P17224 (T1RP_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type I restriction enzyme EcoprrI
EC=3.1.21.3

Gene names

Name:

prrD

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Putative 'masking-agent' for the anticodon nuclease. Recognizes the double-stranded sequence 5'-CCAN7RTGC-3'.
Catalytic activity	Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.
Sequence similarities	Belongs to the HsdR family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW Type I site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Type I restriction enzyme EcoprrI		PRO_0000077261

Sequences

	Sequence		Length	Mass (Da)
	P17224 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: FB2628D6630ED828	FASTA	313	37,036
	10 20 30 40 50 60 MVDCTKPIAE SNNFIILDKY NPDWKITESY QSEGDLEREL IQDLVNQGYE YLPTLNNTKA 70 80 90 100 110 120 MLANVREQLQ NLNNVEFLEA EWRRFVETWM DKPSDGVVEK ARKIHDDYVH DFVFDDGRIQ 130 140 150 160 170 180 NIYLLDRKNI LRNKVQVIKQ FEQAGTHANR YDVTILVNGL PLVQIELKKR GVAIREAFNQ 190 200 210 220 230 240 IHRYSKESFN SENSLFKYLQ LFVISNGTDT RYFANTTKRD KNSFDFTMNW AKSDNTLIKD 250 260 270 280 290 300 LKDFTATFFQ KHTLLNVLVN YSVFDSSQTL LVMRPYQIAA TERILWKIKS SFTSEELVKT 310 GKRWVYLAHY RFW

References

[1]	"The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease." Levitz R., Chapman D., Amitsur M., Green R., Snyder L., Kaufmann G. EMBO J. 9:1383-1389(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CTR5X.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52284 Genomic DNA. Translation: CAA36528.1.
PIR	S09628.
3D structure databases
ProteinModelPortal	P17224.
ModBase	Search...
Protein family/group databases
REBASE	2541. EcoprrI.
Proteomic databases
PRIDE	P17224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007409. Restrct_endonuc_type1_HsdR_N. [Graphical view]
Pfam	PF04313. HSDR_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1RP_ECOLX

Accession

Primary (citable) accession number: P17224

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program