Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PTS system N,N'-diacetylchitobiose-specific EIIC component

Gene

chbC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913117). Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).1 Publication1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CELB-MONOMER.
ECOL316407:JW1726-MONOMER.
MetaCyc:CELB-MONOMER.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system N,N'-diacetylchitobiose-specific EIIC component1 Publication
Alternative name(s):
EIIC-Chb1 Publication
IIcel1 Publication
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIC component1 Publication
Gene namesi
Name:chbC1 Publication
Synonyms:celB1 Publication
Ordered Locus Names:b1737, JW1726
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10141. chbC.

Subcellular locationi

  • Cell inner membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34CytoplasmicSequence analysisAdd BLAST34
Transmembranei35 – 55HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini56 – 85PeriplasmicSequence analysisAdd BLAST30
Transmembranei86 – 106HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini107 – 113CytoplasmicSequence analysis7
Transmembranei114 – 134HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini135 – 146PeriplasmicSequence analysisAdd BLAST12
Transmembranei147 – 167HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini168 – 186CytoplasmicSequence analysisAdd BLAST19
Transmembranei187 – 207HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini208 – 228PeriplasmicSequence analysisAdd BLAST21
Transmembranei229 – 249HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini250 – 299CytoplasmicSequence analysisAdd BLAST50
Transmembranei300 – 320HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini321 – 343PeriplasmicSequence analysisAdd BLAST23
Transmembranei344 – 364HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini365 – 366CytoplasmicSequence analysis2
Transmembranei367 – 387HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini388 – 408PeriplasmicSequence analysisAdd BLAST21
Transmembranei409 – 429HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini430 – 452CytoplasmicSequence analysisAdd BLAST23

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864851 – 452PTS system N,N'-diacetylchitobiose-specific EIIC componentAdd BLAST452

Proteomic databases

PaxDbiP17334.
PRIDEiP17334.

Expressioni

Inductioni

By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260315. 19 interactors.
DIPiDIP-9264N.
IntActiP17334. 1 interactor.
STRINGi511145.b1737.

Structurei

3D structure databases

ProteinModelPortaliP17334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 426PTS EIIC type-3PROSITE-ProRule annotationAdd BLAST419

Domaini

The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIC type-3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
HOGENOMiHOG000224102.
InParanoidiP17334.
KOiK02761.
OMAiMAIKLPE.
PhylomeDBiP17334.

Family and domain databases

InterProiIPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004796. PTS_IIC_cello.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
[Graphical view]
PIRSFiPIRSF006351. PTS_EIIC-Cellobiose. 1 hit.
TIGRFAMsiTIGR00359. cello_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
PROSITEiPS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVIASLEK VLLPFAVKIG KQPHVNAIKN GFIRLMPLTL AGAMFVLINN
60 70 80 90 100
VFLSFGEGSF FYSLGIRLDA STIETLNGLK GIGGNVYNGT LGIMSLMAPF
110 120 130 140 150
FIGMALAEER KVDALAAGLL SVAAFMTVTP YSVGEAYAVG ANWLGGANII
160 170 180 190 200
SGIIIGLVVA EMFTFIVRRN WVIKLPDSVP ASVSRSFSAL IPGFIILSVM
210 220 230 240 250
GIIAWALNTW GTNFHQIIMD TISTPLASLG SVVGWAYVIF VPLLWFFGIH
260 270 280 290 300
GALALTALDN GIMTPWALEN IATYQQYGSV EAALAAGKTF HIWAKPMLDS
310 320 330 340 350
FIFLGGSGAT LGLILAIFIA SRRADYRQVA KLALPSGIFQ INEPILFGLP
360 370 380 390 400
IIMNPVMFIP FVLVQPILAA ITLAAYYMGI IPPVTNIAPW TMPTGLGAFF
410 420 430 440 450
NTNGSVAALL VALFNLGIAT LIYLPFVVVA NKAQNAIDKE ESEEDIANAL

KF
Length:452
Mass (Da):48,332
Last modified:November 1, 1997 - v2
Checksum:iEC3CBFEED6231068
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti190 – 205LIPGF…GIIAW → FNSRLYYSFRDGDYCL in CAA37070 (PubMed:2179047).CuratedAdd BLAST16
Sequence conflicti233 – 235VGW → WL in CAA37070 (PubMed:2179047).Curated3
Sequence conflicti240 – 265FVPLL…GIMTP → LSTALVLRIHAACADRTGQR HYDA in CAA37070 (PubMed:2179047).CuratedAdd BLAST26
Sequence conflicti361 – 366FVLVQP → LYWYNR in CAA37070 (PubMed:2179047).Curated6
Sequence conflicti405 – 452SVAAL…NALKF → TSPHCWSHSSTLASQR in CAA37070 (PubMed:2179047).CuratedAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37070.1.
U00096 Genomic DNA. Translation: AAC74807.1.
AP009048 Genomic DNA. Translation: BAA15518.1.
PIRiA64933.
S10871.
RefSeqiNP_416251.1. NC_000913.3.
WP_000073041.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74807; AAC74807; b1737.
BAA15518; BAA15518; BAA15518.
GeneIDi945982.
KEGGiecj:JW1726.
eco:b1737.
PATRICi32118783. VBIEscCol129921_1809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA. Translation: CAA37070.1.
U00096 Genomic DNA. Translation: AAC74807.1.
AP009048 Genomic DNA. Translation: BAA15518.1.
PIRiA64933.
S10871.
RefSeqiNP_416251.1. NC_000913.3.
WP_000073041.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP17334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260315. 19 interactors.
DIPiDIP-9264N.
IntActiP17334. 1 interactor.
STRINGi511145.b1737.

Protein family/group databases

TCDBi4.A.3.2.1. the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.

Proteomic databases

PaxDbiP17334.
PRIDEiP17334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74807; AAC74807; b1737.
BAA15518; BAA15518; BAA15518.
GeneIDi945982.
KEGGiecj:JW1726.
eco:b1737.
PATRICi32118783. VBIEscCol129921_1809.

Organism-specific databases

EchoBASEiEB0139.
EcoGeneiEG10141. chbC.

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
HOGENOMiHOG000224102.
InParanoidiP17334.
KOiK02761.
OMAiMAIKLPE.
PhylomeDBiP17334.

Enzyme and pathway databases

BioCyciEcoCyc:CELB-MONOMER.
ECOL316407:JW1726-MONOMER.
MetaCyc:CELB-MONOMER.

Miscellaneous databases

PROiP17334.

Family and domain databases

InterProiIPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004796. PTS_IIC_cello.
[Graphical view]
PfamiPF02378. PTS_EIIC. 1 hit.
[Graphical view]
PIRSFiPIRSF006351. PTS_EIIC-Cellobiose. 1 hit.
TIGRFAMsiTIGR00359. cello_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
PROSITEiPS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTQC_ECOLI
AccessioniPrimary (citable) accession number: P17334
Secondary accession number(s): P76212, P76907, P77332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.