P17423 (KHSE_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	THR1
Ordered Locus Names:	YHR025W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity.
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine.
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.
Subunit structure	Homodimer.
Miscellaneous	Present with 92600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	homoserine metabolic process Inferred from direct assay Ref.1. Source: SGD isoleucine metabolic process Traceable author statement PubMed 8973190. Source: SGD methionine metabolic process Traceable author statement PubMed 8973190. Source: SGD threonine biosynthetic process Inferred from mutant phenotype PubMed 17247984. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 357	356	Homoserine kinase		PRO_0000156656
Regions
	Nucleotide binding	104 – 114	11	ATP Potential
Amino acid modifications
	Modified residue	167	1	Phosphothreonine Ref.6
Experimental info
	Sequence conflict	87	1	Missing in CAA37083. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P17423 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 1C1A951E00550ECB	FASTA	357	38,712
	10 20 30 40 50 60 MVRAFKIKVP ASSANIGPGY DVLGVGLSLF LELDVTIDSS QAQETNDDPN NCKLSYTKES 70 80 90 100 110 120 EGYSTVPLRS DANLITRTAL YVLRCNNIRN FPSGTKVHVS NPIPLGRGLG SSGAAVVAGV 130 140 150 160 170 180 ILGNEVAQLG FSKQRMLDYC LMIERHPDNI TAAMMGGFCG SFLRDLTPQE VERREIPLAE 190 200 210 220 230 240 VLPEPSGGED TGLVPPLPPT DIGRHVKYQW NPAIKCIAII PQFELSTADS RGVLPKAYPT 250 260 270 280 290 300 QDLVFNLQRL AVLTTALTMD PPNADLIYPA MQDRVHQPYR KTLIPGLTEI LSCVTPSTYP 310 320 330 340 350 GLLGICLSGA GPTILALATE NFEEISQEII NRFAKNGIKC SWKLLEPAYD GASVEQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast homoserine kinase. Characteristics of the corresponding gene, THR1, and the purified enzyme, and evolutionary relationships with other enzymes of threonine metabolism." Mannhaupt G., Pohlenz H.D., Seefluth A.K., Pilz U., Feldmann H. Eur. J. Biochem. 191:115-122(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.
[2]	"Saccharomyces cerevisiae homoserine kinase is homologous to prokaryotic homoserine kinases." Schultes N.P., Ellington A.D., Cherry J.M., Szostak J.W. Gene 96:177-180(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-167, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52901 Genomic DNA. Translation: CAA37083.1. M37692 Genomic DNA. Translation: AAA35154.1. U10399 Genomic DNA. Translation: AAB68871.1. BK006934 Genomic DNA. Translation: DAA06716.1.
PIR	S46772.
RefSeq	NP_011890.1. NM_001179155.1.
3D structure databases
ProteinModelPortal	P17423.
SMR	P17423. Positions 4-322.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4305N.
IntAct	P17423. 7 interactions.
MINT	MINT-561221.
STRING	4932.YHR025W.
Proteomic databases
PaxDb	P17423.
PeptideAtlas	P17423.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR025W; YHR025W; YHR025W.
GeneID	856420.
KEGG	sce:YHR025W.
Organism-specific databases
SGD	S000001067. THR1.
Phylogenomic databases
eggNOG	COG0083.
HOGENOM	HOG000247199.
KO	K00872.
OMA	LHEPYRW.
OrthoDB	EOG4B8NNT.
Enzyme and pathway databases
UniPathway	UPA00050; UER00064.
Gene expression databases
Genevestigator	P17423.
GermOnline	YHR025W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
InterPro	IPR006204. GHMP_kinase_N_dom. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981988.

Entry information

Entry name

KHSE_YEAST

Accession

Primary (citable) accession number: P17423
Secondary accession number(s): D3DKX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 129 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program