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P17411

- CHBF_ECOLI

UniProt

P17411 - CHBF_ECOLI

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Protein
6-phospho-beta-glucosidase
Gene
chbF, celF, ydjD, b1734, JW1723
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl-beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose.1 Publication

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

NAD.
Divalent metal ion. Manganese, cobalt and nickel ions enhance activity whereas magnesium, calcium, strontium and zinc ions do not.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961Substrate By similarity
Sitei112 – 1121Increases basicity of active site Tyr By similarity
Binding sitei150 – 1501Substrate By similarity
Metal bindingi172 – 1721Manganese By similarity
Metal bindingi203 – 2031Manganese By similarity
Active sitei258 – 2581Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi5 – 7369NAD By similarity
Add
BLAST

GO - Molecular functioni

  1. 6-phospho-beta-glucosidase activity Source: EcoCyc
  2. identical protein binding Source: EcoCyc
  3. metal ion binding Source: UniProtKB-KW
  4. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. diacetylchitobiose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Cobalt, Manganese, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BioCyciEcoCyc:EG10144-MONOMER.
ECOL316407:JW1723-MONOMER.
MetaCyc:EG10144-MONOMER.
SABIO-RKP17411.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-glucosidase (EC:3.2.1.86)
Alternative name(s):
Cellobiose-6-phosphate hydrolase
Phospho-chitobiase
Gene namesi
Name:chbF
Synonyms:celF, ydjD
Ordered Locus Names:b1734, JW1723
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10144. chbF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 4504496-phospho-beta-glucosidase
PRO_0000169858Add
BLAST

Proteomic databases

PaxDbiP17411.
PRIDEiP17411.

Expressioni

Inductioni

By N,N'-diacetylchitobiose.

Gene expression databases

GenevestigatoriP17411.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiP17411. 4 interactions.
STRINGi511145.b1734.

Structurei

3D structure databases

ProteinModelPortaliP17411.
SMRiP17411. Positions 4-439.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1486.
HOGENOMiHOG000239811.
KOiK01222.
OMAiPSGHITE.
OrthoDBiEOG6CP3SG.
PhylomeDBiP17411.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17411-1 [UniParc]FASTAAdd to Basket

« Hide

MSQKLKVVTI GGGSSYTPEL LEGFIKRYHE LPVSELWLVD VEGGKPKLDI    50
IFDLCQRMID NAGVPMKLYK TLDRREALKD ADFVTTQLRV GQLPARELDE 100
RIPLSHGYLG QETNGAGGLF KGLRTIPVIF DIVKDVEELC PNAWVINFTN 150
PAGMVTEAVY RHTGFKRFIG VCNIPIGMKM FIRDVLMLKD SDDLSIDLFG 200
LNHMVFIKDV LINGKSRFAE LLDGVASGQL KASSVKNIFD LPFSEGLIRS 250
LNLLPCSYLL YYFKQKEMLA IEMGEYYKGG ARAQVVQKVE KQLFELYKNP 300
ELKVKPKELE QRGGAYYSDA ACEVINAIYN DKQAEHYVNI PHHGQIDNIP 350
ADWAVEMTCK LGRDGATPHP RITHFDDKVM GLIHTIKGFE IAASNAALSG 400
EFNDVLLALN LSPLVHSDRD AELLAREMIL AHEKWLPNFA DCIAELKKAH 450
Length:450
Mass (Da):50,513
Last modified:January 23, 2007 - v4
Checksum:i7A3BB7299E3ECF5B
GO

Sequence cautioni

The sequence CAA37073.1 differs from that shown. Reason: Frameshift at several positions.
The sequence CAA47257.1 differs from that shown. Reason: Frameshift at several positions.
The sequence CAA47259.1 differs from that shown. Reason: Frameshift at several positions.

Mass spectrometryi

Molecular mass is 50386 Da from positions 2 - 450. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52890 Genomic DNA. Translation: CAA37073.1. Frameshift.
U00096 Genomic DNA. Translation: AAC74804.1.
AP009048 Genomic DNA. Translation: BAA15515.1.
X66725 Genomic DNA. Translation: CAA47257.1. Frameshift.
X66725 Genomic DNA. Translation: CAA47259.1. Frameshift.
PIRiF64932.
RefSeqiNP_416248.1. NC_000913.3.
YP_489995.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74804; AAC74804; b1734.
BAA15515; BAA15515; BAA15515.
GeneIDi12930138.
946266.
KEGGiecj:Y75_p1709.
eco:b1734.
PATRICi32118777. VBIEscCol129921_1806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52890 Genomic DNA. Translation: CAA37073.1 . Frameshift.
U00096 Genomic DNA. Translation: AAC74804.1 .
AP009048 Genomic DNA. Translation: BAA15515.1 .
X66725 Genomic DNA. Translation: CAA47257.1 . Frameshift.
X66725 Genomic DNA. Translation: CAA47259.1 . Frameshift.
PIRi F64932.
RefSeqi NP_416248.1. NC_000913.3.
YP_489995.1. NC_007779.1.

3D structure databases

ProteinModelPortali P17411.
SMRi P17411. Positions 4-439.
ModBasei Search...

Protein-protein interaction databases

IntActi P17411. 4 interactions.
STRINGi 511145.b1734.

Protein family/group databases

CAZyi GH4. Glycoside Hydrolase Family 4.

Proteomic databases

PaxDbi P17411.
PRIDEi P17411.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74804 ; AAC74804 ; b1734 .
BAA15515 ; BAA15515 ; BAA15515 .
GeneIDi 12930138.
946266.
KEGGi ecj:Y75_p1709.
eco:b1734.
PATRICi 32118777. VBIEscCol129921_1806.

Organism-specific databases

EchoBASEi EB0142.
EcoGenei EG10144. chbF.

Phylogenomic databases

eggNOGi COG1486.
HOGENOMi HOG000239811.
KOi K01222.
OMAi PSGHITE.
OrthoDBi EOG6CP3SG.
PhylomeDBi P17411.

Enzyme and pathway databases

BioCyci EcoCyc:EG10144-MONOMER.
ECOL316407:JW1723-MONOMER.
MetaCyc:EG10144-MONOMER.
SABIO-RK P17411.

Miscellaneous databases

PROi P17411.

Gene expression databases

Genevestigatori P17411.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view ]
PRINTSi PR00732. GLHYDRLASE4.
SUPFAMi SSF56327. SSF56327. 1 hit.
PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12."
    Parker L.L., Hall B.G.
    Genetics 124:455-471(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A luxAB transcriptional fusion to the cryptic celF gene of Escherichia coli displays increased luminescence in the presence of nickel."
    Guzzo A., Dubow M.S.
    Mol. Gen. Genet. 242:455-460(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-450.
    Strain: K12.
  6. "Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: characterization and assignment to the unusual family 4 of glycosylhydrolases."
    Thompson J., Ruvinov S.B., Freedberg D.I., Hall B.G.
    J. Bacteriol. 181:7339-7345(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-31, CHARACTERIZATION, MASS SPECTROMETRY.
  7. "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon."
    Keyhani N.O., Roseman S.
    Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CHB OPERON.
  8. "The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system."
    Keyhani N.O., Wang L.-X., Lee Y.C., Roseman S.
    J. Biol. Chem. 275:33084-33090(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCHBF_ECOLI
AccessioniPrimary (citable) accession number: P17411
Secondary accession number(s): P37795, P78290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 127 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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