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P17377 (RDRP_REOVJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase lambda-3

Short name=Lambda3
EC=2.7.7.48
Alternative name(s):
Lambda3(Pol)
Gene names
Name:L1
OrganismReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2) [Complete proteome]
Taxonomic identifier10885 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length1267 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subcellular location

Virion Potential. Note: Found in the inner capsid (12 copies).

Sequence similarities

Belongs to the reoviridae RNA-directed RNA polymerase family.

Contains 1 RdRp catalytic domain.

Ontologies

Keywords
   Biological processViral RNA replication
   Cellular componentCapsid protein
Virion
   LigandNucleotide-binding
   Molecular functionNucleotidyltransferase
RNA-directed RNA polymerase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processviral genome replication

Inferred from electronic annotation. Source: InterPro

   Cellular_componentviral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12671267RNA-directed RNA polymerase lambda-3
PRO_0000222744

Regions

Domain555 – 792238RdRp catalytic

Sequences

Sequence LengthMass (Da)Tools
P17377 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: B3F74EEBF257B604

FASTA1,267142,289
        10         20         30         40         50         60 
MSSTILTQFG PFIESISGII DQSNDIFEEA AKAFSAFTRS DVYKALDEIP FHDHATVHIP 

        70         80         90        100        110        120 
PTIYSKPSHD SYYYVDALNR VRRRTYQGDD DVYVPNCSIV ELLEPHETLT SYGRLSSAIE 

       130        140        150        160        170        180 
ARAKEGDPQA RIATTYARIS ESQARQIKAP LEKFVLALLV AESGDALYDP ILQKYDDVAD 

       190        200        210        220        230        240 
LTHNCPLWCF REICRHVSGP LPDRAPYLYL SAGVFWLMSP RMTSAIPPLL SDLVNLAILQ 

       250        260        270        280        290        300 
QTAGLDPSLV KMGVQVCLQA AASASYAWYV LKTKSVFPQN TLHSMYETLE GGFCPNLAWL 

       310        320        330        340        350        360 
EPRSDYKFMY MGATPLSGKY ARTAPSNETR ARQLGEKYGL STIIGDLRQR TRQFTKHDFA 

       370        380        390        400        410        420 
SVRYIRDAMA CTSGIFLVRT PTETVLQEYT QSPEIKVPIP QKDWTGPIGE IRVLKDTTSS 

       430        440        450        460        470        480 
IARYLYRTWY LAAARMASQP RTWDPLFQAI MRSQYVTARG GSGAALRESL YAINVSLPDF 

       490        500        510        520        530        540 
KGLPVKAATK IFQAAQLANL PFSHTSVAIL ADTSMGLRNQ VQRRPRSIMP LNVPQQQVSA 

       550        560        570        580        590        600 
PHTLTADYIN YHMNLSTTSG SAVIEKVIPL GVYASSPPNQ SINIDISACD ASITWDFFLS 

       610        620        630        640        650        660 
VIMAAIHEGV ASSSIGKPFM GVPASIVNDE SVVGVRAARP ISGMQNMVQH LSKLYKRGFS 

       670        680        690        700        710        720 
YRVNDSFSPG NDFTHMTTTF PSGSTATSTE HTANNSTMME TFLNVWGPEH TNDPDVLRLM 

       730        740        750        760        770        780 
RSLTIQRNYV CQGDDGLMII DGTTAGKVSS ETIQKMLELI SAYGEEFGWK YDIAYDGTAE 

       790        800        810        820        830        840 
YLKLYFIFGC RIPNLSRHPI VGKERANSSA EEPWPAILDQ VMGIFFNGVH DGLQWQRWIR 

       850        860        870        880        890        900 
YCSSLCCAFS RQRTMIGEEV RYLQYPMWSF VYWGLPPVKL FGSDPWIFSW YMPTGDLGMY 

       910        920        930        940        950        960 
SWISLIRPLM TRWMVANGYV SERCSSIFGN ADYRRCFNDI RLYQGYYMAR LPRNPKKSGR 

       970        980        990       1000       1010       1020 
AAPRETREQF TQALSDYLMQ NPELKSRVLR GRSEWEKYGA GIIHNPPSLF DVPHKWYQGA 

      1030       1040       1050       1060       1070       1080 
QEAATATREE LSDMDDTLLR SRRHTYSSFS KLLEAYLRVR WRMCEAREPS VDLRLPLCAG 

      1090       1100       1110       1120       1130       1140 
IDPLNSDPFL KMVSVGPMLQ STRKYFAQTL FMAKTVSGLD VNAIDSALLR LRTLGADKKA 

      1150       1160       1170       1180       1190       1200 
LTAQLLMVGL QESEADALAG KIMLQDVNTV QLARVVNLAV PDTWMSLDFD SMFKHHVNLL 

      1210       1220       1230       1240       1250       1260 
PKDGRHLNTD IPPRMGWLRA ILRFLGAGVA MTATGVAVNV YLEDIDGGGR SLGQRFMTWM 


RQEGRSA 

« Hide

References

[1]"The sequences of the reovirus serotype 1, 2, and 3 L1 genome segments and analysis of the mode of divergence of the reovirus serotypes."
Wiener J.R., Joklik W.K.
Virology 169:194-203(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31057 Genomic RNA. Translation: AAA47245.1.
PIRMWXR32. B30121.

3D structure databases

ProteinModelPortalP17377.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR012915. RdRP_5.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamPF07925. RdRP_5. 1 hit.
[Graphical view]
PROSITEPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDRP_REOVJ
AccessionPrimary (citable) accession number: P17377
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families