P17314 (IAAC3_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-amylase/trypsin inhibitor CM3 Alternative name(s): Chloroform/methanol-soluble protein CM3
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-amylase/trypsin inhibitor. It could be involved in insect defense mechanisms.
Subunit structure	Subunit of the tetrameric inhibitor.
Subcellular location	Secreted.
Tissue specificity	Developing endosperm.
Post-translational modification	Five disulfide bonds are present Probable, which are essential for the inhibitor activity.
Miscellaneous	CM proteins would be involved in the cooking quality of pasta.
Sequence similarities	Belongs to the protease inhibitor I6 (cereal trypsin/alpha-amylase inhibitor) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Alpha-amylase inhibitor Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.3
	Chain	26 – 168	143	Alpha-amylase/trypsin inhibitor CM3		PRO_0000014346

Sequences

	Sequence		Length	Mass (Da)
	P17314 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: A950FF272E90D23C	FASTA	168	18,221
	10 20 30 40 50 60 MACKSSCSLL LLAAVLLSVL AAASASGSCV PGVAFRTNLL PHCRDYVLQQ TCGTFTPGSK 70 80 90 100 110 120 LPEWMTSASI YSPGKPYLAK LYCCQELAEI SQQCRCEALR YFIALPVPSQ PVDPRSGNVG 130 140 150 160 ESGLIDLPGC PREMQWDFVR LLVAPGQCNL ATIHNVRYCP AVEQPLWI

References

[1]	"Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect alpha-amylase." Garcia-Maroto F., Marana C., Mena M., Garcia-Olmedo F., Carbonero P. Plant Mol. Biol. 14:845-853(1990) [PubMed: 2102861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Chinese Spring. Tissue: Endosperm.
[2]	Gautier M.-F., Alary R., Lullien V., Joudrier P. Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Agathe. Tissue: Seed.
[3]	"N-terminal amino acid sequence of chloroform/methanol-soluble proteins and albumins from endosperm of wheat, barley and related species." Shewry P.R., Lafiandra D., Salcedo G., Aragoncillo C., Garcia-Olmedo F., Lew E.J.-L., Dietler M.D., Kasarda D.D. FEBS Lett. 175:359-363(1984) Cited for: PROTEIN SEQUENCE OF 26-60. Strain: cv. Turgidum. Tissue: Endosperm.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17574 mRNA. Translation: CAA35597.1. X61032 mRNA. Translation: CAA43367.1.
PIR	S10029.
UniGene	Ta.817.
3D structure databases
ProteinModelPortal	P17314.
ModBase	Search...
Protein family/group databases
Allergome	1051. Tri a 30. 8191. Tri a 30.0101.
MEROPS	I06.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P17314.
Family and domain databases
InterPro	IPR006106. Allergen/soft/tryp_amyl_inhib. IPR006105. Allergen/tryp_amyl_inhib_CS. IPR016140. Bifunc_inhib/LTP/seed_store. IPR003612. LTP/seed_store/tryp_amyl_inhib. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00808. AMLASEINHBTR.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
PROSITE	PS00426. CEREAL_TRYP_AMYL_INH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAAC3_WHEAT

Accession

Primary (citable) accession number: P17314

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program