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P17410

- CHBR_ECOLI

UniProt

P17410 - CHBR_ECOLI

Protein

HTH-type transcriptional regulator ChbR

Gene

chbR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presence of chitobiose 6-phosphate, induces the transcription of the chbBCARFG operon.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi242 – 26120H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter sequence-specific DNA binding Source: EcoCyc
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: EcoCyc
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10143-MONOMER.
    ECOL316407:JW1724-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    HTH-type transcriptional regulator ChbR
    Alternative name(s):
    Chb operon repressor
    Gene namesi
    Name:chbR
    Synonyms:celD
    Ordered Locus Names:b1735, JW1724
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10143. chbR.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 280280HTH-type transcriptional regulator ChbRPRO_0000194506Add
    BLAST

    Expressioni

    Inductioni

    By N,N'-diacetylchitobiose.

    Gene expression databases

    GenevestigatoriP17410.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiP17410. 7 interactions.
    STRINGi511145.b1735.

    Structurei

    3D structure databases

    ProteinModelPortaliP17410.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2207.
    HOGENOMiHOG000121054.
    KOiK03490.
    OMAiMINAPEI.
    OrthoDBiEOG6KHFZX.
    PhylomeDBiP17410.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR013096. Cupin_2.
    IPR009057. Homeodomain-like.
    IPR018060. HTH_AraC.
    IPR018062. HTH_AraC-typ_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    IPR020449. Tscrpt_reg_HTH_AraC-type.
    [Graphical view]
    PfamiPF07883. Cupin_2. 1 hit.
    PF00165. HTH_AraC. 2 hits.
    [Graphical view]
    PRINTSiPR00032. HTHARAC.
    SMARTiSM00342. HTH_ARAC. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF51182. SSF51182. 1 hit.
    PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
    PS01124. HTH_ARAC_FAMILY_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17410-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMQPVINAPE IATAREQQLF NGKNFHVFIY NKTESISGLH QHDYYEFTLV    50
    LTGRYFQEIN GKRVLLERGD FVFIPLGSHH QSFYEFGATR ILNVGISKRF 100
    FEQHYLPLLP YCFVASQVYR TNNAFLTYVE TVISSLNFRE TGLEEFVEMV 150
    TFYVINRLRH YREEQVIDDV PQWLKSTVEK MHDKEQFSES ALENMVALSA 200
    KSQEYLTRAT QRYYGKTPMQ IINEIRINFA KKQLEMTNYS VTDIAFEAGY 250
    SSPSLFIKTF KKLTSFTPKS YRKKLTEFNQ 280
    Length:280
    Mass (Da):32,968
    Last modified:August 1, 1990 - v1
    Checksum:i281152CAEF363B2F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52890 Genomic DNA. Translation: CAA37072.1.
    U00096 Genomic DNA. Translation: AAC74805.1.
    AP009048 Genomic DNA. Translation: BAA15516.1.
    PIRiS10873.
    RefSeqiNP_416249.1. NC_000913.3.
    YP_489996.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74805; AAC74805; b1735.
    BAA15516; BAA15516; BAA15516.
    GeneIDi12930139.
    946247.
    KEGGiecj:Y75_p1710.
    eco:b1735.
    PATRICi32118779. VBIEscCol129921_1807.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52890 Genomic DNA. Translation: CAA37072.1 .
    U00096 Genomic DNA. Translation: AAC74805.1 .
    AP009048 Genomic DNA. Translation: BAA15516.1 .
    PIRi S10873.
    RefSeqi NP_416249.1. NC_000913.3.
    YP_489996.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P17410.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P17410. 7 interactions.
    STRINGi 511145.b1735.

    Protein family/group databases

    AraC-XylS Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74805 ; AAC74805 ; b1735 .
    BAA15516 ; BAA15516 ; BAA15516 .
    GeneIDi 12930139.
    946247.
    KEGGi ecj:Y75_p1710.
    eco:b1735.
    PATRICi 32118779. VBIEscCol129921_1807.

    Organism-specific databases

    EchoBASEi EB0141.
    EcoGenei EG10143. chbR.

    Phylogenomic databases

    eggNOGi COG2207.
    HOGENOMi HOG000121054.
    KOi K03490.
    OMAi MINAPEI.
    OrthoDBi EOG6KHFZX.
    PhylomeDBi P17410.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10143-MONOMER.
    ECOL316407:JW1724-MONOMER.

    Miscellaneous databases

    PROi P17410.

    Gene expression databases

    Genevestigatori P17410.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR013096. Cupin_2.
    IPR009057. Homeodomain-like.
    IPR018060. HTH_AraC.
    IPR018062. HTH_AraC-typ_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    IPR020449. Tscrpt_reg_HTH_AraC-type.
    [Graphical view ]
    Pfami PF07883. Cupin_2. 1 hit.
    PF00165. HTH_AraC. 2 hits.
    [Graphical view ]
    PRINTSi PR00032. HTHARAC.
    SMARTi SM00342. HTH_ARAC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF51182. SSF51182. 1 hit.
    PROSITEi PS00041. HTH_ARAC_FAMILY_1. 1 hit.
    PS01124. HTH_ARAC_FAMILY_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12."
      Parker L.L., Hall B.G.
      Genetics 124:455-471(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon."
      Keyhani N.O., Roseman S.
      Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF CHB OPERON.
    6. "Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP."
      Plumbridge J., Pellegrini O.
      Mol. Microbiol. 52:437-449(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: REGULATION OF CHB OPERON, SUBUNIT.
      Strain: K12 / JM101 / ATCC 33876 / DSM 3948 / NCIMB 11926.
    7. "Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose."
      Kachroo A.H., Kancherla A.K., Singh N.S., Varshney U., Mahadevan S.
      Mol. Microbiol. 66:1382-1395(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CELLOBIOSE UTILIZATION.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiCHBR_ECOLI
    AccessioniPrimary (citable) accession number: P17410
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Under prolonged growth on cellobiose as the sole carbon source, spontaneous mutations in chbR and nagC alter the regulation of the chbBCARFG operon, allowing the uptake and metabolism of cellobiose. The mutated regulator makes stronger contact with the target DNA sequence within the chb promoter and has enhanced recognition of cellobiose 6-phosphate as an inducer.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3