Reviewed, UniProtKB/Swiss-Prot P17260 (KRE1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein KRE1
Alternative name(s):
    Killer toxin-resistance protein 1

Gene names

Name:	KRE1
Ordered Locus Names:	YNL322C
ORF Names:	N0336

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in a late stage of cell wall 1,6-beta-glucan synthesis and assembly. Has a structural, rather than enzymic, function within cell wall 1,6-beta-glucan assembly and architecture, possibly by being involved in covalently cross-linking 1,6-beta-glucans to other cell wall components such as 1,3-beta-glucan, chitin and certain mannoproteins. Acts as the plasma membrane receptor for the yeast K1 viral toxin. Ref.6 Ref.8
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Secreted › cell wall. Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as component of the cell wall. Concentrated at the surface of mother cells. Ref.6 Ref.8 Ref.5
Post-translational modification	Extensively modified; probably through addition of O-linked mannose residues. The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.
Miscellaneous	Present with 623 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	To C.albicans KRE1.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cell wall Membrane Secreted
Domain	Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Traceable author statement. Source: SGD
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of cell wall Ref.6 Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
OST1	P41543	1	EBI-2068892,EBI-12651
RNR1	P21524	1	EBI-2068892,EBI-15234
RNR2	P09938	1	EBI-2068892,EBI-15240
SWP1	Q02795	1	EBI-2068892,EBI-12666

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 288	262	Protein KRE1		PRO_0000021561
	Propeptide	289 – 313	25	Removed in mature form Potential		PRO_0000021562
Regions
	Repeat	72 – 86	15	1
	Repeat	127 – 141	15	2
	Region	72 – 141	70	2 X approximate repeats
	Compositional bias	52 – 58	7	Poly-Ala
	Compositional bias	95 – 100	6	Poly-Thr
	Compositional bias	106 – 109	4	Poly-Thr
	Compositional bias	158 – 169	12	Poly-Ser
Amino acid modifications
	Lipidation	288	1	GPI-anchor amidated asparagine Potential

Sequences

	Sequence		Length	Mass (Da)
	P17260-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: B01A5DA0D3781E88	FASTA	313	32,158
	10 20 30 40 50 60 MMRRTLLHSF ATLLLSLSLW SAAVMAAVTT QVTVVTNVAG ALVTETTIWD PATAAAAATT 70 80 90 100 110 120 TAQTGFFTTV FTTTNDVGTT VTLTQTVNRA TMLPTTTTST SSTGKTTTTV PTATSSLSSG 130 140 150 160 170 180 LYLSTVTTTN DLGTTVTLTQ TFTHSSTSAT SSASSSVSSS VSSSGSSSSV KTTTSTGSAV 190 200 210 220 230 240 AETGTRPDPS TDFTEPPVSA VTSLSIDSYI TITEGTTSTY TTTRAPTSMW VTVVRQGNTI 250 260 270 280 290 300 TVQTTFVQRF SSQYVTVASP SVGSIGMGTL TGTVGVIKSA IKKTVSHNEA QHLGMSSFTS 310 ILGGLLTVLI WFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly." Boone C., Sommer S.S., Hensel A., Bussey H. J. Cell Biol. 110:1833-1843(1990) [PubMed: 2186051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:567-572(1995) [PubMed: 7645347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[3]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676.
[5]	"Yeast Kre1p is a cell surface O-glycoprotein." Roemer T., Bussey H. Mol. Gen. Genet. 249:209-216(1995) [PubMed: 7500943] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Kre1p, the plasma membrane receptor for the yeast K1 viral toxin." Breinig F., Tipper D.J., Schmitt M.J. Cell 108:395-405(2002) [PubMed: 11853673] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Yeast Kre1p is GPI-anchored and involved in both cell wall assembly and architecture." Breinig F., Schleinkofer K., Schmitt M.J. Microbiology 150:3209-3218(2004) [PubMed: 15470101] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51729 Genomic DNA. Translation: CAA36017.1. Z46259 Genomic DNA. Translation: CAA86375.1. Z71598 Genomic DNA. Translation: CAA96253.1.
PIR	A34677.
RefSeq	NP_014077.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P17260. 6 interactions.
STRING	P17260.
Genome annotation databases
Ensembl	YNL322C; YNL322C; YNL322C; Saccharomyces cerevisiae. [Genome view]
GeneID	855394.
KEGG	sce:YNL322C.
NMPDR	fig|4932.3.peg.5139.
Organism-specific databases
CYGD	YNL322c.
SGD	S000005266. KRE1.
Phylogenomic databases
OMA	PTSIWVT
OrthoDB	EOG983FMZ
Gene expression databases
ArrayExpress	P17260.
Genevestigator	P17260.
GermOnline	YNL322C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	979208.

Entry information

Entry name

KRE1_YEAST

Accession

Primary (citable) accession number: P17260

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	November 24, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)