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Protein

Colicin I receptor

Gene

cirA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Not yet known. Postulated to participate in iron transport. Outer membrane receptor for colicins IA and IB.

GO - Molecular functioni

  • colicin transmembrane transporter activity Source: EcoCyc
  • receptor activity Source: InterPro
  • siderophore transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • iron assimilation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:EG10155-MONOMER.
ECOL316407:JW2142-MONOMER.
MetaCyc:EG10155-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.4. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin I receptor
Gene namesi
Name:cirA
Synonyms:cir, feuA
Ordered Locus Names:b2155, JW2142
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10155. cirA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003474426 – 663Colicin I receptorAdd BLAST638

Proteomic databases

EPDiP17315.
PaxDbiP17315.
PRIDEiP17315.

2D gel databases

SWISS-2DPAGEP17315.

Expressioni

Inductioni

By iron and by a cAMP/cAMP receptor protein complex.

Interactioni

Protein-protein interaction databases

BioGridi4260473. 90 interactors.
DIPiDIP-9282N.
IntActiP17315. 7 interactors.
MINTiMINT-4794244.
STRINGi511145.b2155.

Structurei

Secondary structure

1663
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 40Combined sources8
Helixi44 – 46Combined sources3
Beta strandi48 – 54Combined sources7
Helixi56 – 60Combined sources5
Helixi68 – 71Combined sources4
Beta strandi77 – 81Combined sources5
Beta strandi83 – 85Combined sources3
Beta strandi87 – 91Combined sources5
Helixi96 – 98Combined sources3
Beta strandi99 – 103Combined sources5
Turni111 – 113Combined sources3
Helixi120 – 122Combined sources3
Beta strandi123 – 125Combined sources3
Helixi127 – 129Combined sources3
Beta strandi130 – 138Combined sources9
Helixi141 – 144Combined sources4
Turni146 – 149Combined sources4
Beta strandi150 – 157Combined sources8
Beta strandi165 – 177Combined sources13
Beta strandi183 – 195Combined sources13
Turni196 – 198Combined sources3
Beta strandi199 – 210Combined sources12
Beta strandi231 – 243Combined sources13
Beta strandi249 – 263Combined sources15
Beta strandi271 – 284Combined sources14
Beta strandi289 – 302Combined sources14
Beta strandi306 – 308Combined sources3
Beta strandi310 – 325Combined sources16
Helixi326 – 328Combined sources3
Beta strandi330 – 344Combined sources15
Helixi346 – 348Combined sources3
Beta strandi349 – 351Combined sources3
Turni353 – 355Combined sources3
Beta strandi357 – 373Combined sources17
Beta strandi376 – 387Combined sources12
Turni388 – 390Combined sources3
Beta strandi391 – 419Combined sources29
Helixi423 – 426Combined sources4
Beta strandi430 – 434Combined sources5
Helixi435 – 437Combined sources3
Beta strandi439 – 442Combined sources4
Beta strandi450 – 462Combined sources13
Helixi466 – 468Combined sources3
Beta strandi472 – 487Combined sources16
Beta strandi490 – 492Combined sources3
Turni494 – 496Combined sources3
Helixi497 – 499Combined sources3
Beta strandi503 – 508Combined sources6
Beta strandi512 – 519Combined sources8
Beta strandi521 – 538Combined sources18
Beta strandi540 – 556Combined sources17
Helixi558 – 560Combined sources3
Beta strandi563 – 579Combined sources17
Beta strandi582 – 585Combined sources4
Beta strandi587 – 596Combined sources10
Beta strandi612 – 624Combined sources13
Beta strandi627 – 635Combined sources9
Beta strandi654 – 663Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HDFX-ray2.65A26-663[»]
2HDIX-ray2.50A26-663[»]
ProteinModelPortaliP17315.
SMRiP17315.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17315.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi31 – 38TonB box8
Motifi646 – 663TonB C-terminal boxAdd BLAST18

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105D05. Bacteria.
COG4771. LUCA.
HOGENOMiHOG000276828.
InParanoidiP17315.
KOiK16089.
OMAiFQNNVDD.
PhylomeDBiP17315.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRLNPFVRV GLCLSAISCA WPVLAVDDDG ETMVVTASSV EQNLKDAPAS
60 70 80 90 100
ISVITQEDLQ RKPVQNLKDV LKEVPGVQLT NEGDNRKGVS IRGLDSSYTL
110 120 130 140 150
ILVDGKRVNS RNAVFRHNDF DLNWIPVDSI ERIEVVRGPM SSLYGSDALG
160 170 180 190 200
GVVNIITKKI GQKWSGTVTV DTTIQEHRDR GDTYNGQFFT SGPLIDGVLG
210 220 230 240 250
MKAYGSLAKR EKDDPQNSTT TDTGETPRIE GFSSRDGNVE FAWTPNQNHD
260 270 280 290 300
FTAGYGFDRQ DRDSDSLDKN RLERQNYSVS HNGRWDYGTS ELKYYGEKVE
310 320 330 340 350
NKNPGNSSPI TSESNTVDGK YTLPLTAINQ FLTVGGEWRH DKLSDAVNLT
360 370 380 390 400
GGTSSKTSAS QYALFVEDEW RIFEPLALTT GVRMDDHETY GEHWSPRAYL
410 420 430 440 450
VYNATDTVTV KGGWATAFKA PSLLQLSPDW TSNSCRGACK IVGSPDLKPE
460 470 480 490 500
TSESWELGLY YMGEEGWLEG VESSVTVFRN DVKDRISISR TSDVNAAPGY
510 520 530 540 550
QNFVGFETGA NGRRIPVFSY YNVNKARIQG VETELKIPFN DEWKLSINYT
560 570 580 590 600
YNDGRDVSNG ENKPLSDLPF HTANGTLDWK PLALEDWSFY VSGHYTGQKR
610 620 630 640 650
ADSATAKTPG GYTIWNTGAA WQVTKDVKLR AGVLNLGDKD LSRDDYSYNE
660
DGRRYFMAVD YRF
Length:663
Mass (Da):73,896
Last modified:November 1, 1997 - v2
Checksum:i2C68A45D4B5EE414
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97S → D in AAA17054 (PubMed:1315732).Curated1
Sequence conflicti528I → N in AAA75183 (PubMed:2644220).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04229 Genomic DNA. Translation: AAA75183.1.
U00007 Genomic DNA. Translation: AAA60531.1.
U00096 Genomic DNA. Translation: AAC75216.1.
AP009048 Genomic DNA. Translation: BAE76632.1.
M19295 Genomic DNA. Translation: AAA23581.1.
M89774 Genomic DNA. Translation: AAA17054.1.
PIRiB64984. QRECIC.
RefSeqiNP_416660.1. NC_000913.3.
WP_000489247.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75216; AAC75216; b2155.
BAE76632; BAE76632; BAE76632.
GeneIDi949042.
KEGGiecj:JW2142.
eco:b2155.
PATRICi32119657. VBIEscCol129921_2239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04229 Genomic DNA. Translation: AAA75183.1.
U00007 Genomic DNA. Translation: AAA60531.1.
U00096 Genomic DNA. Translation: AAC75216.1.
AP009048 Genomic DNA. Translation: BAE76632.1.
M19295 Genomic DNA. Translation: AAA23581.1.
M89774 Genomic DNA. Translation: AAA17054.1.
PIRiB64984. QRECIC.
RefSeqiNP_416660.1. NC_000913.3.
WP_000489247.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HDFX-ray2.65A26-663[»]
2HDIX-ray2.50A26-663[»]
ProteinModelPortaliP17315.
SMRiP17315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260473. 90 interactors.
DIPiDIP-9282N.
IntActiP17315. 7 interactors.
MINTiMINT-4794244.
STRINGi511145.b2155.

Protein family/group databases

TCDBi1.B.14.1.4. the outer membrane receptor (omr) family.

2D gel databases

SWISS-2DPAGEP17315.

Proteomic databases

EPDiP17315.
PaxDbiP17315.
PRIDEiP17315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75216; AAC75216; b2155.
BAE76632; BAE76632; BAE76632.
GeneIDi949042.
KEGGiecj:JW2142.
eco:b2155.
PATRICi32119657. VBIEscCol129921_2239.

Organism-specific databases

EchoBASEiEB0153.
EcoGeneiEG10155. cirA.

Phylogenomic databases

eggNOGiENOG4105D05. Bacteria.
COG4771. LUCA.
HOGENOMiHOG000276828.
InParanoidiP17315.
KOiK16089.
OMAiFQNNVDD.
PhylomeDBiP17315.

Enzyme and pathway databases

BioCyciEcoCyc:EG10155-MONOMER.
ECOL316407:JW2142-MONOMER.
MetaCyc:EG10155-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP17315.
PROiP17315.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010917. TonB_rcpt_CS.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
PS01156. TONB_DEPENDENT_REC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIRA_ECOLI
AccessioniPrimary (citable) accession number: P17315
Secondary accession number(s): Q2MAS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.