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P17374

- DUT_VACCW

UniProt

P17374 - DUT_VACCW

Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (05 Sep 2012)
      Previous versions | rss
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    Functioni

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

    Catalytic activityi

    dUTP + H2O = dUMP + diphosphate.

    Cofactori

    Magnesium.By similarity

    GO - Molecular functioni

    1. dUTP diphosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dUTP metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
    Short name:
    dUTPase
    Alternative name(s):
    dUTP pyrophosphatase
    Gene namesi
    Name:DUT
    Ordered Locus Names:VACWR041
    ORF Names:F2L
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000000344: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182942Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-2156N.
    MINTiMINT-130604.

    Structurei

    3D structure databases

    ProteinModelPortaliP17374.
    SMRiP17374. Positions 7-131.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dUTPase family.Curated

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.
    TIGRFAMsiTIGR00576. dut. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P17374-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNMNINSPV RFVKETNRAK SPTRQSPYAA GYDLYSAYDY TIPPGERQLI    50
    KTDISMSMPK FCYGRIAPRS GLSLKGIDIG GGVIDEDYRG NIGVILINNG 100
    KCTFNVNTGD RIAQLIYQRI YYPELEEVQS LDSTNRGDQG FGSTGLR 147
    Length:147
    Mass (Da):16,405
    Last modified:September 5, 2012 - v2
    Checksum:i7578ED4F409BF1F2
    GO

    Sequence cautioni

    The sequence AAA48238.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34368 mRNA. Translation: AAA48246.1.
    M25392 Genomic DNA. Translation: AAA48238.1. Different initiation.
    AY243312 Genomic DNA. Translation: AAO89320.1.
    PIRiA32907. PRVZWR.
    RefSeqiYP_232923.1. NC_006998.1.

    Genome annotation databases

    GeneIDi3707498.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34368 mRNA. Translation: AAA48246.1 .
    M25392 Genomic DNA. Translation: AAA48238.1 . Different initiation.
    AY243312 Genomic DNA. Translation: AAO89320.1 .
    PIRi A32907. PRVZWR.
    RefSeqi YP_232923.1. NC_006998.1.

    3D structure databases

    ProteinModelPortali P17374.
    SMRi P17374. Positions 7-131.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-2156N.
    MINTi MINT-130604.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3707498.

    Family and domain databases

    Gene3Di 2.70.40.10. 1 hit.
    InterProi IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51283. SSF51283. 1 hit.
    TIGRFAMsi TIGR00576. dut. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2 kb."
      Roseman N.A., Slabaugh M.B.
      Virology 178:410-418(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Retroviral protease-like gene in the vaccinia virus genome."
      Slabaugh M.B., Roseman N.A.
      Proc. Natl. Acad. Sci. U.S.A. 86:4152-4155(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
      Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDUT_VACCW
    AccessioniPrimary (citable) accession number: P17374
    Secondary accession number(s): Q80HY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: September 5, 2012
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a protease-like protein (pseudoprotease).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3