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P17260

- KRE1_YEAST

UniProt

P17260 - KRE1_YEAST

Protein

Protein KRE1

Gene

KRE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Involved in a late stage of cell wall 1,6-beta-glucan synthesis and assembly. Has a structural, rather than enzymic, function within cell wall 1,6-beta-glucan assembly and architecture, possibly by being involved in covalently cross-linking 1,6-beta-glucans to other cell wall components such as 1,3-beta-glucan, chitin and certain mannoproteins. Acts as the plasma membrane receptor for the yeast K1 viral toxin.2 Publications

    GO - Molecular functioni

    1. structural constituent of cell wall Source: SGD

    GO - Biological processi

    1. fungal-type cell wall organization Source: SGD

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33307-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein KRE1
    Alternative name(s):
    Killer toxin-resistance protein 1
    Gene namesi
    Name:KRE1
    Ordered Locus Names:YNL322C
    ORF Names:N0336
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL322c.
    SGDiS000005266. KRE1.

    Subcellular locationi

    Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall
    Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as component of the cell wall. Concentrated at the surface of mother cells.

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-KW
    3. fungal-type cell wall Source: SGD
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 288262Protein KRE1PRO_0000021561Add
    BLAST
    Propeptidei289 – 31325Removed in mature formSequence AnalysisPRO_0000021562Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi288 – 2881GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    Extensively modified; probably through addition of O-linked mannose residues.
    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP17260.

    Expressioni

    Gene expression databases

    GenevestigatoriP17260.

    Interactioni

    Protein-protein interaction databases

    BioGridi35518. 169 interactions.
    IntActiP17260. 1 interaction.
    MINTiMINT-4500985.
    STRINGi4932.YNL322C.

    Structurei

    3D structure databases

    ProteinModelPortaliP17260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati72 – 86151Add
    BLAST
    Repeati127 – 141152Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni72 – 141702 X approximate repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi52 – 587Poly-Ala
    Compositional biasi95 – 1006Poly-Thr
    Compositional biasi106 – 1094Poly-Thr
    Compositional biasi158 – 16912Poly-SerAdd
    BLAST

    Sequence similaritiesi

    Belongs to the KRE1 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG41048.
    OMAiWIQFISK.
    OrthoDBiEOG7J44KS.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P17260-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMRRTLLHSF ATLLLSLSLW SAAVMAAVTT QVTVVTNVAG ALVTETTIWD    50
    PATAAAAATT TAQTGFFTTV FTTTNDVGTT VTLTQTVNRA TMLPTTTTST 100
    SSTGKTTTTV PTATSSLSSG LYLSTVTTTN DLGTTVTLTQ TFTHSSTSAT 150
    SSASSSVSSS VSSSGSSSSV KTTTSTGSAV AETGTRPDPS TDFTEPPVSA 200
    VTSLSIDSYI TITEGTTSTY TTTRAPTSMW VTVVRQGNTI TVQTTFVQRF 250
    SSQYVTVASP SVGSIGMGTL TGTVGVIKSA IKKTVSHNEA QHLGMSSFTS 300
    ILGGLLTVLI WFL 313
    Length:313
    Mass (Da):32,158
    Last modified:August 1, 1990 - v1
    Checksum:iB01A5DA0D3781E88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51729 Genomic DNA. Translation: CAA36017.1.
    Z46259 Genomic DNA. Translation: CAA86375.1.
    Z71598 Genomic DNA. Translation: CAA96253.1.
    BK006947 Genomic DNA. Translation: DAA10240.1.
    PIRiA34677.
    RefSeqiNP_014077.1. NM_001183160.1.

    Genome annotation databases

    EnsemblFungiiYNL322C; YNL322C; YNL322C.
    GeneIDi855394.
    KEGGisce:YNL322C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51729 Genomic DNA. Translation: CAA36017.1 .
    Z46259 Genomic DNA. Translation: CAA86375.1 .
    Z71598 Genomic DNA. Translation: CAA96253.1 .
    BK006947 Genomic DNA. Translation: DAA10240.1 .
    PIRi A34677.
    RefSeqi NP_014077.1. NM_001183160.1.

    3D structure databases

    ProteinModelPortali P17260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35518. 169 interactions.
    IntActi P17260. 1 interaction.
    MINTi MINT-4500985.
    STRINGi 4932.YNL322C.

    Proteomic databases

    PaxDbi P17260.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL322C ; YNL322C ; YNL322C .
    GeneIDi 855394.
    KEGGi sce:YNL322C.

    Organism-specific databases

    CYGDi YNL322c.
    SGDi S000005266. KRE1.

    Phylogenomic databases

    eggNOGi NOG41048.
    OMAi WIQFISK.
    OrthoDBi EOG7J44KS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33307-MONOMER.

    Miscellaneous databases

    NextBioi 979208.

    Gene expression databases

    Genevestigatori P17260.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly."
      Boone C., Sommer S.S., Hensel A., Bussey H.
      J. Cell Biol. 110:1833-1843(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    2. "Sequencing analysis of a 15.4 kb fragment of yeast chromosome XIV identifies the RPD3, PAS8 and KRE1 loci, five new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:567-572(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    3. "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Yeast Kre1p is a cell surface O-glycoprotein."
      Roemer T., Bussey H.
      Mol. Gen. Genet. 249:209-216(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Kre1p, the plasma membrane receptor for the yeast K1 viral toxin."
      Breinig F., Tipper D.J., Schmitt M.J.
      Cell 108:395-405(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Yeast Kre1p is GPI-anchored and involved in both cell wall assembly and architecture."
      Breinig F., Schleinkofer K., Schmitt M.J.
      Microbiology 150:3209-3218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiKRE1_YEAST
    AccessioniPrimary (citable) accession number: P17260
    Secondary accession number(s): D6W0M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 623 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3