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P17260 (KRE1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein KRE1
Alternative name(s):
Killer toxin-resistance protein 1
Gene names
Name:KRE1
Ordered Locus Names:YNL322C
ORF Names:N0336
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a late stage of cell wall 1,6-beta-glucan synthesis and assembly. Has a structural, rather than enzymic, function within cell wall 1,6-beta-glucan assembly and architecture, possibly by being involved in covalently cross-linking 1,6-beta-glucans to other cell wall components such as 1,3-beta-glucan, chitin and certain mannoproteins. Acts as the plasma membrane receptor for the yeast K1 viral toxin. Ref.7 Ref.9

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall. Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as component of the cell wall. Concentrated at the surface of mother cells. Ref.6 Ref.7 Ref.9

Post-translational modification

Extensively modified; probably through addition of O-linked mannose residues.

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Miscellaneous

Present with 623 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the KRE1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 288262Protein KRE1
PRO_0000021561
Propeptide289 – 31325Removed in mature form Potential
PRO_0000021562

Regions

Repeat72 – 86151
Repeat127 – 141152
Region72 – 141702 X approximate repeats
Compositional bias52 – 587Poly-Ala
Compositional bias95 – 1006Poly-Thr
Compositional bias106 – 1094Poly-Thr
Compositional bias158 – 16912Poly-Ser

Amino acid modifications

Lipidation2881GPI-anchor amidated asparagine Potential

Sequences

Sequence LengthMass (Da)Tools
P17260 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: B01A5DA0D3781E88

FASTA31332,158
        10         20         30         40         50         60 
MMRRTLLHSF ATLLLSLSLW SAAVMAAVTT QVTVVTNVAG ALVTETTIWD PATAAAAATT 

        70         80         90        100        110        120 
TAQTGFFTTV FTTTNDVGTT VTLTQTVNRA TMLPTTTTST SSTGKTTTTV PTATSSLSSG 

       130        140        150        160        170        180 
LYLSTVTTTN DLGTTVTLTQ TFTHSSTSAT SSASSSVSSS VSSSGSSSSV KTTTSTGSAV 

       190        200        210        220        230        240 
AETGTRPDPS TDFTEPPVSA VTSLSIDSYI TITEGTTSTY TTTRAPTSMW VTVVRQGNTI 

       250        260        270        280        290        300 
TVQTTFVQRF SSQYVTVASP SVGSIGMGTL TGTVGVIKSA IKKTVSHNEA QHLGMSSFTS 

       310 
ILGGLLTVLI WFL 

« Hide

References

« Hide 'large scale' references
[1]"Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly."
Boone C., Sommer S.S., Hensel A., Bussey H.
J. Cell Biol. 110:1833-1843(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[2]"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[3]"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / FY1676.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Yeast Kre1p is a cell surface O-glycoprotein."
Roemer T., Bussey H.
Mol. Gen. Genet. 249:209-216(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Kre1p, the plasma membrane receptor for the yeast K1 viral toxin."
Breinig F., Tipper D.J., Schmitt M.J.
Cell 108:395-405(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Yeast Kre1p is GPI-anchored and involved in both cell wall assembly and architecture."
Breinig F., Schleinkofer K., Schmitt M.J.
Microbiology 150:3209-3218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X51729 Genomic DNA. Translation: CAA36017.1.
Z46259 Genomic DNA. Translation: CAA86375.1.
Z71598 Genomic DNA. Translation: CAA96253.1.
BK006947 Genomic DNA. Translation: DAA10240.1.
PIRA34677.
RefSeqNP_014077.1. NM_001183160.1.

3D structure databases

ProteinModelPortalP17260.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35518. 169 interactions.
IntActP17260. 1 interaction.
MINTMINT-4500985.
STRING4932.YNL322C.

Proteomic databases

PaxDbP17260.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL322C; YNL322C; YNL322C.
GeneID855394.
KEGGsce:YNL322C.

Organism-specific databases

CYGDYNL322c.
SGDS000005266. KRE1.

Phylogenomic databases

eggNOGNOG41048.
OMAYVSTITT.
OrthoDBEOG7J44KS.

Enzyme and pathway databases

BioCycYEAST:G3O-33307-MONOMER.

Gene expression databases

GenevestigatorP17260.

Family and domain databases

ProtoNetSearch...

Other

NextBio979208.

Entry information

Entry nameKRE1_YEAST
AccessionPrimary (citable) accession number: P17260
Secondary accession number(s): D6W0M4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: February 19, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families