P17282 (GAG_SIVCZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gag polyprotein

Alternative name(s):
Pr55Gag

Cleaved into the following 6 chains:

1. Matrix protein p17
   Short name=MA
2. Capsid protein p24
   Short name=CA
3. Spacer peptide p2
4. Nucleocapsid protein p7
   Short name=NC
5. Spacer peptide p1
6. p6-gag

Gene names

Name:

gag

Organism

Simian immunodeficiency virus (isolate CPZ GAB1) (SIV-cpz) (Chimpanzee immunodeficiency virus) [Complete proteome]

Taxonomic identifier

402771 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Primate lentivirus group › Simian immunodeficiency virus › Simian immunodeficiency virus - cpz

Virus host

Pan (chimpanzees) [TaxID: 9596]

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu By similarity. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex By similarity. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers By similarity. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 By similarity.
Subunit structure	Matrix protein p17 is a trimer. Interacts with gp120. p6-gag interacts with host TSG101 By similarity.
Subcellular location	Matrix protein p17: Virion Potential. Host nucleus By similarity. Host cytoplasm By similarity. Host cell membrane; Lipid-anchor Potential. Note: Following virus entry, the nuclear localization signal (NLS) of the matrix protein participates with Vpr to the nuclear localization of the viral genome. During virus production, the nuclear export activity of the matrix protein counteracts the NLS to maintain the Gag and Gag-Pol polyproteins in the cytoplasm, thereby directing unspliced RNA to the plasma membrane By similarity. Capsid protein p24: Virion Potential. Nucleocapsid protein p7: Virion Potential.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. p6-gag contains two L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a LYPX(n)L motif which interacts with PDCD6IP/AIP1 By similarity.
Post-translational modification	Capsid protein p24 is phosphorylated By similarity. Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.
Sequence similarities	Belongs to the primate lentivirus group gag polyprotein family. Contains 2 CCHC-type zinc fingers.

Ontologies

Keywords
Biological process	Host-virus interaction Viral budding Viral budding via the host ESCRT complexes Virus exit from host cell
Cellular component	Host cell membrane Host cytoplasm Host membrane Host nucleus Membrane Virion
Coding sequence diversity	Ribosomal frameshifting
Domain	Repeat Zinc-finger
Ligand	Metal-binding RNA-binding Viral nucleoprotein Zinc
Molecular function	Capsid protein
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	viral release from host cell Inferred from electronic annotation. Source: UniProtKB-KW virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Note: Translation results in the formation of the Gag polyprotein most of the time. Ribosomal frameshifting at the gag-pol genes boundary occurs at low frequency and produces the Gag-Pol polyprotein. This strategy of translation probably allows the virus to modulate the quantity of each viral protein. Maintenance of a correct Gag to Gag-Pol ratio is essential for RNA dimerization and viral infectivity.

Isoform Gag polyprotein (identifier: P17282-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by conventional translation.

Isoform Gag-Pol polyprotein (identifier: P17283-1)

The sequence of this isoform can be found in the external entry P17283.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	2 – 508	507	Gag polyprotein By similarity		PRO_0000316150
	Chain	2 – 140	139	Matrix protein p17 By similarity		PRO_0000038644
	Chain	141 – 371	231	Capsid protein p24 By similarity		PRO_0000038645
	Peptide	372 – 387	16	Spacer peptide p2 By similarity		PRO_0000316151
	Chain	388 – 442	55	Nucleocapsid protein p7 By similarity		PRO_0000038646
	Peptide	443 – 458	16	Spacer peptide p1 By similarity		PRO_0000316152
	Chain	459 – 508	50	p6-gag By similarity		PRO_0000316153
Regions
	Zinc finger	400 – 417	18	CCHC-type 1
	Zinc finger	421 – 438	18	CCHC-type 2
	Motif	16 – 22	7	Nuclear export signal By similarity
	Motif	26 – 32	7	Nuclear localization signal By similarity
	Motif	465 – 468	4	PTAP/PSAP motif
	Motif	491 – 500	10	LYPX(n)L motif
Sites
	Site	140 – 141	2	Cleavage; by viral protease By similarity
	Site	371 – 372	2	Cleavage; by viral protease By similarity
	Site	387 – 388	2	Cleavage; by viral protease By similarity
	Site	442 – 443	2	Cleavage; by viral protease By similarity
	Site	458 – 459	2	Cleavage; by viral protease By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine; by host By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform Gag polyprotein [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 6FC992B9EB4CBB5D	FASTA	508	55,963
	10 20 30 40 50 60 MGARASVLTG GKLDRWEKVR LRPGGRKRYM MKHLVWASRE LERFACDPGL MESKEGCTKL 70 80 90 100 110 120 LQQLEPALKT GSEGLRSLFN TLAVLWCIHS DITVEDTQKA LEQLKRHHGE QQSKTESNSG 130 140 150 160 170 180 SREGGASQGA SASAGISGNY PLVQNAQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF 190 200 210 220 230 240 SALSEGALPQ DVNTMLNAVG GHQGAMQVLK EVINEEAAEW DRLHPTHAGP IAPGQLREPR 250 260 270 280 290 300 GSDIAGTTST LQEQIGWTTA NPPIPVGDVY RRWVILGLNK VVRMYCPVSI LDIRQGPKEP 310 320 330 340 350 360 FRDYVDRFYK TLRAEQASQE VKNWMTDTLL VQNANPDCKQ ILKALGPGAT LEEMMTACQG 370 380 390 400 410 420 VGGPSHKARV LAEAMSMVQN QGRADVFFQK GQGAGPKRKI KCFNCGKEGH LARNCKAPRR 430 440 450 460 470 480 KGCWRCGQEG HQMKDCTGRQ VNFLGKGWPS RSGRPGNFVQ NRTEPTAPPI ESYGYQEEEK 490 500 SQEKKEGESS LYPPTSLKSL FGSDPSSQ
	Isoform Gag-Pol polyprotein [UniParc]. See P17283.		–	–

References

[1]	"Genetic organization of a chimpanzee lentivirus related to HIV-1." Huet T., Cheynier R., Meyerhans A., Roelants G., Wain-Hobson S. Nature 345:356-359(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52154 Genomic RNA. Translation: CAA36401.1.
PIR	FOLJSI. S09983.
3D structure databases
ProteinModelPortal	P17282.
SMR	P17282. Positions 2-442, 459-508.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1200.30. 1 hit. 1.10.150.90. 1 hit. 1.10.375.10. 1 hit. 4.10.60.10. 1 hit.
InterPro	IPR000721. Gag_p24. IPR014817. Gag_p6. IPR000071. Lentvrl_matrix_N. IPR012344. Matrix_N_HIV/RSV. IPR008916. Retrov_capsid_C. IPR008919. Retrov_capsid_N. IPR010999. Retrovr_matrix_N. IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF00540. Gag_p17. 1 hit. PF00607. Gag_p24. 1 hit. PF08705. Gag_p6. 1 hit. PF00098. zf-CCHC. 2 hits. [Graphical view]
PRINTS	PR00234. HIV1MATRIX.
SMART	SM00343. ZnF_C2HC. 2 hits. [Graphical view]
SUPFAM	SSF47353. Retrov_capsid_C. 1 hit. SSF47943. Retrov_capsid_N. 1 hit. SSF47836. Retrovir_matrix. 1 hit. SSF57756. SSF57756. 1 hit.
PROSITE	PS50158. ZF_CCHC. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAG_SIVCZ

Accession

Primary (citable) accession number: P17282

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program