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P17411

- CHBF_ECOLI

UniProt

P17411 - CHBF_ECOLI

Protein

6-phospho-beta-glucosidase

Gene

chbF

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl-beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose.1 Publication

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Cofactori

    NAD.
    Divalent metal ion. Manganese, cobalt and nickel ions enhance activity whereas magnesium, calcium, strontium and zinc ions do not.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961SubstrateBy similarity
    Sitei112 – 1121Increases basicity of active site TyrBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Metal bindingi172 – 1721ManganeseBy similarity
    Metal bindingi203 – 2031ManganeseBy similarity
    Active sitei258 – 2581Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi5 – 7369NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: EcoCyc
    2. identical protein binding Source: EcoCyc
    3. metal ion binding Source: UniProtKB-KW
    4. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. diacetylchitobiose catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Cobalt, Manganese, Metal-binding, NAD, Nickel

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10144-MONOMER.
    ECOL316407:JW1723-MONOMER.
    MetaCyc:EG10144-MONOMER.
    SABIO-RKP17411.

    Protein family/group databases

    CAZyiGH4. Glycoside Hydrolase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-glucosidase (EC:3.2.1.86)
    Alternative name(s):
    Cellobiose-6-phosphate hydrolase
    Phospho-chitobiase
    Gene namesi
    Name:chbF
    Synonyms:celF, ydjD
    Ordered Locus Names:b1734, JW1723
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10144. chbF.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 4504496-phospho-beta-glucosidasePRO_0000169858Add
    BLAST

    Proteomic databases

    PaxDbiP17411.
    PRIDEiP17411.

    Expressioni

    Inductioni

    By N,N'-diacetylchitobiose.

    Gene expression databases

    GenevestigatoriP17411.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    IntActiP17411. 4 interactions.
    STRINGi511145.b1734.

    Structurei

    3D structure databases

    ProteinModelPortaliP17411.
    SMRiP17411. Positions 4-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 4 family.Curated

    Phylogenomic databases

    eggNOGiCOG1486.
    HOGENOMiHOG000239811.
    KOiK01222.
    OMAiPSGHITE.
    OrthoDBiEOG6CP3SG.
    PhylomeDBiP17411.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view]
    PRINTSiPR00732. GLHYDRLASE4.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P17411-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQKLKVVTI GGGSSYTPEL LEGFIKRYHE LPVSELWLVD VEGGKPKLDI    50
    IFDLCQRMID NAGVPMKLYK TLDRREALKD ADFVTTQLRV GQLPARELDE 100
    RIPLSHGYLG QETNGAGGLF KGLRTIPVIF DIVKDVEELC PNAWVINFTN 150
    PAGMVTEAVY RHTGFKRFIG VCNIPIGMKM FIRDVLMLKD SDDLSIDLFG 200
    LNHMVFIKDV LINGKSRFAE LLDGVASGQL KASSVKNIFD LPFSEGLIRS 250
    LNLLPCSYLL YYFKQKEMLA IEMGEYYKGG ARAQVVQKVE KQLFELYKNP 300
    ELKVKPKELE QRGGAYYSDA ACEVINAIYN DKQAEHYVNI PHHGQIDNIP 350
    ADWAVEMTCK LGRDGATPHP RITHFDDKVM GLIHTIKGFE IAASNAALSG 400
    EFNDVLLALN LSPLVHSDRD AELLAREMIL AHEKWLPNFA DCIAELKKAH 450
    Length:450
    Mass (Da):50,513
    Last modified:January 23, 2007 - v4
    Checksum:i7A3BB7299E3ECF5B
    GO

    Sequence cautioni

    The sequence CAA37073.1 differs from that shown. Reason: Frameshift at several positions.
    The sequence CAA47257.1 differs from that shown. Reason: Frameshift at several positions.
    The sequence CAA47259.1 differs from that shown. Reason: Frameshift at several positions.

    Mass spectrometryi

    Molecular mass is 50386 Da from positions 2 - 450. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52890 Genomic DNA. Translation: CAA37073.1. Frameshift.
    U00096 Genomic DNA. Translation: AAC74804.1.
    AP009048 Genomic DNA. Translation: BAA15515.1.
    X66725 Genomic DNA. Translation: CAA47257.1. Frameshift.
    X66725 Genomic DNA. Translation: CAA47259.1. Frameshift.
    PIRiF64932.
    RefSeqiNP_416248.1. NC_000913.3.
    YP_489995.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74804; AAC74804; b1734.
    BAA15515; BAA15515; BAA15515.
    GeneIDi12930138.
    946266.
    KEGGiecj:Y75_p1709.
    eco:b1734.
    PATRICi32118777. VBIEscCol129921_1806.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52890 Genomic DNA. Translation: CAA37073.1 . Frameshift.
    U00096 Genomic DNA. Translation: AAC74804.1 .
    AP009048 Genomic DNA. Translation: BAA15515.1 .
    X66725 Genomic DNA. Translation: CAA47257.1 . Frameshift.
    X66725 Genomic DNA. Translation: CAA47259.1 . Frameshift.
    PIRi F64932.
    RefSeqi NP_416248.1. NC_000913.3.
    YP_489995.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P17411.
    SMRi P17411. Positions 4-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P17411. 4 interactions.
    STRINGi 511145.b1734.

    Protein family/group databases

    CAZyi GH4. Glycoside Hydrolase Family 4.

    Proteomic databases

    PaxDbi P17411.
    PRIDEi P17411.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74804 ; AAC74804 ; b1734 .
    BAA15515 ; BAA15515 ; BAA15515 .
    GeneIDi 12930138.
    946266.
    KEGGi ecj:Y75_p1709.
    eco:b1734.
    PATRICi 32118777. VBIEscCol129921_1806.

    Organism-specific databases

    EchoBASEi EB0142.
    EcoGenei EG10144. chbF.

    Phylogenomic databases

    eggNOGi COG1486.
    HOGENOMi HOG000239811.
    KOi K01222.
    OMAi PSGHITE.
    OrthoDBi EOG6CP3SG.
    PhylomeDBi P17411.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10144-MONOMER.
    ECOL316407:JW1723-MONOMER.
    MetaCyc:EG10144-MONOMER.
    SABIO-RK P17411.

    Miscellaneous databases

    PROi P17411.

    Gene expression databases

    Genevestigatori P17411.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view ]
    PRINTSi PR00732. GLHYDRLASE4.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12."
      Parker L.L., Hall B.G.
      Genetics 124:455-471(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "A luxAB transcriptional fusion to the cryptic celF gene of Escherichia coli displays increased luminescence in the presence of nickel."
      Guzzo A., Dubow M.S.
      Mol. Gen. Genet. 242:455-460(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-450.
      Strain: K12.
    6. "Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: characterization and assignment to the unusual family 4 of glycosylhydrolases."
      Thompson J., Ruvinov S.B., Freedberg D.I., Hall B.G.
      J. Bacteriol. 181:7339-7345(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-31, CHARACTERIZATION, MASS SPECTROMETRY.
    7. "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon."
      Keyhani N.O., Roseman S.
      Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF CHB OPERON.
    8. "The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system."
      Keyhani N.O., Wang L.-X., Lee Y.C., Roseman S.
      J. Biol. Chem. 275:33084-33090(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCHBF_ECOLI
    AccessioniPrimary (citable) accession number: P17411
    Secondary accession number(s): P37795, P78290
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3