Reviewed, UniProtKB/Swiss-Prot P17324 (SDHL_YEAST)

Names and origin

Protein names

Recommended name:
    L-serine dehydratase
    EC=4.3.1.17
Alternative name(s):
    L-serine deaminase

Gene names

Name:	SDL1
Synonyms:	SDH1
Ordered Locus Names:	YIL167W/YIL168W
ORF Names:	YI9402.08A/YI9402.08B

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-serine = pyruvate + NH3.
Cofactor	Pyridoxal phosphate.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subcellular location	Cytoplasm.
Miscellaneous	Strain S288c has a natural frameshift in position 127, which disrupts the gene coding for this protein and produces two ORFs.
Sequence similarities	Belongs to the serine/threonine dehydratase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to toxin Inferred from expression pattern. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-serine ammonia-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	L-serine dehydratase		PRO_0000185597
Amino acid modifications
	Modified residue	39	1	N6-(pyridoxal phosphate)lysine By similarity
Natural variations
	Natural variant	5	1	H → Y in strain: S288c.
	Natural variant	15 – 16	2	VF → FL in strain: S288c.
	Natural variant	31	1	I → M in strain: S288c.
	Natural variant	52 – 54	3	QQP → EEA in strain: S288c.
	Natural variant	199	1	H → Q in strain: S288c.

Sequences

	Sequence		Length	Mass (Da)
	P17324-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: D8D1EFFB9AD858E1	FASTA	338	36,824
	10 20 30 40 50 60 MEMTHYEKTP LIRQVFNNGK TNSWFYVKHE ILQPGGSFKS RGIGHLIRKS NQQPLSEGSG 70 80 90 100 110 120 KLAVFSSSGG NAGLAAATAC RSMALNCSVV VPKTTKPRMV KKIQSAGAKV IIHGDHWGEA 130 140 150 160 170 180 DEYLRHKLMA QESQHGSKTL YVHPFDNETI WEGHSTIVDE IIEQLKENDI SLPRVKALVC 190 200 210 220 230 240 SVGGGGLFSG IIKGLDRNHL AEKIPVVAVE TAGCDVLNKS LKKGSPVTLE KLTSVATSLA 250 260 270 280 290 300 SPYIASFAFE SFNKYGCKSV VLSDQDVLAT CLRYADDYNF IVEPACGASL HLCYHPEILE 310 320 330 DILEQKIYED DIVIIIACGG SCMTYEDLVK ASSTLNVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the yeast SDH1 gene encoding a serine dehydratase homolog." Seufert W. Nucleic Acids Res. 18:3653-3653(1990) [PubMed: 2194168] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-127. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52657 Genomic DNA. Translation: CAA36883.1. Z46921 Genomic DNA. Translation: CAA87024.1. Frameshift. Z46921 Genomic DNA. Translation: CAA87025.1. Frameshift. AY558344 Genomic DNA. Translation: AAS56670.1. Frameshift.
PIR	S12731.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P17324. 2 interactions.
Organism-specific databases
CYGD	YIL167w. YIL168w.
SGD	S000001429. SDL1. S000001430. YIL168W.
Enzyme and pathway databases
BRENDA	4.3.1.17. 250.
Gene expression databases
Genevestigator	P17324.
Family and domain databases
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PROSITE	PS00165. DEHYDRATASE_SER_THR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SDHL_YEAST

Accession

Primary (citable) accession number: P17324
Secondary accession number(s): P40443, P40444, Q6Q579

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	October 13, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)