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Protein

6-phospho-beta-glucosidase

Gene

chbF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl-beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose.1 Publication

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • NAD+
  • Mn2+, Co2+, Ni2+Note: Divalent metal ion. Manganese, cobalt and nickel ions enhance activity whereas magnesium, calcium, strontium and zinc ions do not.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Sitei112Increases basicity of active site TyrBy similarity1
Binding sitei150SubstrateBy similarity1
Metal bindingi172ManganeseBy similarity1
Metal bindingi203ManganeseBy similarity1
Active sitei258Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi5 – 73NADBy similarityAdd BLAST69

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCobalt, Manganese, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:EG10144-MONOMER
MetaCyc:EG10144-MONOMER
BRENDAi3.2.1.86 2026
SABIO-RKP17411

Protein family/group databases

CAZyiGH4 Glycoside Hydrolase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-glucosidase (EC:3.2.1.86)
Alternative name(s):
Cellobiose-6-phosphate hydrolase
Phospho-chitobiase
Gene namesi
Name:chbF
Synonyms:celF, ydjD
Ordered Locus Names:b1734, JW1723
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10144 chbF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001698582 – 4506-phospho-beta-glucosidaseAdd BLAST449

Proteomic databases

PaxDbiP17411
PRIDEiP17411

Expressioni

Inductioni

By N,N'-diacetylchitobiose.

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259132, 399 interactors
IntActiP17411, 4 interactors
STRINGi316385.ECDH10B_1872

Structurei

3D structure databases

ProteinModelPortaliP17411
SMRiP17411
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiENOG4105F8D Bacteria
COG1486 LUCA
HOGENOMiHOG000239811
InParanoidiP17411
KOiK01222
OMAiYYFNREA
PhylomeDBiP17411

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR019802 GlycHydrolase_4_CS
IPR001088 Glyco_hydro_4
IPR022616 Glyco_hydro_4_C
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR32092 PTHR32092, 1 hit
PfamiView protein in Pfam
PF02056 Glyco_hydro_4, 1 hit
PF11975 Glyco_hydro_4C, 1 hit
PRINTSiPR00732 GLHYDRLASE4
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
PROSITEiView protein in PROSITE
PS01324 GLYCOSYL_HYDROL_F4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKLKVVTI GGGSSYTPEL LEGFIKRYHE LPVSELWLVD VEGGKPKLDI
60 70 80 90 100
IFDLCQRMID NAGVPMKLYK TLDRREALKD ADFVTTQLRV GQLPARELDE
110 120 130 140 150
RIPLSHGYLG QETNGAGGLF KGLRTIPVIF DIVKDVEELC PNAWVINFTN
160 170 180 190 200
PAGMVTEAVY RHTGFKRFIG VCNIPIGMKM FIRDVLMLKD SDDLSIDLFG
210 220 230 240 250
LNHMVFIKDV LINGKSRFAE LLDGVASGQL KASSVKNIFD LPFSEGLIRS
260 270 280 290 300
LNLLPCSYLL YYFKQKEMLA IEMGEYYKGG ARAQVVQKVE KQLFELYKNP
310 320 330 340 350
ELKVKPKELE QRGGAYYSDA ACEVINAIYN DKQAEHYVNI PHHGQIDNIP
360 370 380 390 400
ADWAVEMTCK LGRDGATPHP RITHFDDKVM GLIHTIKGFE IAASNAALSG
410 420 430 440 450
EFNDVLLALN LSPLVHSDRD AELLAREMIL AHEKWLPNFA DCIAELKKAH
Length:450
Mass (Da):50,513
Last modified:January 23, 2007 - v4
Checksum:i7A3BB7299E3ECF5B
GO

Sequence cautioni

The sequence CAA37073 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence CAA47257 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence CAA47259 differs from that shown. Reason: Frameshift at several positions.Curated

Mass spectrometryi

Molecular mass is 50386 Da from positions 2 - 450. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA Translation: CAA37073.1 Frameshift.
U00096 Genomic DNA Translation: AAC74804.1
AP009048 Genomic DNA Translation: BAA15515.1
X66725 Genomic DNA Translation: CAA47257.1 Frameshift.
X66725 Genomic DNA Translation: CAA47259.1 Frameshift.
PIRiF64932
RefSeqiNP_416248.1, NC_000913.3
WP_000078765.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74804; AAC74804; b1734
BAA15515; BAA15515; BAA15515
GeneIDi946266
KEGGiecj:JW1723
eco:b1734
PATRICifig|1411691.4.peg.522

Similar proteinsi

Entry informationi

Entry nameiCHBF_ECOLI
AccessioniPrimary (citable) accession number: P17411
Secondary accession number(s): P37795, P78290
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 151 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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