P17330 (G3P3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 3
Short name=GAPDH-3
EC=1.2.1.12

Gene names

Name:	gpd-3
ORF Names:	K10B3.7

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	There are four nearly identical glyceraldehyde 3-phosphate dehydrogenases in Caenorhabditis elegans.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Glyceraldehyde-3-phosphate dehydrogenase 3		PRO_0000145512
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	157 – 159	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	217 – 218	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	158	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	85	1	NAD; via carbonyl oxygen By similarity
	Binding site	188	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	240	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	322	1	NAD By similarity
	Site	185	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17330 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 88938B34676637F6	FASTA	341	36,459
	10 20 30 40 50 60 MTKPSVGING FGRIGRLVLR AAVEKDSVNV VAVNDPFISI DYMVYLFQYD STHGRFKGTV 70 80 90 100 110 120 AHEGDYLLVA KEGKSQHKIK VYNSRDPAEI QWGASGADYV VESTGVFTTI EKANAHLKGG 130 140 150 160 170 180 AKKVIISAPS ADAPMFVVGV NHEKYDHAND HIISNASCTT NCLAPLAKVI NDNFGIIEGL 190 200 210 220 230 240 MTTVHAVTAT QKTVDGPSGK LWRDGRGAGQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR 250 260 270 280 290 300 VPTPDVSVVD LTARLEKPAS LDDIKKVIKA AADGPMKGIL AYTEDQVVST DFVSDTNSSI 310 320 330 340 FDAGASISLN PHFVKLVSWY DNEFGYSNRV VDLISYIATK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene family of Caenorhabditis elegans." Huang X.Y., Barrios L.A.M., Vonkhorporn P., Honda S., Albertson D.G., Hecht R.M. J. Mol. Biol. 206:411-424(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15254 Genomic DNA. Translation: CAA33327.1. FO080552 Genomic DNA. Translation: CCD64599.1.
PIR	DEKWG3. S03914.
RefSeq	NP_508534.3. NM_076133.5.
UniGene	Cel.34171.
3D structure databases
ProteinModelPortal	P17330.
SMR	P17330. Positions 6-340.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26747N.
IntAct	P17330. 4 interactions.
MINT	MINT-116081.
STRING	6239.K10B3.7.2.
Proteomic databases
PaxDb	P17330.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	K10B3.7.1; K10B3.7.1; K10B3.7. K10B3.7.2; K10B3.7.2; K10B3.7.
GeneID	180601.
KEGG	cel:CELE_K10B3.7.
UCSC	K10B3.7.1. c. elegans.
Organism-specific databases
CTD	180601.
WormBase	K10B3.7; CE07370; WBGene00001685; gpd-3.
Phylogenomic databases
eggNOG	COG0057.
GeneTree	ENSGT00690000101860.
HOGENOM	HOG000071678.
InParanoid	P17330.
KO	K00134.
OMA	AGHNIVP.
Enzyme and pathway databases
UniPathway	UPA00109; UER00184.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10836. PTHR10836. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	910066.

Entry information

Entry name

G3P3_CAEEL

Accession

Primary (citable) accession number: P17330

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 29, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project