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Protein

Guanine nucleotide-binding protein subunit beta-1

Gene

gpb-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. In the early embryo, controls the magnitude of the forces acting on centrosomes but is not required for generating asymmetric forces (PubMed:14534135).Curated1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • embryo development ending in birth or egg hatching Source: WormBase
  • establishment of mitotic spindle orientation Source: WormBase
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of locomotion Source: WormBase
  • regulation of oviposition Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-CEL-1296041. Activation of G protein gated Potassium channels.
R-CEL-4086398. Ca2+ pathway.
R-CEL-418555. G alpha (s) signalling events.
R-CEL-418594. G alpha (i) signalling events.
R-CEL-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-CEL-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
SignaLinkiP17343.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-1
Gene namesi
Name:gpb-1
ORF Names:F13D12.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF13D12.7a; CE02186; WBGene00001679; gpb-1.

Subcellular locationi

GO - Cellular componenti

  • heterotrimeric G-protein complex Source: WormBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in one-cell embryo results in hyperactive nuclear and spindle movements from early prophase to metaphase characterized by vigorous rocking of nuclear/centrosome complex and its failure to center properly. Normal centrosome alignment during metaphase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001277121 – 340Guanine nucleotide-binding protein subunit beta-1Add BLAST340

Proteomic databases

EPDiP17343.
PaxDbiP17343.
PeptideAtlasiP17343.
PRIDEiP17343.

PTM databases

iPTMnetiP17343.

Expressioni

Gene expression databases

BgeeiWBGene00001679.
ExpressionAtlasiP17343. differential.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma (By similarity). Interacts with G protein gamma subunits gpc-1 and gpc-2 and with egl-10 and eat-16 (PubMed:11333232). Interacts with goa-1 (in GDP-bound form) (PubMed:12730122).By similarity2 Publications

Protein-protein interaction databases

IntActiP17343. 1 interactor.
STRINGi6239.F13D12.7a.1.

Structurei

3D structure databases

ProteinModelPortaliP17343.
SMRiP17343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati53 – 83WD 1Add BLAST31
Repeati95 – 125WD 2Add BLAST31
Repeati141 – 170WD 3Add BLAST30
Repeati182 – 212WD 4Add BLAST31
Repeati224 – 254WD 5Add BLAST31
Repeati268 – 298WD 6Add BLAST31
Repeati310 – 340WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
InParanoidiP17343.
KOiK04536.
OMAiRIVMRPR.
OrthoDBiEOG091G0A7T.
PhylomeDBiP17343.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELDQLRQE AEQLKSQIRE ARKSANDTTL ATVASNLEPI GRIQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWASDSRNL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGS FVACGGLDNI CSIYSLKTRE GNVRVSRELP GHTGYLSCCR
160 170 180 190 200
FLDDNQIVTS SGDMTCALWD IETGQQCTAF TGHTGDVMSL SLSPDFRTFI
210 220 230 240 250
SGACDASAKL WDIRDGMCKQ TFPGHESDIN AVAFFPSGNA FATGSDDATC
260 270 280 290 300
RLFDIRADQE LAMYSHDNII CGITSVAFSK SGRLLFAGYD DFNCNVWDSM
310 320 330 340
RQERAGVLAG HDNRVSCLGV TEDGMAVCTG SWDSFLKIWN
Length:340
Mass (Da):37,406
Last modified:June 20, 2002 - v2
Checksum:iDF83F9C299F124BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti240A → R in CAA35532 (PubMed:11333232).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17497 Genomic DNA. Translation: CAA35532.1.
AF291846 mRNA. Translation: AAK55963.1.
Z49127 Genomic DNA. Translation: CAA88948.1.
PIRiS09591. RGKWB.
T20830.
RefSeqiNP_001254312.1. NM_001267383.1.
UniGeneiCel.38779.

Genome annotation databases

EnsemblMetazoaiF13D12.7a.1; F13D12.7a.1; WBGene00001679.
F13D12.7a.2; F13D12.7a.2; WBGene00001679.
F13D12.7a.3; F13D12.7a.3; WBGene00001679.
GeneIDi174803.
KEGGicel:CELE_F13D12.7.
UCSCiF13D12.7.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17497 Genomic DNA. Translation: CAA35532.1.
AF291846 mRNA. Translation: AAK55963.1.
Z49127 Genomic DNA. Translation: CAA88948.1.
PIRiS09591. RGKWB.
T20830.
RefSeqiNP_001254312.1. NM_001267383.1.
UniGeneiCel.38779.

3D structure databases

ProteinModelPortaliP17343.
SMRiP17343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP17343. 1 interactor.
STRINGi6239.F13D12.7a.1.

PTM databases

iPTMnetiP17343.

Proteomic databases

EPDiP17343.
PaxDbiP17343.
PeptideAtlasiP17343.
PRIDEiP17343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF13D12.7a.1; F13D12.7a.1; WBGene00001679.
F13D12.7a.2; F13D12.7a.2; WBGene00001679.
F13D12.7a.3; F13D12.7a.3; WBGene00001679.
GeneIDi174803.
KEGGicel:CELE_F13D12.7.
UCSCiF13D12.7.1. c. elegans.

Organism-specific databases

CTDi174803.
WormBaseiF13D12.7a; CE02186; WBGene00001679; gpb-1.

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
InParanoidiP17343.
KOiK04536.
OMAiRIVMRPR.
OrthoDBiEOG091G0A7T.
PhylomeDBiP17343.

Enzyme and pathway databases

ReactomeiR-CEL-1296041. Activation of G protein gated Potassium channels.
R-CEL-4086398. Ca2+ pathway.
R-CEL-418555. G alpha (s) signalling events.
R-CEL-418594. G alpha (i) signalling events.
R-CEL-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-CEL-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
SignaLinkiP17343.

Miscellaneous databases

PROiP17343.

Gene expression databases

BgeeiWBGene00001679.
ExpressionAtlasiP17343. differential.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBB1_CAEEL
AccessioniPrimary (citable) accession number: P17343
Secondary accession number(s): Q19394, Q968T1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: June 20, 2002
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.