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Protein

Proteasome subunit alpha type-2

Gene

Psma2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_272498. APC/C:Cdc20 mediated degradation of Securin.
REACT_274511. Regulation of ornithine decarboxylase (ODC).
REACT_275019. Ubiquitin-dependent degradation of Cyclin D1.
REACT_277636. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_280071. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_280715. GLI3 is processed to GLI3R by the proteasome.
REACT_287195. Degradation of GLI2 by the proteasome.
REACT_288207. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_289020. Degradation of beta-catenin by the destruction complex.
REACT_290448. Orc1 removal from chromatin.
REACT_295138. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_297906. Separation of Sister Chromatids.
REACT_305105. degradation of DVL.
REACT_309656. SCF-beta-TrCP mediated degradation of Emi1.
REACT_312094. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_317312. SCF(Skp2)-mediated degradation of p27/p21.
REACT_318052. Activation of NF-kappaB in B cells.
REACT_325005. CDT1 association with the CDC6:ORC:origin complex.
REACT_327126. Hedgehog 'on' state.
REACT_333644. Asymmetric localization of PCP proteins.
REACT_334373. degradation of AXIN.
REACT_336450. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_336611. Hedgehog ligand biogenesis.
REACT_338477. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_343414. ER-Phagosome pathway.
REACT_346077. CDK-mediated phosphorylation and removal of Cdc6.
REACT_349901. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_357442. Degradation of GLI1 by the proteasome.
REACT_359556. CLEC7A (Dectin-1) signaling.
REACT_361793. Dectin-1 mediated noncanonical NF-kB signaling.

Protein family/group databases

MEROPSiT01.972.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-2 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit C3
Multicatalytic endopeptidase complex subunit C3
Proteasome component C3
Gene namesi
Name:Psma2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi61842. Psma2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication
  • CytoplasmP-body By similarity

  • Note: Colocalizes with TRIM5 in the cytoplasmic bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi121 – 1211Y → F: Abolishes nuclear localization and phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 234233Proteasome subunit alpha type-2PRO_0000124079Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei24 – 241PhosphotyrosineBy similarity
Modified residuei70 – 701N6-acetyllysineBy similarity
Modified residuei76 – 761PhosphotyrosineBy similarity
Modified residuei121 – 1211Phosphotyrosine1 Publication
Modified residuei171 – 1711N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues; which may be important for nuclear import.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP17220.

2D gel databases

World-2DPAGE0004:P17220.

PTM databases

PhosphoSiteiP17220.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

GenevestigatoriP17220.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Protein-protein interaction databases

BioGridi248290. 5 interactions.
IntActiP17220. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP17220.
SMRiP17220. Positions 2-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074870.
HOVERGENiHBG003005.
InParanoidiP17220.
KOiK02726.
OMAiWKATALG.
OrthoDBiEOG71VSTG.
PhylomeDBiP17220.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK
60 70 80 90 100
KQKSILYDER SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV
110 120 130 140 150
YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD
160 170 180 190 200
PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE
210 220 230
GQMTEDNIEV GICNEAGFRR LTPTEVRDYL AAIA
Length:234
Mass (Da):25,927
Last modified:January 23, 2007 - v3
Checksum:i4ECB56A233583821
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02897 mRNA. Translation: AAA40838.1.
PIRiA34535. SNRTC3.
RefSeqiNP_058975.1. NM_017279.1.
UniGeneiRn.1617.

Genome annotation databases

EnsembliENSRNOT00000073834; ENSRNOP00000066950; ENSRNOG00000049920.
GeneIDi29669.
KEGGirno:29669.
UCSCiRGD:61842. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02897 mRNA. Translation: AAA40838.1.
PIRiA34535. SNRTC3.
RefSeqiNP_058975.1. NM_017279.1.
UniGeneiRn.1617.

3D structure databases

ProteinModelPortaliP17220.
SMRiP17220. Positions 2-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248290. 5 interactions.
IntActiP17220. 1 interaction.

Protein family/group databases

MEROPSiT01.972.

PTM databases

PhosphoSiteiP17220.

2D gel databases

World-2DPAGE0004:P17220.

Proteomic databases

PRIDEiP17220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073834; ENSRNOP00000066950; ENSRNOG00000049920.
GeneIDi29669.
KEGGirno:29669.
UCSCiRGD:61842. rat.

Organism-specific databases

CTDi5683.
RGDi61842. Psma2.

Phylogenomic databases

GeneTreeiENSGT00550000074870.
HOVERGENiHBG003005.
InParanoidiP17220.
KOiK02726.
OMAiWKATALG.
OrthoDBiEOG71VSTG.
PhylomeDBiP17220.

Enzyme and pathway databases

ReactomeiREACT_272498. APC/C:Cdc20 mediated degradation of Securin.
REACT_274511. Regulation of ornithine decarboxylase (ODC).
REACT_275019. Ubiquitin-dependent degradation of Cyclin D1.
REACT_277636. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_279869. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_280071. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_280715. GLI3 is processed to GLI3R by the proteasome.
REACT_287195. Degradation of GLI2 by the proteasome.
REACT_288207. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_289020. Degradation of beta-catenin by the destruction complex.
REACT_290448. Orc1 removal from chromatin.
REACT_295138. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_297906. Separation of Sister Chromatids.
REACT_305105. degradation of DVL.
REACT_309656. SCF-beta-TrCP mediated degradation of Emi1.
REACT_312094. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_317312. SCF(Skp2)-mediated degradation of p27/p21.
REACT_318052. Activation of NF-kappaB in B cells.
REACT_325005. CDT1 association with the CDC6:ORC:origin complex.
REACT_327126. Hedgehog 'on' state.
REACT_333644. Asymmetric localization of PCP proteins.
REACT_334373. degradation of AXIN.
REACT_336450. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_336611. Hedgehog ligand biogenesis.
REACT_338477. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_343414. ER-Phagosome pathway.
REACT_346077. CDK-mediated phosphorylation and removal of Cdc6.
REACT_349901. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_357442. Degradation of GLI1 by the proteasome.
REACT_359556. CLEC7A (Dectin-1) signaling.
REACT_361793. Dectin-1 mediated noncanonical NF-kB signaling.

Miscellaneous databases

NextBioi609987.
PROiP17220.

Gene expression databases

GenevestigatoriP17220.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of cDNA for proteasomes from rat liver: primary structure of component C3 with a possible tyrosine phosphorylation site."
    Tanaka K., Fujiwara T., Kumatori A., Shin S., Yoshimura T., Ichihara A., Tokunaga F., Aruga R., Iwanaga S., Kakizuka A., Nakanishi S.
    Biochemistry 29:3777-3785(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Wistar.
    Tissue: Liver.
  2. "The NH2-terminal residues of rat liver proteasome (multicatalytic proteinase complex) subunits, C2, C3 and C8, are N alpha-acetylated."
    Tokunaga F., Aruga R., Iwanaga S., Tanaka K., Ichihara A., Takao T., Shimonishi Y.
    FEBS Lett. 263:373-375(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-30, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2.
    Tissue: Liver.
  3. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 5-39 AND 71-84, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  4. "Nuclear multicatalytic proteinase subunit RRC3 is important for growth regulation in hepatocytes."
    Benedict C.M., Clawson G.A.
    Biochemistry 35:11612-11621(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-121, PHOSPHORYLATION AT TYR-121.

Entry informationi

Entry nameiPSA2_RAT
AccessioniPrimary (citable) accession number: P17220
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.