ID   CHBR_ECOLI              Reviewed;         280 AA.
AC   P17410;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   27-MAR-2024, entry version 171.
DE   RecName: Full=HTH-type transcriptional regulator ChbR;
DE   AltName: Full=Chb operon repressor;
GN   Name=chbR; Synonyms=celD; OrderedLocusNames=b1735, JW1724;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2179047; DOI=10.1093/genetics/124.3.455;
RA   Parker L.L., Hall B.G.;
RT   "Characterization and nucleotide sequence of the cryptic cel operon of
RT   Escherichia coli K12.";
RL   Genetics 124:455-471(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   IDENTIFICATION OF CHB OPERON.
RX   PubMed=9405618; DOI=10.1073/pnas.94.26.14367;
RA   Keyhani N.O., Roseman S.;
RT   "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-
RT   diacetylchitobiose, by expressing the cel operon.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997).
RN   [6]
RP   REGULATION OF CHB OPERON, AND SUBUNIT.
RC   STRAIN=K12 / JM101 / ATCC 33876 / DSM 3948 / NCIMB 11926;
RX   PubMed=15066032; DOI=10.1111/j.1365-2958.2004.03986.x;
RA   Plumbridge J., Pellegrini O.;
RT   "Expression of the chitobiose operon of Escherichia coli is regulated by
RT   three transcription factors: NagC, ChbR and CAP.";
RL   Mol. Microbiol. 52:437-449(2004).
RN   [7]
RP   FUNCTION IN CELLOBIOSE UTILIZATION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=18028317; DOI=10.1111/j.1365-2958.2007.05999.x;
RA   Kachroo A.H., Kancherla A.K., Singh N.S., Varshney U., Mahadevan S.;
RT   "Mutations that alter the regulation of the chb operon of Escherichia coli
RT   allow utilization of cellobiose.";
RL   Mol. Microbiol. 66:1382-1395(2007).
CC   -!- FUNCTION: Dual-function repressor/activator of the chbBCARFG operon. In
CC       the absence of the inducing sugar chitobiose, together with NagC,
CC       represses the chbBCARFG operon for the uptake and metabolism of
CC       chitobiose. In association with Crp, and probably in the presence of
CC       chitobiose 6-phosphate, induces the transcription of the chbBCARFG
CC       operon. {ECO:0000269|PubMed:18028317}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:15066032}.
CC   -!- INDUCTION: By N,N'-diacetylchitobiose.
CC   -!- MISCELLANEOUS: Under prolonged growth on cellobiose as the sole carbon
CC       source, spontaneous mutations in chbR and nagC alter the regulation of
CC       the chbBCARFG operon, allowing the uptake and metabolism of cellobiose.
CC       The mutated regulator makes stronger contact with the target DNA
CC       sequence within the chb promoter and has enhanced recognition of
CC       cellobiose 6-phosphate as an inducer.
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DR   EMBL; X52890; CAA37072.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74805.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15516.1; -; Genomic_DNA.
DR   PIR; S10873; S10873.
DR   RefSeq; NP_416249.1; NC_000913.3.
DR   RefSeq; WP_000983647.1; NZ_SSZK01000001.1.
DR   AlphaFoldDB; P17410; -.
DR   SMR; P17410; -.
DR   BioGRID; 4259133; 117.
DR   BioGRID; 850607; 7.
DR   IntAct; P17410; 7.
DR   STRING; 511145.b1735; -.
DR   PaxDb; 511145-b1735; -.
DR   EnsemblBacteria; AAC74805; AAC74805; b1735.
DR   GeneID; 946247; -.
DR   KEGG; ecj:JW1724; -.
DR   KEGG; eco:b1735; -.
DR   PATRIC; fig|511145.12.peg.1807; -.
DR   EchoBASE; EB0141; -.
DR   eggNOG; COG1917; Bacteria.
DR   eggNOG; COG2169; Bacteria.
DR   HOGENOM; CLU_000445_88_19_6; -.
DR   InParanoid; P17410; -.
DR   OMA; IYNKVES; -.
DR   PhylomeDB; P17410; -.
DR   BioCyc; EcoCyc:EG10143-MONOMER; -.
DR   PRO; PR:P17410; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0001046; F:core promoter sequence-specific DNA binding; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:EcoCyc.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:EcoCyc.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR   InterPro; IPR013096; Cupin_2.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   PANTHER; PTHR43280; ARAC-FAMILY TRANSCRIPTIONAL REGULATOR; 1.
DR   PANTHER; PTHR43280:SF12; HTH-TYPE TRANSCRIPTIONAL REGULATOR CHBR; 1.
DR   Pfam; PF07883; Cupin_2; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF51182; RmlC-like cupins; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..280
FT                   /note="HTH-type transcriptional regulator ChbR"
FT                   /id="PRO_0000194506"
FT   DOMAIN          29..81
FT                   /note="Cupin type-2"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          168..274
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        193..214
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        241..264
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   280 AA;  32968 MW;  281152CAEF363B2F CRC64;
     MMQPVINAPE IATAREQQLF NGKNFHVFIY NKTESISGLH QHDYYEFTLV LTGRYFQEIN
     GKRVLLERGD FVFIPLGSHH QSFYEFGATR ILNVGISKRF FEQHYLPLLP YCFVASQVYR
     TNNAFLTYVE TVISSLNFRE TGLEEFVEMV TFYVINRLRH YREEQVIDDV PQWLKSTVEK
     MHDKEQFSES ALENMVALSA KSQEYLTRAT QRYYGKTPMQ IINEIRINFA KKQLEMTNYS
     VTDIAFEAGY SSPSLFIKTF KKLTSFTPKS YRKKLTEFNQ
//