Reviewed, UniProtKB/Swiss-Prot P17289 (TY3H_BOVIN)

Names and origin

Protein names

Recommended name:
    Tyrosine 3-monooxygenase
    EC=1.14.16.2
Alternative name(s):
    Tyrosine 3-hydroxylase
      Short name=TH

Gene names

Name:

TH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays an important role in the physiology of adrenergic neurons.
Catalytic activity	L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Enzyme regulation	Phosphorylation leads to an increase in the catalytic activity.
Pathway	Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Catecholamine biosynthesis Neurotransmitter biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro catecholamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 491	490	Tyrosine 3-monooxygenase		PRO_0000205560
Sites
	Metal binding	324	1	Iron By similarity
	Metal binding	329	1	Iron By similarity
	Metal binding	369	1	Iron By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	40	1	Phosphoserine; by PKA
Experimental info
	Sequence conflict	65 – 68	4	AAWL → GSLV in AAA30798. Ref.2
	Sequence conflict	73	1	E → K in AAA30798. Ref.2
	Sequence conflict	83	1	P → R in AAA30798. Ref.2
	Sequence conflict	86	1	R → K in AAA30798. Ref.2
	Sequence conflict	284	1	A → V in AAA30798. Ref.2
	Sequence conflict	321	1	E → D in AAA30798. Ref.2
	Sequence conflict	328 – 330	3	GHV → AHG in AAA30798. Ref.2
	Sequence conflict	380	1	K → N in AAA30798. Ref.2
	Sequence conflict	471	1	H → R in AAA30798. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P17289-1 [UniParc]. Last modified January 23, 2007. Version 5. Checksum: 86707712238F12F2	FASTA	491	55,123
	10 20 30 40 50 60 MPTPNAASPQ AKGFRRAVSE LDAKQAEAIM SPRFVGRRQS LIQDARKERE KAEAAASSSE 70 80 90 100 110 120 SAEAAAWLER DGEAVLTLLF ALPPTRPPAL TRAIKVFETF EAHLHHLETR PAQPLRAGSP 130 140 150 160 170 180 PLECFVRCEV PGPVVPALLS ALRRVAEDVR AAGESKVLWF PRKVSELDKC HHLVTKFDPD 190 200 210 220 230 240 LDLDHPGFSD QAYRQRRKLI AEIAFQYKQG DPIPHVEYTA EETATWKEVY STLRGLYPTH 250 260 270 280 290 300 ACREHLEAFE LLERFCGYRE DRIPQLEDVS RFLKERTGFQ LRPAAGLLSA RDFLASLAFR 310 320 330 340 350 360 VFQCTQYIRH ASSPMHSPEP ECCHELLGHV PMLADRTFAQ FSQDIGLASL GVSDEEIEKL 370 380 390 400 410 420 STLYWFTVEF GLCKQNGEVK AYGAGLLSSY GELLHSLSEE PEIRAFDPDA AAVQPYQDQT 430 440 450 460 470 480 YQPVYFVSES FSDAKDKLRS YASRIQRPFS VKFDPYTLAI DVLDSPHAIR HALDGVQDEM 490 QALAHALNAI S

References

[1]	"Isolation and nucleotide sequence of a cDNA clone encoding bovine adrenal tyrosine hydroxylase: comparative analysis of tyrosine hydroxylase gene products." D'Mello S.R., Weisberg E.P., Stachowiak M.K., Turzai L.M., Gioio A.E., Kaplan B.B. J. Neurosci. Res. 19:440-449(1988) [PubMed: 2898537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Predicted amino acid sequence of bovine tyrosine hydroxylase and its similarity to tyrosine hydroxylases from other species." Saadat S., Stehle A.D., Lamouroux A., Mallet J., Thoenen H. J. Neurochem. 51:572-578(1988) [PubMed: 2899135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Characterization of the catalytic domain of bovine adrenal tyrosine hydroxylase." Abate C., Smith J.A., Joh T.H. Biochem. Biophys. Res. Commun. 151:1446-1453(1988) [PubMed: 2895648] [Abstract] Cited for: PROTEIN SEQUENCE OF 154-170.
[4]	"Soluble tyrosine hydroxylase (tyrosine 3-monooxygenase) from bovine adrenal medulla: large-scale purification and physicochemical properties." Haavik J., Andersson K.K., Petersson L., Flatmark T. Biochim. Biophys. Acta 953:142-156(1988) [PubMed: 2894860] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-28. Tissue: Adrenal medulla.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M36794 mRNA. Translation: AAA30779.1. M36705 mRNA. Translation: AAA30798.1.
IPI	IPI00697099.
PIR	I45983. JL0039.
RefSeq	NP_776309.1.
UniGene	Bt.64937
3D structure databases
HSSP	HSSP built from PDB template 1TOH based on UniProtKB P04177.
SMR	P17289. Positions 154-491.
ModBase	Search...
Protein-protein interaction databases
STRING	P17289.
Genome annotation databases
Ensembl	ENSBTAT00000038235; ENSBTAP00000038050; ENSBTAG00000026768; Bos taurus. [Genome view]
GeneID	280707.
KEGG	bta:280707.
Organism-specific databases
CTD	280707.
Phylogenomic databases
HOVERGEN	P17289.
Enzyme and pathway databases
BRENDA	1.14.16.2. 251.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005962. Tyr_3_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01269. Tyr_3_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TY3H_BOVIN

Accession

Primary (citable) accession number: P17289

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 84 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)