P17374 (DUT_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	DUT
Ordered Locus Names:	VACWR041
ORF Names:	F2L

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the dUTPase family.
Caution	Was originally thought to be a protease-like protein (pseudoprotease).
Sequence caution	The sequence AAA48238.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000182942

Sequences

	Sequence		Length	Mass (Da)
	P17374 [UniParc]. Last modified September 5, 2012. Version 2. Checksum: 7578ED4F409BF1F2	FASTA	147	16,405
	10 20 30 40 50 60 MFNMNINSPV RFVKETNRAK SPTRQSPYAA GYDLYSAYDY TIPPGERQLI KTDISMSMPK 70 80 90 100 110 120 FCYGRIAPRS GLSLKGIDIG GGVIDEDYRG NIGVILINNG KCTFNVNTGD RIAQLIYQRI 130 140 YYPELEEVQS LDSTNRGDQG FGSTGLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2 kb." Roseman N.A., Slabaugh M.B. Virology 178:410-418(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Retroviral protease-like gene in the vaccinia virus genome." Slabaugh M.B., Roseman N.A. Proc. Natl. Acad. Sci. U.S.A. 86:4152-4155(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34368 mRNA. Translation: AAA48246.1. M25392 Genomic DNA. Translation: AAA48238.1. Different initiation. AY243312 Genomic DNA. Translation: AAO89320.1.
PIR	PRVZWR. A32907.
RefSeq	YP_232923.1. NC_006998.1.
3D structure databases
ProteinModelPortal	P17374.
SMR	P17374. Positions 7-131.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2156N.
MINT	MINT-130604.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3707498.
Phylogenomic databases
ProtClustDB	PHA3094.
Family and domain databases
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_VACCW

Accession

Primary (citable) accession number: P17374
Secondary accession number(s): Q80HY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	September 5, 2012
Last modified:	April 3, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program