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Protein

Gastric intrinsic factor

Gene

Gif

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171CobalaminBy similarity1
Binding sitei222CobalaminBy similarity1
Binding sitei270CobalaminBy similarity1

GO - Molecular functioni

  • cobalamin binding Source: RGD
  • vitamin binding Source: RGD

GO - Biological processi

  • cobalamin metabolic process Source: RGD
  • cobalamin transport Source: InterPro
  • cobalt ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cobalt transport, Ion transport, Transport

Keywords - Ligandi

Cobalt

Names & Taxonomyi

Protein namesi
Recommended name:
Gastric intrinsic factor
Alternative name(s):
Intrinsic factor
Short name:
IF
Short name:
INF
Gene namesi
Name:Gif
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62084. Gif.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
ChainiPRO_000000556019 – 417Gastric intrinsic factorAdd BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 246By similarity
Disulfide bondi103 ↔ 288By similarity
Disulfide bondi143 ↔ 182By similarity
Modified residuei191PhosphoserineCombined sources1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Glycosylationi311N-linked (GlcNAc...)Sequence analysis1
Glycosylationi330N-linked (GlcNAc...)Sequence analysis1
Glycosylationi413N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP17267.
PRIDEiP17267.

PTM databases

iPTMnetiP17267.
PhosphoSitePlusiP17267.

Expressioni

Tissue specificityi

Gastric mucosa.

Interactioni

Subunit structurei

Interacts with CUBN (via CUB domains).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028507.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 370Cobalamin bindingBy similarity6
Regioni386 – 395Cobalamin bindingBy similarity10

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVBN. Eukaryota.
ENOG410XSU1. LUCA.
HOGENOMiHOG000013214.
HOVERGENiHBG006133.
InParanoidiP17267.
KOiK14615.
PhylomeDBiP17267.
TreeFamiTF333092.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWLSFYLLN VLWAVAGTST RAQRSCSVPP DQQPWVNGLQ LLMENSVTES
60 70 80 90 100
DLPNPSILIA MNLASTYNLE AQKLLTYQLM ASDSADLTNG QLALTIMALT
110 120 130 140 150
SSCRDPGSKV SILQKNMESW TPSNLGAESS SFYGPALAIL ALCQKNSEAT
160 170 180 190 200
LPIAVRFAKT LMMESSPFSV DTGAVATLAL TCMYNRIPVG SQENYRDLFG
210 220 230 240 250
QALKVIVDNI SLRIKADGII GDIYSTGLAM QALSVTPEQP TKEWDCEKTM
260 270 280 290 300
YTILKEIKQG KFQNPMSIAQ ILPSLKGKTY LDVPQVTCGP DHEVPPTLTD
310 320 330 340 350
YPTPVPTSIS NITVIYTINN QLRGVDLLFN VTIEVSVKSG SVLLAVLEEA
360 370 380 390 400
QRRNHMFKFE TTMTSWGLIV SSINNIAENV KHKTYWEFLS GKTPLGEGVA
410
YYIPFNYEHI TANFTQY
Length:417
Mass (Da):45,956
Last modified:October 1, 1996 - v2
Checksum:i881BF5B2C77563C3
GO

Sequence cautioni

The sequence AAA41361 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA08140 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03577 mRNA. Translation: AAA41361.1. Different initiation.
D45200 Genomic DNA. Translation: BAA08140.1. Different initiation.
PIRiA34003.
RefSeqiNP_058858.1. NM_017162.3.
UniGeneiRn.34903.

Genome annotation databases

GeneIDi29319.
KEGGirno:29319.
UCSCiRGD:62084. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03577 mRNA. Translation: AAA41361.1. Different initiation.
D45200 Genomic DNA. Translation: BAA08140.1. Different initiation.
PIRiA34003.
RefSeqiNP_058858.1. NM_017162.3.
UniGeneiRn.34903.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028507.

PTM databases

iPTMnetiP17267.
PhosphoSitePlusiP17267.

Proteomic databases

PaxDbiP17267.
PRIDEiP17267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29319.
KEGGirno:29319.
UCSCiRGD:62084. rat.

Organism-specific databases

CTDi2694.
RGDi62084. Gif.

Phylogenomic databases

eggNOGiENOG410IVBN. Eukaryota.
ENOG410XSU1. LUCA.
HOGENOMiHOG000013214.
HOVERGENiHBG006133.
InParanoidiP17267.
KOiK14615.
PhylomeDBiP17267.
TreeFamiTF333092.

Miscellaneous databases

PROiP17267.

Family and domain databases

InterProiIPR002157. Cbl-bd_transpt_euk.
[Graphical view]
PANTHERiPTHR10559. PTHR10559. 1 hit.
PfamiPF01122. Cobalamin_bind. 1 hit.
[Graphical view]
PROSITEiPS00468. COBALAMIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF_RAT
AccessioniPrimary (citable) accession number: P17267
Secondary accession number(s): O35801
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.