Reviewed, UniProtKB/Swiss-Prot P17220 (PSA2_RAT)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-2
    EC=3.4.25.1
Alternative name(s):
    Proteasome component C3
    Macropain subunit C3
    Multicatalytic endopeptidase complex subunit C3

Gene names

Name:

Psma2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm. Nucleus Ref.4.
Tissue specificity	Ubiquitous.
Post-translational modification	Phosphorylated on tyrosine residues; which may be important for nuclear import. Ref.4
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 234	233	Proteasome subunit alpha type-2		PRO_0000124079
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.2
	Modified residue	7	1	Phosphoserine By similarity
	Modified residue	24	1	Phosphotyrosine By similarity
	Modified residue	57	1	Phosphotyrosine By similarity
	Modified residue	70	1	N6-acetyllysine By similarity
	Modified residue	76	1	Phosphotyrosine By similarity
	Modified residue	98	1	Phosphotyrosine By similarity
	Modified residue	121	1	Phosphotyrosine Probable
	Modified residue	171	1	N6-acetyllysine By similarity
Experimental info
	Mutagenesis	121	1	Y → F: Abolishes nuclear localization and phosphorylation. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P17220-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4ECB56A233583821	FASTA	234	25,927
	10 20 30 40 50 60 MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK KQKSILYDER 70 80 90 100 110 120 SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE 130 140 150 160 170 180 YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE 190 200 210 220 230 DLELEDAIHT AILTLKESFE GQMTEDNIEV GICNEAGFRR LTPTEVRDYL AAIA

References

[1]	"Molecular cloning of cDNA for proteasomes from rat liver: primary structure of component C3 with a possible tyrosine phosphorylation site." Tanaka K., Fujiwara T., Kumatori A., Shin S., Yoshimura T., Ichihara A., Tokunaga F., Aruga R., Iwanaga S., Kakizuka A., Nakanishi S. Biochemistry 29:3777-3785(1990) [PubMed: 2340272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Wistar. Tissue: Liver.
[2]	"The NH2-terminal residues of rat liver proteasome (multicatalytic proteinase complex) subunits, C2, C3 and C8, are N alpha-acetylated." Tokunaga F., Aruga R., Iwanaga S., Tanaka K., Ichihara A., Takao T., Shimonishi Y. FEBS Lett. 263:373-375(1990) [PubMed: 2335242] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-30. Tissue: Liver.
[3]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 5-39 AND 71-84, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
[4]	"Nuclear multicatalytic proteinase subunit RRC3 is important for growth regulation in hepatocytes." Benedict C.M., Clawson G.A. Biochemistry 35:11612-11621(1996) [PubMed: 8794741] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-121, PHOSPHORYLATION AT TYR-121.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02897 mRNA. Translation: AAA40838.1.
IPI	IPI00231757.
PIR	SNRTC3. A34535.
RefSeq	NP_058975.1.
UniGene	Rn.1617
3D structure databases
SMR	P17220. Positions 2-234.
ModBase	Search...
Protein family/group databases
MEROPS	T01.972.
PTM databases
PhosphoSite	P17220.
Proteomic databases
PRIDE	P17220.
Genome annotation databases
GeneID	29669.
KEGG	rno:29669.
Organism-specific databases
CTD	29669.
RGD	61842. Psma2.
Phylogenomic databases
eggNOG	maNOG09688.
HOVERGEN	P17220.
OrthoDB	EOG9BK7P5.
PhylomeDB	P17220.
Enzyme and pathway databases
BRENDA	3.4.25.1. 248.
Gene expression databases
Genevestigator	P17220.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_CS. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609987.

Entry information

Entry name

PSA2_RAT

Accession

Primary (citable) accession number: P17220

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)