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P17374 (DUT_VACCW) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
Name:DUT
Ordered Locus Names:VACWR041
ORF Names:F2L
OrganismVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]
Taxonomic identifier10254 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Catalytic activity

dUTP + H2O = dUMP + diphosphate.

Cofactor

Magnesium By similarity.

Sequence similarities

Belongs to the dUTPase family.

Caution

Was originally thought to be a protease-like protein (pseudoprotease).

Sequence caution

The sequence AAA48238.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNucleotide metabolism
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdUTP metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functiondUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 147147Deoxyuridine 5'-triphosphate nucleotidohydrolase
PRO_0000182942

Sequences

Sequence LengthMass (Da)Tools
P17374 [UniParc].

Last modified September 5, 2012. Version 2.
Checksum: 7578ED4F409BF1F2

FASTA14716,405
        10         20         30         40         50         60 
MFNMNINSPV RFVKETNRAK SPTRQSPYAA GYDLYSAYDY TIPPGERQLI KTDISMSMPK 

        70         80         90        100        110        120 
FCYGRIAPRS GLSLKGIDIG GGVIDEDYRG NIGVILINNG KCTFNVNTGD RIAQLIYQRI 

       130        140 
YYPELEEVQS LDSTNRGDQG FGSTGLR 

« Hide

References

« Hide 'large scale' references
[1]"The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2 kb."
Roseman N.A., Slabaugh M.B.
Virology 178:410-418(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Retroviral protease-like gene in the vaccinia virus genome."
Slabaugh M.B., Roseman N.A.
Proc. Natl. Acad. Sci. U.S.A. 86:4152-4155(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34368 mRNA. Translation: AAA48246.1.
M25392 Genomic DNA. Translation: AAA48238.1. Different initiation.
AY243312 Genomic DNA. Translation: AAO89320.1.
PIRPRVZWR. A32907.
RefSeqYP_232923.1. NC_006998.1.

3D structure databases

ProteinModelPortalP17374.
SMRP17374. Positions 7-131.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-2156N.
MINTMINT-130604.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3707498.

Phylogenomic databases

ProtClustDBPHA3094.

Family and domain databases

InterProIPR008180. dUTP_pyroPase.
IPR008181. dUTP_pyroPase_sf.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00576. dut. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUT_VACCW
AccessionPrimary (citable) accession number: P17374
Secondary accession number(s): Q80HY0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: September 5, 2012
Last modified: October 16, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families