Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type I restriction enzyme EcoprrI

Gene

prrD

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Putative 'masking-agent' for the anticodon nuclease. Recognizes the double-stranded sequence 5'-CCAN7RTGC-3'.

Catalytic activityi

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

REBASEi2541. EcoprrI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type I restriction enzyme EcoprrI (EC:3.1.21.3)
Gene namesi
Name:prrD
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Type I restriction enzyme EcoprrIPRO_0000077261Add
BLAST

Proteomic databases

PRIDEiP17224.

Structurei

3D structure databases

ProteinModelPortaliP17224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HsdR family.Curated

Family and domain databases

InterProiIPR007409. Restrct_endonuc_type1_HsdR_N.
[Graphical view]
PfamiPF04313. HSDR_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDCTKPIAE SNNFIILDKY NPDWKITESY QSEGDLEREL IQDLVNQGYE
60 70 80 90 100
YLPTLNNTKA MLANVREQLQ NLNNVEFLEA EWRRFVETWM DKPSDGVVEK
110 120 130 140 150
ARKIHDDYVH DFVFDDGRIQ NIYLLDRKNI LRNKVQVIKQ FEQAGTHANR
160 170 180 190 200
YDVTILVNGL PLVQIELKKR GVAIREAFNQ IHRYSKESFN SENSLFKYLQ
210 220 230 240 250
LFVISNGTDT RYFANTTKRD KNSFDFTMNW AKSDNTLIKD LKDFTATFFQ
260 270 280 290 300
KHTLLNVLVN YSVFDSSQTL LVMRPYQIAA TERILWKIKS SFTSEELVKT
310
GKRWVYLAHY RFW
Length:313
Mass (Da):37,036
Last modified:August 1, 1990 - v1
Checksum:iFB2628D6630ED828
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52284 Genomic DNA. Translation: CAA36528.1.
PIRiS09628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52284 Genomic DNA. Translation: CAA36528.1.
PIRiS09628.

3D structure databases

ProteinModelPortaliP17224.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi2541. EcoprrI.

Proteomic databases

PRIDEiP17224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR007409. Restrct_endonuc_type1_HsdR_N.
[Graphical view]
PfamiPF04313. HSDR_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease."
    Levitz R., Chapman D., Amitsur M., Green R., Snyder L., Kaufmann G.
    EMBO J. 9:1383-1389(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CTR5X.

Entry informationi

Entry nameiT1RP_ECOLX
AccessioniPrimary (citable) accession number: P17224
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.