P17214 (EST1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Telomere elongation protein EST1

Alternative name(s):
Ever shorter telomeres protein 1

Gene names

Name:	EST1
Ordered Locus Names:	YLR233C
ORF Names:	L8083.15

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Directly involved in telomere replication. Associates with telomerase and during its interaction with CDC13, telomerase activity is promoted. Ref.6 Ref.7
Subunit structure	Interacts with CDC13 and MPS3. Ref.6 Ref.7
Subcellular location	Nucleus. Chromosome › telomere Probable.
Sequence similarities	Belongs to the EST1 family.

Ontologies

Keywords
Cellular component	Chromosome Nucleus Telomere
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G-quadruplex DNA formation Inferred from direct assay. Source: SGD telomere maintenance via telomerase Inferred from mutant phenotype. Source: SGD
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay. Source: SGD telomerase holoenzyme complex Inferred from direct assay. Source: SGD
Molecular function	RNA binding Inferred from physical interaction. Source: SGD RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.7. Source: IntAct single-stranded DNA binding Inferred from direct assay. Source: SGD telomeric DNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC13	P32797	3	EBI-6684,EBI-4187
MPS3	P47069	3	EBI-6684,EBI-25811

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 699	699	Telomere elongation protein EST1		PRO_0000087072
Experimental info
	Sequence conflict	49	1	R → C in AAA66908. Ref.1
	Sequence conflict	85	1	L → V in AAA66908. Ref.1
	Sequence conflict	287	1	D → N in AAA66908. Ref.1
	Sequence conflict	351	1	M → I in AAA66908. Ref.1
	Sequence conflict	535	1	V → M in AAA66908. Ref.1
	Sequence conflict	571	1	Y → H in AAA66908. Ref.1
	Sequence conflict	579	1	N → D in AAA66908. Ref.1
	Sequence conflict	582	1	I → M in AAA66908. Ref.1
	Sequence conflict	665	1	R → K in AAA66908. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P17214 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 9CF552AFDA6BDEDE	FASTA	699	81,800
	10 20 30 40 50 60 MDNEEVNEEC MRLFFKNARA HLDKHLTSRL TCDENAYITF RCFLDGIHRK STRFLEELLL 70 80 90 100 110 120 KQENMYHNNN YERINDSVIP LVLKLLWLQI HEPTLQWFEH WFHDIMRLSN RRKFRVFRIF 130 140 150 160 170 180 QKKMIQFFKI THRYYYDIIE HLCAKYDMNS VISNALFAKL NLMQYTDGLS THEKIILNTS 190 200 210 220 230 240 NPLTFSIVIS LQRCVINLGS THFYKTLLNK PSNKPKSVEG FEKSIRYLNI ASLYLPAVGD 250 260 270 280 290 300 TYFQRAKIYL ITGKFSLYFF ELVRGALVRI PSKCALNNLK DFILTPDFPE RRRLMKKLAI 310 320 330 340 350 360 LVSKDLKGEK SFFEGQIVLQ FLSIVEHTLV PQSWNASRAS NCWLLKEHLQ MAALKYHSGN 370 380 390 400 410 420 INVILENLAA TMGSFDLMFT TRKSKEQKNK LKYADLSERQ VFFLDLSFDF IANIIDVVIK 430 440 450 460 470 480 PSWQKNMEDF RYLAIIRLLM CWIKSYRSIL QYTHRHRKFC TSFALLLNDL INSPLNCSGN 490 500 510 520 530 540 IYSHRPKRSY LFREDIIFRE FSCINFALTD FNDDYVYDSP DMINNIIGCP TLTKVLSPKE 550 560 570 580 590 600 ECVLRIRSII FSGMKFLEKN DTGVIWNASK YKFDLISPNI KIKRQIALSE ISSKINVKTQ 610 620 630 640 650 660 QERVVSSRKV EAKRDEQQRK RAGKIAVTEL EKQFANVRRT KKLSPLPEKD GVSSELVKHA 670 680 690 ASRGRKTITG PLSSDFLSYP DEAIDADEDI TVQVPDTPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mutant with a defect in telomere elongation leads to senescence in yeast." Lundblad V., Szostak J.W. Cell 57:633-643(1989) [PubMed: 2655926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"RNA-dependent polymerase motifs in EST1: tentative identification of a protein component of an essential yeast telomerase." Lundblad V., Blackburn E.H. Cell 60:529-530(1990) [PubMed: 2406024] [Abstract] Cited for: PUTATIVE SIMILARITY TO REVERSE TRANSCRIPTASES.
[5]	"Playing with blocks: some pitfalls of forcing multiple alignments." Henikoff S. New Biol. 3:1148-1154(1991) [PubMed: 1669287] [Abstract] Cited for: SHOWS THAT THIS SIMILARITY IS DOUBTFUL.
[6]	"New function of CDC13 in positive telomere length regulation." Meier B., Driller L., Jaklin S., Feldmann H.M. Mol. Cell. Biol. 21:4233-4245(2001) [PubMed: 11390652] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC13.
[7]	"The nuclear envelope and spindle pole body-associated Mps3 protein bind telomere regulators and function in telomere clustering." Antoniacci L.M., Kenna M.A., Skibbens R.V. Cell Cycle 6:75-79(2007) [PubMed: 17245108] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPS3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04849 Genomic DNA. Translation: AAA66908.1. U19027 Genomic DNA. Translation: AAB67418.1. BK006945 Genomic DNA. Translation: DAA09549.1.
PIR	S51454.
RefSeq	NP_013334.1. NM_001182120.1.
3D structure databases
ProteinModelPortal	P17214.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1306N.
IntAct	P17214. 26 interactions.
MINT	MINT-387253.
STRING	P17214.
Proteomic databases
PeptideAtlas	P17214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR233C; YLR233C; YLR233C.
GeneID	850934.
KEGG	sce:YLR233C.
NMPDR	fig|4932.3.peg.4347.
Organism-specific databases
CYGD	YLR233c.
SGD	S000004223. EST1.
Phylogenomic databases
eggNOG	fuNOG08065.
GeneTree	EFGT00050000008552.
OrthoDB	EOG4R273R.
Gene expression databases
ArrayExpress	P17214.
Genevestigator	P17214.
GermOnline	YLR233C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019458. EST1. IPR018834. Telomere_elong_Est1_DNA-bd. [Graphical view]
KO	K11132.
Pfam	PF10374. EST1. 1 hit. PF10373. EST1_DNA_bind. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967375.

Entry information

Entry name

EST1_YEAST

Accession

Primary (citable) accession number: P17214
Secondary accession number(s): D6VYN3, Q05997

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program