Reviewed, UniProtKB/Swiss-Prot P17410 (CHBR_ECOLI)

Names and origin

Protein names

Recommended name:
    HTH-type transcriptional regulator chbR
Alternative name(s):
    Chb operon repressor

Gene names

Name:	chbR
Synonyms:	celD
Ordered Locus Names:	b1735, JW1724

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with nagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with crp, and probably in the presence of chitobiose 6-phosphate, induces the transcription of the chbBCARFG operon. Ref.7
Subunit structure	Homodimer Probable.
Induction	By N,N'-diacetylchitobiose.
Miscellaneous	Under prolonged growth on cellobiose as the sole carbon source, spontaneous mutations in chbR and nagC alter the regulation of the chbBCARFG operon, allowing the uptake and metabolism of cellobiose. The mutated regulator makes stronger contact with the target DNA sequence within the chb promoter and has enhanced recognition of cellobiose 6-phosphate as an inducer.
Sequence similarities	Contains 1 HTH araC/xylS-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1125285,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	HTH-type transcriptional regulator chbR		PRO_0000194506
Regions
	DNA binding	242 – 261	20	H-T-H motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17410-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 281152CAEF363B2F	FASTA	280	32,968
	10 20 30 40 50 60 MMQPVINAPE IATAREQQLF NGKNFHVFIY NKTESISGLH QHDYYEFTLV LTGRYFQEIN 70 80 90 100 110 120 GKRVLLERGD FVFIPLGSHH QSFYEFGATR ILNVGISKRF FEQHYLPLLP YCFVASQVYR 130 140 150 160 170 180 TNNAFLTYVE TVISSLNFRE TGLEEFVEMV TFYVINRLRH YREEQVIDDV PQWLKSTVEK 190 200 210 220 230 240 MHDKEQFSES ALENMVALSA KSQEYLTRAT QRYYGKTPMQ IINEIRINFA KKQLEMTNYS 250 260 270 280 VTDIAFEAGY SSPSLFIKTF KKLTSFTPKS YRKKLTEFNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12." Parker L.L., Hall B.G. Genetics 124:455-471(1990) [PubMed: 2179047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon." Keyhani N.O., Roseman S. Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed: 9405618] [Abstract] Cited for: IDENTIFICATION OF CHB OPERON.
[6]	"Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP." Plumbridge J., Pellegrini O. Mol. Microbiol. 52:437-449(2004) [PubMed: 15066032] [Abstract] Cited for: REGULATION OF CHB OPERON, SUBUNIT. Strain: K12 / JM101 / ATCC 33876 / DSM 3948.
[7]	"Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose." Kachroo A.H., Kancherla A.K., Singh N.S., Varshney U., Mahadevan S. Mol. Microbiol. 66:1382-1395(2007) [PubMed: 18028317] [Abstract] Cited for: FUNCTION IN CELLOBIOSE UTILIZATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52890 Genomic DNA. Translation: CAA37072.1. U00096 Genomic DNA. Translation: AAC74805.1. AP009048 Genomic DNA. Translation: BAA15516.1.
PIR	S10873.
RefSeq	AP_002354.1. NP_416249.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P17410. 1 interaction.
Protein family/group databases
AraC-XylS	Search...
Genome annotation databases
GeneID	946247.
GenomeReviews	Gene locus JW1724 in contig AP009048_GR. Gene locus b1735 in contig U00096_GR.
KEGG	ecj:JW1724. eco:b1735.
Organism-specific databases
EchoBASE	EB0141.
EcoGene	EG10143. chbR.
CMR	Search...
Phylogenomic databases
HOGENOM	P17410.
OMA	P17410. LEITNYS.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10143-MON.
Family and domain databases
InterPro	IPR013096. Cupin_2. IPR012287. Homeodomain-rel. IPR000005. HTH_AraC-typ. IPR018062. HTH_AraC-typ_sub_2. IPR018060. HTH_AraC_domain. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit. G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF07883. Cupin_2. 1 hit. PF00165. HTH_AraC. 1 hit. [Graphical view]
SMART	SM00342. HTH_ARAC. 1 hit. [Graphical view]
PROSITE	PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHBR_ECOLI

Accession

Primary (citable) accession number: P17410

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)