P17275 (JUNB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor jun-B

Gene names

Name:

JUNB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Subunit structure	Binds DNA as a homodimer or as a heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). Ref.6
Subcellular location	Nucleus.
Induction	By growth factors.
Post-translational modification	Ubiquitinated by ITCH, leading to its degradation. Ref.6
Sequence similarities	Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to calcium ion Inferred from electronic annotation. Source: Compara cellular response to hormone stimulus Inferred from electronic annotation. Source: Compara decidualization Inferred from electronic annotation. Source: Compara embryonic process involved in female pregnancy Inferred from electronic annotation. Source: Compara labyrinthine layer blood vessel development Inferred from electronic annotation. Source: Compara osteoblast differentiation Inferred from electronic annotation. Source: Compara osteoblast proliferation Inferred from electronic annotation. Source: Compara osteoclast differentiation Inferred from electronic annotation. Source: Compara positive regulation of cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: Reactome regulation of cell cycle Inferred from electronic annotation. Source: Compara response to cAMP Inferred from electronic annotation. Source: Compara response to corticosterone stimulus Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to light stimulus Inferred from electronic annotation. Source: Compara response to mechanical stimulus Inferred from electronic annotation. Source: Compara response to peptide hormone stimulus Inferred from electronic annotation. Source: Compara response to progesterone stimulus Inferred from electronic annotation. Source: Compara transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome transforming growth factor beta receptor signaling pathway Traceable author statement. Source: Reactome trophectodermal cell differentiation Inferred from electronic annotation. Source: Compara vasculogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	chromatin Traceable author statement Ref.1. Source: ProtInc nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Traceable author statement PubMed 10764760. Source: ProtInc RNA polymerase II regulatory region sequence-specific DNA binding Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara transcription coactivator activity Traceable author statement Ref.1. Source: ProtInc transcription corepressor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BATF2	Q8N1L9	3	EBI-748062,EBI-742695
C19orf68	Q86XI8	2	EBI-748062,EBI-2682158

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Transcription factor jun-B		PRO_0000076438
Regions
	Domain	268 – 331	64	bZIP
	Region	268 – 295	28	Basic motif By similarity
	Region	296 – 324	29	Leucine-zipper By similarity
Amino acid modifications
	Modified residue	102	1	Phosphothreonine Ref.7
	Modified residue	104	1	Phosphothreonine Ref.7 Ref.9
	Modified residue	117	1	Phosphoserine Ref.7 Ref.9
	Modified residue	251	1	Phosphoserine Ref.8 Ref.9
	Modified residue	255	1	Phosphothreonine Ref.8 Ref.9 Ref.10
	Modified residue	259	1	Phosphoserine Ref.8 Ref.10
Natural variations
	Natural variant	230	1	L → V. Ref.4 Corresponds to variant rs17880705 [ dbSNP | Ensembl ].		VAR_021081
Experimental info
	Sequence conflict	124	1	A → G in AAH09465. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P17275 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: DF8CD1CD4409C6BE	FASTA	347	35,879
	10 20 30 40 50 60 MCTKMEQPFY HDDSYTATGY GRAPGGLSLH DYKLLKPSLA VNLADPYRSL KAPGARGPGP 70 80 90 100 110 120 EGGGGGSYFS GQGSDTGASL KLASSELERL IVPNSNGVIT TTPTPPGQYF YPRGGGSGGG 130 140 150 160 170 180 AGGAGGGVTE EQEGFADGFV KALDDLHKMN HVTPPNVSLG ATGGPPAGPG GVYAGPEPPP 190 200 210 220 230 240 VYTNLSSYSP ASASSGGAGA AVGTGSSYPT TTISYLPHAP PFAGGHPAQL GLGRGASTFK 250 260 270 280 290 300 EEPQTVPEAR SRDATPPVSP INMEDQERIK VERKRLRNRL AATKCRKRKL ERIARLEDKV 310 320 330 340 KTLKAENAGL SSTAGLLREQ VAQLKQKVMT HVSNGCQLLL GVKGHAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun." Schuette J., Viallet J., Nau M., Segal S., Fedorko J., Minna J. Cell 59:987-997(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation of human cDNA clones of jun-related genes, jun-B and jun-D." Nomura N., Ide M., Sasamoto S., Matsui M., Date T., Ishizaki R. Nucleic Acids Res. 18:3047-3048(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complex genetic organization of junB: multiple blocks of flanking evolutionarily conserved sequence at the murine and human junB loci." Phinney D.G., Tseng S.W., Ryder K. Genomics 28:228-234(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[4]	NIEHS SNPs program Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-230.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[6]	"Negative regulation of the E3 ubiquitin ligase itch via Fyn-mediated tyrosine phosphorylation." Yang C., Zhou W., Jeon M.S., Demydenko D., Harada Y., Zhou H., Liu Y.C. Mol. Cell 21:135-141(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ITCH, UBIQUITINATION.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-102; THR-104 AND SER-117, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-251; THR-255 AND SER-259, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-104; SER-117; SER-251 AND THR-255, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-255 AND SER-259, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29039 Genomic DNA. Translation: AAA59198.1. X51345 mRNA. Translation: CAA35738.1. U20734 Genomic DNA. Translation: AAA74915.1. AY751746 Genomic DNA. Translation: AAU43800.1. BC004250 mRNA. Translation: AAH04250.1. BC009465 mRNA. Translation: AAH09465.1. BC009466 mRNA. Translation: AAH09466.1.
IPI	IPI00013439.
PIR	TVHUJB. S10183.
RefSeq	NP_002220.1. NM_002229.2.
UniGene	Hs.25292.
3D structure databases
ProteinModelPortal	P17275.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1052N.
IntAct	P17275. 22 interactions.
MINT	MINT-205420.
STRING	9606.ENSP00000303315.
PTM databases
PhosphoSite	P17275.
Polymorphism databases
DMDM	135304.
Proteomic databases
PaxDb	P17275.
PRIDE	P17275.
Protocols and materials databases
DNASU	3726.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000302754; ENSP00000303315; ENSG00000171223.
GeneID	3726.
KEGG	hsa:3726.
UCSC	uc002mvc.3. human.
Organism-specific databases
CTD	3726.
GeneCards	GC19P012902.
HGNC	HGNC:6205. JUNB.
HPA	CAB004464. HPA019149.
MIM	165161. gene.
neXtProt	NX_P17275.
PharmGKB	PA30007.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG283376.
HOGENOM	HOG000006648.
HOVERGEN	HBG001722.
InParanoid	P17275.
KO	K09028.
OMA	LADPYRS.
OrthoDB	EOG4RV2RS.
PhylomeDB	P17275.
Enzyme and pathway databases
Pathway_Interaction_DB	nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes. tcrcalciumpathway. Calcium signaling in the CD4+ TCR pathway. cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells. il6_7pathway. IL6-mediated signaling events.
Reactome	REACT_111102. Signal Transduction. REACT_71. Gene Expression.
SignaLink	P17275.
Gene expression databases
ArrayExpress	P17275.
Bgee	P17275.
CleanEx	HS_JUNB.
Genevestigator	P17275.
GermOnline	ENSG00000171223. Homo sapiens.
Family and domain databases
Gene3D	1.10.880.10. 1 hit.
InterPro	IPR004827. bZIP. IPR008917. Euk_TF_DNA-bd. IPR005643. JNK. IPR002112. Leuzip_Jun. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. PF03957. Jun. 1 hit. [Graphical view]
PRINTS	PR00043. LEUZIPPRJUN.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
SUPFAM	SSF47454. Euk_transcr_DNA. 1 hit.
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3726.
NextBio	14587.
SOURCE	Search...

Entry information

Entry name

JUNB_HUMAN

Accession

Primary (citable) accession number: P17275
Secondary accession number(s): Q96GH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 29, 2013
This is version 145 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.