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P17293

- RS12_THETH

UniProt

P17293 - RS12_THETH

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Protein

30S ribosomal protein S12

Gene

rpsL

Organism
Thermus thermophilus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

With S4 and S5 plays an important role in translational accuracy.By similarity
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).By similarity

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-HAMAP
  2. structural constituent of ribosome Source: InterPro
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. response to antibiotic Source: UniProtKB-KW
  2. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

RNA-binding, rRNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S12
Gene namesi
Name:rpsL
Synonyms:rps12
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

  1. small ribosomal subunit Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 13213130S ribosomal protein S12PRO_0000146342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei89 – 8913-methylthioaspartic acidCurated

Proteomic databases

PRIDEiP17293.

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-270023.
STRINGi262724.TTC1333.

Structurei

Secondary structure

1
132
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 96Combined sources
Beta strandi24 – 263Combined sources
Beta strandi30 – 4011Combined sources
Beta strandi50 – 578Combined sources
Beta strandi62 – 665Combined sources
Beta strandi79 – 824Combined sources
Turni115 – 1184Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TWTmodel-O-[»]
2OM7electron microscopy7.30E1-132[»]
4BYBX-ray3.35L1-132[»]
ProteinModelPortaliP17293.
SMRiP17293. Positions 2-126.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17293.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S12P family.Curated

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00403_B. Ribosomal_S12_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view]
PANTHERiPTHR11652. PTHR11652. 1 hit.
PfamiPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSiPR01034. RIBOSOMALS12.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17293-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTINQLVRK GREKVRKKSK VPALKGAPFR RGVCTVVRTV TPKKPNSALR
60 70 80 90 100
KVAKVRLTSG YEVTAYIPGE GHNLQEHSVV LIRGGRVKDL PGVRYHIVRG
110 120 130
VYDAAGVKDR KKSRSKYGTK KPKEAAKTAA KK
Length:132
Mass (Da):14,599
Last modified:January 23, 2007 - v3
Checksum:i9943D095FAD4D9BC
GO

Mass spectrometryi

Molecular mass is 14516 Da from positions 2 - 132. Determined by MALDI. Strain IB-21.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti42 – 421P → S in strain: Isolate HG18; streptomycin resistant.
Natural varianti43 – 431K → R in strain: Isolate HG3; streptomycin resistant.
Natural varianti86 – 861R → C in strain: Isolate HG14; streptomycin pseudo-dependent.
Natural varianti86 – 861R → H in strain: Isolate HG31; streptomycin pseudo-dependent.
Natural varianti88 – 881K → E in strain: Isolate HG19; streptomycin resistant.
Natural varianti88 – 881K → R in strain: Isolate HG1; streptomycin resistant.
Natural varianti91 – 911P → L in strain: Isolate HG11; streptomycin dependent.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316617 Genomic DNA. Translation: AAG38586.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF316617 Genomic DNA. Translation: AAG38586.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TWT model - O - [» ]
2OM7 electron microscopy 7.30 E 1-132 [» ]
4BYB X-ray 3.35 L 1-132 [» ]
ProteinModelPortali P17293.
SMRi P17293. Positions 2-126.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-270023.
STRINGi 262724.TTC1333.

Proteomic databases

PRIDEi P17293.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P17293.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
HAMAPi MF_00403_B. Ribosomal_S12_B.
InterProi IPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view ]
PANTHERi PTHR11652. PTHR11652. 1 hit.
Pfami PF00164. Ribosom_S12_S23. 1 hit.
[Graphical view ]
PIRSFi PIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSi PR01034. RIBOSOMALS12.
SUPFAMi SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR00981. rpsL_bact. 1 hit.
PROSITEi PS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE OF 2-8.
    Strain: VK1.
  2. "Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilus."
    Gregory S.T., Cate J.H.D., Dahlberg A.E.
    J. Mol. Biol. 309:333-338(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-132, STREPTOMYCIN RESISTANT VARIANTS.
    Strain: ATCC 43815 / IB-21.
  3. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
    Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
    Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
    Strain: ATCC 43815 / IB-21.

Entry informationi

Entry nameiRS12_THETH
AccessioniPrimary (citable) accession number: P17293
Secondary accession number(s): Q9EYQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3