ID   RS12_THETH              Reviewed;         132 AA.
AC   P17293; Q9EYQ6;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   27-MAR-2024, entry version 155.
DE   RecName: Full=Small ribosomal subunit protein uS12 {ECO:0000305};
DE   AltName: Full=30S ribosomal protein S12;
GN   Name=rpsL; Synonyms=rps12;
OS   Thermus thermophilus.
OC   Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=274;
RN   [1]
RP   PROTEIN SEQUENCE OF 2-8.
RC   STRAIN=VK1;
RX   PubMed=1637860; DOI=10.1016/0300-9084(92)90110-z;
RA   Garber M.B., Agalarov S.C., Eliseikina I.A., Fomenkova N.P., Nikonov S.V.,
RA   Sedelnikova S.E., Shikaeva O.S., Vasiliev D., Zhdanov A.S., Liljas A.,
RA   Svensson L.A.;
RT   "Ribosomal proteins from Thermus thermophilus for structural
RT   investigations.";
RL   Biochimie 74:327-336(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-132, AND STREPTOMYCIN RESISTANT
RP   VARIANTS.
RC   STRAIN=ATCC 43815 / IB-21;
RX   PubMed=11371156; DOI=10.1006/jmbi.2001.4676;
RA   Gregory S.T., Cate J.H.D., Dahlberg A.E.;
RT   "Streptomycin-resistant and streptomycin-dependent mutants of the extreme
RT   thermophile Thermus thermophilus.";
RL   J. Mol. Biol. 309:333-338(2001).
RN   [3]
RP   MASS SPECTROMETRY, AND METHYLTHIOLATION AT ASP-89.
RC   STRAIN=ATCC 43815 / IB-21;
RX   PubMed=16287167; DOI=10.1002/pmic.200402111;
RA   Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.;
RT   "Extending ribosomal protein identifications to unsequenced bacterial
RT   strains using matrix-assisted laser desorption/ionization mass
RT   spectrometry.";
RL   Proteomics 5:4818-4831(2005).
CC   -!- FUNCTION: With S4 and S5 plays an important role in translational
CC       accuracy. {ECO:0000250}.
CC   -!- FUNCTION: Interacts with and stabilizes bases of the 16S rRNA that are
CC       involved in tRNA selection in the A site and with the mRNA backbone.
CC       Located at the interface of the 30S and 50S subunits, it traverses the
CC       body of the 30S subunit contacting proteins on the other side and
CC       probably holding the rRNA structure together. The combined cluster of
CC       proteins S8, S12 and S17 appears to hold together the shoulder and
CC       platform of the 30S subunit (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts proteins S8 and
CC       S17. May interact with IF1 in the 30S initiation complex (By
CC       similarity). {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=14516; Method=MALDI; Note=Strain IB-21.;
CC       Evidence={ECO:0000269|PubMed:16287167};
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family.
CC       {ECO:0000305}.
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DR   EMBL; AF316617; AAG38586.1; -; Genomic_DNA.
DR   PDB; 2OM7; EM; 7.30 A; E=1-132.
DR   PDB; 4V8X; X-ray; 3.35 A; AL/CL=1-132.
DR   PDBsum; 2OM7; -.
DR   PDBsum; 4V8X; -.
DR   AlphaFoldDB; P17293; -.
DR   SMR; P17293; -.
DR   IntAct; P17293; 1.
DR   MINT; P17293; -.
DR   OMA; MHRQGPP; -.
DR   EvolutionaryTrace; P17293; -.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd03368; Ribosomal_S12; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   HAMAP; MF_00403_B; Ribosomal_uS12_B; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR006032; Ribosomal_uS12.
DR   InterPro; IPR005679; Ribosomal_uS12_bac.
DR   NCBIfam; TIGR00981; rpsL_bact; 1.
DR   PANTHER; PTHR11652:SF1; 28S RIBOSOMAL PROTEIN S12, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR11652; 30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER; 1.
DR   Pfam; PF00164; Ribosom_S12_S23; 1.
DR   PIRSF; PIRSF002133; Ribosomal_S12/S23; 1.
DR   PRINTS; PR01034; RIBOSOMALS12.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS00055; RIBOSOMAL_S12; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic resistance; Direct protein sequencing;
KW   Methylation; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW   rRNA-binding; tRNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:1637860"
FT   CHAIN           2..132
FT                   /note="Small ribosomal subunit protein uS12"
FT                   /id="PRO_0000146342"
FT   REGION          106..132
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         89
FT                   /note="3-methylthioaspartic acid"
FT                   /evidence="ECO:0000269|PubMed:16287167"
FT   VARIANT         42
FT                   /note="P -> S (in strain: Isolate HG18; streptomycin
FT                   resistant)"
FT   VARIANT         43
FT                   /note="K -> R (in strain: Isolate HG3; streptomycin
FT                   resistant)"
FT   VARIANT         86
FT                   /note="R -> C (in strain: Isolate HG14; streptomycin
FT                   pseudo-dependent)"
FT   VARIANT         86
FT                   /note="R -> H (in strain: Isolate HG31; streptomycin
FT                   pseudo-dependent)"
FT   VARIANT         88
FT                   /note="K -> E (in strain: Isolate HG19; streptomycin
FT                   resistant)"
FT   VARIANT         88
FT                   /note="K -> R (in strain: Isolate HG1; streptomycin
FT                   resistant)"
FT   VARIANT         91
FT                   /note="P -> L (in strain: Isolate HG11; streptomycin
FT                   dependent)"
FT   HELIX           4..9
FT                   /evidence="ECO:0007829|PDB:4V8X"
FT   STRAND          24..26
FT                   /evidence="ECO:0007829|PDB:4V8X"
FT   STRAND          30..40
FT                   /evidence="ECO:0007829|PDB:4V8X"
FT   STRAND          50..57
FT                   /evidence="ECO:0007829|PDB:4V8X"
FT   STRAND          62..66
FT                   /evidence="ECO:0007829|PDB:4V8X"
FT   STRAND          79..82
FT                   /evidence="ECO:0007829|PDB:4V8X"
FT   TURN            115..118
FT                   /evidence="ECO:0007829|PDB:4V8X"
SQ   SEQUENCE   132 AA;  14599 MW;  9943D095FAD4D9BC CRC64;
     MPTINQLVRK GREKVRKKSK VPALKGAPFR RGVCTVVRTV TPKKPNSALR KVAKVRLTSG
     YEVTAYIPGE GHNLQEHSVV LIRGGRVKDL PGVRYHIVRG VYDAAGVKDR KKSRSKYGTK
     KPKEAAKTAA KK
//