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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

WARS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).By similarity

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Cleaved into the following 2 chains:
Gene namesi
Name:WARS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 21

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Tryptophan--tRNA ligase, cytoplasmicPRO_0000136737Add
BLAST
Chaini76 – 476401T1-TrpRSBy similarityPRO_0000386459Add
BLAST
Chaini99 – 476378T2-TrpRSBy similarityPRO_0000386460Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591N6-succinyllysineBy similarity

Post-translational modificationi

Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.By similarity

Proteomic databases

PaxDbiP17248.
PRIDEiP17248.

Interactioni

Subunit structurei

Homodimer. Interacts with oxidized form of GAPDH (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006139.

Structurei

3D structure databases

ProteinModelPortaliP17248.
SMRiP17248. Positions 87-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 6957WHEP-TRSPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 117117Dispensable to the catalytic activityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi169 – 17810"HIGH" region
Motifi354 – 3585"KMSKS" region

Sequence similaritiesi

Contains 1 WHEP-TRS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2145. Eukaryota.
COG0180. LUCA.
GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
HOVERGENiHBG002325.
InParanoidiP17248.
KOiK01867.
OMAiFFFSHRD.
OrthoDBiEOG7T4MK3.
TreeFamiTF105669.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR002306. Trp-tRNA-ligase.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADMSNGEQG CGSPLELFHS IAAQGELVRD LKARNAAKDE IDSAVKMLLS
60 70 80 90 100
LKTSYKAATG EDYKVDCPPG DPAPESGEGL DATEADEDFV DPWTVQTSSA
110 120 130 140 150
KGIDYDKLIV RFGSSKIDKE LVNRIERATG QRPHRFLRRG IFFSHRDMHQ
160 170 180 190 200
ILDAYENKKP FYLYTGRGPS SEAMHVGHLI PFIFTKWLQD VFNVPLVIQM
210 220 230 240 250
TDDEKYLWKD LTLDQAYGYA VENAKDIIAC GFDINKTFIF SDLDYMGMSP
260 270 280 290 300
GFYKNVVKIQ KHVTFNQVKG IFGFTDSDCI GKISFPAIQA APSFSNSFPQ
310 320 330 340 350
IFRDRTDVQC LIPCAIDQDP YFRMTRDVAP RIGYPKPALL HSTFFPALQG
360 370 380 390 400
AQTKMSASDP NSSIFLTDTA KQIKTKVNKH AFSGGRDTVE EHRQFGGNCD
410 420 430 440 450
VDVSFMYLTF FLEDDDKLEQ IRRDYTSGAM LTGELKKELI EVLQPLIAEH
460 470
QARRKEVTDE IVKEFMTPRK LSYDFQ
Length:476
Mass (Da):53,812
Last modified:July 11, 2006 - v3
Checksum:i53ABC6859A7C6632
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171L → M in CAA36356 (Ref. 3) Curated
Sequence conflicti228 – 2292IA → T (PubMed:1907847).Curated
Sequence conflicti228 – 2292IA → T (Ref. 3) Curated
Sequence conflicti443 – 4431Missing AA sequence (PubMed:2631684).Curated
Sequence conflicti451 – 4599QARRKEVTD → ESRKEVDM AA sequence (PubMed:2631684).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53918 mRNA. Translation: CAA37872.1.
BC102806 mRNA. Translation: AAI02807.1.
X52113 mRNA. Translation: CAA36356.1.
PIRiA40279. YWBO.
RefSeqiNP_776643.1. NM_174218.1.
UniGeneiBt.43956.

Genome annotation databases

EnsembliENSBTAT00000006139; ENSBTAP00000006139; ENSBTAG00000004679.
GeneIDi281576.
KEGGibta:281576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53918 mRNA. Translation: CAA37872.1.
BC102806 mRNA. Translation: AAI02807.1.
X52113 mRNA. Translation: CAA36356.1.
PIRiA40279. YWBO.
RefSeqiNP_776643.1. NM_174218.1.
UniGeneiBt.43956.

3D structure databases

ProteinModelPortaliP17248.
SMRiP17248. Positions 87-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006139.

Proteomic databases

PaxDbiP17248.
PRIDEiP17248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006139; ENSBTAP00000006139; ENSBTAG00000004679.
GeneIDi281576.
KEGGibta:281576.

Organism-specific databases

CTDi7453.

Phylogenomic databases

eggNOGiKOG2145. Eukaryota.
COG0180. LUCA.
GeneTreeiENSGT00390000000666.
HOGENOMiHOG000224742.
HOVERGENiHBG002325.
InParanoidiP17248.
KOiK01867.
OMAiFFFSHRD.
OrthoDBiEOG7T4MK3.
TreeFamiTF105669.

Miscellaneous databases

NextBioi20805525.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR002306. Trp-tRNA-ligase.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mammalian tryptophanyl-tRNA synthetase shows little homology to prokaryotic synthetases but near identity with mammalian peptide chain release factor."
    Garret M., Pajot B., Trezeguet V., Labouesse J., Merle M., Gandar J.-C., Benedetto J.-P., Sallafranque M.-L., Alterio J., Gueguen M., Sarger C., Labouesse B., Bonnet J.
    Biochemistry 30:7809-7817(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Pancreas.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-475.
    Tissue: Pancreas.
  4. "Amino acid sequence of various peptides of tryptophanyl-tRNA-synthetase from bovine pancreas."
    Zargarova T.A., Kovaleva G.K., Favorova O.O., Levina N.B., Telezhinskaia I.N.
    Bioorg. Khim. 15:1307-1312(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 112-124; 337-354 AND 424-476.
    Tissue: Pancreas.

Entry informationi

Entry nameiSYWC_BOVIN
AccessioniPrimary (citable) accession number: P17248
Secondary accession number(s): Q3SZK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: July 11, 2006
Last modified: December 9, 2015
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.