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Protein

Polypyrimidine tract-binding protein 1

Gene

Ptbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 1
Short name:
PTB
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein I
Short name:
hnRNP I
Gene namesi
Name:Ptbp1
Synonyms:Ptb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97791. Ptbp1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817381 – 527Polypyrimidine tract-binding protein 1Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei16PhosphoserineCombined sources1
Modified residuei126PhosphotyrosineBy similarity1
Modified residuei137PhosphothreonineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei430PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP17225.
MaxQBiP17225.
PeptideAtlasiP17225.
PRIDEiP17225.
TopDownProteomicsiP17225.

PTM databases

iPTMnetiP17225.
PhosphoSitePlusiP17225.
SwissPalmiP17225.

Expressioni

Tissue specificityi

Expressed in myoblast; expression gradually decreases during muscle cell differentiation (at protein level).1 Publication

Gene expression databases

CleanExiMM_PTBP1.

Interactioni

Subunit structurei

Monomer. Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1. Interacts with SFPQ (By similarity). Interacts with RAVER1.By similarity1 Publication

Protein-protein interaction databases

IntActiP17225. 7 interactors.
MINTiMINT-1862139.

Structurei

3D structure databases

ProteinModelPortaliP17225.
SMRiP17225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 142RRM 1PROSITE-ProRule annotationAdd BLAST85
Domaini183 – 259RRM 2PROSITE-ProRule annotationAdd BLAST77
Domaini335 – 386RRM 3PROSITE-ProRule annotationAdd BLAST52
Domaini450 – 525RRM 4PROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi315 – 321Poly-Ala7

Domaini

The C-terminal 195 amino acids of PTB are sufficient for specific RNA binding.

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiP17225.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGIVPDIAV GTKRGSDELF STCVSNGPFI MSSSASAANG NDSKKFKGDN
60 70 80 90 100
RSAGVPSRVI HVRKLPSDVT EGEVISLGLP FGKVTNLLML KGKNQAFIEM
110 120 130 140 150
NTEEAANTMV NYYTSVAPVL RGQPIYIQFS NHKELKTDSS PNQVRAQAAL
160 170 180 190 200
QAVNSVQSGN LALAASAAAV DAGMAMAGQS PVLRIIVENL FYPVTLDVLH
210 220 230 240 250
QIFSKFGTVL KIITFTKNNQ FQALLQYADP VSAQHAKLSL DGQNIYNACC
260 270 280 290 300
TLRIDFSKLT SLNVKYNNDK SRDYTRPDLP SGDSQPSLDQ TMAAAFGLSV
310 320 330 340 350
PNVHGALAPL AIPSAAAAAA ASRIAIPGLA GAGNSVLLVS NLNPERVTPQ
360 370 380 390 400
SLFILFGVYG DVQRVKILFN KKENALVQMA DGSQAQLGEP PERAQAAREV
410 420 430 440 450
SAHYTVQASE CAAAREGQED QGLTKDYGSS PLRFKKPGSK NFQNIFPPSA
460 470 480 490 500
TLHLSNIPPS VSEDDLKSLF SSNGGVVKGF KFFQKDRKMA LIQMGSVEEA
510 520
VQALIELHNH DLGENHHLRV SFSKSTI
Length:527
Mass (Da):56,478
Last modified:August 1, 1992 - v2
Checksum:iF18FDF376010D76A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52101 mRNA. Translation: CAA36321.1.
UniGeneiMm.265610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52101 mRNA. Translation: CAA36321.1.
UniGeneiMm.265610.

3D structure databases

ProteinModelPortaliP17225.
SMRiP17225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP17225. 7 interactors.
MINTiMINT-1862139.

PTM databases

iPTMnetiP17225.
PhosphoSitePlusiP17225.
SwissPalmiP17225.

Proteomic databases

EPDiP17225.
MaxQBiP17225.
PeptideAtlasiP17225.
PRIDEiP17225.
TopDownProteomicsiP17225.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:97791. Ptbp1.

Phylogenomic databases

HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiP17225.

Miscellaneous databases

ChiTaRSiPtbp1. mouse.
PROiP17225.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PTBP1.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTBP1_MOUSE
AccessioniPrimary (citable) accession number: P17225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.