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P17410 (CHBR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HTH-type transcriptional regulator ChbR
Alternative name(s):
Chb operon repressor
Gene names
Name:chbR
Synonyms:celD
Ordered Locus Names:b1735, JW1724
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length280 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presence of chitobiose 6-phosphate, induces the transcription of the chbBCARFG operon. Ref.7

Subunit structure

Homodimer Probable. Ref.6

Induction

By N,N'-diacetylchitobiose. Ref.6

Miscellaneous

Under prolonged growth on cellobiose as the sole carbon source, spontaneous mutations in chbR and nagC alter the regulation of the chbBCARFG operon, allowing the uptake and metabolism of cellobiose. The mutated regulator makes stronger contact with the target DNA sequence within the chb promoter and has enhanced recognition of cellobiose 6-phosphate as an inducer.

Sequence similarities

Contains 1 HTH araC/xylS-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 280280HTH-type transcriptional regulator ChbR
PRO_0000194506

Regions

DNA binding242 – 26120H-T-H motif By similarity

Sequences

Sequence LengthMass (Da)Tools
P17410 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 281152CAEF363B2F

FASTA28032,968
        10         20         30         40         50         60 
MMQPVINAPE IATAREQQLF NGKNFHVFIY NKTESISGLH QHDYYEFTLV LTGRYFQEIN 

        70         80         90        100        110        120 
GKRVLLERGD FVFIPLGSHH QSFYEFGATR ILNVGISKRF FEQHYLPLLP YCFVASQVYR 

       130        140        150        160        170        180 
TNNAFLTYVE TVISSLNFRE TGLEEFVEMV TFYVINRLRH YREEQVIDDV PQWLKSTVEK 

       190        200        210        220        230        240 
MHDKEQFSES ALENMVALSA KSQEYLTRAT QRYYGKTPMQ IINEIRINFA KKQLEMTNYS 

       250        260        270        280 
VTDIAFEAGY SSPSLFIKTF KKLTSFTPKS YRKKLTEFNQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12."
Parker L.L., Hall B.G.
Genetics 124:455-471(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon."
Keyhani N.O., Roseman S.
Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF CHB OPERON.
[6]"Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP."
Plumbridge J., Pellegrini O.
Mol. Microbiol. 52:437-449(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION OF CHB OPERON, SUBUNIT.
Strain: K12 / JM101 / ATCC 33876 / DSM 3948 / NCIMB 11926.
[7]"Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose."
Kachroo A.H., Kancherla A.K., Singh N.S., Varshney U., Mahadevan S.
Mol. Microbiol. 66:1382-1395(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN CELLOBIOSE UTILIZATION.
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52890 Genomic DNA. Translation: CAA37072.1.
U00096 Genomic DNA. Translation: AAC74805.1.
AP009048 Genomic DNA. Translation: BAA15516.1.
PIRS10873.
RefSeqNP_416249.1. NC_000913.3.
YP_489996.1. NC_007779.1.

3D structure databases

ProteinModelPortalP17410.
SMRP17410. Positions 32-95, 174-276.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP17410. 7 interactions.
STRING511145.b1735.

Protein family/group databases

AraC-XylSSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74805; AAC74805; b1735.
BAA15516; BAA15516; BAA15516.
GeneID12930139.
946247.
KEGGecj:Y75_p1710.
eco:b1735.
PATRIC32118779. VBIEscCol129921_1807.

Organism-specific databases

EchoBASEEB0141.
EcoGeneEG10143. chbR.

Phylogenomic databases

eggNOGCOG2207.
HOGENOMHOG000121054.
KOK03490.
OMAMINAPEI.
OrthoDBEOG6KHFZX.
PhylomeDBP17410.
ProtClustDBPRK10296.

Enzyme and pathway databases

BioCycEcoCyc:EG10143-MONOMER.
ECOL316407:JW1724-MONOMER.

Gene expression databases

GenevestigatorP17410.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
2.60.120.10. 1 hit.
InterProIPR013096. Cupin_2.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamPF07883. Cupin_2. 1 hit.
PF00165. HTH_AraC. 2 hits.
[Graphical view]
PRINTSPR00032. HTHARAC.
SMARTSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF51182. SSF51182. 1 hit.
PROSITEPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP17410.

Entry information

Entry nameCHBR_ECOLI
AccessionPrimary (citable) accession number: P17410
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene