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Protein

30S ribosomal protein S12

Gene

rpsL

Organism
Thermus thermophilus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

With S4 and S5 plays an important role in translational accuracy.By similarity
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding
Biological processAntibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S12
Gene namesi
Name:rpsL
Synonyms:rps12
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001463422 – 13230S ribosomal protein S12Add BLAST131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei893-methylthioaspartic acid1 Publication1

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex (By similarity).By similarity

Protein-protein interaction databases

MINTiP17293

Structurei

Secondary structure

1132
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 9Combined sources6
Beta strandi24 – 26Combined sources3
Beta strandi30 – 40Combined sources11
Beta strandi50 – 57Combined sources8
Beta strandi62 – 66Combined sources5
Beta strandi79 – 82Combined sources4
Turni115 – 118Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TWTmodel-O-[»]
2OM7electron microscopy7.30E1-132[»]
4V8XX-ray3.35AL/CL1-132[»]
ProteinModelPortaliP17293
SMRiP17293
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17293

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108UKE Bacteria
COG0048 LUCA

Family and domain databases

CDDicd03368 Ribosomal_S12, 1 hit
HAMAPiMF_00403_B Ribosomal_S12_B, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR006032 Ribosomal_S12/S23
IPR005679 Ribosomal_S12_bac
PANTHERiPTHR11652 PTHR11652, 1 hit
PfamiView protein in Pfam
PF00164 Ribosom_S12_S23, 1 hit
PIRSFiPIRSF002133 Ribosomal_S12/S23, 1 hit
PRINTSiPR01034 RIBOSOMALS12
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00981 rpsL_bact, 1 hit
PROSITEiView protein in PROSITE
PS00055 RIBOSOMAL_S12, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTINQLVRK GREKVRKKSK VPALKGAPFR RGVCTVVRTV TPKKPNSALR
60 70 80 90 100
KVAKVRLTSG YEVTAYIPGE GHNLQEHSVV LIRGGRVKDL PGVRYHIVRG
110 120 130
VYDAAGVKDR KKSRSKYGTK KPKEAAKTAA KK
Length:132
Mass (Da):14,599
Last modified:January 23, 2007 - v3
Checksum:i9943D095FAD4D9BC
GO

Mass spectrometryi

Molecular mass is 14516 Da from positions 2 - 132. Determined by MALDI. Strain IB-21.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti42P → S in strain: Isolate HG18; streptomycin resistant. 1
Natural varianti43K → R in strain: Isolate HG3; streptomycin resistant. 1
Natural varianti86R → C in strain: Isolate HG14; streptomycin pseudo-dependent. 1
Natural varianti86R → H in strain: Isolate HG31; streptomycin pseudo-dependent. 1
Natural varianti88K → E in strain: Isolate HG19; streptomycin resistant. 1
Natural varianti88K → R in strain: Isolate HG1; streptomycin resistant. 1
Natural varianti91P → L in strain: Isolate HG11; streptomycin dependent. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316617 Genomic DNA Translation: AAG38586.1

Similar proteinsi

Entry informationi

Entry nameiRS12_THETH
AccessioniPrimary (citable) accession number: P17293
Secondary accession number(s): Q9EYQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

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