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P17260

- KRE1_YEAST

UniProt

P17260 - KRE1_YEAST

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Protein

Protein KRE1

Gene

KRE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in a late stage of cell wall 1,6-beta-glucan synthesis and assembly. Has a structural, rather than enzymic, function within cell wall 1,6-beta-glucan assembly and architecture, possibly by being involved in covalently cross-linking 1,6-beta-glucans to other cell wall components such as 1,3-beta-glucan, chitin and certain mannoproteins. Acts as the plasma membrane receptor for the yeast K1 viral toxin.2 Publications

GO - Molecular functioni

  1. structural constituent of cell wall Source: SGD

GO - Biological processi

  1. fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-33307-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein KRE1
Alternative name(s):
Killer toxin-resistance protein 1
Gene namesi
Name:KRE1
Ordered Locus Names:YNL322C
ORF Names:N0336
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL322c.
SGDiS000005266. KRE1.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall
Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as component of the cell wall. Concentrated at the surface of mother cells.

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 288262Protein KRE1PRO_0000021561Add
BLAST
Propeptidei289 – 31325Removed in mature formSequence AnalysisPRO_0000021562Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi288 – 2881GPI-anchor amidated asparagineSequence Analysis

Post-translational modificationi

Extensively modified; probably through addition of O-linked mannose residues.
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity).By similarity

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP17260.

Expressioni

Gene expression databases

GenevestigatoriP17260.

Interactioni

Protein-protein interaction databases

BioGridi35518. 169 interactions.
IntActiP17260. 1 interaction.
MINTiMINT-4500985.
STRINGi4932.YNL322C.

Structurei

3D structure databases

ProteinModelPortaliP17260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati72 – 86151Add
BLAST
Repeati127 – 141152Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 141702 X approximate repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi52 – 587Poly-Ala
Compositional biasi95 – 1006Poly-Thr
Compositional biasi106 – 1094Poly-Thr
Compositional biasi158 – 16912Poly-SerAdd
BLAST

Sequence similaritiesi

Belongs to the KRE1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG41048.
InParanoidiP17260.
OMAiWIQFISK.
OrthoDBiEOG7J44KS.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17260-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMRRTLLHSF ATLLLSLSLW SAAVMAAVTT QVTVVTNVAG ALVTETTIWD
60 70 80 90 100
PATAAAAATT TAQTGFFTTV FTTTNDVGTT VTLTQTVNRA TMLPTTTTST
110 120 130 140 150
SSTGKTTTTV PTATSSLSSG LYLSTVTTTN DLGTTVTLTQ TFTHSSTSAT
160 170 180 190 200
SSASSSVSSS VSSSGSSSSV KTTTSTGSAV AETGTRPDPS TDFTEPPVSA
210 220 230 240 250
VTSLSIDSYI TITEGTTSTY TTTRAPTSMW VTVVRQGNTI TVQTTFVQRF
260 270 280 290 300
SSQYVTVASP SVGSIGMGTL TGTVGVIKSA IKKTVSHNEA QHLGMSSFTS
310
ILGGLLTVLI WFL
Length:313
Mass (Da):32,158
Last modified:August 1, 1990 - v1
Checksum:iB01A5DA0D3781E88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51729 Genomic DNA. Translation: CAA36017.1.
Z46259 Genomic DNA. Translation: CAA86375.1.
Z71598 Genomic DNA. Translation: CAA96253.1.
BK006947 Genomic DNA. Translation: DAA10240.1.
PIRiA34677.
RefSeqiNP_014077.1. NM_001183160.1.

Genome annotation databases

EnsemblFungiiYNL322C; YNL322C; YNL322C.
GeneIDi855394.
KEGGisce:YNL322C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51729 Genomic DNA. Translation: CAA36017.1 .
Z46259 Genomic DNA. Translation: CAA86375.1 .
Z71598 Genomic DNA. Translation: CAA96253.1 .
BK006947 Genomic DNA. Translation: DAA10240.1 .
PIRi A34677.
RefSeqi NP_014077.1. NM_001183160.1.

3D structure databases

ProteinModelPortali P17260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35518. 169 interactions.
IntActi P17260. 1 interaction.
MINTi MINT-4500985.
STRINGi 4932.YNL322C.

Proteomic databases

PaxDbi P17260.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL322C ; YNL322C ; YNL322C .
GeneIDi 855394.
KEGGi sce:YNL322C.

Organism-specific databases

CYGDi YNL322c.
SGDi S000005266. KRE1.

Phylogenomic databases

eggNOGi NOG41048.
InParanoidi P17260.
OMAi WIQFISK.
OrthoDBi EOG7J44KS.

Enzyme and pathway databases

BioCyci YEAST:G3O-33307-MONOMER.

Miscellaneous databases

NextBioi 979208.

Gene expression databases

Genevestigatori P17260.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly."
    Boone C., Sommer S.S., Hensel A., Bussey H.
    J. Cell Biol. 110:1833-1843(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
    Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
    Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  3. "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
    Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
    Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Yeast Kre1p is a cell surface O-glycoprotein."
    Roemer T., Bussey H.
    Mol. Gen. Genet. 249:209-216(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Kre1p, the plasma membrane receptor for the yeast K1 viral toxin."
    Breinig F., Tipper D.J., Schmitt M.J.
    Cell 108:395-405(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Yeast Kre1p is GPI-anchored and involved in both cell wall assembly and architecture."
    Breinig F., Schleinkofer K., Schmitt M.J.
    Microbiology 150:3209-3218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKRE1_YEAST
AccessioniPrimary (citable) accession number: P17260
Secondary accession number(s): D6W0M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3