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Protein

Transforming growth factor beta-1

Gene

Tgfb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-2129379. Molecules associated with elastic fibres.
R-RNO-2173788. Downregulation of TGF-beta receptor signaling.
R-RNO-2173789. TGF-beta receptor signaling activates SMADs.
R-RNO-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-RNO-3000170. Syndecan interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming growth factor beta-1
Short name:
TGF-beta-1
Cleaved into the following chain:
Gene namesi
Name:Tgfb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi69051. Tgfb1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • blood microparticle Source: AgBase
  • cell surface Source: BHF-UCL
  • cytoplasm Source: RGD
  • extracellular matrix Source: RGD
  • extracellular space Source: RGD
  • microvillus Source: Ensembl
  • neuronal cell body Source: RGD
  • nucleus Source: UniProtKB
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
  • secretory granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929By similarityAdd
BLAST
Chaini30 – 278249Latency-associated peptidePRO_0000033770Add
BLAST
Chaini279 – 390112Transforming growth factor beta-1PRO_0000033771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 – 33Interchain (with C-1350 or C-1375 in LTBP1); in inactive formBy similarity
Glycosylationi82 – 821N-linked (GlcNAc...)By similarity
Glycosylationi136 – 1361N-linked (GlcNAc...)By similarity
Glycosylationi176 – 1761N-linked (GlcNAc...)By similarity
Disulfide bondi223 – 223Interchain (with C-225)By similarity
Disulfide bondi225 – 225Interchain (with C-223)By similarity
Disulfide bondi285 ↔ 294By similarity
Disulfide bondi293 ↔ 356By similarity
Disulfide bondi322 ↔ 387By similarity
Disulfide bondi326 ↔ 389By similarity
Disulfide bondi355 – 355InterchainBy similarity

Post-translational modificationi

Glycosylated.By similarity
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP17246.
PRIDEiP17246.

PTM databases

PhosphoSiteiP17246.

Expressioni

Tissue specificityi

Abundant in the bone matrix.1 Publication

Gene expression databases

BgeeiENSRNOG00000020652.
GenevisibleiP17246. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, or heterodimer with TGFB2 (By similarity). Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3. Interacts with CD109 and DPT. Interacts with ASPN. May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Latency-associated peptide interacts with NREP; the interaction results in a decrease in TGFB1 autoinduction (By similarity).By similarity

GO - Molecular functioni

  • cytokine activity Source: GO_Central
  • glycoprotein binding Source: AgBase
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD
  • transforming growth factor beta receptor binding Source: RGD
  • type III transforming growth factor beta receptor binding Source: AgBase
  • type II transforming growth factor beta receptor binding Source: UniProtKB
  • type I transforming growth factor beta receptor binding Source: AgBase

Protein-protein interaction databases

BioGridi248721. 1 interaction.
DIPiDIP-6247N.
STRINGi10116.ENSRNOP00000028051.

Structurei

3D structure databases

ProteinModelPortaliP17246.
SMRiP17246. Positions 279-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 7445Straightjacket domainBy similarityAdd
BLAST
Regioni75 – 271197Arm domainBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi244 – 2463Cell attachment siteSequence analysis

Domaini

The 'straitjacket' and 'arm' domains encircle the growth factor monomers and are fastened together by strong bonding between Lys-56 and Tyr-103/Tyr-104. Activation of TGF-beta1 requires the binding of integrin alpha-V to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-beta binding proteins. The sheer physical force unfastens the straitjacket and releases the active growth factor dimer (By similarity).By similarity

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000119112.
HOGENOMiHOG000290198.
HOVERGENiHBG074115.
InParanoidiP17246.
KOiK13375.
OMAiSHSIYMF.
OrthoDBiEOG091G0BMM.
PhylomeDBiP17246.
TreeFamiTF318514.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR016319. TGF-beta.
IPR015615. TGF-beta-rel.
IPR003939. TGFb1.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PIRSFiPIRSF001787. TGF-beta. 1 hit.
PRINTSiPR01423. TGFBETA.
PR01424. TGFBETA1.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17246-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPSGLRLLP LLLPLPWLLV LTPGRPAAGL STCKTIDMEL VKRKRIEAIR
60 70 80 90 100
GQILSKLRLA SPPSQGEVPP GPLPEAVLAL YNSTRDRVAG ESADPEPEPE
110 120 130 140 150
ADYYAKEVTR VLMVDRNNAI YDKTKDITHS IYMFFNTSDI REAVPEPPLL
160 170 180 190 200
SRAELRLQRF KSTVEQHVEL YQKYSNNSWR YLGNRLLTPT DTPEWLSFDV
210 220 230 240 250
TGVVRQWLNQ GDGIQGFRFS AHCSCDSKDN VLHVEINGIS PKRRGDLGTI
260 270 280 290 300
HDMNRPFLLL MATPLERAQH LHSSRHRRAL DTNYCFSSTE KNCCVRQLYI
310 320 330 340 350
DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA
360 370 380 390
SASPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS
Length:390
Mass (Da):44,329
Last modified:August 1, 1990 - v1
Checksum:i5E21108ED50D853C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52498 mRNA. Translation: CAA36741.1.
AY550025 mRNA. Translation: AAS55640.1.
BC076380 mRNA. Translation: AAH76380.1.
PIRiS10219.
RefSeqiNP_067589.1. NM_021578.2.
UniGeneiRn.40136.

Genome annotation databases

EnsembliENSRNOT00000028051; ENSRNOP00000028051; ENSRNOG00000020652.
GeneIDi59086.
KEGGirno:59086.
UCSCiRGD:69051. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52498 mRNA. Translation: CAA36741.1.
AY550025 mRNA. Translation: AAS55640.1.
BC076380 mRNA. Translation: AAH76380.1.
PIRiS10219.
RefSeqiNP_067589.1. NM_021578.2.
UniGeneiRn.40136.

3D structure databases

ProteinModelPortaliP17246.
SMRiP17246. Positions 279-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248721. 1 interaction.
DIPiDIP-6247N.
STRINGi10116.ENSRNOP00000028051.

PTM databases

PhosphoSiteiP17246.

Proteomic databases

PaxDbiP17246.
PRIDEiP17246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028051; ENSRNOP00000028051; ENSRNOG00000020652.
GeneIDi59086.
KEGGirno:59086.
UCSCiRGD:69051. rat.

Organism-specific databases

CTDi7040.
RGDi69051. Tgfb1.

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000119112.
HOGENOMiHOG000290198.
HOVERGENiHBG074115.
InParanoidiP17246.
KOiK13375.
OMAiSHSIYMF.
OrthoDBiEOG091G0BMM.
PhylomeDBiP17246.
TreeFamiTF318514.

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-2129379. Molecules associated with elastic fibres.
R-RNO-2173788. Downregulation of TGF-beta receptor signaling.
R-RNO-2173789. TGF-beta receptor signaling activates SMADs.
R-RNO-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-RNO-3000170. Syndecan interactions.

Miscellaneous databases

PROiP17246.

Gene expression databases

BgeeiENSRNOG00000020652.
GenevisibleiP17246. RN.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR016319. TGF-beta.
IPR015615. TGF-beta-rel.
IPR003939. TGFb1.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PIRSFiPIRSF001787. TGF-beta. 1 hit.
PRINTSiPR01423. TGFBETA.
PR01424. TGFBETA1.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGFB1_RAT
AccessioniPrimary (citable) accession number: P17246
Secondary accession number(s): Q53YM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 7, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.