Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Protobothrops elegans (Elegant pitviper) (Trimeresurus elegans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom metalloproteinase: impairs hemostasis in the envenomed animal.By similarity
Disintegrin elegantin-1a: inhibits platelet aggregation induced by ADP, thrombin, platelet-activating factor and collagen. Acts by inhibiting fibrinogen interaction with platelet receptors GPIIb/GPIIIa (ITGA2B/ITGB3).1 Publication

Miscellaneous

The sequence shown is that of elegantin-1a.
The disintegrin belongs to the medium disintegrin subfamily.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi333Zinc; catalyticBy similarity1
Active sitei334PROSITE-ProRule annotation1
Metal bindingi337Zinc; catalyticBy similarity1
Metal bindingi343Zinc; catalyticBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 4 chains:
OrganismiProtobothrops elegans (Elegant pitviper) (Trimeresurus elegans)
Taxonomic identifieri88086 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeProtobothrops

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000002898121 – 190By similarityAdd BLAST170
ChainiPRO_0000028982191 – 392Snake venom metalloproteinaseBy similarityAdd BLAST202
PropeptideiPRO_0000028983393 – 4082 PublicationsAdd BLAST16
ChainiPRO_0000028984409 – 481Disintegrin elegantin-1aAdd BLAST73
ChainiPRO_0000028985411 – 481Disintegrin elegantin-1bAdd BLAST71
ChainiPRO_0000028986411 – 480Disintegrin elegantin-1cAdd BLAST70

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi308 ↔ 387By similarity
Disulfide bondi349 ↔ 371By similarity
Disulfide bondi351 ↔ 354By similarity
Disulfide bondi414 ↔ 429By similarity
Disulfide bondi416 ↔ 424By similarity
Disulfide bondi423 ↔ 446By similarity
Disulfide bondi437 ↔ 443By similarity
Disulfide bondi442 ↔ 467By similarity
Disulfide bondi455 ↔ 474By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP17349.
SMRiP17349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 392Peptidase M12BPROSITE-ProRule annotationAdd BLAST196
Domaini400 – 481DisintegrinPROSITE-ProRule annotationAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi459 – 461Cell attachment site3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17349-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQVLLVTIC LAVFPYQGSS IILESGNVDD YEVVYPRKVT ALPKGAVQPK
60 70 80 90 100
YEDAMQYEFK VNGEAVVLHL EKNKGLFSED YSETHYSPDG REITTYPSVE
110 120 130 140 150
DHCYYHGRIH NDADSTASIS ACDGLKGYFK LQGETYLIEP LELSDSEAHA
160 170 180 190 200
VFKYENVEKE DEAPKMCGVT QNWESDESIK KASQLYLTPE QQRFPQRYIK
210 220 230 240 250
LAIVVDHGMY TKYSSNFKKI RKRVHQMVSN INEMCRPLNI AITLALLDVW
260 270 280 290 300
SEKDFITVQA DAPTTAGLFG DWRERVLLKK KNHDHAQLLT DTNFARNTIG
310 320 330 340 350
WAYLGRMCDE KYSVGVVQDH SSKVFMVAVT MTHELGHNLG MEHDDKDKCK
360 370 380 390 400
CEACIMSAVI SDKQSKLFSD CSKDYYQTFL TNDNPQCILN APLRTDTVST
410 420 430 440 450
PVSGNEFLEA GEECDCGSPE NPCCDAATCK LRPGAQCADG LCCDQCRFKK
460 470 480
KRTICRRARG DNPDDRCTGQ SADCPRNGLY S
Length:481
Mass (Da):54,208
Last modified:August 31, 2004 - v2
Checksum:iC5ADDC1E9CE0E526
GO

Mass spectrometryi

Molecular mass is 7880.2±0.8 Da from positions 409 - 481. Determined by ESI. Isoform 1a.1 Publication
Molecular mass is 7678.6±0.4 Da from positions 411 - 481. Determined by ESI. Isoform 1b.1 Publication
Molecular mass is 7591.9±0.4 Da from positions 411 - 480. Determined by ESI. Isoform 1c.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059571 mRNA. Translation: BAB69657.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059571 mRNA. Translation: BAB69657.1.

3D structure databases

ProteinModelPortaliP17349.
SMRiP17349.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVM2E1_PROEL
AccessioniPrimary (citable) accession number: P17349
Secondary accession number(s): Q90YA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 31, 2004
Last modified: March 15, 2017
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.