Reviewed,
UniProtKB/Swiss-Prot P17260 (KRE1_YEAST)
Last modified
November 24, 2009.
Version 79.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protein KRE1 Alternative name(s): Killer toxin-resistance protein 1 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 313 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in a late stage of cell wall 1,6-beta-glucan synthesis and assembly. Has a structural, rather than enzymic, function within cell wall 1,6-beta-glucan assembly and architecture, possibly by being involved in covalently cross-linking 1,6-beta-glucans to other cell wall components such as 1,3-beta-glucan, chitin and certain mannoproteins. Acts as the plasma membrane receptor for the yeast K1 viral toxin. Ref.6 Ref.8 |
| Subcellular location | Cell membrane; Lipid-anchor › GPI-anchor. Secreted › cell wall. Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as component of the cell wall. Concentrated at the surface of mother cells. Ref.6 Ref.8 Ref.5 |
| Post-translational modification | Extensively modified; probably through addition of O-linked mannose residues. The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity. |
| Miscellaneous | Present with 623 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | To C.albicans KRE1. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell wall biogenesis/degradation |
| Cellular component | Cell membrane Cell wall Membrane Secreted |
| Domain | Repeat Signal |
| PTM | GPI-anchor Glycoprotein Lipoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell wall organization Traceable author statement. Source: SGD |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular regionInferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wallInferred from direct assay. Source: SGD plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct structural constituent of cell wall Ref.6Traceable author statement. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| OST1 | P41543 | 1 | EBI-2068892,EBI-12651 | |
| RNR1 | P21524 | 1 | EBI-2068892,EBI-15234 | |
| RNR2 | P09938 | 1 | EBI-2068892,EBI-15240 | |
| SWP1 | Q02795 | 1 | EBI-2068892,EBI-12666 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | Potential | ||||||
| Chain | 27 – 288 | 262 | Protein KRE1 | PRO_0000021561 | |||||
| Propeptide | 289 – 313 | 25 | Removed in mature form Potential | PRO_0000021562 | |||||
Regions | |||||||||
| Repeat | 72 – 86 | 15 | 1 | ||||||
| Repeat | 127 – 141 | 15 | 2 | ||||||
| Region | 72 – 141 | 70 | 2 X approximate repeats | ||||||
| Compositional bias | 52 – 58 | 7 | Poly-Ala | ||||||
| Compositional bias | 95 – 100 | 6 | Poly-Thr | ||||||
| Compositional bias | 106 – 109 | 4 | Poly-Thr | ||||||
| Compositional bias | 158 – 169 | 12 | Poly-Ser | ||||||
Amino acid modifications | |||||||||
| Lipidation | 288 | 1 | GPI-anchor amidated asparagine Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly." Boone C., Sommer S.S., Hensel A., Bussey H. J. Cell Biol. 110:1833-1843(1990) [PubMed: 2186051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88. |
| [2] | "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676. |
| [3] | "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676. |
| [4] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676. |
| [5] | "Yeast Kre1p is a cell surface O-glycoprotein." Roemer T., Bussey H. Mol. Gen. Genet. 249:209-216(1995) [PubMed: 7500943] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| [6] | "Kre1p, the plasma membrane receptor for the yeast K1 viral toxin." Breinig F., Tipper D.J., Schmitt M.J. Cell 108:395-405(2002) [PubMed: 11853673] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "Yeast Kre1p is GPI-anchored and involved in both cell wall assembly and architecture." Breinig F., Schleinkofer K., Schmitt M.J. Microbiology 150:3209-3218(2004) [PubMed: 15470101] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X51729 Genomic DNA. Translation: CAA36017.1. Z46259 Genomic DNA. Translation: CAA86375.1. Z71598 Genomic DNA. Translation: CAA96253.1. | |
| PIR | A34677. |
| RefSeq | NP_014077.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P17260. 6 interactions. |
| STRING | P17260. |
Genome annotation databases | |
| Ensembl | YNL322C; YNL322C; YNL322C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 855394. |
| KEGG | sce:YNL322C. |
| NMPDR | fig|4932.3.peg.5139. |
Organism-specific databases | |
| CYGD | YNL322c. |
| SGD | S000005266. KRE1. |
Phylogenomic databases | |
| OMA | PTSIWVT |
| OrthoDB | EOG983FMZ |
Gene expression databases | |
| ArrayExpress | P17260. |
| Genevestigator | P17260. |
| GermOnline | YNL322C. Saccharomyces cerevisiae. |
Family and domain databases | |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 979208. |
Entry information
| Entry name | KRE1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P17260 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |

Clusters with


