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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

WARS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).By similarity

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Cleaved into the following 2 chains:
Gene namesi
Name:WARS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 21

Organism-specific databases

VGNCiVGNC:36863 WARS

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367371 – 476Tryptophan--tRNA ligase, cytoplasmicAdd BLAST476
ChainiPRO_000038645976 – 476T1-TrpRSBy similarityAdd BLAST401
ChainiPRO_000038646099 – 476T2-TrpRSBy similarityAdd BLAST378

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei159N6-succinyllysineBy similarity1
Modified residuei356PhosphoserineBy similarity1

Post-translational modificationi

Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17248
PeptideAtlasiP17248
PRIDEiP17248

Expressioni

Gene expression databases

BgeeiENSBTAG00000004679

Interactioni

Subunit structurei

Homodimer. Interacts with oxidized form of GAPDH (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006139

Structurei

3D structure databases

ProteinModelPortaliP17248
SMRiP17248
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 69WHEP-TRSPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 117Dispensable to the catalytic activityAdd BLAST117

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi169 – 178"HIGH" region10
Motifi354 – 358"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2145 Eukaryota
COG0180 LUCA
GeneTreeiENSGT00390000000666
HOGENOMiHOG000224742
HOVERGENiHBG002325
InParanoidiP17248
KOiK01867
OMAiADILHPQ
OrthoDBiEOG091G09U9
TreeFamiTF105669

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR009068 S15_NS1_RNA-bd
IPR002306 Trp-tRNA-ligase
IPR000738 WHEP-TRS_dom
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PF00458 WHEP-TRS, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
SMARTiView protein in SMART
SM00991 WHEP-TRS, 1 hit
SUPFAMiSSF47060 SSF47060, 1 hit
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS00762 WHEP_TRS_1, 1 hit
PS51185 WHEP_TRS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADMSNGEQG CGSPLELFHS IAAQGELVRD LKARNAAKDE IDSAVKMLLS
60 70 80 90 100
LKTSYKAATG EDYKVDCPPG DPAPESGEGL DATEADEDFV DPWTVQTSSA
110 120 130 140 150
KGIDYDKLIV RFGSSKIDKE LVNRIERATG QRPHRFLRRG IFFSHRDMHQ
160 170 180 190 200
ILDAYENKKP FYLYTGRGPS SEAMHVGHLI PFIFTKWLQD VFNVPLVIQM
210 220 230 240 250
TDDEKYLWKD LTLDQAYGYA VENAKDIIAC GFDINKTFIF SDLDYMGMSP
260 270 280 290 300
GFYKNVVKIQ KHVTFNQVKG IFGFTDSDCI GKISFPAIQA APSFSNSFPQ
310 320 330 340 350
IFRDRTDVQC LIPCAIDQDP YFRMTRDVAP RIGYPKPALL HSTFFPALQG
360 370 380 390 400
AQTKMSASDP NSSIFLTDTA KQIKTKVNKH AFSGGRDTVE EHRQFGGNCD
410 420 430 440 450
VDVSFMYLTF FLEDDDKLEQ IRRDYTSGAM LTGELKKELI EVLQPLIAEH
460 470
QARRKEVTDE IVKEFMTPRK LSYDFQ
Length:476
Mass (Da):53,812
Last modified:July 11, 2006 - v3
Checksum:i53ABC6859A7C6632
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17L → M in CAA36356 (Ref. 3) Curated1
Sequence conflicti228 – 229IA → T (PubMed:1907847).Curated2
Sequence conflicti228 – 229IA → T (Ref. 3) Curated2
Sequence conflicti443Missing AA sequence (PubMed:2631684).Curated1
Sequence conflicti451 – 459QARRKEVTD → ESRKEVDM AA sequence (PubMed:2631684).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53918 mRNA Translation: CAA37872.1
BC102806 mRNA Translation: AAI02807.1
X52113 mRNA Translation: CAA36356.1
PIRiA40279 YWBO
RefSeqiNP_776643.1, NM_174218.1
UniGeneiBt.43956

Genome annotation databases

EnsembliENSBTAT00000006139; ENSBTAP00000006139; ENSBTAG00000004679
GeneIDi281576
KEGGibta:281576

Similar proteinsi

Entry informationi

Entry nameiSYWC_BOVIN
AccessioniPrimary (citable) accession number: P17248
Secondary accession number(s): Q3SZK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: July 11, 2006
Last modified: April 25, 2018
This is version 143 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health