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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:DUT
Ordered Locus Names:VACWR041
ORF Names:F2L
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000344 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182942Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-2156N.
MINTiMINT-130604.

Structurei

3D structure databases

ProteinModelPortaliP17374.
SMRiP17374. Positions 7-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

KOiK01520.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

P17374-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNMNINSPV RFVKETNRAK SPTRQSPYAA GYDLYSAYDY TIPPGERQLI
60 70 80 90 100
KTDISMSMPK FCYGRIAPRS GLSLKGIDIG GGVIDEDYRG NIGVILINNG
110 120 130 140
KCTFNVNTGD RIAQLIYQRI YYPELEEVQS LDSTNRGDQG FGSTGLR
Length:147
Mass (Da):16,405
Last modified:September 5, 2012 - v2
Checksum:i7578ED4F409BF1F2
GO

Sequence cautioni

The sequence AAA48238.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34368 mRNA. Translation: AAA48246.1.
M25392 Genomic DNA. Translation: AAA48238.1. Different initiation.
AY243312 Genomic DNA. Translation: AAO89320.1.
PIRiA32907. PRVZWR.
RefSeqiYP_232923.1. NC_006998.1.

Genome annotation databases

GeneIDi3707498.
KEGGivg:3707498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34368 mRNA. Translation: AAA48246.1.
M25392 Genomic DNA. Translation: AAA48238.1. Different initiation.
AY243312 Genomic DNA. Translation: AAO89320.1.
PIRiA32907. PRVZWR.
RefSeqiYP_232923.1. NC_006998.1.

3D structure databases

ProteinModelPortaliP17374.
SMRiP17374. Positions 7-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-2156N.
MINTiMINT-130604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3707498.
KEGGivg:3707498.

Phylogenomic databases

KOiK01520.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2 kb."
    Roseman N.A., Slabaugh M.B.
    Virology 178:410-418(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Retroviral protease-like gene in the vaccinia virus genome."
    Slabaugh M.B., Roseman N.A.
    Proc. Natl. Acad. Sci. U.S.A. 86:4152-4155(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
    Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDUT_VACCW
AccessioniPrimary (citable) accession number: P17374
Secondary accession number(s): Q80HY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: September 5, 2012
Last modified: April 1, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a protease-like protein (pseudoprotease).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.