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Protein

Sex-determining protein fem-1

Gene

fem-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sex-determining protein; essential for the adoption of the male sexual fate in all tissues. Substrate recognition subunit of the cullin-RING-based CBC(fem-1) (Cul2-ElonginB-ElonginC) E3 ubiquitin-protein ligase complex which mediates in association with cofactors fem-2 and fem-3 the ubiquitination and subsequent proteasomal degradation of tra-1.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • male germ-line sex determination Source: WormBase
  • male somatic sex determination Source: WormBase
  • masculinization of hermaphroditic germ-line Source: WormBase
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of gene expression Source: UniProtKB
  • regulation of germ cell proliferation Source: UniProtKB
  • transcription factor catabolic process Source: WormBase

Keywordsi

Molecular functionDevelopmental protein
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-CEL-8951664. Neddylation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Sex-determining protein fem-1
Alternative name(s):
Feminization of XX and XO animals protein 1
Gene namesi
Name:fem-1
Synonyms:isx-1
ORF Names:F35D6.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF35D6.1a; CE07175; WBGene00001411; fem-1.
F35D6.1b; CE28299; WBGene00001411; fem-1.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi320D → A: No ced-3-mediated cleavage. 1 Publication1
Mutagenesisi344D → A: Does not affect ced-3-mediated cleavage. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670581 – 656Sex-determining protein fem-1Add BLAST656

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei320Cleavage; by ced-31 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP17221.
PaxDbiP17221.
PeptideAtlasiP17221.

Miscellaneous databases

PMAP-CutDBiP17221.

Expressioni

Gene expression databases

BgeeiWBGene00001411.

Interactioni

Subunit structurei

Component of the CBC(fem-1) E3 ubiquitin-protein ligase complex containing cul-2, elc-1, rbx-1, fem-1 and also associated fem-2 and fem-3 (PubMed:17609115). Interacts with cul-2. Interacts with fem-2 and fem-3 (PubMed:17609115). Interacts with sel-10 (PubMed:15306688). Interacts with ced-4 (PubMed:10764728).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi42457. 9 interactors.
IntActiP17221. 7 interactors.
MINTiMINT-128870.
STRINGi6239.F35D6.1a.

Structurei

3D structure databases

ProteinModelPortaliP17221.
SMRiP17221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati47 – 76ANK 1Add BLAST30
Repeati88 – 118ANK 2Add BLAST31
Repeati122 – 151ANK 3Add BLAST30
Repeati155 – 184ANK 4Add BLAST30
Repeati188 – 217ANK 5Add BLAST30
Repeati220 – 250ANK 6Add BLAST31
Repeati346 – 379TPRAdd BLAST34
Repeati557 – 588ANK 7Add BLAST32

Sequence similaritiesi

Belongs to the fem-1 family.Curated

Keywords - Domaini

ANK repeat, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0508. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00900000140956.
HOGENOMiHOG000008180.
InParanoidiP17221.
OMAiALKYWKR.
OrthoDBiEOG091G03I9.
PhylomeDBiP17221.

Family and domain databases

CDDicd00204. ANK. 1 hit.
Gene3Di1.10.10.10. 1 hit.
1.25.40.20. 4 hits.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR036770. Ankyrin_rpt-contain_sf.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF12796. Ank_2. 2 hits.
SMARTiView protein in SMART
SM00248. ANK. 7 hits.
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 2 hits.
PS50088. ANK_REPEAT. 4 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P17221-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTPNGHHFRT VIYNAAAVGN LQRIKVFTIN SRNDRQWIID CFNSDQDGRY
60 70 80 90 100
PLVIAARNGH ANVVEYLLEI GADPSVRGVV EFDNENIQGT PPLWAASAAG
110 120 130 140 150
HIEIVKLLIE KANADVNQAT NTRSTPLRGA CYDGHLDIVK YLLEKGADPH
160 170 180 190 200
IPNRHGHTCL MIASYRNKVG IVEELLKTGI DVNKKTERGN TALHDAAESG
210 220 230 240 250
NVEVVKILLK HGSVLMKDIQ GVDPLMGAAL SGFLDVLNVL ADQMPSGIHK
260 270 280 290 300
RDALKLLGST LLDKKMDAMS AMNCWKQSME VPLHADDLRL VREMETFFEP
310 320 330 340 350
QEVYEYQREA QNIAQVELLD GNIEAQRMQA LVIRERILGG AHTDVHYYLR
360 370 380 390 400
FRGAVYCDMG QMNRCYDLWK HALELQQKHF APLYYGTITT LQSFHETFSM
410 420 430 440 450
SLNDFVNNHH ANRNLRVRSS WVKYVFNGVC LELERAAAWT GAPLLEDTEC
460 470 480 490 500
CGKERCQHAT EESEYKKLVY VAVHLVNVLE RLSLPSAHID DSDEEKEPKA
510 520 530 540 550
DVRRLMIVCH ELHIPLLHHT LEERIPDSNS AELGLPKAAV LEQLLELDLD
560 570 580 590 600
VNATDKNNDT PMHILLRARE FRKSLVRALL VRGTWLFARN RHGDVVLNVM
610 620 630 640 650
KRMKALNHAN FDDLPLGRHI TLAGLVANAM RVKYPKKFVG VEEQMPLELR

RFYLAH
Length:656
Mass (Da):74,286
Last modified:August 1, 1990 - v1
Checksum:i4F5FC3FA3EA950D3
GO
Isoform b (identifier: P17221-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-104: QGTPPLWAASAAGHIEI → LFRRNTSFVGCLRCWTH
     105-656: Missing.

Note: No experimental confirmation available.
Show »
Length:104
Mass (Da):11,906
Checksum:i4C413AE3FC2DEB97
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01567688 – 104QGTPP…GHIEI → LFRRNTSFVGCLRCWTH in isoform b. CuratedAdd BLAST17
Alternative sequenceiVSP_015677105 – 656Missing in isoform b. CuratedAdd BLAST552

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03172 Genomic DNA. Translation: AAA28055.1.
FO080146 Genomic DNA. Translation: CCD61590.1.
FO080146 Genomic DNA. Translation: CCD61591.1.
PIRiA34793.
RefSeqiNP_500824.1. NM_068423.5. [P17221-1]
NP_500825.1. NM_068424.3. [P17221-2]
UniGeneiCel.5131.

Genome annotation databases

EnsemblMetazoaiF35D6.1a; F35D6.1a; WBGene00001411. [P17221-1]
GeneIDi177335.
KEGGicel:CELE_F35D6.1.
UCSCiF35D6.1a. c. elegans. [P17221-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFEM1_CAEEL
AccessioniPrimary (citable) accession number: P17221
Secondary accession number(s): Q95ZU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 25, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families