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Protein

N,N'-diacetylchitobiose permease IIC component

Gene

chbC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CELB-MONOMER.
ECOL316407:JW1726-MONOMER.
MetaCyc:CELB-MONOMER.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose--glucoside (lac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
N,N'-diacetylchitobiose permease IIC component
Alternative name(s):
EIIC-Chb
PTS system N,N'-diacetylchitobiose-specific EIIC component
Gene namesi
Name:chbC
Synonyms:celB
Ordered Locus Names:b1737, JW1726
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10141. chbC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434CytoplasmicSequence analysisAdd
BLAST
Transmembranei35 – 5521HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini56 – 8530PeriplasmicSequence analysisAdd
BLAST
Transmembranei86 – 10621HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini107 – 1137CytoplasmicSequence analysis
Transmembranei114 – 13421HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini135 – 14612PeriplasmicSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini168 – 18619CytoplasmicSequence analysisAdd
BLAST
Transmembranei187 – 20721HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini208 – 22821PeriplasmicSequence analysisAdd
BLAST
Transmembranei229 – 24921HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini250 – 29950CytoplasmicSequence analysisAdd
BLAST
Transmembranei300 – 32021HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini321 – 34323PeriplasmicSequence analysisAdd
BLAST
Transmembranei344 – 36421HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini365 – 3662CytoplasmicSequence analysis
Transmembranei367 – 38721HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini388 – 40821PeriplasmicSequence analysisAdd
BLAST
Transmembranei409 – 42921HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini430 – 45223CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452N,N'-diacetylchitobiose permease IIC componentPRO_0000186485Add
BLAST

Proteomic databases

PaxDbiP17334.
PRIDEiP17334.

Expressioni

Inductioni

By N,N'-diacetylchitobiose.

Interactioni

Protein-protein interaction databases

BioGridi4260315. 19 interactions.
DIPiDIP-9264N.
IntActiP17334. 1 interaction.
STRINGi511145.b1737.

Structurei

3D structure databases

ProteinModelPortaliP17334.
SMRiP17334. Positions 6-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 426419PTS EIIC type-3PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similaritiesi

Contains 1 PTS EIIC type-3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
HOGENOMiHOG000224102.
InParanoidiP17334.
KOiK02761.
OMAiWAPFVIS.
OrthoDBiEOG62VNMQ.
PhylomeDBiP17334.

Family and domain databases

InterProiIPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004796. PTS_IIC_cello.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
[Graphical view]
PIRSFiPIRSF006351. PTS_EIIC-Cellobiose. 1 hit.
TIGRFAMsiTIGR00359. cello_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
PROSITEiPS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVIASLEK VLLPFAVKIG KQPHVNAIKN GFIRLMPLTL AGAMFVLINN
60 70 80 90 100
VFLSFGEGSF FYSLGIRLDA STIETLNGLK GIGGNVYNGT LGIMSLMAPF
110 120 130 140 150
FIGMALAEER KVDALAAGLL SVAAFMTVTP YSVGEAYAVG ANWLGGANII
160 170 180 190 200
SGIIIGLVVA EMFTFIVRRN WVIKLPDSVP ASVSRSFSAL IPGFIILSVM
210 220 230 240 250
GIIAWALNTW GTNFHQIIMD TISTPLASLG SVVGWAYVIF VPLLWFFGIH
260 270 280 290 300
GALALTALDN GIMTPWALEN IATYQQYGSV EAALAAGKTF HIWAKPMLDS
310 320 330 340 350
FIFLGGSGAT LGLILAIFIA SRRADYRQVA KLALPSGIFQ INEPILFGLP
360 370 380 390 400
IIMNPVMFIP FVLVQPILAA ITLAAYYMGI IPPVTNIAPW TMPTGLGAFF
410 420 430 440 450
NTNGSVAALL VALFNLGIAT LIYLPFVVVA NKAQNAIDKE ESEEDIANAL

KF
Length:452
Mass (Da):48,332
Last modified:November 1, 1997 - v2
Checksum:iEC3CBFEED6231068
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti190 – 20516LIPGF…GIIAW → FNSRLYYSFRDGDYCL in CAA37070 (PubMed:2179047).CuratedAdd
BLAST
Sequence conflicti233 – 2353VGW → WL (PubMed:2179047).Curated
Sequence conflicti240 – 26526FVPLL…GIMTP → LSTALVLRIHAACADRTGQR HYDA (PubMed:2179047).CuratedAdd
BLAST
Sequence conflicti361 – 3666FVLVQP → LYWYNR in CAA37070 (PubMed:2179047).Curated
Sequence conflicti405 – 45248SVAAL…NALKF → TSPHCWSHSSTLASQR in CAA37070 (PubMed:2179047).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37070.1.
U00096 Genomic DNA. Translation: AAC74807.1.
AP009048 Genomic DNA. Translation: BAA15518.1.
PIRiA64933.
S10871.
RefSeqiNP_416251.1. NC_000913.3.
WP_000073041.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74807; AAC74807; b1737.
BAA15518; BAA15518; BAA15518.
GeneIDi945982.
KEGGiecj:JW1726.
eco:b1737.
PATRICi32118783. VBIEscCol129921_1809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37070.1.
U00096 Genomic DNA. Translation: AAC74807.1.
AP009048 Genomic DNA. Translation: BAA15518.1.
PIRiA64933.
S10871.
RefSeqiNP_416251.1. NC_000913.3.
WP_000073041.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP17334.
SMRiP17334. Positions 6-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260315. 19 interactions.
DIPiDIP-9264N.
IntActiP17334. 1 interaction.
STRINGi511145.b1737.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose--glucoside (lac) family.

Proteomic databases

PaxDbiP17334.
PRIDEiP17334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74807; AAC74807; b1737.
BAA15518; BAA15518; BAA15518.
GeneIDi945982.
KEGGiecj:JW1726.
eco:b1737.
PATRICi32118783. VBIEscCol129921_1809.

Organism-specific databases

EchoBASEiEB0139.
EcoGeneiEG10141. chbC.

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
HOGENOMiHOG000224102.
InParanoidiP17334.
KOiK02761.
OMAiWAPFVIS.
OrthoDBiEOG62VNMQ.
PhylomeDBiP17334.

Enzyme and pathway databases

BioCyciEcoCyc:CELB-MONOMER.
ECOL316407:JW1726-MONOMER.
MetaCyc:CELB-MONOMER.

Miscellaneous databases

PROiP17334.

Family and domain databases

InterProiIPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004796. PTS_IIC_cello.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
[Graphical view]
PIRSFiPIRSF006351. PTS_EIIC-Cellobiose. 1 hit.
TIGRFAMsiTIGR00359. cello_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
PROSITEiPS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12."
    Parker L.L., Hall B.G.
    Genetics 124:455-471(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus."
    Reizer J., Reizer A., Saier M.H. Jr.
    Res. Microbiol. 141:1061-1067(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon."
    Keyhani N.O., Roseman S.
    Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CHB OPERON.
  7. "The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system."
    Keyhani N.O., Wang L.-X., Lee Y.C., Roseman S.
    J. Biol. Chem. 275:33084-33090(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPTQC_ECOLI
AccessioniPrimary (citable) accession number: P17334
Secondary accession number(s): P76212, P76907, P77332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.