Reviewed, UniProtKB/Swiss-Prot P17296 (MPC2_RALEU)

Names and origin

Protein names

Recommended name:
    Metapyrocatechase 2
      Short name=MPC
    EC=1.13.11.2
Alternative name(s):
    CatO2ase
    Catechol 2,3-dioxygenase II

Gene names

Name:

mcpII

Organism

Ralstonia eutropha (Alcaligenes eutrophus)

Taxonomic identifier

106590 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Catechol + O2 = 2-hydroxymuconate semialdehyde.
Cofactor	Fe2+ ion.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Metapyrocatechase 2		PRO_0000085030
Sites
	Metal binding	170	1	Iron By similarity
	Metal binding	227	1	Iron By similarity
	Metal binding	278	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17296-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: B08D6929FDB92D7B	FASTA	320	35,387
	10 20 30 40 50 60 MDTHRADASQ RSQAPAARPR HAVHSIDHYA LEVPDLAVAE RFLDAFGLTV ARTPECLEVY 70 80 90 100 110 120 AADQRCWARF YEGERKRLAY LSFSCFEGDF AGIRQQLAAS GATLVEDPRY GDESGVWFFD 130 140 150 160 170 180 PDGNLVQVKI GPKTSPSSKS PARLEGAPGG QRGAVVRSQV QRVLPRRLSH VLLFTPSVQR 190 200 210 220 230 240 ALDFYRDALG LRLSDRSDDV IAFTHAPYGS DHHLLALVKS SARGWHHAAW DVADVNEVGQ 250 260 270 280 290 300 GASQMAKAGY TQGWGTGRHV LGSNYFFYVL DPWGSFCEYS ADIDYIPAGQ AWPAGDFAAE 310 320 DSLYQWGPDV PEYFVRNTEA

References

[1]	"Nucleotide sequence of the metapyrocatechase II (catechol 2,3-oxygenase II) gene mpcII from Alcaligenes eutrophus JMP 222." Kabisch M., Fortnagel P. Nucleic Acids Res. 18:5543-5543(1990) [PubMed: 2216726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JMP222.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52415 Genomic DNA. Translation: CAA36666.1.
PIR	DAAL2E. S14691.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.2. 1263.
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR000486. Xdiol_dOase_1_2. [Graphical view]
Pfam	PF00903. Glyoxalase. 2 hits. [Graphical view]
ProDom	PD002334. Gly_diox. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPC2_RALEU

Accession

Primary (citable) accession number: P17296

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)