ID   PSAI_PEA                Reviewed;          40 AA.
AC   P17227;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 2.
DT   24-JAN-2024, entry version 110.
DE   RecName: Full=Photosystem I reaction center subunit VIII;
DE            Short=PSI-I;
GN   Name=psaI;
OS   Pisum sativum (Garden pea) (Lathyrus oleraceus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Alaska;
RX   PubMed=1807835; DOI=10.1007/bf00317074;
RA   Nagano Y., Matsuno R., Sasaki Y.;
RT   "Sequence and transcriptional analysis of the gene cluster trnQ-zfpA-psaI-
RT   ORF231-petA in pea chloroplasts.";
RL   Curr. Genet. 20:431-436(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Alaska;
RX   PubMed=1913879; DOI=10.1007/bf00309603;
RA   Smith A.G., Wilson R.J., Kaethner T.M., Willey D.L., Gray J.C.;
RT   "Pea chloroplast genes encoding a 4 kDa polypeptide of photosystem I and a
RT   putative enzyme of C1 metabolism.";
RL   Curr. Genet. 19:403-410(1991).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-16.
RX   PubMed=2185953; DOI=10.1016/0014-5793(90)81391-z;
RA   Ikeuchi M., Hirano A., Hiyama T., Inoue Y.;
RT   "Polypeptide composition of higher plant photosystem I complex.
RT   Identification of psaI, psaJ and psaK gene products.";
RL   FEBS Lett. 263:274-278(1990).
RN   [4]
RP   PROTEIN SEQUENCE OF 3-25.
RX   PubMed=8262226; DOI=10.1016/0014-5793(93)81617-9;
RA   Zakharov S.D., Ewy R.G., Dilley R.A.;
RT   "Subunit III of the chloroplast ATP-synthase can form a Ca(2+)-binding site
RT   on the lumenal side of the thylakoid membrane.";
RL   FEBS Lett. 336:95-99(1993).
CC   -!- FUNCTION: May help in the organization of the PsaL subunit.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PsaI family. {ECO:0000305}.
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DR   EMBL; X56315; CAA39757.1; -; Genomic_DNA.
DR   EMBL; X54750; CAA38547.1; -; Genomic_DNA.
DR   PIR; S17921; S17921.
DR   RefSeq; YP_003587557.1; NC_014057.1.
DR   PDB; 2O01; X-ray; 3.40 A; I=1-30.
DR   PDB; 2WSC; X-ray; 3.30 A; I=1-40.
DR   PDB; 2WSE; X-ray; 3.49 A; I=1-40.
DR   PDB; 2WSF; X-ray; 3.48 A; I=1-40.
DR   PDB; 3LW5; X-ray; 3.30 A; I=1-30.
DR   PDB; 4RKU; X-ray; 3.00 A; I=5-30.
DR   PDB; 4XK8; X-ray; 2.80 A; I/i=2-31.
DR   PDB; 4Y28; X-ray; 2.80 A; I=3-32.
DR   PDB; 5L8R; X-ray; 2.60 A; I=1-40.
DR   PDB; 6LY5; EM; 2.38 A; i=1-40.
DR   PDB; 6YAC; EM; 2.50 A; I=2-32.
DR   PDB; 6YEZ; EM; 2.70 A; I=2-32.
DR   PDB; 6ZOO; EM; 2.74 A; I=2-32.
DR   PDB; 6ZXS; X-ray; 3.00 A; I=3-32.
DR   PDB; 7DKZ; X-ray; 2.39 A; I=1-40.
DR   PDBsum; 2O01; -.
DR   PDBsum; 2WSC; -.
DR   PDBsum; 2WSE; -.
DR   PDBsum; 2WSF; -.
DR   PDBsum; 3LW5; -.
DR   PDBsum; 4RKU; -.
DR   PDBsum; 4XK8; -.
DR   PDBsum; 4Y28; -.
DR   PDBsum; 5L8R; -.
DR   PDBsum; 6LY5; -.
DR   PDBsum; 6YAC; -.
DR   PDBsum; 6YEZ; -.
DR   PDBsum; 6ZOO; -.
DR   PDBsum; 6ZXS; -.
DR   PDBsum; 7DKZ; -.
DR   AlphaFoldDB; P17227; -.
DR   EMDB; EMD-10746; -.
DR   EMDB; EMD-10798; -.
DR   EMDB; EMD-11326; -.
DR   SMR; P17227; -.
DR   DIP; DIP-60289N; -.
DR   IntAct; P17227; 3.
DR   GeneID; 9073045; -.
DR   BRENDA; 1.97.1.12; 4872.
DR   EvolutionaryTrace; P17227; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009522; C:photosystem I; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00431; PSI_PsaI; 1.
DR   InterPro; IPR001302; PSI_PsaI.
DR   InterPro; IPR036357; PSI_PsaI_sf.
DR   NCBIfam; TIGR03052; PS_I_psaI; 1.
DR   PANTHER; PTHR35775; -; 1.
DR   PANTHER; PTHR35775:SF2; PHOTOSYSTEM I REACTION CENTER SUBUNIT VIII; 1.
DR   Pfam; PF00796; PSI_8; 1.
DR   SUPFAM; SSF81540; Subunit VIII of photosystem I reaction centre, PsaI; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chloroplast; Direct protein sequencing; Membrane;
KW   Photosynthesis; Photosystem I; Plastid; Thylakoid; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..40
FT                   /note="Photosystem I reaction center subunit VIII"
FT                   /id="PRO_0000194668"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        13
FT                   /note="G -> S (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        22
FT                   /note="A -> R (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   HELIX           5..13
FT                   /evidence="ECO:0007829|PDB:6LY5"
FT   HELIX           15..28
FT                   /evidence="ECO:0007829|PDB:6LY5"
SQ   SEQUENCE   40 AA;  4470 MW;  3CE0B06A11A58E48 CRC64;
     MINLPSLFVP LVGLLFPAVA MASLFLHVEK RLLFSTKKIN
//