Protein KRE1 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P17260 (KRE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein KRE1

Alternative name(s):
Killer toxin-resistance protein 1

Gene names

Name:	KRE1
Ordered Locus Names:	YNL322C
ORF Names:	N0336

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a late stage of cell wall 1,6-beta-glucan synthesis and assembly. Has a structural, rather than enzymic, function within cell wall 1,6-beta-glucan assembly and architecture, possibly by being involved in covalently cross-linking 1,6-beta-glucans to other cell wall components such as 1,3-beta-glucan, chitin and certain mannoproteins. Acts as the plasma membrane receptor for the yeast K1 viral toxin. Ref.7 Ref.9
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Secreted › cell wall. Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as component of the cell wall. Concentrated at the surface of mother cells. Ref.6 Ref.7 Ref.9
Post-translational modification	Extensively modified; probably through addition of O-linked mannose residues. The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.
Miscellaneous	Present with 623 molecules/cell in log phase SD medium.
Sequence similarities	To C.albicans KRE1.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cell wall Membrane Secreted
Domain	Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fungal-type cell wall organization Traceable author statement PubMed 9791887. Source: SGD
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay PubMed 11956747. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	structural constituent of cell wall Traceable author statement Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Potential

Chain

27 – 288

262

Protein KRE1

PRO_0000021561

Propeptide

289 – 313

Removed in mature form Potential

PRO_0000021562

Regions

Repeat

72 – 86

Repeat

127 – 141

Region

72 – 141

2 X approximate repeats

Compositional bias

52 – 58

Poly-Ala

Compositional bias

95 – 100

Poly-Thr

Compositional bias

106 – 109

Poly-Thr

Compositional bias

158 – 169

Poly-Ser

Amino acid modifications

Lipidation

288

GPI-anchor amidated asparagine Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P17260 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: B01A5DA0D3781E88
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FASTA

313

32,158

        10         20         30         40         50         60 
MMRRTLLHSF ATLLLSLSLW SAAVMAAVTT QVTVVTNVAG ALVTETTIWD PATAAAAATT 

        70         80         90        100        110        120 
TAQTGFFTTV FTTTNDVGTT VTLTQTVNRA TMLPTTTTST SSTGKTTTTV PTATSSLSSG 

       130        140        150        160        170        180 
LYLSTVTTTN DLGTTVTLTQ TFTHSSTSAT SSASSSVSSS VSSSGSSSSV KTTTSTGSAV 

       190        200        210        220        230        240 
AETGTRPDPS TDFTEPPVSA VTSLSIDSYI TITEGTTSTY TTTRAPTSMW VTVVRQGNTI 

       250        260        270        280        290        300 
TVQTTFVQRF SSQYVTVASP SVGSIGMGTL TGTVGVIKSA IKKTVSHNEA QHLGMSSFTS 

       310 
ILGGLLTVLI WFL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly." Boone C., Sommer S.S., Hensel A., Bussey H. J. Cell Biol. 110:1833-1843(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[3]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Yeast Kre1p is a cell surface O-glycoprotein." Roemer T., Bussey H. Mol. Gen. Genet. 249:209-216(1995) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Kre1p, the plasma membrane receptor for the yeast K1 viral toxin." Breinig F., Tipper D.J., Schmitt M.J. Cell 108:395-405(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Yeast Kre1p is GPI-anchored and involved in both cell wall assembly and architecture." Breinig F., Schleinkofer K., Schmitt M.J. Microbiology 150:3209-3218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X51729 Genomic DNA. Translation: CAA36017.1. Z46259 Genomic DNA. Translation: CAA86375.1. Z71598 Genomic DNA. Translation: CAA96253.1. BK006947 Genomic DNA. Translation: DAA10240.1.
PIR	A34677.
RefSeq	NP_014077.1. NM_001183160.1.
3D structure databases
ProteinModelPortal	P17260.
ModBase	Search...
Protein-protein interaction databases
IntAct	P17260. 1 interaction.
MINT	MINT-4500985.
STRING	4932.YNL322C.
Proteomic databases
PaxDb	P17260.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL322C; YNL322C; YNL322C.
GeneID	855394.
KEGG	sce:YNL322C.
Organism-specific databases
CYGD	YNL322c.
SGD	S000005266. KRE1.
Phylogenomic databases
eggNOG	NOG41048.
GeneTree	ENSGT00700000104854.
OMA	YLEVASP.
OrthoDB	EOG4WM84M.
Gene expression databases
Genevestigator	P17260.
GermOnline	YNL322C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	979208.

Entry information

Entry name

KRE1_YEAST

Accession

Primary (citable) accession number: P17260
Secondary accession number(s): D6W0M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents