P17227 (PSAI_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photosystem I reaction center subunit VIII
Short name=PSI-I

Gene names

Name:

psaI

Encoded on

Plastid; Chloroplast

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	40 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May help in the organization of the PsaL subunit. HAMAP-Rule MF_00431
Subcellular location	Plastid › chloroplast thylakoid membrane; Single-pass membrane protein By similarity HAMAP-Rule MF_00431.
Sequence similarities	Belongs to the PsaI family.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem I Plastid Thylakoid
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW photosystem I Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 40

40

Photosystem I reaction center subunit VIII HAMAP-Rule MF_00431

PRO_0000194668

Regions

Transmembrane

8 – 28

21

Helical; Potential

Experimental info

Sequence conflict

13

1

G → S AA sequence Ref.4

Sequence conflict

22

1

A → R AA sequence Ref.4

Secondary structure

1

.

.

.

.

.

.

40

Helix Strand Turn

Details...

Turn

6 – 9

4

Helix

10 – 14

5

Helix

15 – 23

9

Turn

24 – 28

5

Sequences

	Sequence		Length	Mass (Da)
	P17227 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: 3CE0B06A11A58E48	FASTA	40	4,470
	10 20 30 40 MINLPSLFVP LVGLLFPAVA MASLFLHVEK RLLFSTKKIN

References

[1]	"Sequence and transcriptional analysis of the gene cluster trnQ-zfpA-psaI-ORF231-petA in pea chloroplasts." Nagano Y., Matsuno R., Sasaki Y. Curr. Genet. 20:431-436(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Alaska.
[2]	"Pea chloroplast genes encoding a 4 kDa polypeptide of photosystem I and a putative enzyme of C1 metabolism." Smith A.G., Wilson R.J., Kaethner T.M., Willey D.L., Gray J.C. Curr. Genet. 19:403-410(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Alaska.
[3]	"Polypeptide composition of higher plant photosystem I complex. Identification of psaI, psaJ and psaK gene products." Ikeuchi M., Hirano A., Hiyama T., Inoue Y. FEBS Lett. 263:274-278(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-16.
[4]	"Subunit III of the chloroplast ATP-synthase can form a Ca(2+)-binding site on the lumenal side of the thylakoid membrane." Zakharov S.D., Ewy R.G., Dilley R.A. FEBS Lett. 336:95-99(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-25.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56315 Genomic DNA. Translation: CAA39757.1.
X54750 Genomic DNA. Translation: CAA38547.1.

PIR

S17921.

RefSeq

YP_003587557.1. NC_014057.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YO9	model	-	I	1-40	[»]
2O01	X-ray	3.40	I	1-30	[»]
2WSC	X-ray	3.30	I	1-40	[»]
2WSE	X-ray	3.49	I	1-40	[»]
2WSF	X-ray	3.48	I	1-40	[»]
3LW5	X-ray	3.30	I	1-30	[»]

ProteinModelPortal

P17227.

SMR

P17227. Positions 1-30.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

9073045.

Family and domain databases

HAMAP

MF_00431. PSI_PsaI.

InterPro

IPR001302. PSI_PsaI.
[Graphical view]

Pfam

PF00796. PSI_8. 1 hit.
[Graphical view]

ProDom

PD003995. PSI_su8. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF81540. PSI_su8. 1 hit.

TIGRFAMs

TIGR03052. PS_I_psaI. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P17227.

Entry information

Entry name

PSAI_PEA

Accession

Primary (citable) accession number: P17227

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program