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P17410

- CHBR_ECOLI

UniProt

P17410 - CHBR_ECOLI

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Protein

HTH-type transcriptional regulator ChbR

Gene

chbR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presence of chitobiose 6-phosphate, induces the transcription of the chbBCARFG operon.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi242 – 26120H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. core promoter sequence-specific DNA binding Source: EcoCyc
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: EcoCyc
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10143-MONOMER.
ECOL316407:JW1724-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator ChbR
Alternative name(s):
Chb operon repressor
Gene namesi
Name:chbR
Synonyms:celD
Ordered Locus Names:b1735, JW1724
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10143. chbR.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280HTH-type transcriptional regulator ChbRPRO_0000194506Add
BLAST

Expressioni

Inductioni

By N,N'-diacetylchitobiose.

Gene expression databases

GenevestigatoriP17410.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP17410. 7 interactions.
STRINGi511145.b1735.

Structurei

3D structure databases

ProteinModelPortaliP17410.
SMRiP17410. Positions 205-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2207.
HOGENOMiHOG000121054.
InParanoidiP17410.
KOiK03490.
OMAiMINAPEI.
OrthoDBiEOG6KHFZX.
PhylomeDBiP17410.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR013096. Cupin_2.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF07883. Cupin_2. 1 hit.
PF00165. HTH_AraC. 2 hits.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF51182. SSF51182. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17410-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMQPVINAPE IATAREQQLF NGKNFHVFIY NKTESISGLH QHDYYEFTLV
60 70 80 90 100
LTGRYFQEIN GKRVLLERGD FVFIPLGSHH QSFYEFGATR ILNVGISKRF
110 120 130 140 150
FEQHYLPLLP YCFVASQVYR TNNAFLTYVE TVISSLNFRE TGLEEFVEMV
160 170 180 190 200
TFYVINRLRH YREEQVIDDV PQWLKSTVEK MHDKEQFSES ALENMVALSA
210 220 230 240 250
KSQEYLTRAT QRYYGKTPMQ IINEIRINFA KKQLEMTNYS VTDIAFEAGY
260 270 280
SSPSLFIKTF KKLTSFTPKS YRKKLTEFNQ
Length:280
Mass (Da):32,968
Last modified:August 1, 1990 - v1
Checksum:i281152CAEF363B2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37072.1.
U00096 Genomic DNA. Translation: AAC74805.1.
AP009048 Genomic DNA. Translation: BAA15516.1.
PIRiS10873.
RefSeqiNP_416249.1. NC_000913.3.
YP_489996.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74805; AAC74805; b1735.
BAA15516; BAA15516; BAA15516.
GeneIDi12930139.
946247.
KEGGiecj:Y75_p1710.
eco:b1735.
PATRICi32118779. VBIEscCol129921_1807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37072.1 .
U00096 Genomic DNA. Translation: AAC74805.1 .
AP009048 Genomic DNA. Translation: BAA15516.1 .
PIRi S10873.
RefSeqi NP_416249.1. NC_000913.3.
YP_489996.1. NC_007779.1.

3D structure databases

ProteinModelPortali P17410.
SMRi P17410. Positions 205-273.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P17410. 7 interactions.
STRINGi 511145.b1735.

Protein family/group databases

AraC-XylS Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74805 ; AAC74805 ; b1735 .
BAA15516 ; BAA15516 ; BAA15516 .
GeneIDi 12930139.
946247.
KEGGi ecj:Y75_p1710.
eco:b1735.
PATRICi 32118779. VBIEscCol129921_1807.

Organism-specific databases

EchoBASEi EB0141.
EcoGenei EG10143. chbR.

Phylogenomic databases

eggNOGi COG2207.
HOGENOMi HOG000121054.
InParanoidi P17410.
KOi K03490.
OMAi MINAPEI.
OrthoDBi EOG6KHFZX.
PhylomeDBi P17410.

Enzyme and pathway databases

BioCyci EcoCyc:EG10143-MONOMER.
ECOL316407:JW1724-MONOMER.

Miscellaneous databases

PROi P17410.

Gene expression databases

Genevestigatori P17410.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR013096. Cupin_2.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view ]
Pfami PF07883. Cupin_2. 1 hit.
PF00165. HTH_AraC. 2 hits.
[Graphical view ]
PRINTSi PR00032. HTHARAC.
SMARTi SM00342. HTH_ARAC. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF51182. SSF51182. 1 hit.
PROSITEi PS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12."
    Parker L.L., Hall B.G.
    Genetics 124:455-471(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon."
    Keyhani N.O., Roseman S.
    Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CHB OPERON.
  6. "Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP."
    Plumbridge J., Pellegrini O.
    Mol. Microbiol. 52:437-449(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION OF CHB OPERON, SUBUNIT.
    Strain: K12 / JM101 / ATCC 33876 / DSM 3948 / NCIMB 11926.
  7. "Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose."
    Kachroo A.H., Kancherla A.K., Singh N.S., Varshney U., Mahadevan S.
    Mol. Microbiol. 66:1382-1395(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CELLOBIOSE UTILIZATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCHBR_ECOLI
AccessioniPrimary (citable) accession number: P17410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Under prolonged growth on cellobiose as the sole carbon source, spontaneous mutations in chbR and nagC alter the regulation of the chbBCARFG operon, allowing the uptake and metabolism of cellobiose. The mutated regulator makes stronger contact with the target DNA sequence within the chb promoter and has enhanced recognition of cellobiose 6-phosphate as an inducer.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3