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Protein

Glyceraldehyde-3-phosphate dehydrogenase 4

Gene

gpd-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd-2), Glyceraldehyde-3-phosphate dehydrogenase 4 (gpd-4), Glyceraldehyde-3-phosphate dehydrogenase 3 (gpd-3), Glyceraldehyde-3-phosphate dehydrogenase 1 (gpd-1)
  2. Probable phosphoglycerate kinase (pgk-1)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (ipgm-1)
  4. Enolase (enol-1)
  5. Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADBy similarity
Binding sitei85 – 851NAD; via carbonyl oxygenBy similarity
Active sitei158 – 1581NucleophilePROSITE-ProRule annotation
Sitei185 – 1851Activates thiol group during catalysisBy similarity
Binding sitei188 – 1881Glyceraldehyde 3-phosphateBy similarity
Binding sitei240 – 2401Glyceraldehyde 3-phosphateBy similarity
Binding sitei322 – 3221NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 142NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 4 (EC:1.2.1.12)
Short name:
GAPDH-4
Gene namesi
Name:gpd-4
ORF Names:F33H1.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF33H1.2; CE01568; WBGene00001686; gpd-4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Glyceraldehyde-3-phosphate dehydrogenase 4PRO_0000145513Add
BLAST

Proteomic databases

EPDiP17331.
PaxDbiP17331.
PRIDEiP17331.

PTM databases

iPTMnetiP17331.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi39897. 1 interaction.
DIPiDIP-25637N.
MINTiMINT-1068503.
STRINGi6239.F33H1.2.

Structurei

3D structure databases

ProteinModelPortaliP17331.
SMRiP17331. Positions 2-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1593Glyceraldehyde 3-phosphate bindingBy similarity
Regioni217 – 2182Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
HOGENOMiHOG000071678.
InParanoidiP17331.
KOiK00134.
OMAiMKAASSE.
OrthoDBiEOG7Q5HDF.
PhylomeDBiP17331.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKANVGING FGRIGRLVLR AAVEKDTVQV VAVNDPFITI DYMVYLFKYD
60 70 80 90 100
STHGQFKGTV TYDGDFLIVQ KDGKSSHKIK VFNSKDPAAI AWGSVKADFV
110 120 130 140 150
VESTGVFTTK EKASAHLQGG AKKVIISAPS ADAPMYVVGV NHEKYDASND
160 170 180 190 200
HVISNASCTT NCLAPLAKVI NDNFGIIEGL MTTVHAVTAT QKTVDGPSGK
210 220 230 240 250
LWRDGRGAGQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR VPTPDVSVVD
260 270 280 290 300
LTVRLEKPAS MDDIKKVVKA AADGPMKGIL AYTEDQVVST DFVSDPHSSI
310 320 330 340
FDTGACISLN PNFVKLVSWY DNEYGYSNRV VDLIGYIATR G
Length:341
Mass (Da):36,427
Last modified:August 1, 1990 - v1
Checksum:i0DBED0FFD65D60F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52673 Genomic DNA. Translation: CAA36899.1.
Z48783 Genomic DNA. Translation: CAA88697.1.
PIRiS03912. DEKWG4.
RefSeqiNP_496192.1. NM_063791.4.
UniGeneiCel.21347.

Genome annotation databases

EnsemblMetazoaiF33H1.2; F33H1.2; WBGene00001686.
GeneIDi174578.
KEGGicel:CELE_F33H1.2.
UCSCiF33H1.2.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52673 Genomic DNA. Translation: CAA36899.1.
Z48783 Genomic DNA. Translation: CAA88697.1.
PIRiS03912. DEKWG4.
RefSeqiNP_496192.1. NM_063791.4.
UniGeneiCel.21347.

3D structure databases

ProteinModelPortaliP17331.
SMRiP17331. Positions 2-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39897. 1 interaction.
DIPiDIP-25637N.
MINTiMINT-1068503.
STRINGi6239.F33H1.2.

PTM databases

iPTMnetiP17331.

Proteomic databases

EPDiP17331.
PaxDbiP17331.
PRIDEiP17331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF33H1.2; F33H1.2; WBGene00001686.
GeneIDi174578.
KEGGicel:CELE_F33H1.2.
UCSCiF33H1.2.2. c. elegans.

Organism-specific databases

CTDi174578.
WormBaseiF33H1.2; CE01568; WBGene00001686; gpd-4.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
HOGENOMiHOG000071678.
InParanoidiP17331.
KOiK00134.
OMAiMKAASSE.
OrthoDBiEOG7Q5HDF.
PhylomeDBiP17331.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.
ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.

Miscellaneous databases

NextBioi884636.
PROiP17331.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene family of Caenorhabditis elegans."
    Huang X.Y., Barrios L.A.M., Vonkhorporn P., Honda S., Albertson D.G., Hecht R.M.
    J. Mol. Biol. 206:411-424(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiG3P4_CAEEL
AccessioniPrimary (citable) accession number: P17331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 11, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

There are four nearly identical glyceraldehyde 3-phosphate dehydrogenases in Caenorhabditis elegans.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.