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Protein

Tyrosine 3-monooxygenase

Gene

TH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the physiology of adrenergic neurons.

Catalytic activityi

L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactori

Enzyme regulationi

Phosphorylation leads to an increase in the catalytic activity.

Pathwayi: dopamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dopamine from L-tyrosine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tyrosine 3-monooxygenase (TH)
  2. Aromatic-L-amino-acid decarboxylase (DDC)
This subpathway is part of the pathway dopamine biosynthesis, which is itself part of Catecholamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dopamine from L-tyrosine, the pathway dopamine biosynthesis and in Catecholamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi324 – 3241IronBy similarity
Metal bindingi329 – 3291IronBy similarity
Metal bindingi369 – 3691IronBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Catecholamine biosynthesis, Neurotransmitter biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP17289.
UniPathwayiUPA00747; UER00733.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine 3-monooxygenase (EC:1.14.16.2)
Alternative name(s):
Tyrosine 3-hydroxylase
Short name:
TH
Gene namesi
Name:TH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3713.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 491490Tyrosine 3-monooxygenasePRO_0000205560Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine; by CaMK2By similarity
Modified residuei31 – 311PhosphoserineBy similarity
Modified residuei40 – 401Phosphoserine; by CaMK2 and PKABy similarity
Modified residuei465 – 4651PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17289.
PRIDEiP17289.

PTM databases

iPTMnetiP17289.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000038050.

Chemistry

BindingDBiP17289.

Structurei

3D structure databases

ProteinModelPortaliP17289.
SMRiP17289. Positions 154-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3820. Eukaryota.
COG3186. LUCA.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP17289.
KOiK00501.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR001273. ArAA_hydroxylase.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEiPS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTPNAASPQ AKGFRRAVSE LDAKQAEAIM SPRFVGRRQS LIQDARKERE
60 70 80 90 100
KAEAAASSSE SAEAAAWLER DGEAVLTLLF ALPPTRPPAL TRAIKVFETF
110 120 130 140 150
EAHLHHLETR PAQPLRAGSP PLECFVRCEV PGPVVPALLS ALRRVAEDVR
160 170 180 190 200
AAGESKVLWF PRKVSELDKC HHLVTKFDPD LDLDHPGFSD QAYRQRRKLI
210 220 230 240 250
AEIAFQYKQG DPIPHVEYTA EETATWKEVY STLRGLYPTH ACREHLEAFE
260 270 280 290 300
LLERFCGYRE DRIPQLEDVS RFLKERTGFQ LRPAAGLLSA RDFLASLAFR
310 320 330 340 350
VFQCTQYIRH ASSPMHSPEP ECCHELLGHV PMLADRTFAQ FSQDIGLASL
360 370 380 390 400
GVSDEEIEKL STLYWFTVEF GLCKQNGEVK AYGAGLLSSY GELLHSLSEE
410 420 430 440 450
PEIRAFDPDA AAVQPYQDQT YQPVYFVSES FSDAKDKLRS YASRIQRPFS
460 470 480 490
VKFDPYTLAI DVLDSPHAIR HALDGVQDEM QALAHALNAI S
Length:491
Mass (Da):55,123
Last modified:January 23, 2007 - v5
Checksum:i86707712238F12F2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 684AAWL → GSLV in AAA30798 (PubMed:2899135).Curated
Sequence conflicti73 – 731E → K in AAA30798 (PubMed:2899135).Curated
Sequence conflicti83 – 831P → R in AAA30798 (PubMed:2899135).Curated
Sequence conflicti86 – 861R → K in AAA30798 (PubMed:2899135).Curated
Sequence conflicti284 – 2841A → V in AAA30798 (PubMed:2899135).Curated
Sequence conflicti321 – 3211E → D in AAA30798 (PubMed:2899135).Curated
Sequence conflicti328 – 3303GHV → AHG in AAA30798 (PubMed:2899135).Curated
Sequence conflicti380 – 3801K → N in AAA30798 (PubMed:2899135).Curated
Sequence conflicti471 – 4711H → R in AAA30798 (PubMed:2899135).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36794 mRNA. Translation: AAA30779.1.
M36705 mRNA. Translation: AAA30798.1.
PIRiI45983.
JL0039.
RefSeqiNP_776309.1. NM_173884.2.
UniGeneiBt.64937.

Genome annotation databases

GeneIDi280707.
KEGGibta:280707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36794 mRNA. Translation: AAA30779.1.
M36705 mRNA. Translation: AAA30798.1.
PIRiI45983.
JL0039.
RefSeqiNP_776309.1. NM_173884.2.
UniGeneiBt.64937.

3D structure databases

ProteinModelPortaliP17289.
SMRiP17289. Positions 154-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000038050.

Chemistry

BindingDBiP17289.
ChEMBLiCHEMBL3713.

PTM databases

iPTMnetiP17289.

Proteomic databases

PaxDbiP17289.
PRIDEiP17289.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi280707.
KEGGibta:280707.

Organism-specific databases

CTDi7054.

Phylogenomic databases

eggNOGiKOG3820. Eukaryota.
COG3186. LUCA.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP17289.
KOiK00501.

Enzyme and pathway databases

UniPathwayiUPA00747; UER00733.
SABIO-RKP17289.

Miscellaneous databases

PROiP17289.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR001273. ArAA_hydroxylase.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEiPS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTY3H_BOVIN
AccessioniPrimary (citable) accession number: P17289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 128 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.