P17313 (VG31_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Capsid assembly protein Gp31

Gene names

Name:

31

Organism

Enterobacteria phage T4 (Bacteriophage T4)

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for proper capsid assembly. In absence of Gp31 the major capsid protein (Gp23) assembles into 'lumps'. Acts as a co-chaperonin with the host groEL protein. Ref.7
Subunit structure	Homoheptamer. Forms a stable complex with groEL in the presence of ATP.

Ontologies

Keywords
Biological process	Capsid assembly
Developmental stage	Early protein
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro viral capsid assembly Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 111

111

Capsid assembly protein Gp31

PRO_0000165018

Secondary structure

1

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111

Helix Strand Turn

Details...

Beta strand

15 – 20

6

Helix

25 – 27

3

Helix

39 – 44

6

Beta strand

45 – 54

10

Beta strand

68 – 72

5

Helix

73 – 75

3

Beta strand

77 – 79

3

Helix

82 – 85

4

Helix

91 – 93

3

Beta strand

98 – 102

5

Helix

103 – 105

3

Sequences

	Sequence		Length	Mass (Da)
	P17313 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: D4F75212CB849EFD	FASTA	111	12,079
	10 20 30 40 50 60 MSEVQQLPIR AVGEYVILVS EPAQAGDEEV TESGLIIGKR VQGEVPELCV VHSVGPDVPE 70 80 90 100 110 GFCEVGDLTS LPVGQIRNVP HPFVALGLKQ PKEIKQKFVT CHYKAIPCLY K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of bacteriophage T4 genes between map positions 128.3-130.3." Prilipov A.G., Mesyanzhinov V.V., Aebi U., Kellenberger E. Nucleic Acids Res. 18:3635-3635(1990) [PubMed: 2362813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D.
[2]	"Cloning, sequence, and expression of the temperature-dependent phage T4 capsid assembly gene 31." Nivinskas R., Black L.W. Gene 73:251-257(1988) [PubMed: 3072258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide sequence of bacteriophage T4 gene 31 region." Raudonikiene A., Nivinskas R. Nucleic Acids Res. 18:4280-4280(1990) [PubMed: 2377483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Gene rIII is the nearest downstream neighbour of bacteriophage T4 gene 31." Raudonikiene A., Nivinskas R. Gene 114:85-90(1992) [PubMed: 1587487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Mutational analysis of the phage T4 morphogenetic 31 gene, whose product interacts with the Escherichia coli GroEL protein." Keppel F., Lipinska B., Ang D., Georgopoulos C. Gene 86:19-25(1990) [PubMed: 2311934] [Abstract] Cited for: MUTAGENESIS.
[7]	"Bacteriophage T4 encodes a co-chaperonin that can substitute for Escherichia coli GroES in protein folding." van der Vies S.M., Gatenby A.A., Georgopoulos C. Nature 368:654-656(1994) [PubMed: 7908418] [Abstract] Cited for: FUNCTION.
[8]	"Structural adaptations in the specialized bacteriophage T4 co-chaperonin Gp31 expand the size of the Anfinsen cage." Hunt J.F., van der Vies S.M., Henry L., Deisenhofer J. Cell 90:361-371(1997) [PubMed: 9244309] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X17657 Genomic DNA. Translation: CAA35651.1.
M37882 Genomic DNA. Translation: AAA32506.1.
X54536 Genomic DNA. Translation: CAA38405.1.
M34502 Genomic DNA. Translation: AAA32510.1.
AF158101 Genomic DNA. Translation: AAD42451.1.

PIR

VHBPP4. JT0488.

RefSeq

NP_049825.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G31	X-ray	2.30	A/B/C/D/E/F/G	1-111	[»]
2CGT	electron microscopy	8.20	O/P/Q/R/S/T/U	1-111	[»]

ProteinModelPortal

P17313.

SMR

P17313. Positions 5-111.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258757.

GenomeReviews

Gene locus T4p215 in contig AF158101_GR.

Phylogenomic databases

ProtClustDB

CLSP2343440.

Family and domain databases

InterPro

IPR020818. Chaperonin_Cpn10.
IPR011032. GroES-like.
IPR016416. Phage_T4_Gp31_GroEL.
[Graphical view]

Gene3D

G3DSA:2.30.33.40. Chaprnin_Cpn10. 1 hit.

Pfam

PF00166. Cpn10. 1 hit.
[Graphical view]

PIRSF

PIRSF004380. Phage_GroES_Gp31. 1 hit.

SUPFAM

SSF50129. GroES_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

VG31_BPT4

Accession

Primary (citable) accession number: P17313

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	September 21, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program