, NM_001179155.1

Genome annotation databases

EnsemblFungiiYHR025W; YHR025W; YHR025W
GeneIDi856420
KEGGisce:YHR025W

Similar proteinsi

Entry informationi

Entry nameiKHSE_YEAST
AccessioniPrimary (citable) accession number: P17423
Secondary accession number(s): D3DKX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 167 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health