P17275 (JUNB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor jun-B

Gene names

Name:

JUNB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Subunit structure	Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). Ref.8
Subcellular location	Nucleus.
Induction	By growth factors.
Post-translational modification	Ubiquitinated by ITCH, leading to its degradation. Ref.8
Sequence similarities	Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	chromatin Traceable author statement. Source: ProtInc nucleus Inferred from direct assay. Source: HPA
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro transcription coactivator activity Traceable author statement. Source: ProtInc transcription corepressor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BATF2	Q8N1L9	3	EBI-748062,EBI-742695
C19orf68	Q86XI8	2	EBI-748062,EBI-2682158

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Transcription factor jun-B		PRO_0000076438
Regions
	Domain	296 – 324	29	Leucine-zipper
	DNA binding	273 – 292	20	Basic motif
Amino acid modifications
	Modified residue	102	1	Phosphothreonine Ref.9
	Modified residue	104	1	Phosphothreonine Ref.9
	Modified residue	117	1	Phosphoserine Ref.9
	Modified residue	251	1	Phosphoserine Ref.9 Ref.10
	Modified residue	255	1	Phosphothreonine Ref.7 Ref.9 Ref.10
	Modified residue	259	1	Phosphoserine Ref.6 Ref.7 Ref.9 Ref.10
Natural variations
	Natural variant	230	1	L → V. Ref.4 Corresponds to variant rs17880705 [ dbSNP | Ensembl ].		VAR_021081
Experimental info
	Sequence conflict	124	1	A → G in AAH09465. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P17275 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: DF8CD1CD4409C6BE	FASTA	347	35,879
	10 20 30 40 50 60 MCTKMEQPFY HDDSYTATGY GRAPGGLSLH DYKLLKPSLA VNLADPYRSL KAPGARGPGP 70 80 90 100 110 120 EGGGGGSYFS GQGSDTGASL KLASSELERL IVPNSNGVIT TTPTPPGQYF YPRGGGSGGG 130 140 150 160 170 180 AGGAGGGVTE EQEGFADGFV KALDDLHKMN HVTPPNVSLG ATGGPPAGPG GVYAGPEPPP 190 200 210 220 230 240 VYTNLSSYSP ASASSGGAGA AVGTGSSYPT TTISYLPHAP PFAGGHPAQL GLGRGASTFK 250 260 270 280 290 300 EEPQTVPEAR SRDATPPVSP INMEDQERIK VERKRLRNRL AATKCRKRKL ERIARLEDKV 310 320 330 340 KTLKAENAGL SSTAGLLREQ VAQLKQKVMT HVSNGCQLLL GVKGHAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun." Schuette J., Viallet J., Nau M., Segal S., Fedorko J., Minna J. Cell 59:987-997(1989) [PubMed: 2513129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation of human cDNA clones of jun-related genes, jun-B and jun-D." Nomura N., Ide M., Sasamoto S., Matsui M., Date T., Ishizaki R. Nucleic Acids Res. 18:3047-3048(1990) [PubMed: 2112242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complex genetic organization of junB: multiple blocks of flanking evolutionarily conserved sequence at the murine and human junB loci." Phinney D.G., Tseng S.W., Ryder K. Genomics 28:228-234(1995) [PubMed: 8530030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[4]	NIEHS SNPs program Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-230.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-255 AND SER-259, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Negative regulation of the E3 ubiquitin ligase itch via Fyn-mediated tyrosine phosphorylation." Yang C., Zhou W., Jeon M.S., Demydenko D., Harada Y., Zhou H., Liu Y.C. Mol. Cell 21:135-141(2006) [PubMed: 16387660] [Abstract] Cited for: INTERACTION WITH ITCH, UBIQUITINATION.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-102; THR-104; SER-117; SER-251; THR-255 AND SER-259, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251; THR-255 AND SER-259, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29039 Genomic DNA. Translation: AAA59198.1. X51345 mRNA. Translation: CAA35738.1. U20734 Genomic DNA. Translation: AAA74915.1. AY751746 Genomic DNA. Translation: AAU43800.1. BC004250 mRNA. Translation: AAH04250.1. BC009465 mRNA. Translation: AAH09465.1. BC009466 mRNA. Translation: AAH09466.1.
IPI	IPI00013439.
PIR	TVHUJB. S10183.
RefSeq	NP_002220.1. NM_002229.2.
UniGene	Hs.25292.
3D structure databases
ProteinModelPortal	P17275.
SMR	P17275. Positions 272-329.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1052N.
IntAct	P17275. 22 interactions.
MINT	MINT-205420.
STRING	P17275.
PTM databases
PhosphoSite	P17275.
Polymorphism databases
DMDM	135304.
Proteomic databases
PRIDE	P17275.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000302754; ENSP00000303315; ENSG00000171223.
GeneID	3726.
KEGG	hsa:3726.
UCSC	uc002mvc.1. human.
Organism-specific databases
CTD	3726.
GeneCards	GC19P012902.
H-InvDB	HIX0014804.
HGNC	HGNC:6205. JUNB.
HPA	CAB004464. HPA019149.
MIM	165161. gene.
neXtProt	NX_P17275.
PharmGKB	PA30007.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17996.
HOGENOM	HBG446073.
HOVERGEN	HBG001722.
InParanoid	P17275.
OMA	NGCQLLL.
OrthoDB	EOG4RV2RS.
PhylomeDB	P17275.
Enzyme and pathway databases
Pathway_Interaction_DB	nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes. tcrcalciumpathway. Calcium signaling in the CD4+ TCR pathway. cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells. il6_7pathway. IL6-mediated signaling events.
Gene expression databases
ArrayExpress	P17275.
Bgee	P17275.
CleanEx	HS_JUNB.
Genevestigator	P17275.
GermOnline	ENSG00000171223. Homo sapiens.
Family and domain databases
InterPro	IPR004827. bZIP. IPR011616. bZIP_1. IPR008917. Euk_TF_DNA-bd. IPR005643. JNK. IPR002112. Leuzip_Jun. [Graphical view]
Gene3D	G3DSA:1.10.880.10. G3DSA:1.10.880.10. 1 hit.
KO	K09028.
Pfam	PF00170. bZIP_1. 1 hit. PF03957. Jun. 1 hit. [Graphical view]
PRINTS	PR00043. LEUZIPPRJUN.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
SUPFAM	SSF47454. Euk_transcr_DNA. 1 hit.
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	14587.
SOURCE	Search...

Entry information

Entry name

JUNB_HUMAN

Accession

Primary (citable) accession number: P17275
Secondary accession number(s): Q96GH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	January 25, 2012
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.