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P17330 (G3P3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase 3

Short name=GAPDH-3
EC=1.2.1.12
Gene names
Name:gpd-3
ORF Names:K10B3.7
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Miscellaneous

There are four nearly identical glyceraldehyde 3-phosphate dehydrogenases in Caenorhabditis elegans.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglucose metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Glyceraldehyde-3-phosphate dehydrogenase 3
PRO_0000145512

Regions

Nucleotide binding13 – 142NAD By similarity
Region157 – 1593Glyceraldehyde 3-phosphate binding By similarity
Region217 – 2182Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1581Nucleophile By similarity
Binding site351NAD By similarity
Binding site851NAD; via carbonyl oxygen By similarity
Binding site1881Glyceraldehyde 3-phosphate By similarity
Binding site2401Glyceraldehyde 3-phosphate By similarity
Binding site3221NAD By similarity
Site1851Activates thiol group during catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P17330 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 88938B34676637F6

FASTA34136,459
        10         20         30         40         50         60 
MTKPSVGING FGRIGRLVLR AAVEKDSVNV VAVNDPFISI DYMVYLFQYD STHGRFKGTV 

        70         80         90        100        110        120 
AHEGDYLLVA KEGKSQHKIK VYNSRDPAEI QWGASGADYV VESTGVFTTI EKANAHLKGG 

       130        140        150        160        170        180 
AKKVIISAPS ADAPMFVVGV NHEKYDHAND HIISNASCTT NCLAPLAKVI NDNFGIIEGL 

       190        200        210        220        230        240 
MTTVHAVTAT QKTVDGPSGK LWRDGRGAGQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR 

       250        260        270        280        290        300 
VPTPDVSVVD LTARLEKPAS LDDIKKVIKA AADGPMKGIL AYTEDQVVST DFVSDTNSSI 

       310        320        330        340 
FDAGASISLN PHFVKLVSWY DNEFGYSNRV VDLISYIATK A 

« Hide

References

« Hide 'large scale' references
[1]"Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene family of Caenorhabditis elegans."
Huang X.Y., Barrios L.A.M., Vonkhorporn P., Honda S., Albertson D.G., Hecht R.M.
J. Mol. Biol. 206:411-424(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15254 Genomic DNA. Translation: CAA33327.1.
FO080552 Genomic DNA. Translation: CCD64599.1.
PIRDEKWG3. S03914.
RefSeqNP_508534.3. NM_076133.5.
UniGeneCel.34171.

3D structure databases

ProteinModelPortalP17330.
SMRP17330. Positions 2-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid45544. 10 interactions.
DIPDIP-26747N.
IntActP17330. 4 interactions.
MINTMINT-116081.
STRING6239.K10B3.7.2.

Proteomic databases

PaxDbP17330.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaK10B3.7.1; K10B3.7.1; WBGene00001685.
K10B3.7.2; K10B3.7.2; WBGene00001685.
GeneID180601.
KEGGcel:CELE_K10B3.7.
UCSCK10B3.7.1. c. elegans.

Organism-specific databases

CTD180601.
WormBaseK10B3.7; CE07370; WBGene00001685; gpd-3.

Phylogenomic databases

eggNOGCOG0057.
GeneTreeENSGT00720000108591.
HOGENOMHOG000071678.
InParanoidP17330.
KOK00134.
OMAFIDTEYA.
PhylomeDBP17330.

Enzyme and pathway databases

UniPathwayUPA00109; UER00184.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR10836. PTHR10836. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
PRINTSPR00078. G3PDHDRGNASE.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio910066.

Entry information

Entry nameG3P3_CAEEL
AccessionPrimary (citable) accession number: P17330
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase