P17208 (PO4F1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
POU domain, class 4, transcription factor 1

Alternative name(s):
Brain-specific homeobox/POU domain protein 3A
Short name=Brain-3A
Short name=Brn-3A
Brn-3.0

Gene names

Name:	Pou4f1
Synonyms:	Brn-3, Brn3, Brn3a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Subcellular location	Nucleus.
Tissue specificity	Brain, peripheral sensory nervous system and retina. In the adult nervous system BRN-3.0 predominates in the medial habenula, superficial gray of the superior colliculus, red nucleus, mesencephalic nucleus of the trigeminal ganglion, nucleus ambiguus, inferior olivary nucleus, and peripheral sensory ganglia.
Sequence similarities	Belongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell migration in hindbrain Inferred from mutant phenotype PubMed 8955272. Source: MGI central nervous system neuron differentiation Inferred from mutant phenotype PubMed 8876243PubMed 8955272. Source: MGI habenula development Inferred from mutant phenotype PubMed 19906978. Source: MGI innervation Inferred from mutant phenotype PubMed 19906978. Source: MGI mesoderm development Inferred from mutant phenotype PubMed 19877281. Source: MGI negative regulation of neuron apoptotic process Inferred from mutant phenotype PubMed 20096094. Source: BHF-UCL negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 19877281. Source: BHF-UCL neuron fate specification Inferred from mutant phenotype PubMed 19877281PubMed 20096094. Source: BHF-UCL peripheral nervous system neuron development Inferred from mutant phenotype PubMed 17239249PubMed 19877281PubMed 20096094. Source: BHF-UCL positive regulation of apoptotic process Inferred from genetic interaction PubMed 15492043. Source: MGI proprioception involved in equilibrioception Inferred from mutant phenotype PubMed 8876243PubMed 8955272. Source: MGI regulation of neurogenesis Inferred from mutant phenotype PubMed 19877281. Source: MGI sensory system development Inferred from mutant phenotype PubMed 20096094. Source: BHF-UCL suckling behavior Inferred from mutant phenotype PubMed 8876243PubMed 8955272. Source: MGI trigeminal nerve development Inferred from mutant phenotype PubMed 19877281. Source: BHF-UCL ventricular compact myocardium morphogenesis Inferred from mutant phenotype PubMed 18368538. Source: UniProtKB
Cellular_component	neuron projection Inferred from direct assay PubMed 19906978. Source: MGI nuclear chromatin Inferred from direct assay PubMed 17196582PubMed 20096094. Source: BHF-UCL
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay PubMed 18368538. Source: UniProtKB RNA polymerase II distal enhancer sequence-specific DNA binding Inferred from direct assay PubMed 17196582PubMed 20096094. Source: BHF-UCL RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 20096094. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	POU domain, class 4, transcription factor 1		PRO_0000100737
Regions
	Domain	262 – 339	78	POU-specific
	DNA binding	357 – 416	60	Homeobox
	Motif	57 – 66	10	POU-IV box
	Compositional bias	100 – 108	9	Poly-His
	Compositional bias	130 – 187	58	Gly-rich
	Compositional bias	205 – 258	54	Ala-rich
Experimental info
	Sequence conflict	179	1	G → A in AAB30577. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P17208 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 64EC98E5C0CF79E2	FASTA	421	42,767
	10 20 30 40 50 60 MMSMNSKQPH FAMHPTLPEH KYPSLHSSSE AIRRACLPTP PLQSNLFASL DETLLARAEA 70 80 90 100 110 120 LAAVDIAVSQ GKSHPFKPDA TYHTMNSVPC TSTSTVPLAH HHHHHHHHQA LEPGDLLDHI 130 140 150 160 170 180 SSPSLALMAG AGGAGAAGGG GGAHDGPGGG GGPGGGGGPG GGGPGGGGGG GGPGGGGGGP 190 200 210 220 230 240 GGGLLGGSAH PHPHMHGLGH LSHPAAAAAM NMPSGLPHPG LVAAAAHHGA AAAAAAAAAG 250 260 270 280 290 300 QVAAASAAAA VVGAAGLASI CDSDTDPREL EAFAERFKQR RIKLGVTQAD VGSALANLKI 310 320 330 340 350 360 PGVGSLSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEG AQREKMNKPE LFNGGEKKRK 370 380 390 400 410 420 RTSIAAPEKR SLEAYFAVQP RPSSEKIAAI AEKLDLKKNV VRVWFCNQRQ KQKRMKFSAT Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chromosomal localization and sequences of the murine Brn-3 family of developmental control genes." Theil T., Zechner U., Klett C., Adolph S., Moeroey T. Cytogenet. Cell Genet. 66:267-271(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Cloning and sequencing of POU-boxes expressed in mouse testis." Goldsborough A., Ashworth A., Willison K.R. Nucleic Acids Res. 18:1634-1634(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 286-401. Strain: T6 / TW1. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S69350 Genomic DNA. Translation: AAB30577.2. AC121997 Genomic DNA. No translation available. X51959 mRNA. Translation: CAA36218.1.
IPI	IPI00136852.
RefSeq	NP_035273.3. NM_011143.4.
UniGene	Mm.246550.
3D structure databases
ProteinModelPortal	P17208.
SMR	P17208. Positions 266-416.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000060798.
PTM databases
PhosphoSite	P17208.
Proteomic databases
PaxDb	P17208.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000053016; ENSMUSP00000060798; ENSMUSG00000048349.
GeneID	18996.
KEGG	mmu:18996.
Organism-specific databases
CTD	5457.
MGI	MGI:102525. Pou4f1.
Phylogenomic databases
eggNOG	NOG289030.
GeneTree	ENSGT00700000104015.
HOGENOM	HOG000116305.
HOVERGEN	HBG031829.
InParanoid	P17208.
KO	K09366.
OMA	SINDSDT.
OrthoDB	EOG4PK29D.
Gene expression databases
Bgee	P17208.
CleanEx	MM_POU4F1.
Genevestigator	P17208.
GermOnline	ENSMUSG00000048349. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 1.10.260.40. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR010982. Lambda_DNA-bd_dom. IPR013847. POU. IPR000327. POU_specific. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PRINTS	PR00028. POUDOMAIN.
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF47413. Lambda_like_DNA. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	295398.
SOURCE	Search...

Entry information

Entry name

PO4F1_MOUSE

Accession

Primary (citable) accession number: P17208
Secondary accession number(s): E9QPT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program